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The map label for this gene is rhtB [H]

Identifier: 83720421

GI number: 83720421

Start: 3646993

End: 3647616

Strand: Direct

Name: rhtB [H]

Synonym: BTH_I3201

Alternate gene names: 83720421

Gene position: 3646993-3647616 (Clockwise)

Preceding gene: 83718555

Following gene: 83720551

Centisome position: 95.74

GC content: 70.51

Gene sequence:

>624_bases
ATGTCCGTCGAAACCTGGCTCGCCTTCGCCGGCGCGAGCATCGCGCTCCTGATGATTCCCGGCCCCACGATCCTGCTCGT
GATCGGCGATTCGCTCGCGAACCGCAATCGCTCGTCGTGGAGCACCGTCGCCGGCGTCGCGGCGGGCGACACCACCGCGA
TGACGATCTCGCTCGCCGGCGCGGGCGCGCTGCTCGCGATGTCGGCGGCCGCGTTCACCGCGCTCAAGCTCGTCGGCGGC
GGCTATCTCGTCTATCTCGGCATTCGTTCGATTCTGCACGCTCGCCGCGCCGGCACGATGGAAGTGAGCGTCGAGCGCCG
CTCGGCGAAGCGGCGCTTCCTGTCCGCGTGGGCCGTCACCGCGCTGAACCCGAAGAGCATCGTGTTCTTCGTCGCGTTCG
TTCCGCAGTTCATCTCGCCGCACGAGACGTTCGCGCGACAATGCGCGATCCTGCTGCCGACCTTCGTCTGTCTTGCGACG
ATCAACGCGGCCTGCTACGCGCGCGTCGCGCGCTTCGCCGCCGCGCGGCTGACGGGCGCCGCCGCGCAGCGCCGCTTCGG
GTACGGCGGGGGCGCCGCGCTGATCGGCGCGGGCGCGCTGACGCTCGGGATGTCGAGAAGCTGA

Upstream 100 bases:

>100_bases
TATAGAGCATCAATCGTTGAAGAAGAACCGCTTGACAAGCCATCCGCCGCCATGGATCACTAAGGACTTTCGCCCCGCGT
GATCGCGCACGGAGACCATC

Downstream 100 bases:

>100_bases
GGCCGAGGCGCTTCGCGCGGCGGGCCGCTCGAATCCGCGCAGCGAGATGCGCGGCGCGCCGCGTATTCGCGGGGGACGCG
GGCGGGCCGCGTGCCGCCCC

Product: LysE family protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSVETWLAFAGASIALLMIPGPTILLVIGDSLANRNRSSWSTVAGVAAGDTTAMTISLAGAGALLAMSAAAFTALKLVGG
GYLVYLGIRSILHARRAGTMEVSVERRSAKRRFLSAWAVTALNPKSIVFFVAFVPQFISPHETFARQCAILLPTFVCLAT
INAACYARVARFAAARLTGAAAQRRFGYGGGAALIGAGALTLGMSRS

Sequences:

>Translated_207_residues
MSVETWLAFAGASIALLMIPGPTILLVIGDSLANRNRSSWSTVAGVAAGDTTAMTISLAGAGALLAMSAAAFTALKLVGG
GYLVYLGIRSILHARRAGTMEVSVERRSAKRRFLSAWAVTALNPKSIVFFVAFVPQFISPHETFARQCAILLPTFVCLAT
INAACYARVARFAAARLTGAAAQRRFGYGGGAALIGAGALTLGMSRS
>Mature_206_residues
SVETWLAFAGASIALLMIPGPTILLVIGDSLANRNRSSWSTVAGVAAGDTTAMTISLAGAGALLAMSAAAFTALKLVGGG
YLVYLGIRSILHARRAGTMEVSVERRSAKRRFLSAWAVTALNPKSIVFFVAFVPQFISPHETFARQCAILLPTFVCLATI
NAACYARVARFAAARLTGAAAQRRFGYGGGAALIGAGALTLGMSRS

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=202, Percent_Identity=31.1881188118812, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788099, Length=142, Percent_Identity=28.169014084507, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21485; Mature: 21354

Theoretical pI: Translated: 11.74; Mature: 11.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVETWLAFAGASIALLMIPGPTILLVIGDSLANRNRSSWSTVAGVAAGDTTAMTISLAG
CCHHHHHHCCCCEEEEEEECCCEEEEEECCHHCCCCCCCHHHHHEEECCCCEEEEEEECC
AGALLAMSAAAFTALKLVGGGYLVYLGIRSILHARRAGTMEVSVERRSAKRRFLSAWAVT
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHE
ALNPKSIVFFVAFVPQFISPHETFARQCAILLPTFVCLATINAACYARVARFAAARLTGA
ECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAQRRFGYGGGAALIGAGALTLGMSRS
HHHHHCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SVETWLAFAGASIALLMIPGPTILLVIGDSLANRNRSSWSTVAGVAAGDTTAMTISLAG
CHHHHHHCCCCEEEEEEECCCEEEEEECCHHCCCCCCCHHHHHEEECCCCEEEEEEECC
AGALLAMSAAAFTALKLVGGGYLVYLGIRSILHARRAGTMEVSVERRSAKRRFLSAWAVT
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHE
ALNPKSIVFFVAFVPQFISPHETFARQCAILLPTFVCLATINAACYARVARFAAARLTGA
ECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAQRRFGYGGGAALIGAGALTLGMSRS
HHHHHCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]