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The map label for this gene is lpdA-1 [H]

Identifier: 83720959

GI number: 83720959

Start: 2911840

End: 2913270

Strand: Reverse

Name: lpdA-1 [H]

Synonym: BTH_I2554

Alternate gene names: 83720959

Gene position: 2913270-2911840 (Counterclockwise)

Preceding gene: 83720041

Following gene: 83721119

Centisome position: 76.48

GC content: 65.41

Gene sequence:

>1431_bases
ATGTCGAAGGAATTTGACGTCGTCGTGATCGGTGCGGGCCCGGGCGGCTATATCGCCGCGATCCGCGCCGCTCAACTGGG
CAAAACGGTTGCCTGCATCGAAAAATGGAAGAACGCGGCCGGCGCGCTGAAGCTGGGCGGCACGTGCCTGAACGTCGGCT
GCATCCCGTCGAAGGCGCTGCTCGCGTCGTCGGAGGAGTTCGAGAACGCGCAGCACCATCTCGCCGACCACGGCATCAGC
GTCGACGGCGTGAAGATGGACGTCGCGAAGATGCTCGCGCGCAAGGACGGGATCGTCGAGAAGATGACGGGCGGGATCGA
GTTCCTGTTCAAGAAGAACAAGATCACGTGGCTCAAGGGCCACGGCAAGTTCGCCGGCAAGACCGACGCCGGCGTGCAGA
TCGAGGTGAGCGGCGAGGGCGAGACGGAAGTCGTCACCGCGAAGAACGTGATCATCGCGACGGGCTCGAAGGCGCGTCAC
CTGCCGAACGTGCCGGTCGACAACAAGATCGTCGCGGACAACGAAGGCGCGCTGACGTTCGACTCGGTGCCGAAGAAGCT
CGCCGTGATCGGCGCGGGCGTGATCGGCCTCGAGCTCGGCTCGGTGTGGCGCCGTCTCGGCGCCGAAGTGACGGTGCTCG
AAGCGCTGCCGGCGTTCCTCGGCGCGGCCGACGAGGCGCTCGCGAAGGAAGCGGCGAAGCTGTTCAAGAAGCAGGGCCTC
GACATCCACCTCGGCGTGAAGATCGGCGAAGTGAAGACGAGCGCGAACGGCGTGTCGATCGCTTACACCGACAAGGACGG
CAACGCGAAGACGCTCGACGCCGACCGCCTGATCGTGTCGGTCGGCCGCGTGCCGAACACCGACAACCTCGGCCTCGAGG
CGATCGGCCTGAAGGTGAACGAGCGCGGCTTCATCGACGTCGACGACCACTGCCGCACCGCGGTGCCGAACGTGTACGCG
ATCGGCGATGTGGTGCGCGGCCCGATGCTCGCGCACAAGGCGGAAGACGAGGGCGTGCTGGTTGCGGAAGTGATCGACGG
CCAGAAGCCGCATATCGACTACAACTGCATTCCGTGGGTGATCTACACGTACCCGGAAATCGCGTGGGTCGGCAAGACGG
AGCAGCAGTTGAAGGCCGAGGGCCGCGAGATCAAGTCGGGCAAGTTCCCGTTCTCGATCAACGGCCGCGCGCTCGGCATG
AACGCGCCGGACGGCTTCGTGAAGATGATCGCGGATGCGAAGACCGACGAGCTGCTCGGCGTGCACGTGATCGGCGCGAA
CGCGTCGGACCTGATCGCCGAAGCCGTCGTCGCGATGGAGTTCAAGGCGGCGTCGGAAGACATCGCCCGCATCTGCCATC
CGCACCCGTCGATGTCCGAAGTGATGCGCGAAGCGGCGCTCGCCGTCGACAAGCGCTCGCTCAACAGCTAA

Upstream 100 bases:

>100_bases
AGCGCGCTTTTCACACCGTATCGAACCGCTCCGGCGGGCCGCGCGGGCGCCCATTGCGAGGCGCCTGCTCGGCTTACCGG
AGCCAAACCAAAGGATTGTC

Downstream 100 bases:

>100_bases
GCGCGACAACGCAGCACCGCGCGCCGGCCCTCGCGGCCGGCGTCGCAGTTTCTCGGCGAAGGCGGGCGGGGTTTCCCGCT
CGCCTTCGTTTTTGCCGGTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNAAGALKLGGTCLNVGCIPSKALLASSEEFENAQHHLADHGIS
VDGVKMDVAKMLARKDGIVEKMTGGIEFLFKKNKITWLKGHGKFAGKTDAGVQIEVSGEGETEVVTAKNVIIATGSKARH
LPNVPVDNKIVADNEGALTFDSVPKKLAVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEALAKEAAKLFKKQGL
DIHLGVKIGEVKTSANGVSIAYTDKDGNAKTLDADRLIVSVGRVPNTDNLGLEAIGLKVNERGFIDVDDHCRTAVPNVYA
IGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWVIYTYPEIAWVGKTEQQLKAEGREIKSGKFPFSINGRALGM
NAPDGFVKMIADAKTDELLGVHVIGANASDLIAEAVVAMEFKAASEDIARICHPHPSMSEVMREAALAVDKRSLNS

Sequences:

>Translated_476_residues
MSKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNAAGALKLGGTCLNVGCIPSKALLASSEEFENAQHHLADHGIS
VDGVKMDVAKMLARKDGIVEKMTGGIEFLFKKNKITWLKGHGKFAGKTDAGVQIEVSGEGETEVVTAKNVIIATGSKARH
LPNVPVDNKIVADNEGALTFDSVPKKLAVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEALAKEAAKLFKKQGL
DIHLGVKIGEVKTSANGVSIAYTDKDGNAKTLDADRLIVSVGRVPNTDNLGLEAIGLKVNERGFIDVDDHCRTAVPNVYA
IGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWVIYTYPEIAWVGKTEQQLKAEGREIKSGKFPFSINGRALGM
NAPDGFVKMIADAKTDELLGVHVIGANASDLIAEAVVAMEFKAASEDIARICHPHPSMSEVMREAALAVDKRSLNS
>Mature_475_residues
SKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNAAGALKLGGTCLNVGCIPSKALLASSEEFENAQHHLADHGISV
DGVKMDVAKMLARKDGIVEKMTGGIEFLFKKNKITWLKGHGKFAGKTDAGVQIEVSGEGETEVVTAKNVIIATGSKARHL
PNVPVDNKIVADNEGALTFDSVPKKLAVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEALAKEAAKLFKKQGLD
IHLGVKIGEVKTSANGVSIAYTDKDGNAKTLDADRLIVSVGRVPNTDNLGLEAIGLKVNERGFIDVDDHCRTAVPNVYAI
GDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWVIYTYPEIAWVGKTEQQLKAEGREIKSGKFPFSINGRALGMN
APDGFVKMIADAKTDELLGVHVIGANASDLIAEAVVAMEFKAASEDIARICHPHPSMSEVMREAALAVDKRSLNS

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=47.7516059957173, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI50301238, Length=470, Percent_Identity=28.0851063829787, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI33519430, Length=468, Percent_Identity=25, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519428, Length=468, Percent_Identity=25, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519426, Length=468, Percent_Identity=25, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI148277065, Length=468, Percent_Identity=25, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI148277071, Length=468, Percent_Identity=25, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI22035672, Length=474, Percent_Identity=27.0042194092827, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI291045266, Length=453, Percent_Identity=24.2825607064018, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI291045268, Length=448, Percent_Identity=22.9910714285714, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1786307, Length=468, Percent_Identity=39.1025641025641, Blast_Score=324, Evalue=9e-90,
Organism=Escherichia coli, GI87082354, Length=474, Percent_Identity=32.0675105485232, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1789915, Length=465, Percent_Identity=30.1075268817204, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI87081717, Length=474, Percent_Identity=29.1139240506329, Blast_Score=167, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI32565766, Length=471, Percent_Identity=49.4692144373673, Blast_Score=449, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17557007, Length=493, Percent_Identity=27.789046653144, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71983419, Length=465, Percent_Identity=28.3870967741935, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=464, Percent_Identity=28.2327586206897, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=487, Percent_Identity=25.0513347022587, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17559934, Length=193, Percent_Identity=30.5699481865285, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=46.0580912863071, Blast_Score=415, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=30.398322851153, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6325166, Length=472, Percent_Identity=27.5423728813559, Blast_Score=160, Evalue=5e-40,
Organism=Drosophila melanogaster, GI21358499, Length=470, Percent_Identity=50.2127659574468, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24640553, Length=490, Percent_Identity=27.7551020408163, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24640549, Length=490, Percent_Identity=27.7551020408163, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24640551, Length=490, Percent_Identity=27.7551020408163, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17737741, Length=487, Percent_Identity=25.4620123203285, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50421; Mature: 50289

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNAAGALKLGGTCLNVGCIPSKAL
CCCCCCEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHCCCCEEEECCEEEEECCCCCHHH
LASSEEFENAQHHLADHGISVDGVKMDVAKMLARKDGIVEKMTGGIEFLFKKNKITWLKG
HCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHCCCEEEEEECCEEEEEEC
HGKFAGKTDAGVQIEVSGEGETEVVTAKNVIIATGSKARHLPNVPVDNKIVADNEGALTF
CCCCCCCCCCCEEEEECCCCCEEEEEECEEEEECCCCCCCCCCCCCCCEEEECCCCCEEH
DSVPKKLAVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEALAKEAAKLFKKQGL
HHCCCEEHHHCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHCCC
DIHLGVKIGEVKTSANGVSIAYTDKDGNAKTLDADRLIVSVGRVPNTDNLGLEAIGLKVN
EEEEEEEEEEEEECCCCEEEEEECCCCCCEEECCCEEEEEECCCCCCCCCCEEEEEEEEC
ERGFIDVDDHCRTAVPNVYAIGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWV
CCCCEECCCCHHHCCCCEEEEHHHHCCCHHEECCCCCCEEEEEECCCCCCCCCCCCCCEE
IYTYPEIAWVGKTEQQLKAEGREIKSGKFPFSINGRALGMNAPDGFVKMIADAKTDELLG
EEECCCEEEECCCHHHHHHCCCCCCCCCCCEEECCEEEECCCCHHHHHHHHCCCCCCEEE
VHVIGANASDLIAEAVVAMEFKAASEDIARICHPHPSMSEVMREAALAVDKRSLNS
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNAAGALKLGGTCLNVGCIPSKAL
CCCCCEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHCCCCEEEECCEEEEECCCCCHHH
LASSEEFENAQHHLADHGISVDGVKMDVAKMLARKDGIVEKMTGGIEFLFKKNKITWLKG
HCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHCCCEEEEEECCEEEEEEC
HGKFAGKTDAGVQIEVSGEGETEVVTAKNVIIATGSKARHLPNVPVDNKIVADNEGALTF
CCCCCCCCCCCEEEEECCCCCEEEEEECEEEEECCCCCCCCCCCCCCCEEEECCCCCEEH
DSVPKKLAVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEALAKEAAKLFKKQGL
HHCCCEEHHHCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHCCC
DIHLGVKIGEVKTSANGVSIAYTDKDGNAKTLDADRLIVSVGRVPNTDNLGLEAIGLKVN
EEEEEEEEEEEEECCCCEEEEEECCCCCCEEECCCEEEEEECCCCCCCCCCEEEEEEEEC
ERGFIDVDDHCRTAVPNVYAIGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWV
CCCCEECCCCHHHCCCCEEEEHHHHCCCHHEECCCCCCEEEEEECCCCCCCCCCCCCCEE
IYTYPEIAWVGKTEQQLKAEGREIKSGKFPFSINGRALGMNAPDGFVKMIADAKTDELLG
EEECCCEEEECCCHHHHHHCCCCCCCCCCCEEECCEEEECCCCHHHHHHHHCCCCCCEEE
VHVIGANASDLIAEAVVAMEFKAASEDIARICHPHPSMSEVMREAALAVDKRSLNS
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]