Click here to switch to the map view.

The map label for this gene is priA [H]

Identifier: 83719363

GI number: 83719363

Start: 3761420

End: 3763714

Strand: Reverse

Name: priA [H]

Synonym: BTH_I3303

Alternate gene names: 83719363

Gene position: 3763714-3761420 (Counterclockwise)

Preceding gene: 83719923

Following gene: 83721387

Centisome position: 98.81

GC content: 72.72

Gene sequence:

>2295_bases
ATGCCGGCATTCGGCGGTGCCGCACCGATGGCTGACGCGTTCGTGCGCGTCGCGCTCGATCATCCGCTGCCGACGCTGTT
CGATTACCGCTATCGCGGCGACGCCCCGGCCGCGCCGGGAATGCTCGTGCAGGTGCCGTTCGGCCGGCGCAGCGCGGTCG
GGCTCGTGTGCGAAGTGACCGCTCACACGGACGTTCCCGCGAACAAGCTGAAGGCGGTCGACGCAGTCTGCGCGGAGCTG
CCGCCGCTGTCGCGGCGGTGGCTCGACCTCGTCGCGTTCGCGGCCGACTACTATCAGCGCGGACTCGGCGAAGTCGCGCT
GCCCGCGCTGCCTCAGGCGCTGCGCGACGTGACGCGCTGGGGACGGCTGCTCGCGCCCGAGTTGCGCTACCGGCTGCTCG
ATGCCGGCCGCGCCGCGCTGCCGGCCGCGCTGCCCGCGCGCGGCGCGGCGCTGCGCCGGCTCGCGCAGGCGCTCGCGGAC
GCGCCCGCGCTGTCGCTCGCCGAGGTGCGCGCGCTGCACCCGAAGGCCGTCGCGGTGCTCGACGAGTGGCAGGCGCAAGG
CTGGGCCGCATGCGACGAGATCGCGATCACGCATGCGCACGCGCAGTTCGTCGGCAACCCGGCGCCGATCGCCGGATGGC
GCGCGGTTGCGTCGCGGCCGCAACTGACCGATCAGCAGGCCGACGCGCTCGACGCGATCGCGTCGGCGGACGGCTTCGTG
CCGTTCCTGCTGCACGGCGTGACGGGCAGCGGCAAGACCGAGGTGTATCTGCGCGCGCTTGCCGCGCTGCTCGAGCGGCG
GTCCGACGCGCAGGCGCTCGTCCTCGTGCCCGAGATCAACCTGACGCCGCAGTTCGAGGCGGCATTCCGAGCGCGCTTCG
CGGCGACGCTGCCCGCCGATGCGATCGTCACGCTGCATAGCGGCCTTGCCGAAGGCGAGCGCGCGCGCCACTGGCTCGCC
GCGCATACCGGCCGTGCGCGGATCGTGCTCGGCACGCGGCTCGCGGTGCTCGCGTCGCTGCCGGCGCTCGCGATGATCGT
CGTCGACGAGGAGCACGAGCCGGCCTACAAGCAACAGGAAGGGTTGCGCTATTCGGCGCGCGATCTCGCCGTGTGGCGGG
CGAAGCAGTGTGCGATTCCGGTCGTGCTCGGCTCGGCGACGCCGTCGCTCGAAACCTGGTGGCAGGCCGAGCAGGGCCGC
TACCGGCGCCTCACGCTGACGAAGCGCGCGGTCGCCGATGCGGCGCTGCCGACGGTGCGGCTCGTCGATCTCGAGGAGGA
GCGGCGGCGCGGGCGCGGGTCGATCGGCGGGCTGTCGGGGCCGCTCGTCGCCGCGTTGAAGGCGCGGCTCGAGCGCGGCG
AGCAGAGCCTCGTGTTCCTGAACCGGCGCGGCTATGCGCCCGCGCTCGCATGCGACGCGTGCGGCTGGGTCGCAGGCTGC
CCGCGATGCAGCGCGTACGTCGTGATGCACAAGCCCGAGCACGCGGTGCGCTGCCACCACTGCGGCTGGGAGGCGCGGAT
TCCGCGGTCGTGCCCGGAATGCGGAAACGTCGACATCGCGCCGCTCGGCCGCGGCACGCAACGCGTCGAGGAGACGCTCG
CGCTCGCCGTGCCGGGCGCGCGGGTCCTGCGTATCGATGCGGACAGCACGCGCCGCAAGGGCAGCGCGCAGGCGCTTTTC
TCGGACGTGCACGCGGGCGAGGTCGACATACTCGTCGGCACGCAGATGATCGCGAAGGGGCACGATTTCCAGCGGGTGTC
GCTTGTCGGCGTGCTGAACGCGGATACCGCGCTCTTCTCGCACGATTTCCGCGCGAGCGAGCGGCTATTCGCGCAACTGA
TGCAGGTGAGCGGCCGCGCGGGGCGCGCGGGGCTGCCGGGCGAGGTGCTGATTCAGACGCGCTACCCGCGCCACGCGCTC
TATCACGCGCTCGCGCGTCAGGATTACGTGGGGTTCGCCGGCGCGACGCTCGGCGAGCGGCGCGACGCGCATCTGCCGCC
GTTCGTCTATCAGGCGCTGTTACGCGCCGAGGGGCGCACGCTCGAGGCGGCGCTCGTGTTCCTGCAGCAGGCGGCCGACG
CTTTTTCGGCGCTGCCGGGCGCGGAGCGCGTGACTGTCTATGACGCCGTGCCGATGACGATCGTCAAGGTGGCGAACGTC
CACCGGGCGCAACTGTTGATCGAGAGCGCGTCGCGCGCGGCGCTTCAGCACACGCTGCGCGCGTGGCAGCCGCTGTTGCG
CGAGCTGAAGGGCGTGCTGCGCTGGAGCGTCGAGGTCGATCCGCTCGACATCTGA

Upstream 100 bases:

>100_bases
GCGGACGCGCCGCGATTCTTAATGAGAATCCAAAACTTAGCGCCGAAAGTGATGTTTCACTTTAACTTCGCGAGCGTCAT
GGGTGTCTGGCCTGCCGGGC

Downstream 100 bases:

>100_bases
CGCCGGCGCGTCACTCGTGCGCTTCGCGCAGGCCGGGAATCCGCGCGCGGCGCTTGCCTTTCTTCGCGCCGTGCGCGTAC
CCGAGCGCCTGGAAGAACAC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 764; Mature: 763

Protein sequence:

>764_residues
MPAFGGAAPMADAFVRVALDHPLPTLFDYRYRGDAPAAPGMLVQVPFGRRSAVGLVCEVTAHTDVPANKLKAVDAVCAEL
PPLSRRWLDLVAFAADYYQRGLGEVALPALPQALRDVTRWGRLLAPELRYRLLDAGRAALPAALPARGAALRRLAQALAD
APALSLAEVRALHPKAVAVLDEWQAQGWAACDEIAITHAHAQFVGNPAPIAGWRAVASRPQLTDQQADALDAIASADGFV
PFLLHGVTGSGKTEVYLRALAALLERRSDAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGLAEGERARHWLA
AHTGRARIVLGTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQCAIPVVLGSATPSLETWWQAEQGR
YRRLTLTKRAVADAALPTVRLVDLEEERRRGRGSIGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGC
PRCSAYVVMHKPEHAVRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRVEETLALAVPGARVLRIDADSTRRKGSAQALF
SDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLIQTRYPRHAL
YHALARQDYVGFAGATLGERRDAHLPPFVYQALLRAEGRTLEAALVFLQQAADAFSALPGAERVTVYDAVPMTIVKVANV
HRAQLLIESASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI

Sequences:

>Translated_764_residues
MPAFGGAAPMADAFVRVALDHPLPTLFDYRYRGDAPAAPGMLVQVPFGRRSAVGLVCEVTAHTDVPANKLKAVDAVCAEL
PPLSRRWLDLVAFAADYYQRGLGEVALPALPQALRDVTRWGRLLAPELRYRLLDAGRAALPAALPARGAALRRLAQALAD
APALSLAEVRALHPKAVAVLDEWQAQGWAACDEIAITHAHAQFVGNPAPIAGWRAVASRPQLTDQQADALDAIASADGFV
PFLLHGVTGSGKTEVYLRALAALLERRSDAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGLAEGERARHWLA
AHTGRARIVLGTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQCAIPVVLGSATPSLETWWQAEQGR
YRRLTLTKRAVADAALPTVRLVDLEEERRRGRGSIGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGC
PRCSAYVVMHKPEHAVRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRVEETLALAVPGARVLRIDADSTRRKGSAQALF
SDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLIQTRYPRHAL
YHALARQDYVGFAGATLGERRDAHLPPFVYQALLRAEGRTLEAALVFLQQAADAFSALPGAERVTVYDAVPMTIVKVANV
HRAQLLIESASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI
>Mature_763_residues
PAFGGAAPMADAFVRVALDHPLPTLFDYRYRGDAPAAPGMLVQVPFGRRSAVGLVCEVTAHTDVPANKLKAVDAVCAELP
PLSRRWLDLVAFAADYYQRGLGEVALPALPQALRDVTRWGRLLAPELRYRLLDAGRAALPAALPARGAALRRLAQALADA
PALSLAEVRALHPKAVAVLDEWQAQGWAACDEIAITHAHAQFVGNPAPIAGWRAVASRPQLTDQQADALDAIASADGFVP
FLLHGVTGSGKTEVYLRALAALLERRSDAQALVLVPEINLTPQFEAAFRARFAATLPADAIVTLHSGLAEGERARHWLAA
HTGRARIVLGTRLAVLASLPALAMIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQCAIPVVLGSATPSLETWWQAEQGRY
RRLTLTKRAVADAALPTVRLVDLEEERRRGRGSIGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGCP
RCSAYVVMHKPEHAVRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRVEETLALAVPGARVLRIDADSTRRKGSAQALFS
DVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLIQTRYPRHALY
HALARQDYVGFAGATLGERRDAHLPPFVYQALLRAEGRTLEAALVFLQQAADAFSALPGAERVTVYDAVPMTIVKVANVH
RAQLLIESASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=759, Percent_Identity=41.501976284585, Blast_Score=492, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82681; Mature: 82550

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAFGGAAPMADAFVRVALDHPLPTLFDYRYRGDAPAAPGMLVQVPFGRRSAVGLVCEVT
CCCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEE
AHTDVPANKLKAVDAVCAELPPLSRRWLDLVAFAADYYQRGLGEVALPALPQALRDVTRW
ECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GRLLAPELRYRLLDAGRAALPAALPARGAALRRLAQALADAPALSLAEVRALHPKAVAVL
HHHHCHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHH
DEWQAQGWAACDEIAITHAHAQFVGNPAPIAGWRAVASRPQLTDQQADALDAIASADGFV
HHHHCCCCCCHHHHHEEEHHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCH
PFLLHGVTGSGKTEVYLRALAALLERRSDAQALVLVPEINLTPQFEAAFRARFAATLPAD
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCH
AIVTLHSGLAEGERARHWLAAHTGRARIVLGTRLAVLASLPALAMIVVDEEHEPAYKQQE
HHHHHHHCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHCCCEEEEEEECCCCCCHHHHH
GLRYSARDLAVWRAKQCAIPVVLGSATPSLETWWQAEQGRYRRLTLTKRAVADAALPTVR
CCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCEEE
LVDLEEERRRGRGSIGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGC
EECCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCHHCCC
PRCSAYVVMHKPEHAVRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRVEETLALAVPGA
CCCCEEEEEECCCHHHEEECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCC
RVLRIDADSTRRKGSAQALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFS
EEEEECCCCCCCCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHH
HDFRASERLFAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDYVGFAGATLGER
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCC
RDAHLPPFVYQALLRAEGRTLEAALVFLQQAADAFSALPGAERVTVYDAVPMTIVKVANV
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
HRAQLLIESASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC
>Mature Secondary Structure 
PAFGGAAPMADAFVRVALDHPLPTLFDYRYRGDAPAAPGMLVQVPFGRRSAVGLVCEVT
CCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEE
AHTDVPANKLKAVDAVCAELPPLSRRWLDLVAFAADYYQRGLGEVALPALPQALRDVTRW
ECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GRLLAPELRYRLLDAGRAALPAALPARGAALRRLAQALADAPALSLAEVRALHPKAVAVL
HHHHCHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHH
DEWQAQGWAACDEIAITHAHAQFVGNPAPIAGWRAVASRPQLTDQQADALDAIASADGFV
HHHHCCCCCCHHHHHEEEHHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCH
PFLLHGVTGSGKTEVYLRALAALLERRSDAQALVLVPEINLTPQFEAAFRARFAATLPAD
HHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCH
AIVTLHSGLAEGERARHWLAAHTGRARIVLGTRLAVLASLPALAMIVVDEEHEPAYKQQE
HHHHHHHCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHCCCEEEEEEECCCCCCHHHHH
GLRYSARDLAVWRAKQCAIPVVLGSATPSLETWWQAEQGRYRRLTLTKRAVADAALPTVR
CCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHCCCEEE
LVDLEEERRRGRGSIGGLSGPLVAALKARLERGEQSLVFLNRRGYAPALACDACGWVAGC
EECCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCHHCCC
PRCSAYVVMHKPEHAVRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRVEETLALAVPGA
CCCCEEEEEECCCHHHEEECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCC
RVLRIDADSTRRKGSAQALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFS
EEEEECCCCCCCCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHH
HDFRASERLFAQLMQVSGRAGRAGLPGEVLIQTRYPRHALYHALARQDYVGFAGATLGER
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCC
RDAHLPPFVYQALLRAEGRTLEAALVFLQQAADAFSALPGAERVTVYDAVPMTIVKVANV
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
HRAQLLIESASRAALQHTLRAWQPLLRELKGVLRWSVEVDPLDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]