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The map label for this gene is pur8 [H]

Identifier: 83719761

GI number: 83719761

Start: 1607095

End: 1608741

Strand: Reverse

Name: pur8 [H]

Synonym: BTH_I1424

Alternate gene names: 83719761

Gene position: 1608741-1607095 (Counterclockwise)

Preceding gene: 83720484

Following gene: 83720254

Centisome position: 42.23

GC content: 71.46

Gene sequence:

>1647_bases
GTGCGGGCATCGACGGCTTCTTCACCGACGATCCCGCGGTCGGCCGCACCGCGGTCGACACGTTCCGCCGGTAAGTTCGT
CTCGCATGCGGGCCGCGCACCGCCGCGGCCCGCGCGGAAACAAGATTCGCCTGTCAGCAACCGGCAGGAAGGCGGCTCAC
AACACGCTTACAATGCAGCCTCCCACCCTCCGGAGTCGTTCTCGATGACACACGGCCTGCACGGACCGAAGCGCTGGTAT
GCGCTCGTCGTTCTCTGTCTGGGCGTGCTGATGATCGTGCTCGACTCGACGATCGTCAACGTCGCGCTGCCGTCGATCGG
CGCGGATCTGCACTTCACCGAAACCGCGCTCGTCTGGATCGTCAACGCCTACATGCTGACGTTCGGCGGCTGCCTGCTGC
TGGGCGGCCGGCTCGGCGACCTGTACGGTCAGCGCCGGATGTTCCTCGCCGGTCTCACGCTCTTCACGCTCGCGTCGCTC
GCCTGCGGGCTCGCGCACACGCAGTTCGTGCTGATCGCCGCGCGCGCGGTGCAGGGGCTCGGCGGCGCGGTCGTATCGGC
CGTCGCGCTGTCGCTCATCATGAATCTCTTCACCGAGCCGGGCGAGCGCGCGAAGGCGATGGGCGTCTACAGCTTCGTCT
GCGCGGGCGGCGGCAGCCTCGGCGTGCTGCTCGGCGGCGTCCTCACGAGCGTGCTCAGCTGGCACTGGATCTTTCTCGTC
AATCTGCCGATCGGCGTTGCCGCCTACGCGCTGTCCGCCGCGCTGCTGCCGAAGGTGCGCGCGCCGGCGGCCGATGCGCG
GCTCGACGTCGCGGGCGCGATCGCCGTGACGGCTTCGCTGATGCTTGCCGTCTACGGCATCGTCAACGGCAATGAAGCGG
GCTGGCTGTCGACGCAAACGATCGCGACGCTCGCGGGCGCGGCCGCGCTGCTCGCGGCGTTCATCGCGATCGAGACGCGC
GTCGCGCATCCGCTGATGCCGCTCGCGCTCGCCGCGCGGCGCAACGTCGCGGTCGCGAATGCGATCGGCGTGCTGTGGGC
GGCCGCGATGTTCGCGTGGTTCTTCCTGTCCGCGCTCTACATGCAGCGCGTGCTCGGCTACGGCCCGCTCCAGGTCGGCC
TGGCGTTTCTGCCCGCGAACCTGATCATGGCCGCGTTCTCGCTCGGCCTGTCCGCGAAGATCGTGATGCGCTTCGGCATC
CGCCGCGCGATCGGCGCCGGGCTCGCCGTCGCGACCGTCGGCCTGGCCCTCTTCGCGCGCGCGCCCGCCGACGGCGGCTT
CGTCGCACACGTGCTGCCGGGCATGATGCTCGTCGGCGTCGGCGCGGGCGTCGCGTTCAACCCGGTGCTGCTCGCCGCGA
TGAGCGACGTCGCGCCGAGCGATTCGGGCCTCGCGTCGGGCATCGTCAACACGTCGTTCATGATGGGCGGCGCGCTCGGC
CTCGCGGTGCTCGCGAGCATCGCGTCCGCGCGCACCGACGCGCTCGCCGCCGCGCACGCGGCGCTGCCCGTCGCGCTCAA
CGGCGGCTATCGCGCCGCGTTCGCCTGCGGCGCGGCGTTCGCCGCGCTGGCCGCCAGTTTCGCGCTCGCGCTGCGCATCC
GCGCACAGTCGGACGCCGCGGCCGAGCTGCACGGCGCCGCGCACTGA

Upstream 100 bases:

>100_bases
CGGCCCGAGAACAACTTCCTGCCGGCGCCGCTGAAGGACGGCGGCGCGGCGAGCGCGCGCAACACGGCGGGATCGGTGCG
CGAGATCCAGGCGTATCTGC

Downstream 100 bases:

>100_bases
GCGGTGCCGTGCAGGAGGCGCCGAAGCACGGCGCCGCTCAGCCATCGCGGGCGGAACGCTTGACCCGACGCTCGCGGCCG
CCCGTCGCCTCGGCCGCGCG

Product: EmrB/QacA family drug resistance transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MRASTASSPTIPRSAAPRSTRSAGKFVSHAGRAPPRPARKQDSPVSNRQEGGSQHAYNAASHPPESFSMTHGLHGPKRWY
ALVVLCLGVLMIVLDSTIVNVALPSIGADLHFTETALVWIVNAYMLTFGGCLLLGGRLGDLYGQRRMFLAGLTLFTLASL
ACGLAHTQFVLIAARAVQGLGGAVVSAVALSLIMNLFTEPGERAKAMGVYSFVCAGGGSLGVLLGGVLTSVLSWHWIFLV
NLPIGVAAYALSAALLPKVRAPAADARLDVAGAIAVTASLMLAVYGIVNGNEAGWLSTQTIATLAGAAALLAAFIAIETR
VAHPLMPLALAARRNVAVANAIGVLWAAAMFAWFFLSALYMQRVLGYGPLQVGLAFLPANLIMAAFSLGLSAKIVMRFGI
RRAIGAGLAVATVGLALFARAPADGGFVAHVLPGMMLVGVGAGVAFNPVLLAAMSDVAPSDSGLASGIVNTSFMMGGALG
LAVLASIASARTDALAAAHAALPVALNGGYRAAFACGAAFAALAASFALALRIRAQSDAAAELHGAAH

Sequences:

>Translated_548_residues
MRASTASSPTIPRSAAPRSTRSAGKFVSHAGRAPPRPARKQDSPVSNRQEGGSQHAYNAASHPPESFSMTHGLHGPKRWY
ALVVLCLGVLMIVLDSTIVNVALPSIGADLHFTETALVWIVNAYMLTFGGCLLLGGRLGDLYGQRRMFLAGLTLFTLASL
ACGLAHTQFVLIAARAVQGLGGAVVSAVALSLIMNLFTEPGERAKAMGVYSFVCAGGGSLGVLLGGVLTSVLSWHWIFLV
NLPIGVAAYALSAALLPKVRAPAADARLDVAGAIAVTASLMLAVYGIVNGNEAGWLSTQTIATLAGAAALLAAFIAIETR
VAHPLMPLALAARRNVAVANAIGVLWAAAMFAWFFLSALYMQRVLGYGPLQVGLAFLPANLIMAAFSLGLSAKIVMRFGI
RRAIGAGLAVATVGLALFARAPADGGFVAHVLPGMMLVGVGAGVAFNPVLLAAMSDVAPSDSGLASGIVNTSFMMGGALG
LAVLASIASARTDALAAAHAALPVALNGGYRAAFACGAAFAALAASFALALRIRAQSDAAAELHGAAH
>Mature_548_residues
MRASTASSPTIPRSAAPRSTRSAGKFVSHAGRAPPRPARKQDSPVSNRQEGGSQHAYNAASHPPESFSMTHGLHGPKRWY
ALVVLCLGVLMIVLDSTIVNVALPSIGADLHFTETALVWIVNAYMLTFGGCLLLGGRLGDLYGQRRMFLAGLTLFTLASL
ACGLAHTQFVLIAARAVQGLGGAVVSAVALSLIMNLFTEPGERAKAMGVYSFVCAGGGSLGVLLGGVLTSVLSWHWIFLV
NLPIGVAAYALSAALLPKVRAPAADARLDVAGAIAVTASLMLAVYGIVNGNEAGWLSTQTIATLAGAAALLAAFIAIETR
VAHPLMPLALAARRNVAVANAIGVLWAAAMFAWFFLSALYMQRVLGYGPLQVGLAFLPANLIMAAFSLGLSAKIVMRFGI
RRAIGAGLAVATVGLALFARAPADGGFVAHVLPGMMLVGVGAGVAFNPVLLAAMSDVAPSDSGLASGIVNTSFMMGGALG
LAVLASIASARTDALAAAHAALPVALNGGYRAAFACGAAFAALAASFALALRIRAQSDAAAELHGAAH

Specific function: May be involved in active puromycin efflux energized by a proton-dependent electrochemical gradient. In addition, it could be implicated in secreting N-acetylpuromycin, the last intermediate of the puromycin biosynthesis pathway, to the environment [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=432, Percent_Identity=29.8611111111111, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI1790195, Length=407, Percent_Identity=28.5012285012285, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1789042, Length=438, Percent_Identity=26.4840182648402, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788710, Length=315, Percent_Identity=28.2539682539683, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788392, Length=421, Percent_Identity=29.2161520190024, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790146, Length=169, Percent_Identity=29.585798816568, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6324954, Length=455, Percent_Identity=27.9120879120879, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6323520, Length=533, Percent_Identity=23.4521575984991, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319770, Length=399, Percent_Identity=24.3107769423559, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=161, Percent_Identity=31.055900621118, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=154, Percent_Identity=30.5194805194805, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=429, Percent_Identity=23.7762237762238, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55849; Mature: 55849

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASTASSPTIPRSAAPRSTRSAGKFVSHAGRAPPRPARKQDSPVSNRQEGGSQHAYNAA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCC
SHPPESFSMTHGLHGPKRWYALVVLCLGVLMIVLDSTIVNVALPSIGADLHFTETALVWI
CCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VNAYMLTFGGCLLLGGRLGDLYGQRRMFLAGLTLFTLASLACGLAHTQFVLIAARAVQGL
HHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GGAVVSAVALSLIMNLFTEPGERAKAMGVYSFVCAGGGSLGVLLGGVLTSVLSWHWIFLV
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEE
NLPIGVAAYALSAALLPKVRAPAADARLDVAGAIAVTASLMLAVYGIVNGNEAGWLSTQT
ECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IATLAGAAALLAAFIAIETRVAHPLMPLALAARRNVAVANAIGVLWAAAMFAWFFLSALY
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
MQRVLGYGPLQVGLAFLPANLIMAAFSLGLSAKIVMRFGIRRAIGAGLAVATVGLALFAR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHC
APADGGFVAHVLPGMMLVGVGAGVAFNPVLLAAMSDVAPSDSGLASGIVNTSFMMGGALG
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LAVLASIASARTDALAAAHAALPVALNGGYRAAFACGAAFAALAASFALALRIRAQSDAA
HHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHH
AELHGAAH
HHHCCCCC
>Mature Secondary Structure
MRASTASSPTIPRSAAPRSTRSAGKFVSHAGRAPPRPARKQDSPVSNRQEGGSQHAYNAA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCC
SHPPESFSMTHGLHGPKRWYALVVLCLGVLMIVLDSTIVNVALPSIGADLHFTETALVWI
CCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VNAYMLTFGGCLLLGGRLGDLYGQRRMFLAGLTLFTLASLACGLAHTQFVLIAARAVQGL
HHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GGAVVSAVALSLIMNLFTEPGERAKAMGVYSFVCAGGGSLGVLLGGVLTSVLSWHWIFLV
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEE
NLPIGVAAYALSAALLPKVRAPAADARLDVAGAIAVTASLMLAVYGIVNGNEAGWLSTQT
ECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
IATLAGAAALLAAFIAIETRVAHPLMPLALAARRNVAVANAIGVLWAAAMFAWFFLSALY
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
MQRVLGYGPLQVGLAFLPANLIMAAFSLGLSAKIVMRFGIRRAIGAGLAVATVGLALFAR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHC
APADGGFVAHVLPGMMLVGVGAGVAFNPVLLAAMSDVAPSDSGLASGIVNTSFMMGGALG
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LAVLASIASARTDALAAAHAALPVALNGGYRAAFACGAAFAALAASFALALRIRAQSDAA
HHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHH
AELHGAAH
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7916693 [H]