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The map label for this gene is rlmN [H]

Identifier: 83720408

GI number: 83720408

Start: 2508595

End: 2509731

Strand: Direct

Name: rlmN [H]

Synonym: BTH_I2232

Alternate gene names: 83720408

Gene position: 2508595-2509731 (Clockwise)

Preceding gene: 83719421

Following gene: 83721616

Centisome position: 65.86

GC content: 65.61

Gene sequence:

>1137_bases
ATGGCGAGCGAAACCATCGTCAATCTACTCGACCTCGACGCCGAGGGGCTCGTCGCGTACTGCGGCGGCCTCGGCGAGAA
GGCGTTTCGCGCTAAGCAGCTGCAGCGCTGGATCCATCAATACAATGCAGCCGATTTCGACGGGATGACCGATCTCGCGA
AATCGCTGCGCGAGAAGCTGAAGGGGCGCGCCGTGATCGGCACGCCCGACATCCTGAGCGATCACGTGTCGGCGGACGGC
ACGCGCAAGTGGCTCATCAACGTCGGCAACGGCAATGCGGTCGAGACGGTGTTCATCCCCGAGGAGACGCGCGGCACGCT
GTGCGTGTCGTCGCAGGCGGGGTGTGCGGTCAATTGCCGCTTCTGCTCGACCGGCAAGCAAGGTTTTTCCCGCAATCTGT
CGACGGGCGAGATCGTCGGGCAACTGCGGATGGCGGAATTCGCGCTGCGCGCGTCGCTTGGCCGCGCGCCCGGCCCGAAC
GGCAAGGCCGAGCGGGTGATCACGAATGTCGTGATGATGGGCATGGGCGAGCCGCTTCTCAATTACTCGGCGGTCGTGCC
CGCGATGCGTCTCATGCTCGACGACAACGCGTACGGCCTGTCGCGCCGGCGCGTGACGCTGTCGACGTCGGGCGTCGTGC
CGATGATGGACCGGCTCGGTGCCGAGCTGCCCGTCGCGCTCGCGGTGTCGCTGCACGCGCCGAACGACGCGCTGCGCGAC
GAACTCGTGCCGCTCAACAAGAAGCATCCGCTGCGCGAACTGATGGCCGCGTGCCAGCGCTATCTGAAGGTCGCGCCGCG
TGATTTCATCACGTTCGAATATTGCATGCTCGACGGCGTCAACGACACCGAAGCGCATGCGCGCGAGCTGCTCGCGCTCA
CGCGCGACGTGCCGTGCAAATTCAATCTGATTCCGTTCAATCCGTTCCCGGAATCGGGGCTCCTGCGCTCGAAGACTGAG
CAGATCAAGCGCTTCGCGCAGGTTCTGATCGACGCCGGCGTCGTGACGACGATCCGCAAGACGCGCGGCGACGACATCGA
TGCCGCCTGCGGTCAACTGGCGGGCGCGGTCAAGGACCGCACGCGTCTGGCGGAGCGGACGGGCGCGACGAAGATTATCG
AGGTCCGGGCGGTTTGA

Upstream 100 bases:

>100_bases
AACGGCGCGCGTCGAGATCGCGTTCTTCTTCCCGGAAATGAACGTCTACTCGCGCTAAGCGGGTCGGGGTCCGGCGCGTT
CGGCGGCAAGGCAGGGCATC

Downstream 100 bases:

>100_bases
CGGGGATGCCCGGGCTGGCAGTGAAATACGCGTGAAGCGATCGGGGGCGGCGCACGGCGCCGCGCAGTGTGTGACAACGG
TGGTGTTGCGGGCCCGGCCG

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MASETIVNLLDLDAEGLVAYCGGLGEKAFRAKQLQRWIHQYNAADFDGMTDLAKSLREKLKGRAVIGTPDILSDHVSADG
TRKWLINVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIVGQLRMAEFALRASLGRAPGPN
GKAERVITNVVMMGMGEPLLNYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
ELVPLNKKHPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLALTRDVPCKFNLIPFNPFPESGLLRSKTE
QIKRFAQVLIDAGVVTTIRKTRGDDIDAACGQLAGAVKDRTRLAERTGATKIIEVRAV

Sequences:

>Translated_378_residues
MASETIVNLLDLDAEGLVAYCGGLGEKAFRAKQLQRWIHQYNAADFDGMTDLAKSLREKLKGRAVIGTPDILSDHVSADG
TRKWLINVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIVGQLRMAEFALRASLGRAPGPN
GKAERVITNVVMMGMGEPLLNYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
ELVPLNKKHPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLALTRDVPCKFNLIPFNPFPESGLLRSKTE
QIKRFAQVLIDAGVVTTIRKTRGDDIDAACGQLAGAVKDRTRLAERTGATKIIEVRAV
>Mature_377_residues
ASETIVNLLDLDAEGLVAYCGGLGEKAFRAKQLQRWIHQYNAADFDGMTDLAKSLREKLKGRAVIGTPDILSDHVSADGT
RKWLINVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIVGQLRMAEFALRASLGRAPGPNG
KAERVITNVVMMGMGEPLLNYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRDE
LVPLNKKHPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLALTRDVPCKFNLIPFNPFPESGLLRSKTEQ
IKRFAQVLIDAGVVTTIRKTRGDDIDAACGQLAGAVKDRTRLAERTGATKIIEVRAV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=375, Percent_Identity=53.8666666666667, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004383 [H]

Pfam domain/function: NA

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41174; Mature: 41043

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASETIVNLLDLDAEGLVAYCGGLGEKAFRAKQLQRWIHQYNAADFDGMTDLAKSLREKL
CCCHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRAVIGTPDILSDHVSADGTRKWLINVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCR
CCCEEECCCHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLSTGEIVGQLRMAEFALRASLGRAPGPNGKAERVITNVVMMGMGEPLL
EECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHH
NYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCHHHHH
ELVPLNKKHPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLALTRDVPCK
HCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEHEECCCCCHHHHHHHHHHHHCCCCCE
FNLIPFNPFPESGLLRSKTEQIKRFAQVLIDAGVVTTIRKTRGDDIDAACGQLAGAVKDR
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TRLAERTGATKIIEVRAV
HHHHHHCCCCEEEEEEEC
>Mature Secondary Structure 
ASETIVNLLDLDAEGLVAYCGGLGEKAFRAKQLQRWIHQYNAADFDGMTDLAKSLREKL
CCHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRAVIGTPDILSDHVSADGTRKWLINVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCR
CCCEEECCCHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLSTGEIVGQLRMAEFALRASLGRAPGPNGKAERVITNVVMMGMGEPLL
EECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHH
NYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCHHHHH
ELVPLNKKHPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLALTRDVPCK
HCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEHEECCCCCHHHHHHHHHHHHCCCCCE
FNLIPFNPFPESGLLRSKTEQIKRFAQVLIDAGVVTTIRKTRGDDIDAACGQLAGAVKDR
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TRLAERTGATKIIEVRAV
HHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA