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The map label for this gene is sucB [H]

Identifier: 83720041

GI number: 83720041

Start: 2913370

End: 2914647

Strand: Reverse

Name: sucB [H]

Synonym: BTH_I2555

Alternate gene names: 83720041

Gene position: 2914647-2913370 (Counterclockwise)

Preceding gene: 83719912

Following gene: 83720959

Centisome position: 76.52

GC content: 67.92

Gene sequence:

>1278_bases
ATGGCTATCGTAGAAGTCAAAGTCCCCCAGCTCTCCGAATCGGTTTCGGAAGCGACCATGCTGCAGTGGAAGAAGAAGCC
CGGCGAAGCCGTCGCGCAAGACGAAATCCTGATCGAGCTCGAGACCGACAAGGTCGTGCTCGAAGTGCCGGCGCCCGCCG
CGGGCGTGCTCGCGCAAGTGCTGCAGAACGATGGTGACACGGTCGTCGCCGACCAGGTGATCGCGACGATCGACACCGAG
GCGAAGGCAGGTGCGGCCGCAGCGGCAGCAGGCGCGGCCGAAGTCCAGCCGGCCGCCGCGCCCGCCGCCGCGCCGGCGCC
CGCCGCGCAGCCGGCCGCCGCCGCCGCGTCGAGCACCGCGTCCGCGTCGCCCGCCGCATCGAAGCTGATGGCCGAGAAGG
GCTTGGGCGCCGCCGACGTCGCCGGCTCGGGCCGCGACGGCCGCATCACGAAGGGCGACGTGCTGTCCGCGGGCAGCGCG
CCGAAGGCCGCACCGGCCGCCGCGCCGGCGAAGGCCGCGGCAAAGCCGTCGCTGCCGGACGTGAAGGTGCCGGCGTCGGC
GACGACGTGGCTCAACGACCGTCCGGAGCAGCGCGTGCCGATGTCGCGCCTGCGCGCGCGCATTGCCGAGCGTCTGCTCG
AATCGCAGCAGACCAACGCGATCCTCACGACGTTCAACGAAGTGAACATGCAGCCGGTGATGGACCTGCGCGCGAAGTAC
AAGGACAAGTTCGAGAAGGAACACGGCGTGAAGCTCGGCTTCATGTCGTTCTTCGTGAAGGCGGCCGTTCACGCGCTGAA
GAAGTTCCCGCTCGTGAACGCGTCGATCGACGGCAACGACATCGTCTACCACGGCTACTTCGACATCGGCATCGCGGTCG
GTTCGCCGCGCGGCCTCGTCGTGCCGATCCTGCGCAACGCGGATCAGCTGAGCCTCGCCGAGATCGAGAAGAAGATCGCC
GAATTCGGCCAGAAGGCGAAGGACGGCAAGCTGTCGATCGAGGAAATGACGGGCGGCACGTTCTCGATCTCGAACGGCGG
CGTGTTCGGCTCGATGCTGTCGACGCCGATCATCAACCCGCCGCAGTCGGCCATCCTCGGCGTGCACGCGACGAAGGAGC
GCCCGGTCGTGGAGAACGGCCAGATCGTGATCCGTCCGATCAACTACCTCGCGCTGTCGTACGACCACCGGATCATCGAC
GGCCGTGAAGCGGTGCTGTCGCTCGTCGCGATGAAGGACGCGCTCGAAGATCCGGCGCGCCTGCTGCTCGACCTGTAA

Upstream 100 bases:

>100_bases
TTTCCCGCGCGCTTTCGAACGCAGGCGCCGCGCGCCGCGATATTCGACAGCGCACGCGCACGCCTCGTCGTTACCCAGAT
TAAGCATCCAGGAAATTCAC

Downstream 100 bases:

>100_bases
GCGCGCTTTTCACACCGTATCGAACCGCTCCGGCGGGCCGCGCGGGCGCCCATTGCGAGGCGCCTGCTCGGCTTACCGGA
GCCAAACCAAAGGATTGTCA

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQVLQNDGDTVVADQVIATIDTE
AKAGAAAAAAGAAEVQPAAAPAAAPAPAAQPAAAAASSTASASPAASKLMAEKGLGAADVAGSGRDGRITKGDVLSAGSA
PKAAPAAAPAKAAAKPSLPDVKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDLRAKY
KDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIEKKIA
EFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIID
GREAVLSLVAMKDALEDPARLLLDL

Sequences:

>Translated_425_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQVLQNDGDTVVADQVIATIDTE
AKAGAAAAAAGAAEVQPAAAPAAAPAPAAQPAAAAASSTASASPAASKLMAEKGLGAADVAGSGRDGRITKGDVLSAGSA
PKAAPAAAPAKAAAKPSLPDVKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDLRAKY
KDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIEKKIA
EFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIID
GREAVLSLVAMKDALEDPARLLLDL
>Mature_424_residues
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQVLQNDGDTVVADQVIATIDTEA
KAGAAAAAAGAAEVQPAAAPAAAPAPAAQPAAAAASSTASASPAASKLMAEKGLGAADVAGSGRDGRITKGDVLSAGSAP
KAAPAAAPAKAAAKPSLPDVKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDLRAKYK
DKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIEKKIAE
FGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDG
REAVLSLVAMKDALEDPARLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=234, Percent_Identity=56.4102564102564, Blast_Score=285, Evalue=4e-77,
Organism=Homo sapiens, GI31711992, Length=445, Percent_Identity=31.4606741573034, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI110671329, Length=437, Percent_Identity=28.1464530892449, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI203098753, Length=467, Percent_Identity=29.3361884368308, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI203098816, Length=467, Percent_Identity=29.3361884368308, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.888198757764, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1786946, Length=425, Percent_Identity=52.7058823529412, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1786305, Length=430, Percent_Identity=33.2558139534884, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=438, Percent_Identity=42.4657534246575, Blast_Score=329, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17560088, Length=451, Percent_Identity=32.3725055432373, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=449, Percent_Identity=27.3942093541203, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17538894, Length=325, Percent_Identity=32, Blast_Score=144, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6320352, Length=418, Percent_Identity=44.0191387559809, Blast_Score=334, Evalue=1e-92,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=29.9559471365639, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24645909, Length=241, Percent_Identity=55.6016597510373, Blast_Score=282, Evalue=2e-76,
Organism=Drosophila melanogaster, GI18859875, Length=441, Percent_Identity=28.7981859410431, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=34.6491228070175, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=34.6491228070175, Blast_Score=132, Evalue=5e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44332; Mature: 44201

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQV
CEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHH
LQNDGDTVVADQVIATIDTEAKAGAAAAAAGAAEVQPAAAPAAAPAPAAQPAAAAASSTA
HHCCCCEEEHHHHHHEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCC
SASPAASKLMAEKGLGAADVAGSGRDGRITKGDVLSAGSAPKAAPAAAPAKAAAKPSLPD
CCCHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHCCCCCCCC
VKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDLRAKY
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCHHHHHHHHH
KDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLV
HHHHHHHHCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEE
VPILRNADQLSLAEIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINP
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCCCCCCC
PQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGREAVLSLVAMKDALEDPAR
CHHHEEEEECCCCCCCCCCCCEEEEECCEEEEECCCEEECCHHHHHHHHHHHHHHCCHHH
LLLDL
HHHCC
>Mature Secondary Structure 
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQV
EEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHH
LQNDGDTVVADQVIATIDTEAKAGAAAAAAGAAEVQPAAAPAAAPAPAAQPAAAAASSTA
HHCCCCEEEHHHHHHEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCC
SASPAASKLMAEKGLGAADVAGSGRDGRITKGDVLSAGSAPKAAPAAAPAKAAAKPSLPD
CCCHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHCCCCCCCC
VKVPASATTWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMQPVMDLRAKY
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCHHHHHHHHH
KDKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLV
HHHHHHHHCCEEHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEE
VPILRNADQLSLAEIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINP
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCCCCCCC
PQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGREAVLSLVAMKDALEDPAR
CHHHEEEEECCCCCCCCCCCCEEEEECCEEEEECCCEEECCHHHHHHHHHHHHHHCCHHH
LLLDL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]