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The map label for this gene is yafC [C]

Identifier: 83719170

GI number: 83719170

Start: 338809

End: 339771

Strand: Direct

Name: yafC [C]

Synonym: BTH_I0306

Alternate gene names: 83719170

Gene position: 338809-339771 (Clockwise)

Preceding gene: 83718650

Following gene: 83721076

Centisome position: 8.89

GC content: 68.74

Gene sequence:

>963_bases
ATGGATACCCTCGTCAGCATGAATGTGTTCCGCTATGTCGTCGAAGTCGGCAGCTTCGTCGGCGCGGCCGAGCGCATGGA
GATGTCGGCTGCGATGGCGAGCAAGCATGTGATGCATCTCGAGCATCAGCTCGGCGCGCGTCTCTTGCATCGCACGACGC
GCCGCGTCGCGCCGACGGAGGCGGGACGCGAATACTACGAGCGGCTCGTGCAGGCGCTCACCGAACTCGACGAGGCCGGG
CAGGCGGTGGGCGCCGCGAGCGTCGTGCCACAAGGGCGGCTGCGCGTGACGTCGCTGTCCGCGTTCGGGCTGCGGCACGT
GATGGACGCGGTGACGTCGTACGCGCAGCGCTACGCCGACGTGACGGTCGAGATCACGCTGTCCGACCGCGTCGTCGAGC
TGATCGACGAAGGCTACGACGTCGCGGTGCGCGCGGCGCCCGCCGGCATGAAGTCGTCGTCGCTCGTCGCGCGGCAGATC
GCGACCGCGCACATCCTGCTCGTCGCTTCGCCCGACTATCTCGCGAAGCACGGCACGCCGACGACGATCGCCGATCTTCA
GCATCACAGCTTCATTCGCCGCGAGCCGAACGAGACGCTGCTCGACGCGGCGATCTCCGACGTCGCGGCCGCCTCGCGCG
TGCCGCTGTCGGGCAACGTGATCGTCAATCATCTCGAAGGGCTGCGCGTCGCGGTGCTGGACGGCGCGGGCATCGCGCTC
CTCGGCACCGAAGTGGTGGGCGAGGACATCGAGTCCGGCCGTCTCGTGCCGTTGCTGCTCGATGCGCTGCCGCCGCGCGA
GCTGCCGATCTACGCGGTGTACGCGAGCCGCCGGCACGTATCGGCGAAGGTGCGCTCGTTCGTCGATTTCCTCGCCGATC
GCTTCGCCGGCCAGTCGCTGTGCCCGTCGATCGACGAGCGGTTGCGGCAGTTCGCGATGCCGCGGATGAAGCGGCTCGGA
TGA

Upstream 100 bases:

>100_bases
ATTAAACGGCGCCGTTTTGCCTTTTTTGACGTTTTCAATTCACCAGCTTTGTTACAGTTGCTTGCGATTGCATAATTCCC
CTCGATTGCGAGCATTTCGG

Downstream 100 bases:

>100_bases
TGCGCACGGGCGCCGCGTAGCGACGAAAAAAAAGCGGACCGTTCGGTCCGCTCCAGACTCAAGACTCAAGACGAACCCTC
GCGCGGCGAGGGTTTTGTCG

Product: LysR family regulatory protein

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MDTLVSMNVFRYVVEVGSFVGAAERMEMSAAMASKHVMHLEHQLGARLLHRTTRRVAPTEAGREYYERLVQALTELDEAG
QAVGAASVVPQGRLRVTSLSAFGLRHVMDAVTSYAQRYADVTVEITLSDRVVELIDEGYDVAVRAAPAGMKSSSLVARQI
ATAHILLVASPDYLAKHGTPTTIADLQHHSFIRREPNETLLDAAISDVAAASRVPLSGNVIVNHLEGLRVAVLDGAGIAL
LGTEVVGEDIESGRLVPLLLDALPPRELPIYAVYASRRHVSAKVRSFVDFLADRFAGQSLCPSIDERLRQFAMPRMKRLG

Sequences:

>Translated_320_residues
MDTLVSMNVFRYVVEVGSFVGAAERMEMSAAMASKHVMHLEHQLGARLLHRTTRRVAPTEAGREYYERLVQALTELDEAG
QAVGAASVVPQGRLRVTSLSAFGLRHVMDAVTSYAQRYADVTVEITLSDRVVELIDEGYDVAVRAAPAGMKSSSLVARQI
ATAHILLVASPDYLAKHGTPTTIADLQHHSFIRREPNETLLDAAISDVAAASRVPLSGNVIVNHLEGLRVAVLDGAGIAL
LGTEVVGEDIESGRLVPLLLDALPPRELPIYAVYASRRHVSAKVRSFVDFLADRFAGQSLCPSIDERLRQFAMPRMKRLG
>Mature_320_residues
MDTLVSMNVFRYVVEVGSFVGAAERMEMSAAMASKHVMHLEHQLGARLLHRTTRRVAPTEAGREYYERLVQALTELDEAG
QAVGAASVVPQGRLRVTSLSAFGLRHVMDAVTSYAQRYADVTVEITLSDRVVELIDEGYDVAVRAAPAGMKSSSLVARQI
ATAHILLVASPDYLAKHGTPTTIADLQHHSFIRREPNETLLDAAISDVAAASRVPLSGNVIVNHLEGLRVAVLDGAGIAL
LGTEVVGEDIESGRLVPLLLDALPPRELPIYAVYASRRHVSAKVRSFVDFLADRFAGQSLCPSIDERLRQFAMPRMKRLG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=32.6599326599327, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=34.3537414965986, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1789639, Length=298, Percent_Identity=30.5369127516779, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=28.5714285714286, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787128, Length=294, Percent_Identity=29.2517006802721, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1789440, Length=287, Percent_Identity=28.5714285714286, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787589, Length=298, Percent_Identity=27.5167785234899, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1789173, Length=269, Percent_Identity=26.7657992565056, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788706, Length=282, Percent_Identity=23.758865248227, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788481, Length=298, Percent_Identity=24.496644295302, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34816; Mature: 34816

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLVSMNVFRYVVEVGSFVGAAERMEMSAAMASKHVMHLEHQLGARLLHRTTRRVAPTE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGREYYERLVQALTELDEAGQAVGAASVVPQGRLRVTSLSAFGLRHVMDAVTSYAQRYAD
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHC
VTVEITLSDRVVELIDEGYDVAVRAAPAGMKSSSLVARQIATAHILLVASPDYLAKHGTP
EEEEEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHEEEEEECCHHHHHCCCC
TTIADLQHHSFIRREPNETLLDAAISDVAAASRVPLSGNVIVNHLEGLRVAVLDGAGIAL
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHEECCCCCEEEEECCCCEEE
LGTEVVGEDIESGRLVPLLLDALPPRELPIYAVYASRRHVSAKVRSFVDFLADRFAGQSL
EEHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCC
CPSIDERLRQFAMPRMKRLG
CHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDTLVSMNVFRYVVEVGSFVGAAERMEMSAAMASKHVMHLEHQLGARLLHRTTRRVAPTE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGREYYERLVQALTELDEAGQAVGAASVVPQGRLRVTSLSAFGLRHVMDAVTSYAQRYAD
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHC
VTVEITLSDRVVELIDEGYDVAVRAAPAGMKSSSLVARQIATAHILLVASPDYLAKHGTP
EEEEEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHEEEEEECCHHHHHCCCC
TTIADLQHHSFIRREPNETLLDAAISDVAAASRVPLSGNVIVNHLEGLRVAVLDGAGIAL
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHEECCCCCEEEEECCCCEEE
LGTEVVGEDIESGRLVPLLLDALPPRELPIYAVYASRRHVSAKVRSFVDFLADRFAGQSL
EEHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCC
CPSIDERLRQFAMPRMKRLG
CHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]