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The map label for this gene is citA [H]

Identifier: 83719245

GI number: 83719245

Start: 250931

End: 252223

Strand: Direct

Name: citA [H]

Synonym: BTH_I0219

Alternate gene names: 83719245

Gene position: 250931-252223 (Clockwise)

Preceding gene: 83720124

Following gene: 83721240

Centisome position: 6.59

GC content: 67.52

Gene sequence:

>1293_bases
ATGGCATCCATTGCGGCTTCGACGAAAACGGCGGGACATGCGCTGAACCGGCGCGCGGTGGCGGCGGCCGTGATCGGCAA
CGCGCTCGAGTGGTACGACTTCACCGTCTTCAGTTTCATGACGGTCGTGATCGCCCAATTGTTCTTTCCGACCAGCAACG
AATATTCGTCGCTGCTCCTCACGACCGCGACGTTCGGCGTCGCGTTCTTCATGCGGCCGATCGGCGGCATCGTGCTCGGC
CTCTATGCGGATCGCGCGGGACGCAAGGCGGCGTTGTCGCTCGTGATCCTGCTGATGACGCTCGGCATCTTCCTGATCGC
GGTCGCGCCGCCGTATGCGGCGATCGGCATCGGCGGGCCGCTTCTCATCGTGCTCGGCCGCCTGTTGCAGGGCTTCTCCG
CGGGCGGCGAATTCGGCAGCGCGACGGCGCTGCTGATCGAGGCCGCGCCGTTCTCGCGGCGCGGCTACTACGGCAGCTGG
CAGATGGCGAGCCAGGCGGCGGCGCTGCTGTTCGGCTCGCTCGTCGGCGCGGCGGTGACGCGCGGCCTGTCGACCGAGGC
ACTGCATGCGTGGGGCTGGCGCGTGCCGTTCCTGATCGGGCTCGTGATCGGGCCGGTCGGCTTCTATGTGCGCCGCCATC
TCGTCGATTCGGAAGCGTTCCTGCAGGCGCGCGAGCGCGGCGAGCGCGTGACGCTTCGCGAAGTGTTCGCGCGGCATGGG
CGTGCGGTGGTATGCGGGCTCGGCGCGGTGATCGCGCTCACCGTCACCGTCTACGTGCTGATCAGCTATCTGCCGACCTT
CGCCGTCAAGCAACTGAAGCTGCCTTACGGGCAATCGTTCTACGCGGTGATCATCGGCAACCTGCTGCTGACCGCGCTGT
CGCCCGTCGCGGGCGCATGGTCCGACCGGATCGGGCGCAAGGGGCTCTCGCTGTGGGCGCTCGGGTTCACGCTCGCGTTG
ATCTATCCGCTGTTCGAGTGGCTCGACGCGTCGCCGAGCATTGCGCGGCTGATCGTCGTGCAGGCGGTGCTGTCGGTGAC
GCTCGCCGGCTACTACGGGCCGTTCGGCGCGATGATCGCCGAGCTCTTTCCGGCGAACGTGCGCTCGACGGGGCTGTCGC
TCGCGTATAACGTCGCGGTGATGGTGTTCGGCGGCTTCGGCCAGTTCATCGTCACGTGGCTCATCAAGACCACGGGCACG
CCGCTTGCGCCGACCTATTACGTGATGGCGGGGCTCGCGCTGTCGCTCGTCGCGCTCGCGTTCGTGCCGGCGAGAAGCGG
CGATCTCGATTGA

Upstream 100 bases:

>100_bases
GCTCCACGGCGCGGCGTTCGCCTTTTTCGGCGACGCTATCCGTGCGCGACGCGCGATATACGACGACATCACGAACGCAC
GACGACAGCGAGGGGCGAGG

Downstream 100 bases:

>100_bases
TCGGCGCCGGCGGCCGCGCCATGCCGGCGGCCACGCCATCGCGCCGCGCTGCCGGCGCCGCGCGATGCGTGCGCCCGGCC
GGCCGCGCCGAGAAACATCA

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MASIAASTKTAGHALNRRAVAAAVIGNALEWYDFTVFSFMTVVIAQLFFPTSNEYSSLLLTTATFGVAFFMRPIGGIVLG
LYADRAGRKAALSLVILLMTLGIFLIAVAPPYAAIGIGGPLLIVLGRLLQGFSAGGEFGSATALLIEAAPFSRRGYYGSW
QMASQAAALLFGSLVGAAVTRGLSTEALHAWGWRVPFLIGLVIGPVGFYVRRHLVDSEAFLQARERGERVTLREVFARHG
RAVVCGLGAVIALTVTVYVLISYLPTFAVKQLKLPYGQSFYAVIIGNLLLTALSPVAGAWSDRIGRKGLSLWALGFTLAL
IYPLFEWLDASPSIARLIVVQAVLSVTLAGYYGPFGAMIAELFPANVRSTGLSLAYNVAVMVFGGFGQFIVTWLIKTTGT
PLAPTYYVMAGLALSLVALAFVPARSGDLD

Sequences:

>Translated_430_residues
MASIAASTKTAGHALNRRAVAAAVIGNALEWYDFTVFSFMTVVIAQLFFPTSNEYSSLLLTTATFGVAFFMRPIGGIVLG
LYADRAGRKAALSLVILLMTLGIFLIAVAPPYAAIGIGGPLLIVLGRLLQGFSAGGEFGSATALLIEAAPFSRRGYYGSW
QMASQAAALLFGSLVGAAVTRGLSTEALHAWGWRVPFLIGLVIGPVGFYVRRHLVDSEAFLQARERGERVTLREVFARHG
RAVVCGLGAVIALTVTVYVLISYLPTFAVKQLKLPYGQSFYAVIIGNLLLTALSPVAGAWSDRIGRKGLSLWALGFTLAL
IYPLFEWLDASPSIARLIVVQAVLSVTLAGYYGPFGAMIAELFPANVRSTGLSLAYNVAVMVFGGFGQFIVTWLIKTTGT
PLAPTYYVMAGLALSLVALAFVPARSGDLD
>Mature_429_residues
ASIAASTKTAGHALNRRAVAAAVIGNALEWYDFTVFSFMTVVIAQLFFPTSNEYSSLLLTTATFGVAFFMRPIGGIVLGL
YADRAGRKAALSLVILLMTLGIFLIAVAPPYAAIGIGGPLLIVLGRLLQGFSAGGEFGSATALLIEAAPFSRRGYYGSWQ
MASQAAALLFGSLVGAAVTRGLSTEALHAWGWRVPFLIGLVIGPVGFYVRRHLVDSEAFLQARERGERVTLREVFARHGR
AVVCGLGAVIALTVTVYVLISYLPTFAVKQLKLPYGQSFYAVIIGNLLLTALSPVAGAWSDRIGRKGLSLWALGFTLALI
YPLFEWLDASPSIARLIVVQAVLSVTLAGYYGPFGAMIAELFPANVRSTGLSLAYNVAVMVFGGFGQFIVTWLIKTTGTP
LAPTYYVMAGLALSLVALAFVPARSGDLD

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=406, Percent_Identity=31.0344827586207, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1788942, Length=413, Percent_Identity=33.8983050847458, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789941, Length=393, Percent_Identity=31.5521628498728, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1788292, Length=413, Percent_Identity=29.0556900726392, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI87082231, Length=213, Percent_Identity=27.6995305164319, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87082404, Length=373, Percent_Identity=23.3243967828418, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45680; Mature: 45549

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIAASTKTAGHALNRRAVAAAVIGNALEWYDFTVFSFMTVVIAQLFFPTSNEYSSLLL
CCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
TTATFGVAFFMRPIGGIVLGLYADRAGRKAALSLVILLMTLGIFLIAVAPPYAAIGIGGP
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHH
LLIVLGRLLQGFSAGGEFGSATALLIEAAPFSRRGYYGSWQMASQAAALLFGSLVGAAVT
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RGLSTEALHAWGWRVPFLIGLVIGPVGFYVRRHLVDSEAFLQARERGERVTLREVFARHG
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEHHHHHHHHCC
RAVVCGLGAVIALTVTVYVLISYLPTFAVKQLKLPYGQSFYAVIIGNLLLTALSPVAGAW
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SDRIGRKGLSLWALGFTLALIYPLFEWLDASPSIARLIVVQAVLSVTLAGYYGPFGAMIA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHH
ELFPANVRSTGLSLAYNVAVMVFGGFGQFIVTWLIKTTGTPLAPTYYVMAGLALSLVALA
HHCCCCHHHHCHHHHHHHHHHEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
FVPARSGDLD
HHCCCCCCCC
>Mature Secondary Structure 
ASIAASTKTAGHALNRRAVAAAVIGNALEWYDFTVFSFMTVVIAQLFFPTSNEYSSLLL
CCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
TTATFGVAFFMRPIGGIVLGLYADRAGRKAALSLVILLMTLGIFLIAVAPPYAAIGIGGP
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHH
LLIVLGRLLQGFSAGGEFGSATALLIEAAPFSRRGYYGSWQMASQAAALLFGSLVGAAVT
HHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RGLSTEALHAWGWRVPFLIGLVIGPVGFYVRRHLVDSEAFLQARERGERVTLREVFARHG
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEHHHHHHHHCC
RAVVCGLGAVIALTVTVYVLISYLPTFAVKQLKLPYGQSFYAVIIGNLLLTALSPVAGAW
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SDRIGRKGLSLWALGFTLALIYPLFEWLDASPSIARLIVVQAVLSVTLAGYYGPFGAMIA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHH
ELFPANVRSTGLSLAYNVAVMVFGGFGQFIVTWLIKTTGTPLAPTYYVMAGLALSLVALA
HHCCCCHHHHCHHHHHHHHHHEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
FVPARSGDLD
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]