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The map label for this gene is tig [H]

Identifier: 83720228

GI number: 83720228

Start: 2391295

End: 2392644

Strand: Direct

Name: tig [H]

Synonym: BTH_I2118

Alternate gene names: 83720228

Gene position: 2391295-2392644 (Clockwise)

Preceding gene: 83718638

Following gene: 83719759

Centisome position: 62.78

GC content: 64.59

Gene sequence:

>1350_bases
ATGGCTAACGTTGTTGAAAACCTCGGCAAGCTCGAACGCCGTGTGACGATCTCCCTGCCGAAGGATGTCGTGCAGAAGGA
GATCGACGCCCGTATCCAGAAACTCGCGAAGAACGTGCGCATGCCGGGCTTCCGCCCGGGCAAGGTGCCGCTCAAGATGG
TCGCGCAGCAATACGCGGGCCAGGTCGAGGCGGAGGTGCTGAGCGACAAGATCGGTCAGGAATTCTTCACGATCAGCCGC
TCGGAAAACCTGCGCGTCGCAGGCCAGCCGAGCTTCGCGCCGAAGCAGGATGCGGCGCAGGAGGGCGCGTACGCGTTCGA
CGCGACGTTCGAGGTGTACCCGGAAGTGAAGATCGGCGATCTGGCGACGGCTGAGGTCGAGCGCTCGACGACGACGATCG
GCGACGCCGAGATCGACCGCACGCTCGACATCCTGCGCAAGCAGCGCGTGCACTTCCATGCGCGCGGCGAAGGCGGCGAG
CACGGCGACGGCGGCGCGGACACGTCCGCGCAGAACGGCGACCGCGTGACGGTCGATTTCGTCGGCAAGATCGACGGCGT
CGCGTTCCAGGGCGGCACGGCGGAAGACTTCGTGTTCGTGCTCGGCGAAGGGCGGATGCTGCCCGAATTCGAAACGGCGG
CGCTCGGCCTGAAGACGGGCGAGGCGCGCGAATTCGACCTGAAGTTCCCGGACGACTATCACGGCAAGGACGTGGCGGGC
AAGACCGCGCAATTCACGGTCACGATGAAGAAGGTCGAGTGGCCGCACTTGCCCGCGATCGACGCCGATTTCGCGAAGTC
GCTCGGCATCGAGGACGGCGATCTGACGAAGATGCGCGAGGAGATCAAGGAAAACCTCGGGCGCGAGGCGAAGCGCCGCA
CGCAGTCGATCGTGAAGAACCAGGTGATGGACGCGCTCCTGAAGATCTCCGAGCTCGACGTGCCGAAGGCGCTCATCGAG
CAGGACCAGCAGCGCCTCGTCGAGATGGCCCGTCAGGACCTCGCGCAGCGCGGCGTGCCGAACGCCAAGGACGCGCCGAT
CCCGGTCGAGATGTTCGCCGAGCAGGCCGAGCGCCGCGTGAAGCTCGGTCTCGTGCTCGCCGAGCTGGTGAAGGCGAACG
GCCTCGAGGCGAAGCCCGAGCAGATTCGCGCGGAAGTCGACGAATTCGCGAAGAGCTACGAAGACCCGAAGGAAGTGGTC
CGCTGGTATTATTCGAATCAGCAGCGCCTTGCCGAAATGGAAGCGTTCGTCGTTGAGAGCAACGTCGTCGATTTCGTGCT
GGGCAAGGCGAAGGTGACGGACAAGGAAGTAAGCTTCGAAGCACTGGCGAGCGCAACGGCGCAAGCGTAA

Upstream 100 bases:

>100_bases
CGCATGGGCGAGACGAAGGTGCGCGACGGCGCGTCCGCCGCAAGGCTGGCACGGCAAGGAAAAACCGATTCGCTCGAATA
ATTTTAGGACGATTGAAGCC

Downstream 100 bases:

>100_bases
GTGTCGCTCTAGCGGCGTGCCGGCTGTCGGAGCGACGACGGCCCGCACGCCGTTTTTTCATCGGCGCGGCGCCGCGCGTG
CGGCGTTCTCCGGCTCGTTT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MANVVENLGKLERRVTISLPKDVVQKEIDARIQKLAKNVRMPGFRPGKVPLKMVAQQYAGQVEAEVLSDKIGQEFFTISR
SENLRVAGQPSFAPKQDAAQEGAYAFDATFEVYPEVKIGDLATAEVERSTTTIGDAEIDRTLDILRKQRVHFHARGEGGE
HGDGGADTSAQNGDRVTVDFVGKIDGVAFQGGTAEDFVFVLGEGRMLPEFETAALGLKTGEAREFDLKFPDDYHGKDVAG
KTAQFTVTMKKVEWPHLPAIDADFAKSLGIEDGDLTKMREEIKENLGREAKRRTQSIVKNQVMDALLKISELDVPKALIE
QDQQRLVEMARQDLAQRGVPNAKDAPIPVEMFAEQAERRVKLGLVLAELVKANGLEAKPEQIRAEVDEFAKSYEDPKEVV
RWYYSNQQRLAEMEAFVVESNVVDFVLGKAKVTDKEVSFEALASATAQA

Sequences:

>Translated_449_residues
MANVVENLGKLERRVTISLPKDVVQKEIDARIQKLAKNVRMPGFRPGKVPLKMVAQQYAGQVEAEVLSDKIGQEFFTISR
SENLRVAGQPSFAPKQDAAQEGAYAFDATFEVYPEVKIGDLATAEVERSTTTIGDAEIDRTLDILRKQRVHFHARGEGGE
HGDGGADTSAQNGDRVTVDFVGKIDGVAFQGGTAEDFVFVLGEGRMLPEFETAALGLKTGEAREFDLKFPDDYHGKDVAG
KTAQFTVTMKKVEWPHLPAIDADFAKSLGIEDGDLTKMREEIKENLGREAKRRTQSIVKNQVMDALLKISELDVPKALIE
QDQQRLVEMARQDLAQRGVPNAKDAPIPVEMFAEQAERRVKLGLVLAELVKANGLEAKPEQIRAEVDEFAKSYEDPKEVV
RWYYSNQQRLAEMEAFVVESNVVDFVLGKAKVTDKEVSFEALASATAQA
>Mature_448_residues
ANVVENLGKLERRVTISLPKDVVQKEIDARIQKLAKNVRMPGFRPGKVPLKMVAQQYAGQVEAEVLSDKIGQEFFTISRS
ENLRVAGQPSFAPKQDAAQEGAYAFDATFEVYPEVKIGDLATAEVERSTTTIGDAEIDRTLDILRKQRVHFHARGEGGEH
GDGGADTSAQNGDRVTVDFVGKIDGVAFQGGTAEDFVFVLGEGRMLPEFETAALGLKTGEAREFDLKFPDDYHGKDVAGK
TAQFTVTMKKVEWPHLPAIDADFAKSLGIEDGDLTKMREEIKENLGREAKRRTQSIVKNQVMDALLKISELDVPKALIEQ
DQQRLVEMARQDLAQRGVPNAKDAPIPVEMFAEQAERRVKLGLVLAELVKANGLEAKPEQIRAEVDEFAKSYEDPKEVVR
WYYSNQQRLAEMEAFVVESNVVDFVLGKAKVTDKEVSFEALASATAQA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=444, Percent_Identity=38.7387387387387, Blast_Score=323, Evalue=2e-89,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 49669; Mature: 49538

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVENLGKLERRVTISLPKDVVQKEIDARIQKLAKNVRMPGFRPGKVPLKMVAQQYAG
CCHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
QVEAEVLSDKIGQEFFTISRSENLRVAGQPSFAPKQDAAQEGAYAFDATFEVYPEVKIGD
HHHHHHHHHHHCHHHEEECCCCCEEECCCCCCCCCHHHHHCCCEEEECEEEECCCEEECC
LATAEVERSTTTIGDAEIDRTLDILRKQRVHFHARGEGGEHGDGGADTSAQNGDRVTVDF
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCEEEEEE
VGKIDGVAFQGGTAEDFVFVLGEGRMLPEFETAALGLKTGEAREFDLKFPDDYHGKDVAG
EECCCCEEECCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCEEECCCCCCCCCCCCCC
KTAQFTVTMKKVEWPHLPAIDADFAKSLGIEDGDLTKMREEIKENLGREAKRRTQSIVKN
CCEEEEEEEEECCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVMDALLKISELDVPKALIEQDQQRLVEMARQDLAQRGVPNAKDAPIPVEMFAEQAERRV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
KLGLVLAELVKANGLEAKPEQIRAEVDEFAKSYEDPKEVVRWYYSNQQRLAEMEAFVVES
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHC
NVVDFVLGKAKVTDKEVSFEALASATAQA
CHHHHHHCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANVVENLGKLERRVTISLPKDVVQKEIDARIQKLAKNVRMPGFRPGKVPLKMVAQQYAG
CHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
QVEAEVLSDKIGQEFFTISRSENLRVAGQPSFAPKQDAAQEGAYAFDATFEVYPEVKIGD
HHHHHHHHHHHCHHHEEECCCCCEEECCCCCCCCCHHHHHCCCEEEECEEEECCCEEECC
LATAEVERSTTTIGDAEIDRTLDILRKQRVHFHARGEGGEHGDGGADTSAQNGDRVTVDF
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCEEEEEE
VGKIDGVAFQGGTAEDFVFVLGEGRMLPEFETAALGLKTGEAREFDLKFPDDYHGKDVAG
EECCCCEEECCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCEEECCCCCCCCCCCCCC
KTAQFTVTMKKVEWPHLPAIDADFAKSLGIEDGDLTKMREEIKENLGREAKRRTQSIVKN
CCEEEEEEEEECCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVMDALLKISELDVPKALIEQDQQRLVEMARQDLAQRGVPNAKDAPIPVEMFAEQAERRV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
KLGLVLAELVKANGLEAKPEQIRAEVDEFAKSYEDPKEVVRWYYSNQQRLAEMEAFVVES
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHC
NVVDFVLGKAKVTDKEVSFEALASATAQA
CHHHHHHCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA