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The map label for this gene is ntaB [H]

Identifier: 83721388

GI number: 83721388

Start: 821905

End: 822420

Strand: Reverse

Name: ntaB [H]

Synonym: BTH_I0713

Alternate gene names: 83721388

Gene position: 822420-821905 (Counterclockwise)

Preceding gene: 83721443

Following gene: 83720049

Centisome position: 21.59

GC content: 66.28

Gene sequence:

>516_bases
ATGAAACACGGACAGGCCAGCCCGCCAAATTTCGATGCCGCCGCGTTCCGCCAAGCGCTCGGTCAGTTCGCGACCGGCGT
GACCGTGATCACGACGCGCGCGCCGTCCGGCCAGTTGATCGGCATCACCGCCAGCTCGTTCAACTCGGTATCGCTCGATC
CGCCGCTCGTGCTGTGGAGCCTCGCGCACAAATCGGCGTCGATGTCGGTGTTCCGCACGAACAGCCATTATGTCGTCAAC
GTGCTGTCCGCGTCGCAGCACGATCTGTGCATGCGCTTCGCGACGCTCAAGGGCAACCGCTTCGAAGGCGTGTCGCACGC
GCAGGGCGACTCCGGCATGCCCGTCCTCGACGGCGCGCTCGCGTGGTTCGAATGTCACAACCGCAGCCGCTACGACGAAG
GCGATCACGTGATCTTCGTCGGCGAGGTCGAGCGCTGCGGCGTGCACCCCGACGCCGCAGCGCTCGCGCCGCTCGTGTTC
CAGAGCGGCAGCTTCCACGGCCTCACACGGCTTTGA

Upstream 100 bases:

>100_bases
TTCCGATGACTGCCCTGCTCACTTCCCGCCATTTCGCCCGCGGCGCCGCCGCCCGTTCCGCCCGCCGCCGCGCGCGCCCA
TCGCTCGAGGCCGTCACGCA

Downstream 100 bases:

>100_bases
CCGTGGGCCCCGTGCGCGCGAGCGCGACAGGCGGCCCCACGTCGTCCTTCAGCGTCTGCAGCACGATGTTCGAGCGGATG
TCGAGCACGCCGGGCGTCTT

Product: flavin reductase domain-containing protein

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MKHGQASPPNFDAAAFRQALGQFATGVTVITTRAPSGQLIGITASSFNSVSLDPPLVLWSLAHKSASMSVFRTNSHYVVN
VLSASQHDLCMRFATLKGNRFEGVSHAQGDSGMPVLDGALAWFECHNRSRYDEGDHVIFVGEVERCGVHPDAAALAPLVF
QSGSFHGLTRL

Sequences:

>Translated_171_residues
MKHGQASPPNFDAAAFRQALGQFATGVTVITTRAPSGQLIGITASSFNSVSLDPPLVLWSLAHKSASMSVFRTNSHYVVN
VLSASQHDLCMRFATLKGNRFEGVSHAQGDSGMPVLDGALAWFECHNRSRYDEGDHVIFVGEVERCGVHPDAAALAPLVF
QSGSFHGLTRL
>Mature_171_residues
MKHGQASPPNFDAAAFRQALGQFATGVTVITTRAPSGQLIGITASSFNSVSLDPPLVLWSLAHKSASMSVFRTNSHYVVN
VLSASQHDLCMRFATLKGNRFEGVSHAQGDSGMPVLDGALAWFECHNRSRYDEGDHVIFVGEVERCGVHPDAAALAPLVF
QSGSFHGLTRL

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081819, Length=161, Percent_Identity=32.2981366459627, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 18339; Mature: 18339

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHGQASPPNFDAAAFRQALGQFATGVTVITTRAPSGQLIGITASSFNSVSLDPPLVLWS
CCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEEE
LAHKSASMSVFRTNSHYVVNVLSASQHDLCMRFATLKGNRFEGVSHAQGDSGMPVLDGAL
HHCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
AWFECHNRSRYDEGDHVIFVGEVERCGVHPDAAALAPLVFQSGSFHGLTRL
HEEHHCCCCCCCCCCEEEEEECHHHCCCCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKHGQASPPNFDAAAFRQALGQFATGVTVITTRAPSGQLIGITASSFNSVSLDPPLVLWS
CCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEEE
LAHKSASMSVFRTNSHYVVNVLSASQHDLCMRFATLKGNRFEGVSHAQGDSGMPVLDGAL
HHCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
AWFECHNRSRYDEGDHVIFVGEVERCGVHPDAAALAPLVFQSGSFHGLTRL
HEEHHCCCCCCCCCCEEEEEECHHHCCCCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]