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The map label for this gene is ptsN [H]

Identifier: 83721433

GI number: 83721433

Start: 536991

End: 537446

Strand: Reverse

Name: ptsN [H]

Synonym: BTH_I0484

Alternate gene names: 83721433

Gene position: 537446-536991 (Counterclockwise)

Preceding gene: 83721047

Following gene: 83720779

Centisome position: 14.11

GC content: 62.94

Gene sequence:

>456_bases
ATGAATCGCCTAGCCAAAATCCTGCCTTTGGAGAACGTCGTCATCGGCCTCTCCGTCACCAGCAAGAAACGCGTCTTCGA
ACAAGCCGGCCTGATCTTCGAGAATCAGAACGGCATCGCCCGCAGCACCGTCACCGACAATCTGTTCGCACGCGAGCGTC
TCGGTTCGACGGGCCTCGGCGAAGGCGTTGCCATTCCGCATGGCCGGATCAAGGGACTCAAGCACCCGCTCGCCGCATTC
GTGCGGCTCGCCGAGCCGATCCCGTTCGAGGCGCCGGACGGCCAGCCCGTGTCGCTGCTGATCTTCCTGCTCGTGCCCGA
GCAGGCGACGCAGGCGCACCTCGAAATCCTTTCCGAAATCGCCCAGTTGCTGTCCGATCGCGACACGCGCGAGCGCCTGC
ATACCGAGCCGGATCGCGACGAGTTGCATCGACTCCTGACTCAGTGGCAACCTTAA

Upstream 100 bases:

>100_bases
GCGCTTCACAGGTTTCCGTCGCCTAACGCCGTCCTTTTTGACCGACATGGAAAATCAGTCCGCCGCGAGGAGACCCCAGG
CCGCCCCTACGCCTGCCAAC

Downstream 100 bases:

>100_bases
GCTGATCGGGGCGGTTCGTTCCCCATTGCGTCGCGGCCCGCGCCGCCGAGCCGAGCCGCCCGCCGCCGGGCCGGCGCCGC
AACCGCCGTTGCAGCGTCAG

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugarphosphate [C]

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MNRLAKILPLENVVIGLSVTSKKRVFEQAGLIFENQNGIARSTVTDNLFARERLGSTGLGEGVAIPHGRIKGLKHPLAAF
VRLAEPIPFEAPDGQPVSLLIFLLVPEQATQAHLEILSEIAQLLSDRDTRERLHTEPDRDELHRLLTQWQP

Sequences:

>Translated_151_residues
MNRLAKILPLENVVIGLSVTSKKRVFEQAGLIFENQNGIARSTVTDNLFARERLGSTGLGEGVAIPHGRIKGLKHPLAAF
VRLAEPIPFEAPDGQPVSLLIFLLVPEQATQAHLEILSEIAQLLSDRDTRERLHTEPDRDELHRLLTQWQP
>Mature_151_residues
MNRLAKILPLENVVIGLSVTSKKRVFEQAGLIFENQNGIARSTVTDNLFARERLGSTGLGEGVAIPHGRIKGLKHPLAAF
VRLAEPIPFEAPDGQPVSLLIFLLVPEQATQAHLEILSEIAQLLSDRDTRERLHTEPDRDELHRLLTQWQP

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=149, Percent_Identity=35.5704697986577, Blast_Score=94, Evalue=5e-21,
Organism=Escherichia coli, GI1786951, Length=99, Percent_Identity=35.3535353535354, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI2367327, Length=119, Percent_Identity=32.7731092436975, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI1788726, Length=145, Percent_Identity=28.2758620689655, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 16824; Mature: 16824

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLAKILPLENVVIGLSVTSKKRVFEQAGLIFENQNGIARSTVTDNLFARERLGSTGLG
CCCHHHHCCCCCEEEEEECCHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCC
EGVAIPHGRIKGLKHPLAAFVRLAEPIPFEAPDGQPVSLLIFLLVPEQATQAHLEILSEI
CCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
AQLLSDRDTRERLHTEPDRDELHRLLTQWQP
HHHHCCCHHHHHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MNRLAKILPLENVVIGLSVTSKKRVFEQAGLIFENQNGIARSTVTDNLFARERLGSTGLG
CCCHHHHCCCCCEEEEEECCHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCC
EGVAIPHGRIKGLKHPLAAFVRLAEPIPFEAPDGQPVSLLIFLLVPEQATQAHLEILSEI
CCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
AQLLSDRDTRERLHTEPDRDELHRLLTQWQP
HHHHCCCHHHHHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein Npi-phosphohistidine; sugar [C]

Specific reaction: Protein Npi-phosphohistidine + sugar = protein histidine + sugarphosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]