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The map label for this gene is rph [H]

Identifier: 83719437

GI number: 83719437

Start: 1783943

End: 1784674

Strand: Reverse

Name: rph [H]

Synonym: BTH_I1584

Alternate gene names: 83719437

Gene position: 1784674-1783943 (Counterclockwise)

Preceding gene: 83719322

Following gene: 83720085

Centisome position: 46.85

GC content: 70.9

Gene sequence:

>732_bases
ATGACGAATTCCTCCCCACGCCCGAGCGGCCGCCGCGCCGATCAACTGCGCGACGTGCGCATCACGCGCCACTACACGAA
GCATGCGGAAGGCTCGGTGCTCGTCGAGTTCGGCGAGACGAAAGTGATCTGCACGGCGAGCGTCGTCGAGCGCGTGCCCG
AGTTCCTGCGCGAGCGCGGCCAGGGCTGGCTCACCGCCGAATACGGGATGCTGCCGCGCGCGACCCACACGCGCAGCGAC
CGGGAAGCGGCGCGCGGCAAGCAGACGGGCCGCACGCAGGAAATTCAGCGCCTGATCGGCCGCGCGCTGCGCGCGGTATT
CGATCTGAACGCGCTCGGCCCGCGCACGCTGCACCTCGATTGCGACGTGATCCAGGCCGACGGCGGCACGCGCACGGCGA
GCATCACGGGCGCGTTCGTCGCCGCGCACGACGCGGTGACGAAGCTCGTCGCGGCAGGCAAGATCGCGCGCTCCCCGATC
ACCGATTACGTCGCGGCGATCTCGGTCGGCGTGTTCGGCGGCGCGCCCGTGCTCGATCTCGACTACGACGAAGACTCCGC
GTGCGACACCGACATGAACGTCGTGATGACGGGCGCGGGCGGTTTCGTCGAGGTGCAGGGCACCGCGGAAGGCGTGCCGT
TCTCGCGCGCCGAGATGAACGCGCTCCTCGACCTCGCGCAGGCCGGCATCGGCGAGCTCGTGCGGCTGCAGCGCGCCGCG
CTGGAGGCCTGA

Upstream 100 bases:

>100_bases
GGCGCGAAACATGCATTATCCCATTTTTGCGGGCCGGACCCGCCGGGCCGATGCGCCGCGGGCGCGGTAAAATCCGGGTT
TCTCCCAGCACATTGCCCCG

Downstream 100 bases:

>100_bases
CGATGTCGCACGCATCGACCGAGGCCGTGGCCTCGCGCATCGTCCTCGCATCGAACAACGCCGGCAAGCTGCGCGAGTTC
GCCGCCCTCTTCTCGACGGC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTNSSPRPSGRRADQLRDVRITRHYTKHAEGSVLVEFGETKVICTASVVERVPEFLRERGQGWLTAEYGMLPRATHTRSD
REAARGKQTGRTQEIQRLIGRALRAVFDLNALGPRTLHLDCDVIQADGGTRTASITGAFVAAHDAVTKLVAAGKIARSPI
TDYVAAISVGVFGGAPVLDLDYDEDSACDTDMNVVMTGAGGFVEVQGTAEGVPFSRAEMNALLDLAQAGIGELVRLQRAA
LEA

Sequences:

>Translated_243_residues
MTNSSPRPSGRRADQLRDVRITRHYTKHAEGSVLVEFGETKVICTASVVERVPEFLRERGQGWLTAEYGMLPRATHTRSD
REAARGKQTGRTQEIQRLIGRALRAVFDLNALGPRTLHLDCDVIQADGGTRTASITGAFVAAHDAVTKLVAAGKIARSPI
TDYVAAISVGVFGGAPVLDLDYDEDSACDTDMNVVMTGAGGFVEVQGTAEGVPFSRAEMNALLDLAQAGIGELVRLQRAA
LEA
>Mature_242_residues
TNSSPRPSGRRADQLRDVRITRHYTKHAEGSVLVEFGETKVICTASVVERVPEFLRERGQGWLTAEYGMLPRATHTRSDR
EAARGKQTGRTQEIQRLIGRALRAVFDLNALGPRTLHLDCDVIQADGGTRTASITGAFVAAHDAVTKLVAAGKIARSPIT
DYVAAISVGVFGGAPVLDLDYDEDSACDTDMNVVMTGAGGFVEVQGTAEGVPFSRAEMNALLDLAQAGIGELVRLQRAAL
EA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=67.9425837320574, Blast_Score=288, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI71981632, Length=185, Percent_Identity=27.5675675675676, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 26035; Mature: 25904

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSSPRPSGRRADQLRDVRITRHYTKHAEGSVLVEFGETKVICTASVVERVPEFLRERG
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHC
QGWLTAEYGMLPRATHTRSDREAARGKQTGRTQEIQRLIGRALRAVFDLNALGPRTLHLD
CCEEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
CDVIQADGGTRTASITGAFVAAHDAVTKLVAAGKIARSPITDYVAAISVGVFGGAPVLDL
EEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEE
DYDEDSACDTDMNVVMTGAGGFVEVQGTAEGVPFSRAEMNALLDLAQAGIGELVRLQRAA
CCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEA
HCC
>Mature Secondary Structure 
TNSSPRPSGRRADQLRDVRITRHYTKHAEGSVLVEFGETKVICTASVVERVPEFLRERG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHC
QGWLTAEYGMLPRATHTRSDREAARGKQTGRTQEIQRLIGRALRAVFDLNALGPRTLHLD
CCEEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
CDVIQADGGTRTASITGAFVAAHDAVTKLVAAGKIARSPITDYVAAISVGVFGGAPVLDL
EEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEE
DYDEDSACDTDMNVVMTGAGGFVEVQGTAEGVPFSRAEMNALLDLAQAGIGELVRLQRAA
CCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA