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The map label for this gene is gshA-1 [H]

Identifier: 83719941

GI number: 83719941

Start: 91752

End: 93398

Strand: Direct

Name: gshA-1 [H]

Synonym: BTH_I0087

Alternate gene names: 83719941

Gene position: 91752-93398 (Clockwise)

Preceding gene: 83721464

Following gene: 83718701

Centisome position: 2.41

GC content: 67.82

Gene sequence:

>1647_bases
ATGCCGCGGATCGCCGAGCCCGCACTTGCATCAATGCCGAACAACATGACTTCCCGCCAGACAGACTTGTTGCAGCAACG
CCTTGCCGTGTTGAGCACGAGCCCGACGCGCGAGCAGCTGCCTGACGGCCTGCGCGGCATCGAGAAGGAAAGCCTGCGCG
TGACGCGCGACGGGATGATCGCGTTCACGCCGCACCCGCGCGCGCTCGGCTCGGCGCTCACGCATCCGTCGCTCACGACC
GATTATTCGGAGGCGCTCGTCGAGCTGATCACGCCTGCGGAGCGCGACGCCGCGACGACGCTCGAGCGGCTCGACGAACT
GCATCGCTTCGTCTACGCGTCGATCGGCGACGAGATGCTGTGGACGGATTCGATGCCGGGCCTGCTGCCGGCCGACGACG
AAATTCCGATCGCCGACTACGGCACGTCGAACATCGGGCGGCTGAAGACCGTTTACCGGCGCGGCCTCGCGTATCGCTAC
GGGCGCACGATGCAGTGCATCGCGGGGATTCACTACAACTATTCGCTGAGCGAGGAAGTGTGGCGGCGGCTGCACGCGGA
GGAGGGTTCGACCGCGAGCATCGTCGATTACCAGTCGGAGCGCTATCTCGCGCTGATCCGCAATTTCCGTCGCACGAGCT
GGCTGCTGATGTACCTGTTCGGCGCGTCGCCCGCGCTCGATCTGGGTTTCCTGCGCGGCCGGGCGCACAAGCTCGACACG
TTCGACGCCGCGACGCTGTATCGCCCGCACGCGACGAGCCTGCGGATGAGCGACCTCGGCTACTCGAACACGACTGCGCA
GGCCGCGCTGCAGGCCGACTACAACACGCTGTCCGGCTATCTCGACGCGCTGTCGAAGGCGGTCAGCGAGCCGTATCCGC
CGTACGAGGCGATCGGCACGCACCGCGACGGCGAGTGGATTCAGATCAACACGAACGTGCTGCAGATCGAGAACGAGTTC
TACTCGACGATCCGGCCGAAGCGCGTCACGTATTCGGGCGAGCGGCCGCTTCACGCGCTTGCGTCGCGCGGCGTCCAGTA
CATCGAGGTGCGCTGTCTCGACATCGATCCGTTCGAGCCGACGGGCATCGCGCTCGACACCGCGCGCTTTCTCGACGCGT
ACCTGCTCGTCTGCGCGCTCGACGAGAGCGCGCCGCTCACGTGCGACGCGTATCGCGAGTCGAATGCGAACTTCGGCCGC
GTGACGATGGAGGGGCGCAAGCCGGGGCTCGAGCTCGCGCGCAACGGCGCGGCCGTGCCGATGCGCACATGGGCCGACGA
GATCTTCGCGCAGATCGAAGCCGCGGGCCGCGTGCTCGACGACATCCGCGGCGGCGACGCGCATGCGCGCGCGATCGCCG
CGCAGCGCGCGAAGCTCGACGATCCGGAGCTCACGTCTTCCGCGCGCGTGCTGCGCGCGATGCGCGAGAACGGGCAGTCG
TTCCTCGAGTTCGCACGCGCGCAAAGCGCCGCGCATGCGGCGTACTTCCGCGGCCGCCCGCTCGACGAGGCCGCGACGCG
CGATGCGCAGGCGCTCGCCGAGCGTTCGCTCGCCGAGCAGGCCGAGCTCGAAGGCAAGAACGCCGGATCGTTCGACGCGT
TCGTCGCCGCGTATCGCGCATATACGTTGAACCGCTTCAGCGTGTGA

Upstream 100 bases:

>100_bases
CGCGCGAGTCGTCAGGCAGCGCCGAAGGGCAGGTCAAGCGAGCCTTTCGGCGAGATGTTAAAGTGCCCGCTACTGTCAAA
TCCCGCCAGGTGATCCGCGC

Downstream 100 bases:

>100_bases
CGAGCGGCGCGGGCGTTACGGCTGCGCGTCGAGGAAGTCGCGCGCGTTGCATGCGTCGGCGGTGATGCGCAGCGTTTGCA
GCGCGCGTTTCTCGATGCGG

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MPRIAEPALASMPNNMTSRQTDLLQQRLAVLSTSPTREQLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPSLTT
DYSEALVELITPAERDAATTLERLDELHRFVYASIGDEMLWTDSMPGLLPADDEIPIADYGTSNIGRLKTVYRRGLAYRY
GRTMQCIAGIHYNYSLSEEVWRRLHAEEGSTASIVDYQSERYLALIRNFRRTSWLLMYLFGASPALDLGFLRGRAHKLDT
FDAATLYRPHATSLRMSDLGYSNTTAQAALQADYNTLSGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINTNVLQIENEF
YSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIALDTARFLDAYLLVCALDESAPLTCDAYRESNANFGR
VTMEGRKPGLELARNGAAVPMRTWADEIFAQIEAAGRVLDDIRGGDAHARAIAAQRAKLDDPELTSSARVLRAMRENGQS
FLEFARAQSAAHAAYFRGRPLDEAATRDAQALAERSLAEQAELEGKNAGSFDAFVAAYRAYTLNRFSV

Sequences:

>Translated_548_residues
MPRIAEPALASMPNNMTSRQTDLLQQRLAVLSTSPTREQLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPSLTT
DYSEALVELITPAERDAATTLERLDELHRFVYASIGDEMLWTDSMPGLLPADDEIPIADYGTSNIGRLKTVYRRGLAYRY
GRTMQCIAGIHYNYSLSEEVWRRLHAEEGSTASIVDYQSERYLALIRNFRRTSWLLMYLFGASPALDLGFLRGRAHKLDT
FDAATLYRPHATSLRMSDLGYSNTTAQAALQADYNTLSGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINTNVLQIENEF
YSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIALDTARFLDAYLLVCALDESAPLTCDAYRESNANFGR
VTMEGRKPGLELARNGAAVPMRTWADEIFAQIEAAGRVLDDIRGGDAHARAIAAQRAKLDDPELTSSARVLRAMRENGQS
FLEFARAQSAAHAAYFRGRPLDEAATRDAQALAERSLAEQAELEGKNAGSFDAFVAAYRAYTLNRFSV
>Mature_547_residues
PRIAEPALASMPNNMTSRQTDLLQQRLAVLSTSPTREQLPDGLRGIEKESLRVTRDGMIAFTPHPRALGSALTHPSLTTD
YSEALVELITPAERDAATTLERLDELHRFVYASIGDEMLWTDSMPGLLPADDEIPIADYGTSNIGRLKTVYRRGLAYRYG
RTMQCIAGIHYNYSLSEEVWRRLHAEEGSTASIVDYQSERYLALIRNFRRTSWLLMYLFGASPALDLGFLRGRAHKLDTF
DAATLYRPHATSLRMSDLGYSNTTAQAALQADYNTLSGYLDALSKAVSEPYPPYEAIGTHRDGEWIQINTNVLQIENEFY
STIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEPTGIALDTARFLDAYLLVCALDESAPLTCDAYRESNANFGRV
TMEGRKPGLELARNGAAVPMRTWADEIFAQIEAAGRVLDDIRGGDAHARAIAAQRAKLDDPELTSSARVLRAMRENGQSF
LEFARAQSAAHAAYFRGRPLDEAATRDAQALAERSLAEQAELEGKNAGSFDAFVAAYRAYTLNRFSV

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=521, Percent_Identity=41.2667946257198, Blast_Score=381, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 60941; Mature: 60810

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIAEPALASMPNNMTSRQTDLLQQRLAVLSTSPTREQLPDGLRGIEKESLRVTRDGMI
CCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCHHHHCCCHHHEEEEECCEE
AFTPHPRALGSALTHPSLTTDYSEALVELITPAERDAATTLERLDELHRFVYASIGDEML
EECCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
WTDSMPGLLPADDEIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHYNYSLSEEV
ECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHH
WRRLHAEEGSTASIVDYQSERYLALIRNFRRTSWLLMYLFGASPALDLGFLRGRAHKLDT
HHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC
FDAATLYRPHATSLRMSDLGYSNTTAQAALQADYNTLSGYLDALSKAVSEPYPPYEAIGT
CCHHHEECCCCCCEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC
HRDGEWIQINTNVLQIENEFYSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEP
CCCCCEEEEECCEEEECHHHHHHCCCCEEECCCCCCHHHHHHCCCEEEEEEEEECCCCCC
TGIALDTARFLDAYLLVCALDESAPLTCDAYRESNANFGRVTMEGRKPGLELARNGAAVP
CCCCHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCCCCCEEEECCCCCCHHHHCCCCCCC
MRTWADEIFAQIEAAGRVLDDIRGGDAHARAIAAQRAKLDDPELTSSARVLRAMRENGQS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHH
FLEFARAQSAAHAAYFRGRPLDEAATRDAQALAERSLAEQAELEGKNAGSFDAFVAAYRA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
YTLNRFSV
HHHHHCCC
>Mature Secondary Structure 
PRIAEPALASMPNNMTSRQTDLLQQRLAVLSTSPTREQLPDGLRGIEKESLRVTRDGMI
CCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCHHHHCCCHHHEEEEECCEE
AFTPHPRALGSALTHPSLTTDYSEALVELITPAERDAATTLERLDELHRFVYASIGDEML
EECCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
WTDSMPGLLPADDEIPIADYGTSNIGRLKTVYRRGLAYRYGRTMQCIAGIHYNYSLSEEV
ECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHH
WRRLHAEEGSTASIVDYQSERYLALIRNFRRTSWLLMYLFGASPALDLGFLRGRAHKLDT
HHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC
FDAATLYRPHATSLRMSDLGYSNTTAQAALQADYNTLSGYLDALSKAVSEPYPPYEAIGT
CCHHHEECCCCCCEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC
HRDGEWIQINTNVLQIENEFYSTIRPKRVTYSGERPLHALASRGVQYIEVRCLDIDPFEP
CCCCCEEEEECCEEEECHHHHHHCCCCEEECCCCCCHHHHHHCCCEEEEEEEEECCCCCC
TGIALDTARFLDAYLLVCALDESAPLTCDAYRESNANFGRVTMEGRKPGLELARNGAAVP
CCCCHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCCCCCEEEECCCCCCHHHHCCCCCCC
MRTWADEIFAQIEAAGRVLDDIRGGDAHARAIAAQRAKLDDPELTSSARVLRAMRENGQS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHH
FLEFARAQSAAHAAYFRGRPLDEAATRDAQALAERSLAEQAELEGKNAGSFDAFVAAYRA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
YTLNRFSV
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA