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The map label for this gene is lgrE [H]

Identifier: 83720311

GI number: 83720311

Start: 449772

End: 450551

Strand: Reverse

Name: lgrE [H]

Synonym: BTH_I0404

Alternate gene names: 83720311

Gene position: 450551-449772 (Counterclockwise)

Preceding gene: 83719860

Following gene: 83718532

Centisome position: 11.83

GC content: 68.85

Gene sequence:

>780_bases
ATGGCCTTGCTGCCCGGCACCCTCACCATTCCCGACCGACGCGAACACGCGCGGCACCGGCTGTTGATGTTTCATCACGC
GTGCGGCTCCGCGACGAACTATTTCCGGTGGGCGGCTGCGTTCCCGCCGCATATCGAAGTCTGGCTCGTCGAACTGCCGG
GGCGGGGCCGAAGCCTCAAGCACTCCGCGTTCGACACGCTGCCGCCGCTGCGCGACTACCTGCGCGAGCTCGCCCCCGAG
CTTCCCGCCAACTACGCGATCTTCGGCCACAGCATGGGCGCGCTTGTCGCGTACTGCTTCGCGCACGACATGGCGCAACT
CGGGCGGGCGCCGCGCTGGCTCGGCGTGTCGGGCGCCGACTCGCCGTTCGCCGAATCGCGACGCCGCGCGTTTCCGGTTC
ATCAGCGTCCCGATGCGGCGATCGTCGAGCACCTCGCGACGCTCGGCGGCACGCCGCCCGAAGTCTTCGCGCACGACGAA
CTGCGCACGATGCTGCTGCAACTCGCGAAGGCCGACTTCCGGATCGTCGAGGCATTCTTTCCGCTCGCGTCGGCGACGCC
GTTGCCCGTGCCCGTCACGGTCTTCGCCGGTGCGCAGGACGCCGTGCTGTCCGAGGCCGGACTGCAGGACTGGCAGCGCG
CGTCGACGCTGCCGATCGAGCGCGAAACATTCGACGGCGGACATTTCTATCTGCTCGACACGCCGTCCGCCATCCACCGC
GCGATAGACGCCGCGCTGACGAAGCACGATTTGCCGGCGAGCCTCCCCGTCGTTCTGTAG

Upstream 100 bases:

>100_bases
CCAATCTCCGACCGGGCATCGCCTCGCGTCCCGCGCCCGAGCATTCGCGACGCCGCCGGACCGCCCGAAGATTTGCCTTC
CCTGACTGGAGCGATCGCGC

Downstream 100 bases:

>100_bases
TCGGGAAACGGCCAGATGGCAGCCGGCGCGCGCGGCACGGTGAAACAGGGTGGTGCGCATCGCGCCGGCGCGCCGCGCGA
TTCCGCGCGTGCGTGAATTC

Product: thioesterase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MALLPGTLTIPDRREHARHRLLMFHHACGSATNYFRWAAAFPPHIEVWLVELPGRGRSLKHSAFDTLPPLRDYLRELAPE
LPANYAIFGHSMGALVAYCFAHDMAQLGRAPRWLGVSGADSPFAESRRRAFPVHQRPDAAIVEHLATLGGTPPEVFAHDE
LRTMLLQLAKADFRIVEAFFPLASATPLPVPVTVFAGAQDAVLSEAGLQDWQRASTLPIERETFDGGHFYLLDTPSAIHR
AIDAALTKHDLPASLPVVL

Sequences:

>Translated_259_residues
MALLPGTLTIPDRREHARHRLLMFHHACGSATNYFRWAAAFPPHIEVWLVELPGRGRSLKHSAFDTLPPLRDYLRELAPE
LPANYAIFGHSMGALVAYCFAHDMAQLGRAPRWLGVSGADSPFAESRRRAFPVHQRPDAAIVEHLATLGGTPPEVFAHDE
LRTMLLQLAKADFRIVEAFFPLASATPLPVPVTVFAGAQDAVLSEAGLQDWQRASTLPIERETFDGGHFYLLDTPSAIHR
AIDAALTKHDLPASLPVVL
>Mature_258_residues
ALLPGTLTIPDRREHARHRLLMFHHACGSATNYFRWAAAFPPHIEVWLVELPGRGRSLKHSAFDTLPPLRDYLRELAPEL
PANYAIFGHSMGALVAYCFAHDMAQLGRAPRWLGVSGADSPFAESRRRAFPVHQRPDAAIVEHLATLGGTPPEVFAHDEL
RTMLLQLAKADFRIVEAFFPLASATPLPVPVTVFAGAQDAVLSEAGLQDWQRASTLPIERETFDGGHFYLLDTPSAIHRA
IDAALTKHDLPASLPVVL

Specific function: In the final step of gramicidin biosynthesis, reduces the pentadecapeptide-aldehyde intermediate, that is released from the terminal module of the non-ribosomal peptide synthetase LgrD, to the final product ethanolamine-containing gramicidin [H]

COG id: COG3208

COG function: function code Q; Predicted thioesterase involved in non-ribosomal peptide biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012223
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00975 Thioesterase [H]

EC number: NA

Molecular weight: Translated: 28541; Mature: 28410

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLPGTLTIPDRREHARHRLLMFHHACGSATNYFRWAAAFPPHIEVWLVELPGRGRSLK
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCC
HSAFDTLPPLRDYLRELAPELPANYAIFGHSMGALVAYCFAHDMAQLGRAPRWLGVSGAD
HHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCC
SPFAESRRRAFPVHQRPDAAIVEHLATLGGTPPEVFAHDELRTMLLQLAKADFRIVEAFF
CCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLASATPLPVPVTVFAGAQDAVLSEAGLQDWQRASTLPIERETFDGGHFYLLDTPSAIHR
HHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHH
AIDAALTKHDLPASLPVVL
HHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ALLPGTLTIPDRREHARHRLLMFHHACGSATNYFRWAAAFPPHIEVWLVELPGRGRSLK
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCC
HSAFDTLPPLRDYLRELAPELPANYAIFGHSMGALVAYCFAHDMAQLGRAPRWLGVSGAD
HHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCC
SPFAESRRRAFPVHQRPDAAIVEHLATLGGTPPEVFAHDELRTMLLQLAKADFRIVEAFF
CCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PLASATPLPVPVTVFAGAQDAVLSEAGLQDWQRASTLPIERETFDGGHFYLLDTPSAIHR
HHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHH
AIDAALTKHDLPASLPVVL
HHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA