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The map label for this gene is pcm

Identifier: 83719117

GI number: 83719117

Start: 2500849

End: 2501832

Strand: Direct

Name: pcm

Synonym: BTH_I2224

Alternate gene names: 83719117

Gene position: 2500849-2501832 (Clockwise)

Preceding gene: 83719865

Following gene: 83721288

Centisome position: 65.65

GC content: 72.36

Gene sequence:

>984_bases
ATGAGCGGCGAGCGCGCGAAGCGGTTTCCGCTCGCGCTCGAGGATCTGAAGCGCGAGCCGCGCAAGCCCGAAGGGCGCGC
GGCCGAGCGTCAGGCCGCGGGCGACGCCGCCCGTCAGCGCCTCATCGCCGCGGCGGCGGCCGCGCCCGCCGCCGCCTCCC
CGATCGCGCCCGAACGCCTCGCGCCGCGCGCGGCCGGCGCGTCGGGGAGCGGCGTGCCCGTTGCGAAACCGGCGCGTGCG
AAACCGCCCACGACGCCCGGCGTCGCAAAGAGCGCGCCGTCCTCCGGCGTGAAGAACGGCGACAAGAGCGCGACGCCGAA
CGTCGCGTTGAGCGGCGCGCTCGCGCTGACTTCGGAACGGGTGCGCGAGCGCATGGTCGAACGGCTTCGGGCAAACGGCG
TGGCCGATCCGCGCGTGCTCGCCGCGATGTCGGCCGTGCCGCGCCACATGTTCGTGGATCCGGGGCTTGCGGCCCAGGCG
TACGAGGACGCCGCGCTGCCGATCGGCCATCAGCAGACGATCTCGAAGCCGTCGGTCGTCGCGCGGATGATCGAGCTCGC
GGCGGCGGGCCGCACGCTCGAGCGCGTGCTCGAAATCGGCACGGGCTGCGGCTATCAGGCCGCGGTGCTGAGTCGCGTCG
CGCGCGACGTGTATTCGATCGAACGCGTGAAGCCGCTCTACGAGCGCGCGAAACTGAATTTGCGGCCGCTGCGCGTGCCG
AACATCCGCCTGCACTACGGCGACGGACGCGTGGGCCTGCCCGCCGCGGCGCCGTTCGACGCGATCGTGATCGCCGCGGC
GGGGCTCGACGTGCCGCGGGCGCTGTTGGAACAATTGGCGATCGGCGGCAGGCTCGTCGCGCCTGTCGGAGAACAGGCGG
GCGAGCAGGTGCTCACGCTCGTGGAGCGCGTCGCACCCGCGCAGTGGCGTGAGTCGCGGCTTGATCGCGTTTTCTTTGTC
CCTTTAAAATCCGGAGTGATTTGA

Upstream 100 bases:

>100_bases
CCGCGGGCTACGTGTCGATCACGCCGCTGCAGCTCGATCTCACCCACACGCAAATGCTGGCGGCCACGCGCGATTGGGCG
CATGCCGGGAGCGGCGCTTC

Downstream 100 bases:

>100_bases
TACCGATGAGTATGCTGCGCGCGATGCAAAACAATCGTTCGAAGGTTCCGCTCTCGCTCGCTCAGCGCGCGATTTGCATG
GCGGCGCTCACGCTGCTCGC

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSGERAKRFPLALEDLKREPRKPEGRAAERQAAGDAARQRLIAAAAAAPAAASPIAPERLAPRAAGASGSGVPVAKPARA
KPPTTPGVAKSAPSSGVKNGDKSATPNVALSGALALTSERVRERMVERLRANGVADPRVLAAMSAVPRHMFVDPGLAAQA
YEDAALPIGHQQTISKPSVVARMIELAAAGRTLERVLEIGTGCGYQAAVLSRVARDVYSIERVKPLYERAKLNLRPLRVP
NIRLHYGDGRVGLPAAAPFDAIVIAAAGLDVPRALLEQLAIGGRLVAPVGEQAGEQVLTLVERVAPAQWRESRLDRVFFV
PLKSGVI

Sequences:

>Translated_327_residues
MSGERAKRFPLALEDLKREPRKPEGRAAERQAAGDAARQRLIAAAAAAPAAASPIAPERLAPRAAGASGSGVPVAKPARA
KPPTTPGVAKSAPSSGVKNGDKSATPNVALSGALALTSERVRERMVERLRANGVADPRVLAAMSAVPRHMFVDPGLAAQA
YEDAALPIGHQQTISKPSVVARMIELAAAGRTLERVLEIGTGCGYQAAVLSRVARDVYSIERVKPLYERAKLNLRPLRVP
NIRLHYGDGRVGLPAAAPFDAIVIAAAGLDVPRALLEQLAIGGRLVAPVGEQAGEQVLTLVERVAPAQWRESRLDRVFFV
PLKSGVI
>Mature_326_residues
SGERAKRFPLALEDLKREPRKPEGRAAERQAAGDAARQRLIAAAAAAPAAASPIAPERLAPRAAGASGSGVPVAKPARAK
PPTTPGVAKSAPSSGVKNGDKSATPNVALSGALALTSERVRERMVERLRANGVADPRVLAAMSAVPRHMFVDPGLAAQAY
EDAALPIGHQQTISKPSVVARMIELAAAGRTLERVLEIGTGCGYQAAVLSRVARDVYSIERVKPLYERAKLNLRPLRVPN
IRLHYGDGRVGLPAAAPFDAIVIAAAGLDVPRALLEQLAIGGRLVAPVGEQAGEQVLTLVERVAPAQWRESRLDRVFFVP
LKSGVI

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=211, Percent_Identity=31.7535545023697, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1789100, Length=210, Percent_Identity=44.7619047619048, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71983477, Length=219, Percent_Identity=32.4200913242009, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI193207222, Length=218, Percent_Identity=32.1100917431193, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17981723, Length=216, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_BURTA (Q2SWF4)

Other databases:

- EMBL:   CP000086
- RefSeq:   YP_442745.1
- ProteinModelPortal:   Q2SWF4
- SMR:   Q2SWF4
- STRING:   Q2SWF4
- GeneID:   3846874
- GenomeReviews:   CP000086_GR
- KEGG:   bte:BTH_I2224
- TIGR:   BTH_I2224
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   HEQAKAN
- ProtClustDB:   CLSK896871
- BioCyc:   BTHA271848:BTH_I2224-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 34370; Mature: 34239

Theoretical pI: Translated: 11.11; Mature: 11.11

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGERAKRFPLALEDLKREPRKPEGRAAERQAAGDAARQRLIAAAAAAPAAASPIAPERL
CCCCCCHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
APRAAGASGSGVPVAKPARAKPPTTPGVAKSAPSSGVKNGDKSATPNVALSGALALTSER
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHH
VRERMVERLRANGVADPRVLAAMSAVPRHMFVDPGLAAQAYEDAALPIGHQQTISKPSVV
HHHHHHHHHHHCCCCCHHHHHHHHHHCHHHCCCCCCHHHHHHCCCCCCCCHHHCCCHHHH
ARMIELAAAGRTLERVLEIGTGCGYQAAVLSRVARDVYSIERVKPLYERAKLNLRPLRVP
HHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECC
NIRLHYGDGRVGLPAAAPFDAIVIAAAGLDVPRALLEQLAIGGRLVAPVGEQAGEQVLTL
CEEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHH
VERVAPAQWRESRLDRVFFVPLKSGVI
HHHHCCHHHHHHHCCEEEEEECCCCCC
>Mature Secondary Structure 
SGERAKRFPLALEDLKREPRKPEGRAAERQAAGDAARQRLIAAAAAAPAAASPIAPERL
CCCCCHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
APRAAGASGSGVPVAKPARAKPPTTPGVAKSAPSSGVKNGDKSATPNVALSGALALTSER
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHH
VRERMVERLRANGVADPRVLAAMSAVPRHMFVDPGLAAQAYEDAALPIGHQQTISKPSVV
HHHHHHHHHHHCCCCCHHHHHHHHHHCHHHCCCCCCHHHHHHCCCCCCCCHHHCCCHHHH
ARMIELAAAGRTLERVLEIGTGCGYQAAVLSRVARDVYSIERVKPLYERAKLNLRPLRVP
HHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECC
NIRLHYGDGRVGLPAAAPFDAIVIAAAGLDVPRALLEQLAIGGRLVAPVGEQAGEQVLTL
CEEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCEEECCCCHHHHHHHHHH
VERVAPAQWRESRLDRVFFVPLKSGVI
HHHHCCHHHHHHHCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA