Click here to switch to the map view.

The map label for this gene is gltD [H]

Identifier: 83721325

GI number: 83721325

Start: 3450977

End: 3452443

Strand: Reverse

Name: gltD [H]

Synonym: BTH_I3013

Alternate gene names: 83721325

Gene position: 3452443-3450977 (Counterclockwise)

Preceding gene: 83719119

Following gene: 83720579

Centisome position: 90.63

GC content: 67.14

Gene sequence:

>1467_bases
ATGGGCAAGATCACCGGTTTTCTGGAGTACGAGCGCCGTCACGAGGCGTACGAAGCGCCGCTCACGCGCGTGAAGCACTA
CAAGGAATTCGTCGCGGCGCTGACCGACGAGGACGCGAAGATCCAGGGCGCGCGCTGCATGGATTGCGGCATTCCGTTCT
GCAACAACGGCTGTCCGGTCAACAACATCATTCCGGACTTCAACGATCTCGTGTATCGCCAGGACTGGCGGCAGGCGATC
GACGTGCTGCACTCGACGAACAACTTCCCCGAGTTCACGGGCCGCATCTGCCCGGCGCCGTGCGAAGCGGCGTGCACGCT
CGGGATCAACGACGATCCGGTCGGCATCAAGTCGATCGAGCACGCGATCATCGACAAGGCGTGGGCGCAAGGCTGGGTCG
CGCCGCAGCCCGCCGCGCACAAGACGGGCAAGAAGGTCGCGGTGGTAGGCTCCGGCCCCGCGGGGCTCGCCGCCGCGCAG
CAGCTCGCGCGCGCGGGCCACGACGTGACGGTGTTCGAGAAGAACGACCGGATCGGCGGGCTGCTGCGCTACGGAATTCC
CGACTTCAAGCTCGAGAAGTGGCTGATCGACCGCCGGATGCGCCAGATGGAGGCCGAAGGCGTGACGTTCCGCACGAGCG
TGTTCATCGGCCGCGATCCGCTGCCCGAGTCGATCGGCAACATGGCGAAGGAGACGATTTCGCCCGAGACGCTGAAGGAC
GAATTCGACGCGGTGGTGATCGCGGGCGGCTCGGAGACGCCGCGCGATTTGCCGGTGCCGGGGCGCGAGCTCGCCGGCAT
CCATTTCGCGATGGAATTCCTGCCGCAGCAGAACCGCGTGAACGCGGGCGACAAGGTGGCCGATCAACTGCTCGCGAAGG
GCAAGCACGTCGTCGTGATCGGCGGCGGCGACACGGGCTCGGACTGTGTCGGCACGTCGAACCGCCACGGCGCGAAGCAC
ATCACGCAGTTCGAACTGCTGCCGCAGCCGCCCGAGGCGGAGAACAAGCCGCTCGTGTGGCCGTACTGGCCGATCAAGCT
GCGCACGTCGTCGTCGCACGAGGAAGGCTGCGAGCGCGACTGGGCGGTCGCGACGAAGCGTTTCGAGGGCAAGAACGGCA
AGGTCGAGAAGCTGATCGCGGTGCGCGTCGCGTGGGTCGACGGCAAGATGCAGGAAGTGCCGAACTCCGAGTTCGAGATC
AAGGCCGATCTCGTGCTGCTCGCGATGGGCTTCACGCAGCCGGTCGCGCCCGTGCTCGAGGCGTTCGGCGTCGCGAAGGA
CGCGCGCGGCAACGTGCGTGCGGGCACCGAGGGCGATCGTGCGTACTACACGTCGGTCGACAAGGTGTTCGCCGCGGGCG
ATATGCGCCGCGGCCAGTCGCTCGTCGTCTGGGCAATCCGCGAAGGCCGCCAGTGCGCGCGCTCGGTCGACGCGTACCTG
ATGGGCGGCTCCGAGTTGCCGCGTTGA

Upstream 100 bases:

>100_bases
GCCTGACGCAACGCGGCTCGACGACATAGCGAACGCGCCCGCCCGAACCGGCGGGCGCGGGTCCCCCTTACCCGATTCAA
CAGATTCAGAAGAGAACCTC

Downstream 100 bases:

>100_bases
GCGGGCGGCGTTCGCCAGAACGTCGTTTCGACGAACCGGGCGTCCCATGGGCGCCCGGTTTTTTTATGCGCGGACGCTTT
GCCGCGACGCGCATCGCAAG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MGKITGFLEYERRHEAYEAPLTRVKHYKEFVAALTDEDAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWRQAI
DVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIEHAIIDKAWAQGWVAPQPAAHKTGKKVAVVGSGPAGLAAAQ
QLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGRDPLPESIGNMAKETISPETLKD
EFDAVVIAGGSETPRDLPVPGRELAGIHFAMEFLPQQNRVNAGDKVADQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKH
ITQFELLPQPPEAENKPLVWPYWPIKLRTSSSHEEGCERDWAVATKRFEGKNGKVEKLIAVRVAWVDGKMQEVPNSEFEI
KADLVLLAMGFTQPVAPVLEAFGVAKDARGNVRAGTEGDRAYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
MGGSELPR

Sequences:

>Translated_488_residues
MGKITGFLEYERRHEAYEAPLTRVKHYKEFVAALTDEDAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWRQAI
DVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIEHAIIDKAWAQGWVAPQPAAHKTGKKVAVVGSGPAGLAAAQ
QLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGRDPLPESIGNMAKETISPETLKD
EFDAVVIAGGSETPRDLPVPGRELAGIHFAMEFLPQQNRVNAGDKVADQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKH
ITQFELLPQPPEAENKPLVWPYWPIKLRTSSSHEEGCERDWAVATKRFEGKNGKVEKLIAVRVAWVDGKMQEVPNSEFEI
KADLVLLAMGFTQPVAPVLEAFGVAKDARGNVRAGTEGDRAYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
MGGSELPR
>Mature_487_residues
GKITGFLEYERRHEAYEAPLTRVKHYKEFVAALTDEDAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWRQAID
VLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIEHAIIDKAWAQGWVAPQPAAHKTGKKVAVVGSGPAGLAAAQQ
LARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGRDPLPESIGNMAKETISPETLKDE
FDAVVIAGGSETPRDLPVPGRELAGIHFAMEFLPQQNRVNAGDKVADQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKHI
TQFELLPQPPEAENKPLVWPYWPIKLRTSSSHEEGCERDWAVATKRFEGKNGKVEKLIAVRVAWVDGKMQEVPNSEFEIK
ADLVLLAMGFTQPVAPVLEAFGVAKDARGNVRAGTEGDRAYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYLM
GGSELPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=488, Percent_Identity=24.3852459016393, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1789606, Length=486, Percent_Identity=34.156378600823, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI1788811, Length=467, Percent_Identity=34.0471092077088, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI87082180, Length=470, Percent_Identity=33.8297872340426, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1788468, Length=462, Percent_Identity=27.2727272727273, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789243, Length=445, Percent_Identity=27.6404494382022, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17570289, Length=506, Percent_Identity=45.8498023715415, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71984108, Length=478, Percent_Identity=25.5230125523013, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320030, Length=498, Percent_Identity=48.7951807228916, Blast_Score=469, Evalue=1e-133,
Organism=Drosophila melanogaster, GI28574881, Length=497, Percent_Identity=49.6981891348088, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665539, Length=497, Percent_Identity=49.6981891348088, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665547, Length=497, Percent_Identity=49.6981891348088, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665543, Length=497, Percent_Identity=49.6981891348088, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24640763, Length=482, Percent_Identity=23.6514522821577, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI18858217, Length=482, Percent_Identity=23.6514522821577, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53573; Mature: 53441

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKITGFLEYERRHEAYEAPLTRVKHYKEFVAALTDEDAKIQGARCMDCGIPFCNNGCPV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWRQAIDVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCHHHHH
HAIIDKAWAQGWVAPQPAAHKTGKKVAVVGSGPAGLAAAQQLARAGHDVTVFEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGRDPLPESIGNMAKETISPETLKD
EEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHC
EFDAVVIAGGSETPRDLPVPGRELAGIHFAMEFLPQQNRVNAGDKVADQLLAKGKHVVVI
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEE
GGGDTGSDCVGTSNRHGAKHITQFELLPQPPEAENKPLVWPYWPIKLRTSSSHEEGCERD
ECCCCCCCCCCCCCCCCCHHCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCHHHCCHH
WAVATKRFEGKNGKVEKLIAVRVAWVDGKMQEVPNSEFEIKADLVLLAMGFTQPVAPVLE
HHHHHHHCCCCCCCEEEEEEEEEEEECCHHHHCCCCCEEEEEEEEEEEECCCCHHHHHHH
AFGVAKDARGNVRAGTEGDRAYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
HHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHEE
MGGSELPR
ECCCCCCC
>Mature Secondary Structure 
GKITGFLEYERRHEAYEAPLTRVKHYKEFVAALTDEDAKIQGARCMDCGIPFCNNGCPV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWRQAIDVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCHHHHH
HAIIDKAWAQGWVAPQPAAHKTGKKVAVVGSGPAGLAAAQQLARAGHDVTVFEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGRDPLPESIGNMAKETISPETLKD
EEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHC
EFDAVVIAGGSETPRDLPVPGRELAGIHFAMEFLPQQNRVNAGDKVADQLLAKGKHVVVI
CCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEE
GGGDTGSDCVGTSNRHGAKHITQFELLPQPPEAENKPLVWPYWPIKLRTSSSHEEGCERD
ECCCCCCCCCCCCCCCCCHHCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCHHHCCHH
WAVATKRFEGKNGKVEKLIAVRVAWVDGKMQEVPNSEFEIKADLVLLAMGFTQPVAPVLE
HHHHHHHCCCCCCCEEEEEEEEEEEECCHHHHCCCCCEEEEEEEEEEEECCCCHHHHHHH
AFGVAKDARGNVRAGTEGDRAYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
HHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHEE
MGGSELPR
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]