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The map label for this gene is shiA [H]

Identifier: 83719345

GI number: 83719345

Start: 2062272

End: 2063603

Strand: Direct

Name: shiA [H]

Synonym: BTH_I1835

Alternate gene names: 83719345

Gene position: 2062272-2063603 (Clockwise)

Preceding gene: 83721611

Following gene: 83720275

Centisome position: 54.14

GC content: 70.8

Gene sequence:

>1332_bases
ATGACATCCCAGCACGATGACCGCGCCGTTCGCGCGCCGGTCGGCCAGCCCGCGCGCGCCGCGCTCGCGGCGTTCGTCGG
CACGACGATCGAGTGGTACGACTTCTACCTTTACGCAACCGCGTCCGCGCTCATCTTCGGCAAGCTGTTCTTTCCGTCGG
GCGAGCCGTTCTTCAGCACGCTCGCGTCGTTCGGCACGTTCGCGGTCGGCTTTTTCGCGCGGCCGTTCGGCGGCCTCGTG
TTCGGCCATCTCGGCGACCGGATCGGCCGCAAGAAGGCGCTCGTCGCGACGCTCGTCATCATGGCGGTCGGCACGGTCGG
CATCGGCCTGTTGCCTTCATACGCGCAGGCGGGCGTCGTCGCGCCCGTCGTGCTCGTCGTGCTGCGCATCGCGCAGGGCA
TCGCGATCGGCGGCGAGTGGGGCGGCGCGGTGCTGATGGCGAGCGAGCACGCGAGCGCGGACAAGCGCACGTTCGTCGCG
TCGTTCGCGCAGCTCGGCAGCCCGGCCGGGCTGATTCTCGCGCTGCTCGCGTTTCGTCTCGTCGCGGGCATCGAGCACGG
CGCGTTCCTCGCATGGGGCTGGCGGCTGCCGTTTCTCGCGAGCGCGGCGCTGCTCGCGGTCGGGCTCGCGATTCGCGCGG
GCGTCGCCGAATCGCCGGAATTCGCGCGCGTGAAGGCCGAGCGGCGCACGGCCGCGCTGCCCGTGGCCGACGTGGTGCGC
GACGCATGGCGCACGCTGCTGCTCTGTCTCGGCGCGAACGTGATCGGCGTCGCGGGCGCGTGGTTCGTCAACACGTTCAT
GCTGAACTACACGACGCAGACGCTCGGGCTCGATCGCGCGCTGATCCTCGATTGCCTGTTCGTCGTCGCGTTCATCCAGC
TCGGCACGCAGTTCGCGTCAGGCTGGTTCGCGCAGCGCATCGGCATCGGGCGCTTCCTGAAGACGGCCGCCGCGCTCGCG
ATCGCGTCGCCGTATCCGATGTTCGCGCTCGTGTCGACGGGGCGGCCCGTCGCGATCGTCGTGGGCATCGCGATCGCGGT
GATGTGCATGTCGAGCTCGTACGCGGTGATGGCGGGCTTCATGTCGAGCGCGTTCGACGTGCGCGTCCGCTATTCGGCGA
TCTCGCTGTCGTATCAGCTTTGCGCGGCGCTCGCGGGCGGGCTCACGCCGCTCGCGGGCACGCTGCTCGCGCACCGTTTC
GCCGGCGAGTGGTGGCCGCTCGCCGCGTTCTACAGCGCGCTCGCGGCGGTGTCGCTCGGGTGCATCGCGACGCTCGAGCG
CGACAAGCGAAGCGACGCCGGCGCGCCCGGCGGTGCGCTCGCGAAGCCTTGA

Upstream 100 bases:

>100_bases
GCGCGCCGCCTGTCCGGCGGCGACCGAACCCGGAGCGATCCGGCCCGGCCGGTGAAGGCGCGCGCGAGTCGCGCCACCGG
CAAGAGACATGGAGACAACG

Downstream 100 bases:

>100_bases
CGCGCCGCGCGCGAAGCGCGGCCGCGGCGCCCGCAAAAAAGCCTCGGGCAGCAATCGCTGCCCGAGGCTTTTTGTTCGAG
TGCGCAACGCGCCAGTGCGC

Product: major facilitator family transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MTSQHDDRAVRAPVGQPARAALAAFVGTTIEWYDFYLYATASALIFGKLFFPSGEPFFSTLASFGTFAVGFFARPFGGLV
FGHLGDRIGRKKALVATLVIMAVGTVGIGLLPSYAQAGVVAPVVLVVLRIAQGIAIGGEWGGAVLMASEHASADKRTFVA
SFAQLGSPAGLILALLAFRLVAGIEHGAFLAWGWRLPFLASAALLAVGLAIRAGVAESPEFARVKAERRTAALPVADVVR
DAWRTLLLCLGANVIGVAGAWFVNTFMLNYTTQTLGLDRALILDCLFVVAFIQLGTQFASGWFAQRIGIGRFLKTAAALA
IASPYPMFALVSTGRPVAIVVGIAIAVMCMSSSYAVMAGFMSSAFDVRVRYSAISLSYQLCAALAGGLTPLAGTLLAHRF
AGEWWPLAAFYSALAAVSLGCIATLERDKRSDAGAPGGALAKP

Sequences:

>Translated_443_residues
MTSQHDDRAVRAPVGQPARAALAAFVGTTIEWYDFYLYATASALIFGKLFFPSGEPFFSTLASFGTFAVGFFARPFGGLV
FGHLGDRIGRKKALVATLVIMAVGTVGIGLLPSYAQAGVVAPVVLVVLRIAQGIAIGGEWGGAVLMASEHASADKRTFVA
SFAQLGSPAGLILALLAFRLVAGIEHGAFLAWGWRLPFLASAALLAVGLAIRAGVAESPEFARVKAERRTAALPVADVVR
DAWRTLLLCLGANVIGVAGAWFVNTFMLNYTTQTLGLDRALILDCLFVVAFIQLGTQFASGWFAQRIGIGRFLKTAAALA
IASPYPMFALVSTGRPVAIVVGIAIAVMCMSSSYAVMAGFMSSAFDVRVRYSAISLSYQLCAALAGGLTPLAGTLLAHRF
AGEWWPLAAFYSALAAVSLGCIATLERDKRSDAGAPGGALAKP
>Mature_442_residues
TSQHDDRAVRAPVGQPARAALAAFVGTTIEWYDFYLYATASALIFGKLFFPSGEPFFSTLASFGTFAVGFFARPFGGLVF
GHLGDRIGRKKALVATLVIMAVGTVGIGLLPSYAQAGVVAPVVLVVLRIAQGIAIGGEWGGAVLMASEHASADKRTFVAS
FAQLGSPAGLILALLAFRLVAGIEHGAFLAWGWRLPFLASAALLAVGLAIRAGVAESPEFARVKAERRTAALPVADVVRD
AWRTLLLCLGANVIGVAGAWFVNTFMLNYTTQTLGLDRALILDCLFVVAFIQLGTQFASGWFAQRIGIGRFLKTAAALAI
ASPYPMFALVSTGRPVAIVVGIAIAVMCMSSSYAVMAGFMSSAFDVRVRYSAISLSYQLCAALAGGLTPLAGTLLAHRFA
GEWWPLAAFYSALAAVSLGCIATLERDKRSDAGAPGGALAKP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=416, Percent_Identity=36.0576923076923, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI1789941, Length=394, Percent_Identity=34.5177664974619, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1790550, Length=413, Percent_Identity=30.7506053268765, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788942, Length=393, Percent_Identity=30.5343511450382, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI87082231, Length=226, Percent_Identity=26.1061946902655, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46339; Mature: 46208

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQHDDRAVRAPVGQPARAALAAFVGTTIEWYDFYLYATASALIFGKLFFPSGEPFFST
CCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LASFGTFAVGFFARPFGGLVFGHLGDRIGRKKALVATLVIMAVGTVGIGLLPSYAQAGVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHH
APVVLVVLRIAQGIAIGGEWGGAVLMASEHASADKRTFVASFAQLGSPAGLILALLAFRL
HHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
VAGIEHGAFLAWGWRLPFLASAALLAVGLAIRAGVAESPEFARVKAERRTAALPVADVVR
HHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHH
DAWRTLLLCLGANVIGVAGAWFVNTFMLNYTTQTLGLDRALILDCLFVVAFIQLGTQFAS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GWFAQRIGIGRFLKTAAALAIASPYPMFALVSTGRPVAIVVGIAIAVMCMSSSYAVMAGF
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHH
MSSAFDVRVRYSAISLSYQLCAALAGGLTPLAGTLLAHRFAGEWWPLAAFYSALAAVSLG
HHHHHHEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
CIATLERDKRSDAGAPGGALAKP
HHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSQHDDRAVRAPVGQPARAALAAFVGTTIEWYDFYLYATASALIFGKLFFPSGEPFFST
CCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
LASFGTFAVGFFARPFGGLVFGHLGDRIGRKKALVATLVIMAVGTVGIGLLPSYAQAGVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHH
APVVLVVLRIAQGIAIGGEWGGAVLMASEHASADKRTFVASFAQLGSPAGLILALLAFRL
HHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
VAGIEHGAFLAWGWRLPFLASAALLAVGLAIRAGVAESPEFARVKAERRTAALPVADVVR
HHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHH
DAWRTLLLCLGANVIGVAGAWFVNTFMLNYTTQTLGLDRALILDCLFVVAFIQLGTQFAS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GWFAQRIGIGRFLKTAAALAIASPYPMFALVSTGRPVAIVVGIAIAVMCMSSSYAVMAGF
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHH
MSSAFDVRVRYSAISLSYQLCAALAGGLTPLAGTLLAHRFAGEWWPLAAFYSALAAVSLG
HHHHHHEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
CIATLERDKRSDAGAPGGALAKP
HHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]