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The map label for this gene is aceF [H]

Identifier: 83720698

GI number: 83720698

Start: 2109378

End: 2111024

Strand: Direct

Name: aceF [H]

Synonym: BTH_I1865

Alternate gene names: 83720698

Gene position: 2109378-2111024 (Clockwise)

Preceding gene: 83718787

Following gene: 83720184

Centisome position: 55.38

GC content: 68.97

Gene sequence:

>1647_bases
ATGAGTCAAGCGATCGAAGTCAAGGTGCCGGATATCGGCGATTACAAGGACGTGCCCGTCATCGAGGTGCTCGTGAAGCC
GGGCGATGCGGTCGAGCCCGAGCAGTCGCTCGTCACGCTCGAGTCCGACAAGGCGACGATGGACGTGCCGAGCCCGTCGG
CGGGCACGGTGAAGGAAGTGAAGGTGAAGGTCGGCGACGCGGTGTCGCAAGGTTCGCTGATCGTGCTGCTCGACGGCGCG
CAGGCGGCGGGCAGGCCCGCGCAGGCGAACGGCGCCGCGGCGAGCGCCGCTCAGCCGGCGGCCGCGCCCGTTGCCGCCGC
GCCTGCGCCTGTGGCGGCGGGCGGCGGCACGGTCGACGTGAGGGTGCCGGACATCGGCGACTACAAGGACGTGCCCGTCA
TCGAGATCGCCGTGAAGATCGGCGACACGGTCGAGAAGGAGCAGTCGCTCGTCACGCTCGAGTCCGACAAGGCGACGATG
GACGTGCCGAGCCCGGCCGCGGGCGTCGTCAAGGACATCAAGGTGAAGGTGGGCGACGCGGTGTCGGAAGGCTCGCTGAT
CGTCGTGCTCGAGGCGTCGGGCGCCGCCGCCGCGAGCGCGCCGCAGGCCGCCGCGCCCGCTCAGGCCGCGCCCGCTCCCG
CGGCTGCACCGGCACCCGCGCCTCAGGCTGCACCCGCGCCGCAGGCGGCGCCCGCAGCCGCGCCTGCGCCCGCCGCGAGC
GGCGAGTACCGCCCGAGCCACGCGTCGCCGTCGGTGCGCAAGTTCGCGCGCGAACTCGGTGTCGACGTGTCGCGCGTCGC
GGGCACGGGGCCGAAGAACCGCATCACGAAGGACGACGTCACCGCGTTCGTGAAGGGCGTGATGACGGGGCAGAGGGCGG
CGCCCGCCGCGGCGGCGCCGGCAGGCGGCGGCGAGCTGAATCTGCTGCCGTGGCCGAAGGTCGATTTCTCGAAGTTCGGC
CCGTTCGAGGCGAAGCCGCTGTCGCGGATCAAGAAGATCTCGGGCGCGAACCTGCATCGCAACTGGGTGATGATCCCGCA
CGTCACGAACAACGACGAAGCGGACATCACCGATCTCGAGGCGCTGCGCGTGCAACTGAACAAAGAGCACGAGAAGGCGG
GCGTGAAGTTCACGATGCTCGCGTTCGTGATCAAGGCGGTGGTGGCGGCGCTCAAGAAATTCCCGACCTTCAACGCGAGC
CTCGACGGCGACAACCTCGTCTTCAAGCAGTACTACCACATTGGTTTCGCGGCCGACACGCCGAACGGCCTCGTCGTGCC
GGTGATCCGCGATGCGGACAAGAAGGGGCTCGTCGAGATCGCGAAGGAAATGGCCGAGTTGTCGAAGGCCGCGCGCGAAG
GCAAGCTGAAGCCGGACCAGATGCAGGGCGGCTGCTTCTCGATCTCGTCGCTCGGCGGGATCGGCGGCACGCACTTCACG
CCGATCATCAACGCGCCGGAAGTGGCGATCCTCGGGTTGTCGCGCGGCCAGATGAAGCCGGTGTGGGACGGCAAGCAGTT
CGTGCCGCGCCTCACGCTGCCGCTGTCGCTGTCGTATGACCATCGCGTGATCGACGGCGCGGAAGCCGCGCGGTTCAATG
CGTATCTCGGGGCGTTGCTCGGGGATTTCCGCCGCATCATTCTTTGA

Upstream 100 bases:

>100_bases
TCGGCGAACGAAGGCGGGACGGACGGCGCGGCTTGCGCCTTGCATGGGATCGGAGCGGGCCGCTGCGGCGGCAGCTCCGA
TCGACAGGAGACTGAAACAG

Downstream 100 bases:

>100_bases
TGAGCGTGGGAGTGGCTCGCGGGGGCGTGGGTTGTTTGTCGGTTCGCCATGCCCCGTCGTTGAACCTGTTTTGCCAACGG
TCTATTAGCGTCGCCCCTGC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEVKVKVGDAVSQGSLIVLLDGA
QAAGRPAQANGAAASAAQPAAAPVAAAPAPVAAGGGTVDVRVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATM
DVPSPAAGVVKDIKVKVGDAVSEGSLIVVLEASGAAAASAPQAAAPAQAAPAPAAAPAPAPQAAPAPQAAPAAAPAPAAS
GEYRPSHASPSVRKFARELGVDVSRVAGTGPKNRITKDDVTAFVKGVMTGQRAAPAAAAPAGGGELNLLPWPKVDFSKFG
PFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITDLEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNAS
LDGDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGLVEIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFT
PIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALLGDFRRIIL

Sequences:

>Translated_548_residues
MSQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEVKVKVGDAVSQGSLIVLLDGA
QAAGRPAQANGAAASAAQPAAAPVAAAPAPVAAGGGTVDVRVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATM
DVPSPAAGVVKDIKVKVGDAVSEGSLIVVLEASGAAAASAPQAAAPAQAAPAPAAAPAPAPQAAPAPQAAPAAAPAPAAS
GEYRPSHASPSVRKFARELGVDVSRVAGTGPKNRITKDDVTAFVKGVMTGQRAAPAAAAPAGGGELNLLPWPKVDFSKFG
PFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITDLEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNAS
LDGDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGLVEIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFT
PIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALLGDFRRIIL
>Mature_547_residues
SQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEVKVKVGDAVSQGSLIVLLDGAQ
AAGRPAQANGAAASAAQPAAAPVAAAPAPVAAGGGTVDVRVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATMD
VPSPAAGVVKDIKVKVGDAVSEGSLIVVLEASGAAAASAPQAAAPAQAAPAPAAAPAPAPQAAPAPQAAPAAAPAPAASG
EYRPSHASPSVRKFARELGVDVSRVAGTGPKNRITKDDVTAFVKGVMTGQRAAPAAAAPAGGGELNLLPWPKVDFSKFGP
FEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITDLEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASL
DGDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGLVEIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFTP
IINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALLGDFRRIIL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=448, Percent_Identity=28.5714285714286, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI31711992, Length=418, Percent_Identity=30.8612440191388, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI203098816, Length=483, Percent_Identity=27.7432712215321, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI203098753, Length=427, Percent_Identity=28.3372365339578, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=33.920704845815, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=37.9084967320261, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786305, Length=547, Percent_Identity=53.9305301645338, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1786946, Length=436, Percent_Identity=30.7339449541284, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=427, Percent_Identity=29.2740046838407, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17560088, Length=440, Percent_Identity=32.0454545454545, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=424, Percent_Identity=30.8962264150943, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=306, Percent_Identity=33.6601307189542, Blast_Score=123, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=416, Percent_Identity=29.3269230769231, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6324258, Length=438, Percent_Identity=28.5388127853881, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI18859875, Length=429, Percent_Identity=33.3333333333333, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=31.8965517241379, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=31.8965517241379, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24645909, Length=207, Percent_Identity=34.7826086956522, Blast_Score=118, Evalue=1e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56544; Mature: 56413

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEV
CCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCEEE
KVKVGDAVSQGSLIVLLDGAQAAGRPAQANGAAASAAQPAAAPVAAAPAPVAAGGGTVDV
EEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEE
RVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATMDVPSPAAGVVKDIKVKVGDA
ECCCCCCCCCCCEEEEEEECCCCHHCCCCEEEEECCCCEECCCCCHHHHHHHEEEEECCC
VSEGSLIVVLEASGAAAASAPQAAAPAQAAPAPAAAPAPAPQAAPAPQAAPAAAPAPAAS
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GEYRPSHASPSVRKFARELGVDVSRVAGTGPKNRITKDDVTAFVKGVMTGQRAAPAAAAP
CCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
AGGGELNLLPWPKVDFSKFGPFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITDLE
CCCCEEEECCCCCCCHHHCCCCCCCHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHHH
ALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYYHIGFAADT
HHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECC
PNGLVVPVIRDADKKGLVEIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFT
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCCCCCCCC
PIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALL
EECCCCCEEEEECCCCCCCCCCCCHHHCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHH
GDFRRIIL
HHHHHHCC
>Mature Secondary Structure 
SQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEV
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCEEE
KVKVGDAVSQGSLIVLLDGAQAAGRPAQANGAAASAAQPAAAPVAAAPAPVAAGGGTVDV
EEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEE
RVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATMDVPSPAAGVVKDIKVKVGDA
ECCCCCCCCCCCEEEEEEECCCCHHCCCCEEEEECCCCEECCCCCHHHHHHHEEEEECCC
VSEGSLIVVLEASGAAAASAPQAAAPAQAAPAPAAAPAPAPQAAPAPQAAPAAAPAPAAS
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GEYRPSHASPSVRKFARELGVDVSRVAGTGPKNRITKDDVTAFVKGVMTGQRAAPAAAAP
CCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
AGGGELNLLPWPKVDFSKFGPFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITDLE
CCCCEEEECCCCCCCHHHCCCCCCCHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHHH
ALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYYHIGFAADT
HHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECC
PNGLVVPVIRDADKKGLVEIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFT
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCCCCCCCC
PIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALL
EECCCCCEEEEECCCCCCCCCCCCHHHCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHH
GDFRRIIL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]