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The map label for this gene is dmlR [H]

Identifier: 83720817

GI number: 83720817

Start: 2449760

End: 2450671

Strand: Direct

Name: dmlR [H]

Synonym: BTH_I2174

Alternate gene names: 83720817

Gene position: 2449760-2450671 (Clockwise)

Preceding gene: 83720784

Following gene: 83721231

Centisome position: 64.31

GC content: 70.29

Gene sequence:

>912_bases
ATGGACCGATTCAAGCAGATCGAAACCTTCGTGCGGGTGGCCGACGCGGGCAGCCTCGCGGCGGCCGCGCTCGAGGAGGG
CGTGTCGCCCGTCGTGCTCGGGCGGCGGATCGACGCGCTCGAGAAGCGCCTCGGCGTGAAGCTGATGTACCGGTCGACGC
GCCGGCTCGTCGTGAGCGAGGACGGCGCCGCGTTCCTCGAGCGCTGTCGCGGGCTCCTCACCGAGTGGGCGCAGGCCGAG
AACGAGGTTGTCGCGGGGCGCCGCGCGGTGGGCGGGCATCTGATCGTGTCGGCGCCCGCCGCGTTCGGCCGCAAGCACGT
CGCGCCGCTCGCGCCCGCGTTCATCGCCGACAAGCCCGACCTGCAGATGTCGTTCAACCTGACCGACCGCGTCGTCGATC
TCGTGCGCGAGGGCTACGATCTGTCGATCCGGATCGGCGGCGCGGTCGATCCGAACTTCGTCGCGGTGAAGCTCGCGTCG
AACCGGCGCGTCGTGTGCGGCACGCCCGAGTATTTTCGCCGGCACGGCCGGCCGAAGACGCTCGACGATCTCGCGGGCCA
CAACTGTCTCGCGTTCAATCTGCAGGGCGGCCAGAACCGCGGCTGGTACTTCCGGCGCAGCGGCAAGCTCGTGACGATGC
GCGTGTCGGGCACGCTCGACTGCAACGACGGCGAGCTCCTGCACCGCTGGGTGTCGGAGGGGCTCGGGCTCGGCTGGCGC
TCGACGTGGGAGATCGCGCAGCAGCTCGCGCGCGGCGAGCTCGAGACGGTGCTCGACGAGTACGCGCTGCCCGACTACGA
CATCCTCGCCGTCTATCCGCAGCAGCGCTACGTGCCGGCGCGCGTGCGCTACTTCATCGATTACTTGCGCGACGTCTATG
CGCGGCCGGGGTATTGGGAAGGCGCCGAGTGA

Upstream 100 bases:

>100_bases
GCAGGAAAGGTTTGATAAGATCGAGCCGAGTCGCTTATTTCGAGACTAAAAGTATCGAATGCCGGGCGCGCGCGACGGCG
CCGGGTGGGGGAGGCAGCGG

Downstream 100 bases:

>100_bases
GCGCGATCCGGCGCCGCGCGCGGTTCTCGAACGGGCGCCGGATCGCGAGGTCGCCGGATCGGGCGGGTCAGGCGGCCGCC
CTTCCCGCTACCCGACGACG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSEDGAAFLERCRGLLTEWAQAE
NEVVAGRRAVGGHLIVSAPAAFGRKHVAPLAPAFIADKPDLQMSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAS
NRRVVCGTPEYFRRHGRPKTLDDLAGHNCLAFNLQGGQNRGWYFRRSGKLVTMRVSGTLDCNDGELLHRWVSEGLGLGWR
STWEIAQQLARGELETVLDEYALPDYDILAVYPQQRYVPARVRYFIDYLRDVYARPGYWEGAE

Sequences:

>Translated_303_residues
MDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSEDGAAFLERCRGLLTEWAQAE
NEVVAGRRAVGGHLIVSAPAAFGRKHVAPLAPAFIADKPDLQMSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAS
NRRVVCGTPEYFRRHGRPKTLDDLAGHNCLAFNLQGGQNRGWYFRRSGKLVTMRVSGTLDCNDGELLHRWVSEGLGLGWR
STWEIAQQLARGELETVLDEYALPDYDILAVYPQQRYVPARVRYFIDYLRDVYARPGYWEGAE
>Mature_303_residues
MDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSEDGAAFLERCRGLLTEWAQAE
NEVVAGRRAVGGHLIVSAPAAFGRKHVAPLAPAFIADKPDLQMSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLAS
NRRVVCGTPEYFRRHGRPKTLDDLAGHNCLAFNLQGGQNRGWYFRRSGKLVTMRVSGTLDCNDGELLHRWVSEGLGLGWR
STWEIAQQLARGELETVLDEYALPDYDILAVYPQQRYVPARVRYFIDYLRDVYARPGYWEGAE

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=36.7697594501718, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=31.5789473684211, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=31.864406779661, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=29.3286219081272, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=26.2626262626263, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=27.027027027027, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=25.5244755244755, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33942; Mature: 33942

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSE
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHCEEEEEECCCEEEEEC
DGAAFLERCRGLLTEWAQAENEVVAGRRAVGGHLIVSAPAAFGRKHVAPLAPAFIADKPD
CCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEECCHHHCCCCCCCCCHHHHCCCCC
LQMSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLASNRRVVCGTPEYFRRHGRPKT
CEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEECCHHHHHHCCCCCC
LDDLAGHNCLAFNLQGGQNRGWYFRRSGKLVTMRVSGTLDCNDGELLHRWVSEGLGLGWR
HHHHCCCCEEEEEECCCCCCCEEEEECCCEEEEEECCEEECCCHHHHHHHHHHHCCCCCC
STWEIAQQLARGELETVLDEYALPDYDILAVYPQQRYVPARVRYFIDYLRDVYARPGYWE
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GAE
CCC
>Mature Secondary Structure
MDRFKQIETFVRVADAGSLAAAALEEGVSPVVLGRRIDALEKRLGVKLMYRSTRRLVVSE
CCHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHCEEEEEECCCEEEEEC
DGAAFLERCRGLLTEWAQAENEVVAGRRAVGGHLIVSAPAAFGRKHVAPLAPAFIADKPD
CCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEECCHHHCCCCCCCCCHHHHCCCCC
LQMSFNLTDRVVDLVREGYDLSIRIGGAVDPNFVAVKLASNRRVVCGTPEYFRRHGRPKT
CEEEECHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEECCHHHHHHCCCCCC
LDDLAGHNCLAFNLQGGQNRGWYFRRSGKLVTMRVSGTLDCNDGELLHRWVSEGLGLGWR
HHHHCCCCEEEEEECCCCCCCEEEEECCCEEEEEECCEEECCCHHHHHHHHHHHCCCCCC
STWEIAQQLARGELETVLDEYALPDYDILAVYPQQRYVPARVRYFIDYLRDVYARPGYWE
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GAE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]