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The map label for this gene is pucL [H]

Identifier: 83718857

GI number: 83718857

Start: 2341688

End: 2342212

Strand: Direct

Name: pucL [H]

Synonym: BTH_I2068

Alternate gene names: 83718857

Gene position: 2341688-2342212 (Clockwise)

Preceding gene: 83720732

Following gene: 161723159

Centisome position: 61.47

GC content: 66.86

Gene sequence:

>525_bases
ATGATGAAGGCGATGCGCTACACGCTCGAACAATTGAACGCGATGTCGACGGAGGCGTTCGTCGCGGCGCTGTCCGGGAT
CTTCGAGCATTCGCCGTGGGTCGCCGAGGCGGCTGCGGCCGCGCGGCCCTTCAGCAGCATCGACGCATTGCATCGGACGA
TGCGGGACGCGGTCGACGCCGCGGGCGACGCGCGGCAGCTCGCGCTCATCAATGCGCACCCGGAGCTCGCCGGCAAGGCG
GCGGTGCGCGGTGAGCTGACCGCGGAATCGACGCGCGAGCAGAGCGGCGCGGGCCTCGATCGCTGCACGCAGGAGGAGTT
CGACAAGCTGCAGCGGCTCAACCGCGCGTATCGCGAGAAATTCGGCTTCCCGTTCATTCTTGCGGTGCGCGGCTATGACC
GGCACGGAATCATCGAGAACTTCGAGGCGCGGATCGACCATACGCAGGACGAGGAGTTGCGCGCGAGCCTCGGGCAGATC
TATCGGATCGCGCGTTTCCGGCTCGACGATCTGATCGACGCGTGA

Upstream 100 bases:

>100_bases
AGCGGCACGATCGCGTATGGGTCGCGCGTCGCGTCGATATCGCGCGGCACTGGCGCGAGCATCATCCGTATCGGCCGCAC
GCTCGACGCGAGGGGGACGC

Downstream 100 bases:

>100_bases
GCGTCGATTGAGCGCCCCGGCTGGTTGGGCCCGGCGCAGCGCGGCGTTGCATCGCGCTGCGATCGGCCAATTCCGGCGAC
GCGGAAACCAATGATGCGAT

Product: hypothetical protein

Products: NA

Alternate protein names: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase; OHCU decarboxylase; Uricase; Urate oxidase [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MMKAMRYTLEQLNAMSTEAFVAALSGIFEHSPWVAEAAAAARPFSSIDALHRTMRDAVDAAGDARQLALINAHPELAGKA
AVRGELTAESTREQSGAGLDRCTQEEFDKLQRLNRAYREKFGFPFILAVRGYDRHGIIENFEARIDHTQDEELRASLGQI
YRIARFRLDDLIDA

Sequences:

>Translated_174_residues
MMKAMRYTLEQLNAMSTEAFVAALSGIFEHSPWVAEAAAAARPFSSIDALHRTMRDAVDAAGDARQLALINAHPELAGKA
AVRGELTAESTREQSGAGLDRCTQEEFDKLQRLNRAYREKFGFPFILAVRGYDRHGIIENFEARIDHTQDEELRASLGQI
YRIARFRLDDLIDA
>Mature_174_residues
MMKAMRYTLEQLNAMSTEAFVAALSGIFEHSPWVAEAAAAARPFSSIDALHRTMRDAVDAAGDARQLALINAHPELAGKA
AVRGELTAESTREQSGAGLDRCTQEEFDKLQRLNRAYREKFGFPFILAVRGYDRHGIIENFEARIDHTQDEELRASLGQI
YRIARFRLDDLIDA

Specific function: Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5- ureidoimidazoline (OHCU) to (S)-allantoin [H]

COG id: COG3195

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the uricase family [H]

Homologues:

Organism=Homo sapiens, GI157743280, Length=166, Percent_Identity=39.1566265060241, Blast_Score=117, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018020
- InterPro:   IPR017580
- InterPro:   IPR002042
- InterPro:   IPR019842 [H]

Pfam domain/function: PF09349 OHCU_decarbox; PF01014 Uricase [H]

EC number: =1.7.3.3 [H]

Molecular weight: Translated: 19484; Mature: 19484

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKAMRYTLEQLNAMSTEAFVAALSGIFEHSPWVAEAAAAARPFSSIDALHRTMRDAVDA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHC
AGDARQLALINAHPELAGKAAVRGELTAESTREQSGAGLDRCTQEEFDKLQRLNRAYREK
CCCCHHEEEEECCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
FGFPFILAVRGYDRHGIIENFEARIDHTQDEELRASLGQIYRIARFRLDDLIDA
CCCCEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMKAMRYTLEQLNAMSTEAFVAALSGIFEHSPWVAEAAAAARPFSSIDALHRTMRDAVDA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHC
AGDARQLALINAHPELAGKAAVRGELTAESTREQSGAGLDRCTQEEFDKLQRLNRAYREK
CCCCHHEEEEECCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
FGFPFILAVRGYDRHGIIENFEARIDHTQDEELRASLGQIYRIARFRLDDLIDA
CCCCEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]