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The map label for this gene is rhtB [H]

Identifier: 83720617

GI number: 83720617

Start: 3559890

End: 3560546

Strand: Reverse

Name: rhtB [H]

Synonym: BTH_I3121

Alternate gene names: 83720617

Gene position: 3560546-3559890 (Counterclockwise)

Preceding gene: 83718633

Following gene: 83718748

Centisome position: 93.47

GC content: 69.25

Gene sequence:

>657_bases
ATGAATCTGCATACGTGGTGGCTGTTCGTCGCGACGGTGTTCGTCGTGTCGGCGATTCCCGGTCCGAACATGCTGCTCGT
GATGACGCACGGCGCGCGCCACGGGCTGCGGCGTTCGACGGCGACGATGTCGGGCTGCATGACGGCGCTCGTCGCGATGC
TGTCGGTGTCCGCCGCGGGCCTCGGCGTGTTCCTGGAAGCATGGCCCGCGATGTTCGACGCGCTGCGCTTCGCGGGCGCC
GCGTATCTGATCTACCTCGGCGTGAAGGCGTGGCGCGCGCGCATCGACGGGCAATCCGCGGCGGACGCAGCCGATGCCGC
GCCGCACGCGGGCCGTGCGGGCGCAATCGCCCCGGCGTCGCGCTGGGCGCTCTTTCGCAACGGCTTTCTCGTCGCGGGCA
GCAACCCGAAGGCGATCCTGTTCGCCGCCGCGCTGCTGCCGCAGTTCATCGACGCATCCGCGCCGACGCTGCCGCAGTTC
GGCATACTCGTCGCGACATTCGCGGTGATCGAGGTGAGCTGGTACATCGTCTATGCGTCGTTCGGCACGCGGATCGGCGT
GACGCTGCGCAGCGCGAACGTCGCGAAGGCGTTCAACCGGCTGACCGGCGGCCTGTTCGTCGGTTTCGGCGCGATGATGG
CGCTCGTGCGGCACTGA

Upstream 100 bases:

>100_bases
GTGGCGCGGCGTGCGGCGCGCGGCTGTCCCGCTGCAGACAGCCGCGCGCGTTCCCCGGCATAATCGGCGTTTCCATTTCA
ATTTCGAAAGCGGGGCGGCA

Downstream 100 bases:

>100_bases
GCGGGCGCATCAGCCGACGAGGCGTGGTGCGCCGGCGGCCGCACGCCGCGTGAAGGAACGAAACGCCCCGCGTCGGTGAC
GACGTGGGGCGTCGTCGTTT

Product: amino acid transporter LysE

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTATMSGCMTALVAMLSVSAAGLGVFLEAWPAMFDALRFAGA
AYLIYLGVKAWRARIDGQSAADAADAAPHAGRAGAIAPASRWALFRNGFLVAGSNPKAILFAAALLPQFIDASAPTLPQF
GILVATFAVIEVSWYIVYASFGTRIGVTLRSANVAKAFNRLTGGLFVGFGAMMALVRH

Sequences:

>Translated_218_residues
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTATMSGCMTALVAMLSVSAAGLGVFLEAWPAMFDALRFAGA
AYLIYLGVKAWRARIDGQSAADAADAAPHAGRAGAIAPASRWALFRNGFLVAGSNPKAILFAAALLPQFIDASAPTLPQF
GILVATFAVIEVSWYIVYASFGTRIGVTLRSANVAKAFNRLTGGLFVGFGAMMALVRH
>Mature_218_residues
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTATMSGCMTALVAMLSVSAAGLGVFLEAWPAMFDALRFAGA
AYLIYLGVKAWRARIDGQSAADAADAAPHAGRAGAIAPASRWALFRNGFLVAGSNPKAILFAAALLPQFIDASAPTLPQF
GILVATFAVIEVSWYIVYASFGTRIGVTLRSANVAKAFNRLTGGLFVGFGAMMALVRH

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=218, Percent_Identity=34.8623853211009, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1788099, Length=219, Percent_Identity=31.0502283105023, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23030; Mature: 23030

Theoretical pI: Translated: 11.36; Mature: 11.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTATMSGCMTALVAMLSVSAAG
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVFLEAWPAMFDALRFAGAAYLIYLGVKAWRARIDGQSAADAADAAPHAGRAGAIAPAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCHH
RWALFRNGFLVAGSNPKAILFAAALLPQFIDASAPTLPQFGILVATFAVIEVSWYIVYAS
HHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEEEEE
FGTRIGVTLRSANVAKAFNRLTGGLFVGFGAMMALVRH
CCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTATMSGCMTALVAMLSVSAAG
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVFLEAWPAMFDALRFAGAAYLIYLGVKAWRARIDGQSAADAADAAPHAGRAGAIAPAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCHH
RWALFRNGFLVAGSNPKAILFAAALLPQFIDASAPTLPQFGILVATFAVIEVSWYIVYAS
HHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEEEEE
FGTRIGVTLRSANVAKAFNRLTGGLFVGFGAMMALVRH
CCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]