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The map label for this gene is miaA [H]

Identifier: 83720662

GI number: 83720662

Start: 1479328

End: 1480302

Strand: Reverse

Name: miaA [H]

Synonym: BTH_I1319

Alternate gene names: 83720662

Gene position: 1480302-1479328 (Counterclockwise)

Preceding gene: 83721397

Following gene: 83718791

Centisome position: 38.86

GC content: 72.51

Gene sequence:

>975_bases
ATGAGCGAATCCAACGCAGCATCCGTGCGCACGGTCGCGTGCCTGCTCGGCCCGACCGCGTCCGGCAAGACGGCCGCCGC
GCTCGCGCTCGCCGCGCGCCGGCCGATCGAGATCGTTAGCGTCGATTCGGCGCTCGTCTACCGCGGCATGGACATCGGCA
CCGCAAAGCCGACGCGCGAAGAGCGCGCGGCCGTGCCGCATCATCTGATCGACATCGTCGACCCGGCCGACGCGTACTCG
GCCGCCGGATTTCGCGCCGATGCGCTGCGCGTCGTCGCCGAGATCGCCGCGCGCGGCCGCACGCCGCTCCTCGCGGGCGG
CACGATGCTCTATTACAAGGCGCTGACGCAAGGGCTGAACGACCTGCCCGCGGCCGATCCGGACGTGCGCGCGACGCTCG
ACGCCGAAGCGGCGCGCGACGGCTGGCCCGCGCTGCACGCGCGCCTCGCGAGCGTCGATCCGGCAACGGCCGCGCGGCTC
GCACCGAACGATGCCCAGCGGATCCAGCGCGCGCTCGAGGTGTATCTGTTGACGGGGCGGCCGATGTCCGCGCTTCTCGC
CGCGCCGCCGCGCAACGACGACGCGGCGGCGGGCCTGCGGTTCGTGCCGGTCGCGCTCGAGCCGTCGGATCGCGCGGTGC
TGCACGCGCGCATCGCCGCGCGCTTCGACGCGATGCTGGAAGCGGGTTTCATCGACGAAGTCGAGCGCCTGCGCCGGCGC
GACGATCTGCATCTCGGGCTGCCGTCGATGCGCTGCGTCGGCTACCGGCAAGCGTGGGAGTATCTCGAAGGCGTGACGGA
CTACCGGATGATGCGCGACAAGGGCATCTTCGCGACCCGTCAGCTCTGCAAGCGGCAGCTCACGTGGCTGCGCGCGATGC
CGGCGCGGATCGTCGTCGATTGCTGTGCGCAAGACGCGACCGCGCGCGCGGTCGACGCGCTCGAACGGGTGCTCGACGGC
CGCACGCCGGCCTGA

Upstream 100 bases:

>100_bases
AGATGGAGGCGACCGAGCGCGCGGATCAATGCAACCACGGCCGGCCGACCTGGTATCAACTGACGCTCGGCGATCTCGAC
AAGCTCTTCATGCGCGGCCA

Downstream 100 bases:

>100_bases
TGCGCGCCCTGCGGCGCGGGCGCTTCAGGCGGCCGACGCGAGCAGGTTCGCGTCGCGCACAAGCCGCCAGCGGCCGTCCG
ACTTGCGCAGGATCGTTAGC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSESNAASVRTVACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRGMDIGTAKPTREERAAVPHHLIDIVDPADAYS
AAGFRADALRVVAEIAARGRTPLLAGGTMLYYKALTQGLNDLPAADPDVRATLDAEAARDGWPALHARLASVDPATAARL
APNDAQRIQRALEVYLLTGRPMSALLAAPPRNDDAAAGLRFVPVALEPSDRAVLHARIAARFDAMLEAGFIDEVERLRRR
DDLHLGLPSMRCVGYRQAWEYLEGVTDYRMMRDKGIFATRQLCKRQLTWLRAMPARIVVDCCAQDATARAVDALERVLDG
RTPA

Sequences:

>Translated_324_residues
MSESNAASVRTVACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRGMDIGTAKPTREERAAVPHHLIDIVDPADAYS
AAGFRADALRVVAEIAARGRTPLLAGGTMLYYKALTQGLNDLPAADPDVRATLDAEAARDGWPALHARLASVDPATAARL
APNDAQRIQRALEVYLLTGRPMSALLAAPPRNDDAAAGLRFVPVALEPSDRAVLHARIAARFDAMLEAGFIDEVERLRRR
DDLHLGLPSMRCVGYRQAWEYLEGVTDYRMMRDKGIFATRQLCKRQLTWLRAMPARIVVDCCAQDATARAVDALERVLDG
RTPA
>Mature_323_residues
SESNAASVRTVACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRGMDIGTAKPTREERAAVPHHLIDIVDPADAYSA
AGFRADALRVVAEIAARGRTPLLAGGTMLYYKALTQGLNDLPAADPDVRATLDAEAARDGWPALHARLASVDPATAARLA
PNDAQRIQRALEVYLLTGRPMSALLAAPPRNDDAAAGLRFVPVALEPSDRAVLHARIAARFDAMLEAGFIDEVERLRRRD
DLHLGLPSMRCVGYRQAWEYLEGVTDYRMMRDKGIFATRQLCKRQLTWLRAMPARIVVDCCAQDATARAVDALERVLDGR
TPA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=309, Percent_Identity=32.3624595469256, Blast_Score=131, Evalue=8e-31,
Organism=Escherichia coli, GI1790613, Length=292, Percent_Identity=53.7671232876712, Blast_Score=308, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI25144712, Length=268, Percent_Identity=26.865671641791, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=302, Percent_Identity=27.4834437086093, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24649830, Length=331, Percent_Identity=30.5135951661631, Blast_Score=133, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 34999; Mature: 34867

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESNAASVRTVACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRGMDIGTAKPTRE
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCCCCHH
ERAAVPHHLIDIVDPADAYSAAGFRADALRVVAEIAARGRTPLLAGGTMLYYKALTQGLN
HHHCCCHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCHH
DLPAADPDVRATLDAEAARDGWPALHARLASVDPATAARLAPNDAQRIQRALEVYLLTGR
CCCCCCCCCEEEECHHHHCCCCHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHEEEECCC
PMSALLAAPPRNDDAAAGLRFVPVALEPSDRAVLHARIAARFDAMLEAGFIDEVERLRRR
CHHHHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
DDLHLGLPSMRCVGYRQAWEYLEGVTDYRMMRDKGIFATRQLCKRQLTWLRAMPARIVVD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
CCAQDATARAVDALERVLDGRTPA
HHCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SESNAASVRTVACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRGMDIGTAKPTRE
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCCCCHH
ERAAVPHHLIDIVDPADAYSAAGFRADALRVVAEIAARGRTPLLAGGTMLYYKALTQGLN
HHHCCCHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCHH
DLPAADPDVRATLDAEAARDGWPALHARLASVDPATAARLAPNDAQRIQRALEVYLLTGR
CCCCCCCCCEEEECHHHHCCCCHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHEEEECCC
PMSALLAAPPRNDDAAAGLRFVPVALEPSDRAVLHARIAARFDAMLEAGFIDEVERLRRR
CHHHHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
DDLHLGLPSMRCVGYRQAWEYLEGVTDYRMMRDKGIFATRQLCKRQLTWLRAMPARIVVD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
CCAQDATARAVDALERVLDGRTPA
HHCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA