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The map label for this gene is ansA [H]

Identifier: 83720784

GI number: 83720784

Start: 2443615

End: 2444658

Strand: Direct

Name: ansA [H]

Synonym: BTH_I2166

Alternate gene names: 83720784

Gene position: 2443615-2444658 (Clockwise)

Preceding gene: 83720116

Following gene: 83720817

Centisome position: 64.15

GC content: 71.36

Gene sequence:

>1044_bases
ATGAACGCACCGACATCTTCCCATCCCCCTCATGTGACGAACCCGGCGCCGCGCGCGCTGCCGCGCATCGCCGTCCTTGC
GACGGGCGGCACGATCGCGGGCGCCGCGCCCGACGCCGCGCAGACGGCCGGCTATCACGCGGGCGCGCTCGGCGTCGACA
GGCTGCTCGCCGCCGTGCCCGCGCTCGCGCAGATCGCGACGATCGAGGCCGAGCAGATTGCGAGCATCGACAGCAAGGAC
CTGTCCGTCGCGCTGTGGACGACGCTCGTCGAGCGGATCAACGCGCTTGCCGCGAGCGAGGCGATCGACGGCATCGTGAT
CACGCACGGCACCGACACGCTCGAAGAAACCGCGTACCTGCTGCACCTGACCGTGAAGACCGCGAAGCCCGTCGTGATGA
CGGCGGCGATGCGCCCGGCGACCGCGCTGTCGTCCGACGGCCCGCTCAACCTGCTGAACGCGGTGGCGGTCGCGGGCAGC
GCGGCGGCGCGCGGGCAGGGCGTGCTCGTCGCGTTCAACAACCGGATCCACAGCGCGCGCGACGTCGTGAAGACGAGCAC
CTACGCGGTCGACGCGTTTCATTCGCCGGAGCTCGGCGCGCTCGGCTGGGTGCAGGACGGCCGCATCGAGTTCGCGCGCC
GCGCGACGCGTGCGCATACGCTCGACACGCGCTTCGCGATCGGCGCGGCCTGGCCGCAGGTCGACGTCGTCACGAGCTAT
GCGGGCGCGTCGCGCGTGATCGTCGACGCGTGCGTCGCGGCCGGCGCGCGCGGGCTTGTCGTCGCAGGCACGGGCAACGG
TTCGATCCACGCGGCGGTGCAGACGGCGCTCGCCGATGCGGCGGCGAAAGGCGTTGCGATCGTGCGCGCGTCGCGTGTCG
GCTCGGGGCATGTGATGCGCAATGGCGCGGCGAACGACGATGCGCTCGGCTTCGTGAGCGCAGGCTCGCTCAATCCGTAC
AAGGCGCGCGTGCTGCTGATGCTCGCGCTCGCGGCGGGCATGACCGACGTGCACGAATTGCAGCAGGCGTTCGATACGTA
TTGA

Upstream 100 bases:

>100_bases
GCAACCGGTCGCGGCGCGGGGGCCGCGTCGCGCGGCCGCGCGCTCGGGAAAACTCCGCCCCCTTGCCGAAGGGTGGCCGG
CTGCCGTTACAATCGCGGCC

Downstream 100 bases:

>100_bases
TGATCGGACCGGCGTGCGCCGCCGTCGAGCGCGGCGCGCGGCCCGTCGATCGGGTGGGGCGCGCGCCGCCGGCGCGCGGA
TCGAGCCGCGTCGGATCGAA

Product: asparaginase family protein

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MNAPTSSHPPHVTNPAPRALPRIAVLATGGTIAGAAPDAAQTAGYHAGALGVDRLLAAVPALAQIATIEAEQIASIDSKD
LSVALWTTLVERINALAASEAIDGIVITHGTDTLEETAYLLHLTVKTAKPVVMTAAMRPATALSSDGPLNLLNAVAVAGS
AAARGQGVLVAFNNRIHSARDVVKTSTYAVDAFHSPELGALGWVQDGRIEFARRATRAHTLDTRFAIGAAWPQVDVVTSY
AGASRVIVDACVAAGARGLVVAGTGNGSIHAAVQTALADAAAKGVAIVRASRVGSGHVMRNGAANDDALGFVSAGSLNPY
KARVLLMLALAAGMTDVHELQQAFDTY

Sequences:

>Translated_347_residues
MNAPTSSHPPHVTNPAPRALPRIAVLATGGTIAGAAPDAAQTAGYHAGALGVDRLLAAVPALAQIATIEAEQIASIDSKD
LSVALWTTLVERINALAASEAIDGIVITHGTDTLEETAYLLHLTVKTAKPVVMTAAMRPATALSSDGPLNLLNAVAVAGS
AAARGQGVLVAFNNRIHSARDVVKTSTYAVDAFHSPELGALGWVQDGRIEFARRATRAHTLDTRFAIGAAWPQVDVVTSY
AGASRVIVDACVAAGARGLVVAGTGNGSIHAAVQTALADAAAKGVAIVRASRVGSGHVMRNGAANDDALGFVSAGSLNPY
KARVLLMLALAAGMTDVHELQQAFDTY
>Mature_347_residues
MNAPTSSHPPHVTNPAPRALPRIAVLATGGTIAGAAPDAAQTAGYHAGALGVDRLLAAVPALAQIATIEAEQIASIDSKD
LSVALWTTLVERINALAASEAIDGIVITHGTDTLEETAYLLHLTVKTAKPVVMTAAMRPATALSSDGPLNLLNAVAVAGS
AAARGQGVLVAFNNRIHSARDVVKTSTYAVDAFHSPELGALGWVQDGRIEFARRATRAHTLDTRFAIGAAWPQVDVVTSY
AGASRVIVDACVAAGARGLVVAGTGNGSIHAAVQTALADAAAKGVAIVRASRVGSGHVMRNGAANDDALGFVSAGSLNPY
KARVLLMLALAAGMTDVHELQQAFDTY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=227, Percent_Identity=29.0748898678414, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1789327, Length=331, Percent_Identity=47.1299093655589, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1788065, Length=295, Percent_Identity=30.8474576271186, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17558278, Length=330, Percent_Identity=27.5757575757576, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17558280, Length=330, Percent_Identity=27.5757575757576, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI193207374, Length=330, Percent_Identity=27.5757575757576, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI193207372, Length=330, Percent_Identity=27.5757575757576, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6320527, Length=338, Percent_Identity=34.6153846153846, Blast_Score=193, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6323189, Length=324, Percent_Identity=37.3456790123457, Blast_Score=192, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6323187, Length=324, Percent_Identity=37.3456790123457, Blast_Score=192, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6323186, Length=324, Percent_Identity=37.3456790123457, Blast_Score=192, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6323184, Length=324, Percent_Identity=37.3456790123457, Blast_Score=192, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24639609, Length=254, Percent_Identity=26.7716535433071, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24645530, Length=228, Percent_Identity=28.5087719298246, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35392; Mature: 35392

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPTSSHPPHVTNPAPRALPRIAVLATGGTIAGAAPDAAQTAGYHAGALGVDRLLAAVP
CCCCCCCCCCCCCCCCCHHCCEEEEEEECCEECCCCCCHHHHCCCCCCHHHHHHHHHHHH
ALAQIATIEAEQIASIDSKDLSVALWTTLVERINALAASEAIDGIVITHGTDTLEETAYL
HHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHCEEE
LHLTVKTAKPVVMTAAMRPATALSSDGPLNLLNAVAVAGSAAARGQGVLVAFNNRIHSAR
EEEEECCCCCEEEEECCCCCHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHH
DVVKTSTYAVDAFHSPELGALGWVQDGRIEFARRATRAHTLDTRFAIGAAWPQVDVVTSY
HHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHECC
AGASRVIVDACVAAGARGLVVAGTGNGSIHAAVQTALADAAAKGVAIVRASRVGSGHVMR
CCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCEEE
NGAANDDALGFVSAGSLNPYKARVLLMLALAAGMTDVHELQQAFDTY
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNAPTSSHPPHVTNPAPRALPRIAVLATGGTIAGAAPDAAQTAGYHAGALGVDRLLAAVP
CCCCCCCCCCCCCCCCCHHCCEEEEEEECCEECCCCCCHHHHCCCCCCHHHHHHHHHHHH
ALAQIATIEAEQIASIDSKDLSVALWTTLVERINALAASEAIDGIVITHGTDTLEETAYL
HHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHCEEE
LHLTVKTAKPVVMTAAMRPATALSSDGPLNLLNAVAVAGSAAARGQGVLVAFNNRIHSAR
EEEEECCCCCEEEEECCCCCHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHH
DVVKTSTYAVDAFHSPELGALGWVQDGRIEFARRATRAHTLDTRFAIGAAWPQVDVVTSY
HHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHECC
AGASRVIVDACVAAGARGLVVAGTGNGSIHAAVQTALADAAAKGVAIVRASRVGSGHVMR
CCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCEEE
NGAANDDALGFVSAGSLNPYKARVLLMLALAAGMTDVHELQQAFDTY
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8898907; 14500908 [H]