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The map label for this gene is braD [H]

Identifier: 83719683

GI number: 83719683

Start: 3790582

End: 3791466

Strand: Reverse

Name: braD [H]

Synonym: BTH_I3328

Alternate gene names: 83719683

Gene position: 3791466-3790582 (Counterclockwise)

Preceding gene: 83718951

Following gene: 83719557

Centisome position: 99.53

GC content: 64.63

Gene sequence:

>885_bases
ATGGACATCTTCGGCATTCCGATGTCGGCGATGCTGAGCCAGTTGCTGCTCGGACTCGTCAACGGCTCGTTCTACGCGAT
CCTGAGCCTCGGGCTCGCGGTGATATTCGGGCTGCTCAACGTGATCAACTTCGCGCACGGCGCGCTCTTCATGCTGGGCG
CGATGCTCGCGTGGATGGGGCTCGACTATCTCGGCTTGCCGTACTGGGCGATGCTCGTGCTCGCGCCCGTCGTCGTCGGT
CTGTTCGGGATACTGATCGAGCGCTCGATGCTGCGCTGGCTCTACAAGCTCGATCACCTGTACGGCTTGCTGCTGACGTT
CGGGCTCACGCTGGTCGTCGAAGGCGTGTTCCGCGCGATTTACGGCTCGTCCGGCCAGCCGTACGACGTGCCCGAGCTGC
TCGCAGGCGCGACCAACGTCGGCTTCATGTTCCTGCCGAACTATCGCGCGTGGGTCGTCGTCGCATCGCTCGCGGTGTGC
TTCGCGACGTGGTTCGTGATCGAGAAGACGCGGCTCGGCGCCTACCTGCGCGCGGGCACCGAAAATCCGAAGCTCGTCGA
GGCGTTCGGCGTGAACGTGCCGATGATGGTCACGCTGACCTACGGCTTCGGCGTCGCGCTGGCCGCGTTCGCCGGCGTGC
TCGCGGCGCCCGTGATCCAGGTGTCGCCGCTGATGGGGCAGCCGATGATCATCACCGTGTTCGCGGTCGTCGTGATCGGC
GGCATGGGCTCGATTCTCGGCTCGATCTTGACGGGCCTGATGCTCGGCGTGGTCGAGGGCTTCACGCGCGTGTTCTATCC
GGAGGCGTCGGCGACGGTCGTGTTCGTCATCATGGCGATCGTGCTGCTGATTCGCCCGGCAGGTTTGTTCGGCAAGGAAA
GATGA

Upstream 100 bases:

>100_bases
GAATGCACGCGCGACGGCGGCCCGAATGCCGCTGTTGTGACCATTTGGTTGCATGCGCAACGTTTTTTGACCTGACACTG
ACTGATTGACGGCAGATTCG

Downstream 100 bases:

>100_bases
TGCAGAGAAAAGCGCTCTACGGGCTGTTGCTGGCCGGCCTGCTCGTCGCGCCGTTCGTCGGCGCGTATCCGGTGTTCATG
ATGAAGGTGCTGTGCTTCGC

Product: branched-chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MDIFGIPMSAMLSQLLLGLVNGSFYAILSLGLAVIFGLLNVINFAHGALFMLGAMLAWMGLDYLGLPYWAMLVLAPVVVG
LFGILIERSMLRWLYKLDHLYGLLLTFGLTLVVEGVFRAIYGSSGQPYDVPELLAGATNVGFMFLPNYRAWVVVASLAVC
FATWFVIEKTRLGAYLRAGTENPKLVEAFGVNVPMMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGQPMIITVFAVVVIG
GMGSILGSILTGLMLGVVEGFTRVFYPEASATVVFVIMAIVLLIRPAGLFGKER

Sequences:

>Translated_294_residues
MDIFGIPMSAMLSQLLLGLVNGSFYAILSLGLAVIFGLLNVINFAHGALFMLGAMLAWMGLDYLGLPYWAMLVLAPVVVG
LFGILIERSMLRWLYKLDHLYGLLLTFGLTLVVEGVFRAIYGSSGQPYDVPELLAGATNVGFMFLPNYRAWVVVASLAVC
FATWFVIEKTRLGAYLRAGTENPKLVEAFGVNVPMMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGQPMIITVFAVVVIG
GMGSILGSILTGLMLGVVEGFTRVFYPEASATVVFVIMAIVLLIRPAGLFGKER
>Mature_294_residues
MDIFGIPMSAMLSQLLLGLVNGSFYAILSLGLAVIFGLLNVINFAHGALFMLGAMLAWMGLDYLGLPYWAMLVLAPVVVG
LFGILIERSMLRWLYKLDHLYGLLLTFGLTLVVEGVFRAIYGSSGQPYDVPELLAGATNVGFMFLPNYRAWVVVASLAVC
FATWFVIEKTRLGAYLRAGTENPKLVEAFGVNVPMMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGQPMIITVFAVVVIG
GMGSILGSILTGLMLGVVEGFTRVFYPEASATVVFVIMAIVLLIRPAGLFGKER

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=305, Percent_Identity=28.1967213114754, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 31534; Mature: 31534

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIFGIPMSAMLSQLLLGLVNGSFYAILSLGLAVIFGLLNVINFAHGALFMLGAMLAWMG
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDYLGLPYWAMLVLAPVVVGLFGILIERSMLRWLYKLDHLYGLLLTFGLTLVVEGVFRAI
CHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGSSGQPYDVPELLAGATNVGFMFLPNYRAWVVVASLAVCFATWFVIEKTRLGAYLRAGT
HCCCCCCCCHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENPKLVEAFGVNVPMMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGQPMIITVFAVVVIG
CCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GMGSILGSILTGLMLGVVEGFTRVFYPEASATVVFVIMAIVLLIRPAGLFGKER
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDIFGIPMSAMLSQLLLGLVNGSFYAILSLGLAVIFGLLNVINFAHGALFMLGAMLAWMG
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDYLGLPYWAMLVLAPVVVGLFGILIERSMLRWLYKLDHLYGLLLTFGLTLVVEGVFRAI
CHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGSSGQPYDVPELLAGATNVGFMFLPNYRAWVVVASLAVCFATWFVIEKTRLGAYLRAGT
HCCCCCCCCHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENPKLVEAFGVNVPMMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGQPMIITVFAVVVIG
CCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GMGSILGSILTGLMLGVVEGFTRVFYPEASATVVFVIMAIVLLIRPAGLFGKER
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]