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The map label for this gene is leuA-1 [H]

Identifier: 83720826

GI number: 83720826

Start: 510108

End: 511748

Strand: Reverse

Name: leuA-1 [H]

Synonym: BTH_I0459

Alternate gene names: 83720826

Gene position: 511748-510108 (Counterclockwise)

Preceding gene: 83720132

Following gene: 83718896

Centisome position: 13.43

GC content: 68.98

Gene sequence:

>1641_bases
ATGAAGCGCAACCCCGCCGAGAAATACCGACCGTTCGAACCCGTTCGCATCAACGGACGCCGATGGCCGAGCCGCACGAT
CGAGCGCGCGCCGATCTGGATGAGCACCGATCTGCGCGACGGCAACCAGTCGTTGATCGAGCCGATGACGATCGAGCAGA
AGCTCGAATTCTTCGAGATGCTCGTCGCGATCGGCTTCAAGGAAATCGAAGTCGGTTTTCCGTCGGCGTCGCAAACCGAC
TTCGATTTCGTGCGCAAGCTGATCGTCGAGAAGCGCATCCCCGACGACGTGACGATCGAAGTGCTCGTGCAGTCGCGCGA
AGACCTGATCGCGCACACGTTCGACGCGCTCGAAGGCGCGCCGCGCGCGATCGTCCATCTGTACAACGCGATCTGCCCGT
CGTTCCGCCGGATCGTGTTCGGGATGTCGAAGGACGACGTGAAGGCGCTCGCCGTCGACGGCACGCGAATCATCAAGGAG
CACGCGCAGGCGCGCCCCGGCACGCACTGGACGTTCCAGTACTCGCCCGAGACGTTCAGCATGACCGAGCTGCCGTTCGC
GCGCGAAGTCTGCGACGCGGTCGCGCAGACGTGGCGGCCGACGCGCGATCACAAGATGATCGTGAACCTGCCCGCCACCG
TCGAGGCCGCGACGCCGAACGTGTTCGCCGACCAGATCGAATGGATGGACCGCAACCTCGGCTACCGCGACAGCATCGTG
CTGTCGGTGCACCCGCACAACGATCGCGGCACCGCGGTCGCGGCGGCCGAGCTGGCGCTGCTCGCGGGCGCGGACCGCAT
CGAAGGATGCCTGTTCGGCAACGGCGAGCGCACGGGCAACGTCGATCTCGTCACGCTCGCGCTGAACCTGTACACGCAAG
GGATCGATCCGGGCCTCGATTTCTCCGACATCGACGCGGTGCGCCGCGTCGTCGAACGCTGCAACCGGATTCCCGTGCAT
CCGCGCCATCCGTACGCGGGCGATCTCGTGTTCACCGCGTTCTCCGGCTCGCATCAGGACGCGATCCGCAAGGGCCTCGC
GCAGCAGCGGCCCGACGCGATCTGGGAGGTGCCGTACCTGCCGATCGACCCCGCCGACCTCGGCCGCAGCTACGACGCCG
TGATCCGCGTGAACAGCCAGTCCGGCAAGGGCGGCGCGACGTACCTGCTCGAGCGCGGGATGGGCTTCTCGCCGCCGCGT
CGCGTGCAGATCGAATTCAGCCACGCGGTGCAGGCGCTCGCCGACGCGTCCGGCGAGGAAGTGACGGCAGACGCCATTTG
CGCGCTCTTCACGCGCGAATATCTGCAGACGATGGGCCCGGCCGCGCGCGCGGGCGCGTCGGCGAGCTGGCATGACCGCG
AAGTCGCGCTCGCGCACGCCGAAGACGCGCCGAGCGCGGCGCGGCAGGCCGCGGCCGCATTCGCCGCGGCGGCGGGCGTC
GCGATCGACGTCACGTCGTGCGAGGTCGAGCGCACCGCGAACGGCGACACCGTCGTGTTCGTCGGCGCGAAAGTCGGCGA
CGCCGCGCTGCGCCACGGCGCGGGCGTGCATCGCGATCCGGCCGCGGCCGCGACGGACGCGGTCGCGAGCGCGATCAACC
GCTCGGCCTGGAGCCGCGACAAGCGGCGCGCCGCGGCCTGA

Upstream 100 bases:

>100_bases
TCCGCGCCGTCGTGCCATCGGGCATGCACGCGCGCAGGCAGCGCCCACCGGCGCGTCATGCCGTCCGTCTTCGCTCGCGA
CTTGACCGCCCGAGGCAAAG

Downstream 100 bases:

>100_bases
CGCGGCGAATTCGACGACAGACGCAAGACACACGCGCGGCGCACGCCGCCGCGCAACGGCCGGTGCGACGCGCGATCCGC
GGCGCGCCGGCGAGAAACCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MKRNPAEKYRPFEPVRINGRRWPSRTIERAPIWMSTDLRDGNQSLIEPMTIEQKLEFFEMLVAIGFKEIEVGFPSASQTD
FDFVRKLIVEKRIPDDVTIEVLVQSREDLIAHTFDALEGAPRAIVHLYNAICPSFRRIVFGMSKDDVKALAVDGTRIIKE
HAQARPGTHWTFQYSPETFSMTELPFAREVCDAVAQTWRPTRDHKMIVNLPATVEAATPNVFADQIEWMDRNLGYRDSIV
LSVHPHNDRGTAVAAAELALLAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLDFSDIDAVRRVVERCNRIPVH
PRHPYAGDLVFTAFSGSHQDAIRKGLAQQRPDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGATYLLERGMGFSPPR
RVQIEFSHAVQALADASGEEVTADAICALFTREYLQTMGPAARAGASASWHDREVALAHAEDAPSAARQAAAAFAAAAGV
AIDVTSCEVERTANGDTVVFVGAKVGDAALRHGAGVHRDPAAAATDAVASAINRSAWSRDKRRAAA

Sequences:

>Translated_546_residues
MKRNPAEKYRPFEPVRINGRRWPSRTIERAPIWMSTDLRDGNQSLIEPMTIEQKLEFFEMLVAIGFKEIEVGFPSASQTD
FDFVRKLIVEKRIPDDVTIEVLVQSREDLIAHTFDALEGAPRAIVHLYNAICPSFRRIVFGMSKDDVKALAVDGTRIIKE
HAQARPGTHWTFQYSPETFSMTELPFAREVCDAVAQTWRPTRDHKMIVNLPATVEAATPNVFADQIEWMDRNLGYRDSIV
LSVHPHNDRGTAVAAAELALLAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLDFSDIDAVRRVVERCNRIPVH
PRHPYAGDLVFTAFSGSHQDAIRKGLAQQRPDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGATYLLERGMGFSPPR
RVQIEFSHAVQALADASGEEVTADAICALFTREYLQTMGPAARAGASASWHDREVALAHAEDAPSAARQAAAAFAAAAGV
AIDVTSCEVERTANGDTVVFVGAKVGDAALRHGAGVHRDPAAAATDAVASAINRSAWSRDKRRAAA
>Mature_546_residues
MKRNPAEKYRPFEPVRINGRRWPSRTIERAPIWMSTDLRDGNQSLIEPMTIEQKLEFFEMLVAIGFKEIEVGFPSASQTD
FDFVRKLIVEKRIPDDVTIEVLVQSREDLIAHTFDALEGAPRAIVHLYNAICPSFRRIVFGMSKDDVKALAVDGTRIIKE
HAQARPGTHWTFQYSPETFSMTELPFAREVCDAVAQTWRPTRDHKMIVNLPATVEAATPNVFADQIEWMDRNLGYRDSIV
LSVHPHNDRGTAVAAAELALLAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLDFSDIDAVRRVVERCNRIPVH
PRHPYAGDLVFTAFSGSHQDAIRKGLAQQRPDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGATYLLERGMGFSPPR
RVQIEFSHAVQALADASGEEVTADAICALFTREYLQTMGPAARAGASASWHDREVALAHAEDAPSAARQAAAAFAAAAGV
AIDVTSCEVERTANGDTVVFVGAKVGDAALRHGAGVHRDPAAAATDAVASAINRSAWSRDKRRAAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=392, Percent_Identity=25.2551020408163, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324225, Length=442, Percent_Identity=55.6561085972851, Blast_Score=525, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6324682, Length=442, Percent_Identity=54.2986425339367, Blast_Score=517, Evalue=1e-147,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 59771; Mature: 59771

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNPAEKYRPFEPVRINGRRWPSRTIERAPIWMSTDLRDGNQSLIEPMTIEQKLEFFEM
CCCCCCHHCCCCCCEEECCCCCCCCHHCCCCEEEECCCCCCCHHHHCCHHHHHHHHHHHH
LVAIGFKEIEVGFPSASQTDFDFVRKLIVEKRIPDDVTIEVLVQSREDLIAHTFDALEGA
HHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCC
PRAIVHLYNAICPSFRRIVFGMSKDDVKALAVDGTRIIKEHAQARPGTHWTFQYSPETFS
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCC
MTELPFAREVCDAVAQTWRPTRDHKMIVNLPATVEAATPNVFADQIEWMDRNLGYRDSIV
CCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHCCCCCCCEEE
LSVHPHNDRGTAVAAAELALLAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLD
EEEECCCCCCCHHHHHHHHHHHCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCCCC
FSDIDAVRRVVERCNRIPVHPRHPYAGDLVFTAFSGSHQDAIRKGLAQQRPDAIWEVPYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEECCCC
PIDPADLGRSYDAVIRVNSQSGKGGATYLLERGMGFSPPRRVQIEFSHAVQALADASGEE
CCCHHHHCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCEEEEEHHHHHHHHHCCCCCC
VTADAICALFTREYLQTMGPAARAGASASWHDREVALAHAEDAPSAARQAAAAFAAAAGV
CHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCC
AIDVTSCEVERTANGDTVVFVGAKVGDAALRHGAGVHRDPAAAATDAVASAINRSAWSRD
EEEEECCEEEECCCCCEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KRRAAA
HHHCCC
>Mature Secondary Structure
MKRNPAEKYRPFEPVRINGRRWPSRTIERAPIWMSTDLRDGNQSLIEPMTIEQKLEFFEM
CCCCCCHHCCCCCCEEECCCCCCCCHHCCCCEEEECCCCCCCHHHHCCHHHHHHHHHHHH
LVAIGFKEIEVGFPSASQTDFDFVRKLIVEKRIPDDVTIEVLVQSREDLIAHTFDALEGA
HHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCC
PRAIVHLYNAICPSFRRIVFGMSKDDVKALAVDGTRIIKEHAQARPGTHWTFQYSPETFS
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCC
MTELPFAREVCDAVAQTWRPTRDHKMIVNLPATVEAATPNVFADQIEWMDRNLGYRDSIV
CCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCHHHHHHHHHHCCCCCCCEEE
LSVHPHNDRGTAVAAAELALLAGADRIEGCLFGNGERTGNVDLVTLALNLYTQGIDPGLD
EEEECCCCCCCHHHHHHHHHHHCCHHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCCCC
FSDIDAVRRVVERCNRIPVHPRHPYAGDLVFTAFSGSHQDAIRKGLAQQRPDAIWEVPYL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEECCCC
PIDPADLGRSYDAVIRVNSQSGKGGATYLLERGMGFSPPRRVQIEFSHAVQALADASGEE
CCCHHHHCCCEEEEEEEECCCCCCCHHHHHHCCCCCCCCCEEEEEHHHHHHHHHCCCCCC
VTADAICALFTREYLQTMGPAARAGASASWHDREVALAHAEDAPSAARQAAAAFAAAAGV
CHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCC
AIDVTSCEVERTANGDTVVFVGAKVGDAALRHGAGVHRDPAAAATDAVASAINRSAWSRD
EEEEECCEEEECCCCCEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KRRAAA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA