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The map label for this gene is phoB [H]

Identifier: 83719375

GI number: 83719375

Start: 3180411

End: 3181112

Strand: Reverse

Name: phoB [H]

Synonym: BTH_I2768

Alternate gene names: 83719375

Gene position: 3181112-3180411 (Counterclockwise)

Preceding gene: 83720340

Following gene: 83720326

Centisome position: 83.51

GC content: 64.81

Gene sequence:

>702_bases
ATGCCCAGCAACATTCTCGTCATCGAAGATGAACCCGCGATTTCCGAATTGATTTCGGTGAACCTTCAGCATGCGGGGCA
TTGTCCGATTCGCGCATACAACGCGGAGCAGGCGCAGAGCCTGATCAGCGACGTGCTGCCCGATCTGGTGCTGCTCGACT
GGATGTTGCCGGGCAAGTCGGGCATCGCGTTCGCGCGCGACCTGCGCAACAACGAGCGCACGAAGCACATCCCGATCATC
ATGCTGACCGCGCGCGGCGACGAGCAGGACAAGGTGCTCGGCCTCGAGATCGGCGCGGACGACTACGTGACGAAGCCGTT
CTCGCCGAAGGAGCTGATGGCGCGGATCAAGGCGGTGTTGCGCCGGCGCGCGCCGCAGCTGACGGAGGACGTCGTGTCGA
TCAACGGGCTGCGGCTCGACCCGGCGACGCATCGCGTCGCGGCGCAATCCGACGGCAGCGAGATCAAGCTCGATCTCGGT
CCGACCGAGTTTCGCCTGCTGCATTTCTTCATGACCCATCCGGAGCGCGTCCACAGCCGCACGCAACTGCTCGACCAGGT
CTGGGGCGATCACGTGTTCGTCGAGGAGCGCACGGTCGACGTGCACATCAAGCGGCTGCGCGCCGCGCTCAAGCCCGCCG
GCTGCGATGCTATGATCGAAACCGTGCGCGGCAGCGGCTACCGGCTCGCGAAACACGCGTAA

Upstream 100 bases:

>100_bases
ATTTACATCGTGAAGGGCACCGACGTGCGGCACCAGCCGCGCGACATGCTCGATCGCGAAGCCAACAGCTAACTGCCGAC
AAGGACGGAACGAGGTGCCG

Downstream 100 bases:

>100_bases
CGGTTGCGCGGCGCGAAGGGTCAGCGCCGCCGCGTGACATCGTTTTCCTCTTTCGTCGGGCGCCGAATCATGAACATCAT
CTGGGCGCGCTTTCTGGTGT

Product: phosphate regulon transcriptional regulatory protein PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MPSNILVIEDEPAISELISVNLQHAGHCPIRAYNAEQAQSLISDVLPDLVLLDWMLPGKSGIAFARDLRNNERTKHIPII
MLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVLRRRAPQLTEDVVSINGLRLDPATHRVAAQSDGSEIKLDLG
PTEFRLLHFFMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKHA

Sequences:

>Translated_233_residues
MPSNILVIEDEPAISELISVNLQHAGHCPIRAYNAEQAQSLISDVLPDLVLLDWMLPGKSGIAFARDLRNNERTKHIPII
MLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVLRRRAPQLTEDVVSINGLRLDPATHRVAAQSDGSEIKLDLG
PTEFRLLHFFMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKHA
>Mature_232_residues
PSNILVIEDEPAISELISVNLQHAGHCPIRAYNAEQAQSLISDVLPDLVLLDWMLPGKSGIAFARDLRNNERTKHIPIIM
LTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVLRRRAPQLTEDVVSINGLRLDPATHRVAAQSDGSEIKLDLGP
TEFRLLHFFMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKHA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=230, Percent_Identity=57.3913043478261, Blast_Score=280, Evalue=4e-77,
Organism=Escherichia coli, GI1788394, Length=231, Percent_Identity=41.1255411255411, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1789809, Length=232, Percent_Identity=37.5, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1790860, Length=228, Percent_Identity=38.5964912280702, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI1786784, Length=225, Percent_Identity=36, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=34.6153846153846, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI87082012, Length=224, Percent_Identity=34.8214285714286, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786911, Length=236, Percent_Identity=35.5932203389831, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=39.3162393162393, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=33.1838565022422, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787229, Length=237, Percent_Identity=33.7552742616034, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1789402, Length=227, Percent_Identity=34.3612334801762, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1790552, Length=228, Percent_Identity=32.4561403508772, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI145693140, Length=244, Percent_Identity=30.7377049180328, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1788550, Length=113, Percent_Identity=34.5132743362832, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26084; Mature: 25953

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSNILVIEDEPAISELISVNLQHAGHCPIRAYNAEQAQSLISDVLPDLVLLDWMLPGKS
CCCCEEEECCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC
GIAFARDLRNNERTKHIPIIMLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVL
CHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTEDVVSINGLRLDPATHRVAAQSDGSEIKLDLGPTEFRLLHFFMTHPERVHSR
HHCCCHHHHHHHHCCCEEECCHHHHEEECCCCCEEEEECCCHHHHHHHHHHCCHHHHHHH
TQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKHA
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCC
>Mature Secondary Structure 
PSNILVIEDEPAISELISVNLQHAGHCPIRAYNAEQAQSLISDVLPDLVLLDWMLPGKS
CCCEEEECCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC
GIAFARDLRNNERTKHIPIIMLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVL
CHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTEDVVSINGLRLDPATHRVAAQSDGSEIKLDLGPTEFRLLHFFMTHPERVHSR
HHCCCHHHHHHHHCCCEEECCHHHHEEECCCCCEEEEECCCHHHHHHHHHHCCHHHHHHH
TQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKHA
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]