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The map label for this gene is prfA [H]

Identifier: 83720975

GI number: 83720975

Start: 3368458

End: 3369540

Strand: Direct

Name: prfA [H]

Synonym: BTH_I2930

Alternate gene names: 83720975

Gene position: 3368458-3369540 (Clockwise)

Preceding gene: 161723170

Following gene: 83720020

Centisome position: 88.43

GC content: 66.85

Gene sequence:

>1083_bases
ATGAAGACGAGCATGCAAAGCAAGCTCGACCAGCTCACAACCCGGCTGGCCGAACTGAACGACCTGTTGAGCCGCGAGAA
CGTCACCGCGGACCTCGACCAGTATCGCAAGCTGACGCGCGAGCACGCGGAGATCGGCCCCGTCGTCGAGCATTATGCGC
AGTGGCGCCAGGCGCGCGCCGACGAACTCGCCGCGCAGGAACTGCTCGCCGACGCGTCGATGCGCGACTTCGCCGAGGAC
GAGCTGCGCGGCGCGCGCGAGCGGATGAGCCGCCTCGCGGCCGAACTGCAGACGATGCTGCTGCCGAAGGACCCGAACGA
CGAACGCAACATCTTCGTCGAAATTCGCGCGGGCACGGGCGGCGACGAATCGGCGCTGTTCGCGGGCGACCTGCTGCGGA
TGTACCTGCGCTACGCGGAGCGGCAGCGCTGGCAGGTCGAGATGATGTCGGAGAGCCCGTCGGATCTCGGCGGCTACAAG
GAAGTGATCGTGCGGATCGCGGGCTATGGCGCGTACTCGCGCCTGAAGTTCGAATCGGGCGGCCACCGCGTGCAGCGCGT
GCCCGCCACCGAGACGCAGGGCCGCATCCACACGTCCGCATGCACGGTCGCGGTGATGCCGGAGGCCGACGAGATCGGCG
AGGTCGAGATCAATCCGGCCGATCTGCGGATCGACACGTTCCGCGCGTCGGGCGCGGGCGGCCAGCACATCAACAAGACC
GATTCGGCGGTGCGCGTCACGCACATTCCGACAGGGATCGTCGTCGAGTGCCAGGACGACCGCTCGCAGCACAAGAACAA
GGACCGTGCGCTAAAGGTGCTCGCCGCGCGGATCAAGGACAAGCAATATCACGAGCAGCACGCGAAGGAAGCGGCCACGC
GCAAGAGCCTGATCGGCTCGGGCGACCGCTCCGAGCGGATACGCACGTACAACTTCCCGCAGGGCCGGATGACCGATCAC
CGGATCAACCTGACGCTGTACAAGCTCGAGCAGATCATGGACGGCGATCTCGACGAGCTGATCGCCGCGCTCGTCAGCGA
GCACCAGGCCGAGCTGCTCGCGTCGCTCGGCGACGCCGAATGA

Upstream 100 bases:

>100_bases
CGCCCCGCTCGTCGGACACGTCGGACCATTAGCGGCGCCCGGCCTGCCCGCCCCTTCCTTTCCCTTTTCGAATTCTTCCC
TGGGAGCATCGCTCCGCACC

Downstream 100 bases:

>100_bases
CGCCGCCCGCCCCACGCGCCCCCCGATGAACACGACGAGCCCCGCGCACGCCACCGCCGCCCGCCTGCTGCGCGCGTCGC
CGCTCGACGCGGTCGACGCG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKTSMQSKLDQLTTRLAELNDLLSRENVTADLDQYRKLTREHAEIGPVVEHYAQWRQARADELAAQELLADASMRDFAED
ELRGARERMSRLAAELQTMLLPKDPNDERNIFVEIRAGTGGDESALFAGDLLRMYLRYAERQRWQVEMMSESPSDLGGYK
EVIVRIAGYGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYKLEQIMDGDLDELIAALVSEHQAELLASLGDAE

Sequences:

>Translated_360_residues
MKTSMQSKLDQLTTRLAELNDLLSRENVTADLDQYRKLTREHAEIGPVVEHYAQWRQARADELAAQELLADASMRDFAED
ELRGARERMSRLAAELQTMLLPKDPNDERNIFVEIRAGTGGDESALFAGDLLRMYLRYAERQRWQVEMMSESPSDLGGYK
EVIVRIAGYGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYKLEQIMDGDLDELIAALVSEHQAELLASLGDAE
>Mature_360_residues
MKTSMQSKLDQLTTRLAELNDLLSRENVTADLDQYRKLTREHAEIGPVVEHYAQWRQARADELAAQELLADASMRDFAED
ELRGARERMSRLAAELQTMLLPKDPNDERNIFVEIRAGTGGDESALFAGDLLRMYLRYAERQRWQVEMMSESPSDLGGYK
EVIVRIAGYGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYKLEQIMDGDLDELIAALVSEHQAELLASLGDAE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=284, Percent_Identity=49.6478873239437, Blast_Score=282, Evalue=4e-76,
Organism=Homo sapiens, GI34577120, Length=371, Percent_Identity=38.0053908355795, Blast_Score=251, Evalue=5e-67,
Organism=Homo sapiens, GI166795305, Length=189, Percent_Identity=47.6190476190476, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=60.8333333333333, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI2367172, Length=334, Percent_Identity=38.9221556886228, Blast_Score=220, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=278, Percent_Identity=36.6906474820144, Blast_Score=182, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=353, Percent_Identity=40.2266288951841, Blast_Score=242, Evalue=6e-65,
Organism=Drosophila melanogaster, GI19921226, Length=268, Percent_Identity=44.7761194029851, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40529; Mature: 40529

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00850 GLY_RADICAL_1 ; PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSMQSKLDQLTTRLAELNDLLSRENVTADLDQYRKLTREHAEIGPVVEHYAQWRQARA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
DELAAQELLADASMRDFAEDELRGARERMSRLAAELQTMLLPKDPNDERNIFVEIRAGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GDESALFAGDLLRMYLRYAERQRWQVEMMSESPSDLGGYKEVIVRIAGYGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCCEEECEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRMTDHRINLTLYKLEQIMDGDLDELIAALVSEHQAELLASLGDAE
CCCCCCCEEECCCCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKTSMQSKLDQLTTRLAELNDLLSRENVTADLDQYRKLTREHAEIGPVVEHYAQWRQARA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
DELAAQELLADASMRDFAEDELRGARERMSRLAAELQTMLLPKDPNDERNIFVEIRAGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GDESALFAGDLLRMYLRYAERQRWQVEMMSESPSDLGGYKEVIVRIAGYGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCCEEECEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRMTDHRINLTLYKLEQIMDGDLDELIAALVSEHQAELLASLGDAE
CCCCCCCEEECCCCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA