BacMap

Definition	Burkholderia thailandensis E264 chromosome chromosome I, complete sequence.
Accession	NC_007651
Length	3,809,201

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The map label for this gene is paaF [C]

Identifier: 83719344

GI number: 83719344

Start: 647610

End: 648389

Strand: Direct

Name: paaF [C]

Synonym: BTH_I0567

Alternate gene names: 83719344

Gene position: 647610-648389 (Clockwise)

Preceding gene: 83721513

Following gene: 83720731

Centisome position: 17.0

GC content: 67.69

Gene sequence:

>780_bases
ATGGAGACGGCTCAAATGGATATCCAGGTCGATAACGCGGGCGGCGTGCTGACGATCACGCTTGCGCGTCCGTCGAAGAA
GAACGCGATCACCGCGGCGATGTACCAGACGATGGCCGACGCGCTCGCGGCCGCTCAGGACGACAAATCGGTGCGCGCGA
TCCTGATTCGCGGCAGCGACGGCAATTTCAGCGCGGGCAACGATCTCGACGATTTCATGAAGGCGCCGCCGAAGGACGAG
AACGCGCCCGTGTTCCAGTTCCTGCGCCAGATCGCGAGCGCGCAGAAGCCGATCGTCGCGGCGGTCGCGGGCGTCGCGGT
CGGCATCGGCGTGACGATGTTGCTGCACTGCGATCTCGTCTACGCGGCCGACACCGCGCAGTTGTCGCTGCCGTTCGCGC
AGCTCGGCCTGTGCCCGGAGGCGGCGTCGAGCCTGCTGCTGCCGCGCGTTGCCGGCCATCAGGTCGCTGCGGAAAAGCTG
CTGCTCGGCGAACCGTTCGATGCGCTCGAGGCGCACCGGATCGGCCTCGTGAACCGCGTGCTGCCCGCCGCGGATCTCGA
CGCGTTCGCGGCCGGGCAAGCGGCGAAGCTCGTCGCGCTGCCGGCGTCGTCGCTGCGCGCGACGAAGGCGTTCCTCAAGA
AGGGGGCCGGCACGGAAATCGCCGCGCGGATGTCGGAGGAAGGCGATCAGTTCGGCAGAATGCTGCGCTCGCCCGAAGTG
CGCGAGGCGATCACCGCGTTCTTCGAGAAGCGCAAGCCGGATTTCCGGCAGTTCGACTGA

Upstream 100 bases:

>100_bases
GGGCATCATCGAGCGGCTCTGATCGGAGCGCGCGACGCATGACGACGGTGCGCGGGCGGCGAGCTCGCGCACCGTTTTTT
TTGATCGAGTGAAAACAGCC

Downstream 100 bases:

>100_bases
GCGCCATTGAGGCGGTGCGATGCGGCGCGGCGGCTCGTGCCGGCCGCATCGCGCGCGCGGATGCCGGCGCGGCGGCATGC
CGCACGTCGCCTTCGCGCGT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
METAQMDIQVDNAGGVLTITLARPSKKNAITAAMYQTMADALAAAQDDKSVRAILIRGSDGNFSAGNDLDDFMKAPPKDE
NAPVFQFLRQIASAQKPIVAAVAGVAVGIGVTMLLHCDLVYAADTAQLSLPFAQLGLCPEAASSLLLPRVAGHQVAAEKL
LLGEPFDALEAHRIGLVNRVLPAADLDAFAAGQAAKLVALPASSLRATKAFLKKGAGTEIAARMSEEGDQFGRMLRSPEV
REAITAFFEKRKPDFRQFD

Sequences:

>Translated_259_residues
METAQMDIQVDNAGGVLTITLARPSKKNAITAAMYQTMADALAAAQDDKSVRAILIRGSDGNFSAGNDLDDFMKAPPKDE
NAPVFQFLRQIASAQKPIVAAVAGVAVGIGVTMLLHCDLVYAADTAQLSLPFAQLGLCPEAASSLLLPRVAGHQVAAEKL
LLGEPFDALEAHRIGLVNRVLPAADLDAFAAGQAAKLVALPASSLRATKAFLKKGAGTEIAARMSEEGDQFGRMLRSPEV
REAITAFFEKRKPDFRQFD
>Mature_259_residues
METAQMDIQVDNAGGVLTITLARPSKKNAITAAMYQTMADALAAAQDDKSVRAILIRGSDGNFSAGNDLDDFMKAPPKDE
NAPVFQFLRQIASAQKPIVAAVAGVAVGIGVTMLLHCDLVYAADTAQLSLPFAQLGLCPEAASSLLLPRVAGHQVAAEKL
LLGEPFDALEAHRIGLVNRVLPAADLDAFAAGQAAKLVALPASSLRATKAFLKKGAGTEIAARMSEEGDQFGRMLRSPEV
REAITAFFEKRKPDFRQFD

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI45643119, Length=250, Percent_Identity=34.8, Blast_Score=130, Evalue=9e-31,
Organism=Homo sapiens, GI260275230, Length=250, Percent_Identity=34.8, Blast_Score=130, Evalue=9e-31,
Organism=Homo sapiens, GI260274832, Length=250, Percent_Identity=34.8, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI194097323, Length=243, Percent_Identity=29.6296296296296, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=28.4552845528455, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4757968, Length=257, Percent_Identity=27.2373540856031, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI49355787, Length=257, Percent_Identity=27.2373540856031, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI25453481, Length=257, Percent_Identity=26.4591439688716, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI51538573, Length=257, Percent_Identity=26.4591439688716, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI4757966, Length=246, Percent_Identity=26.8292682926829, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI51538581, Length=246, Percent_Identity=26.8292682926829, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI68989263, Length=154, Percent_Identity=33.7662337662338, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI70995211, Length=182, Percent_Identity=31.3186813186813, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI221136756, Length=257, Percent_Identity=23.7354085603113, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI221136753, Length=257, Percent_Identity=23.7354085603113, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI221307494, Length=257, Percent_Identity=23.7354085603113, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI116235487, Length=215, Percent_Identity=27.906976744186, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI150378541, Length=247, Percent_Identity=28.7449392712551, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4502327, Length=251, Percent_Identity=27.8884462151394, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI37594469, Length=153, Percent_Identity=30.0653594771242, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI37594471, Length=153, Percent_Identity=30.0653594771242, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI62530384, Length=254, Percent_Identity=23.2283464566929, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI157694516, Length=247, Percent_Identity=23.8866396761134, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI213417737, Length=247, Percent_Identity=23.8866396761134, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=244, Percent_Identity=31.9672131147541, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1787660, Length=248, Percent_Identity=33.4677419354839, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1788597, Length=251, Percent_Identity=31.4741035856574, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI221142681, Length=256, Percent_Identity=33.203125, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=26.2357414448669, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1790281, Length=232, Percent_Identity=25, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1788682, Length=189, Percent_Identity=26.4550264550265, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=33.9920948616601, Blast_Score=130, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17549921, Length=182, Percent_Identity=40.1098901098901, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17554946, Length=256, Percent_Identity=30.859375, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=30.6122448979592, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=207, Percent_Identity=29.951690821256, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=238, Percent_Identity=27.7310924369748, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=210, Percent_Identity=25.7142857142857, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=189, Percent_Identity=30.6878306878307, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=24.4444444444444, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=192, Percent_Identity=31.25, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=192, Percent_Identity=31.25, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=192, Percent_Identity=31.25, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=185, Percent_Identity=27.5675675675676, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323314, Length=195, Percent_Identity=25.6410256410256, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24654903, Length=246, Percent_Identity=31.7073170731707, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=31.7073170731707, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=31.7073170731707, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=31.2, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=25.0980392156863, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19920382, Length=200, Percent_Identity=28, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21357171, Length=206, Percent_Identity=27.1844660194175, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24650670, Length=205, Percent_Identity=26.8292682926829, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27449; Mature: 27449

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METAQMDIQVDNAGGVLTITLARPSKKNAITAAMYQTMADALAAAQDDKSVRAILIRGSD
CCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
GNFSAGNDLDDFMKAPPKDENAPVFQFLRQIASAQKPIVAAVAGVAVGIGVTMLLHCDLV
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
YAADTAQLSLPFAQLGLCPEAASSLLLPRVAGHQVAAEKLLLGEPFDALEAHRIGLVNRV
EECCCCCEECCHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LPAADLDAFAAGQAAKLVALPASSLRATKAFLKKGAGTEIAARMSEEGDQFGRMLRSPEV
CCCCCCHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHH
REAITAFFEKRKPDFRQFD
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
METAQMDIQVDNAGGVLTITLARPSKKNAITAAMYQTMADALAAAQDDKSVRAILIRGSD
CCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
GNFSAGNDLDDFMKAPPKDENAPVFQFLRQIASAQKPIVAAVAGVAVGIGVTMLLHCDLV
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
YAADTAQLSLPFAQLGLCPEAASSLLLPRVAGHQVAAEKLLLGEPFDALEAHRIGLVNRV
EECCCCCEECCHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LPAADLDAFAAGQAAKLVALPASSLRATKAFLKKGAGTEIAARMSEEGDQFGRMLRSPEV
CCCCCCHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHH
REAITAFFEKRKPDFRQFD
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA