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The map label for this gene is braE [H]

Identifier: 83718822

GI number: 83718822

Start: 903249

End: 904418

Strand: Direct

Name: braE [H]

Synonym: BTH_I0786

Alternate gene names: 83718822

Gene position: 903249-904418 (Clockwise)

Preceding gene: 83719719

Following gene: 83721605

Centisome position: 23.71

GC content: 65.98

Gene sequence:

>1170_bases
ATGACATCCATTCAACCGATCGAAACGTCGGCTTCGCTCGTCGAGGAGCGCCATCCGGCGAAGACCGCCACCATCAGCAT
CCTGATCGCGGCGTTCGTGATCGCCGCGCCGCTCGTCATCGGCGCGGCGGGCGGCAACTACTGGGTCCGCGTGCTCGACT
TCGCGATGCTGTACGTGATGCTCGCGCTGGGCTTGAACGTGGTCGTCGGCTTCGCCGGCCTGCTGGACCTCGGCTACATC
GCGTTCTACGCGGTGGGCGCGTACACCGCGGCGCTCCTGAGCTCGCCGCACCTGACGTCGAACTTCGACTGGATCGCGCA
CGCGTTTCCAGCCGGCCTGCACGTGCCGTTCTTCCTGATCGTGCCGATCGCGATGGCGCTCGCCGCGGTCTTCGGCATCC
TGCTCGGCGCGCCGACGCTGCGCCTGCGCGGCGACTACCTCGCGATCGTCACGCTCGGCTTCGGCGAGATCGTCCGAATC
TTCATGAACAACCTCGACCGTCCGGTCAACGTGACGAACGGGCCGCAGGGCATCACCGGCATCGATCCCGTCACGATCGC
GGGGTTCAACCTGTCGCAGACGCACCAGATCTTCGGATTTTCGCTGCCGTCGGTGTACATGTACTACTACCTGTTCGTGC
TTTGCGCGCTGCTCGTCATCTGGGTCTGCACGCGCCTGCAGCACTCGCGGATCGGCCGCGCATGGGCCGCGATCCGCGAA
GATGAAATCGCCGCCAAGGCGATGGGCATCAACACCCGCAACGTGAAGCTGCTCGCGTTCGCGATGGGCGCGTCGTTCGG
CGGCCTGTCGGGCGCGATGTTCGGCGCGTTCCAGGGCTTCGTGTCGCCGGAATCGTTCACGTTCTGGGAGTCGGTCGTCG
TGCTCGCGTGCGTCGTGCTGGGCGGCATGGGCCACATCCCGGGCGTGATCCTCGGCGCGGTCCTGCTCGCCGTGTTCCCC
GAGTTCCTGCGCTCGACGATGAGCCCGCTGCAGCACATGCTGTTCGGCCACGACATCGTCGATACCGAGGTGATTCGCCA
GGCCCTTTACGGCCTCGCGATGGTGGTCATCATGCTGTATCGCTCGGAAGGCCTGTGGCCCGCGCCGAAGCATGAGGACA
GGATCGCGAAACTGGCGAAGCGCGGCGGCAAGAAGCCGGTGCGCGCGTAA

Upstream 100 bases:

>100_bases
CAGGACGTGTTCGCGTTCATCGTGCTCATCATCGTGCTCGTGTTCCGGCCGTCGGGCCTGTTGGGCGAACGTGTCGCCGA
TCGCGCGTAAGGAGAACAAC

Downstream 100 bases:

>100_bases
AGGACGTGGGACCGGCGCCCAGCGCCAGCGCGCGAGCGCCGGTCGACACAGGAGAAAAGGTAATGAGCGAACAAATTCGA
CTGTCCGTCAAAGGCGTGAA

Product: branched-chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTSIQPIETSASLVEERHPAKTATISILIAAFVIAAPLVIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYTAALLSSPHLTSNFDWIAHAFPAGLHVPFFLIVPIAMALAAVFGILLGAPTLRLRGDYLAIVTLGFGEIVRI
FMNNLDRPVNVTNGPQGITGIDPVTIAGFNLSQTHQIFGFSLPSVYMYYYLFVLCALLVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTFWESVVVLACVVLGGMGHIPGVILGAVLLAVFP
EFLRSTMSPLQHMLFGHDIVDTEVIRQALYGLAMVVIMLYRSEGLWPAPKHEDRIAKLAKRGGKKPVRA

Sequences:

>Translated_389_residues
MTSIQPIETSASLVEERHPAKTATISILIAAFVIAAPLVIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYTAALLSSPHLTSNFDWIAHAFPAGLHVPFFLIVPIAMALAAVFGILLGAPTLRLRGDYLAIVTLGFGEIVRI
FMNNLDRPVNVTNGPQGITGIDPVTIAGFNLSQTHQIFGFSLPSVYMYYYLFVLCALLVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTFWESVVVLACVVLGGMGHIPGVILGAVLLAVFP
EFLRSTMSPLQHMLFGHDIVDTEVIRQALYGLAMVVIMLYRSEGLWPAPKHEDRIAKLAKRGGKKPVRA
>Mature_388_residues
TSIQPIETSASLVEERHPAKTATISILIAAFVIAAPLVIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYIA
FYAVGAYTAALLSSPHLTSNFDWIAHAFPAGLHVPFFLIVPIAMALAAVFGILLGAPTLRLRGDYLAIVTLGFGEIVRIF
MNNLDRPVNVTNGPQGITGIDPVTIAGFNLSQTHQIFGFSLPSVYMYYYLFVLCALLVIWVCTRLQHSRIGRAWAAIRED
EIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTFWESVVVLACVVLGGMGHIPGVILGAVLLAVFPE
FLRSTMSPLQHMLFGHDIVDTEVIRQALYGLAMVVIMLYRSEGLWPAPKHEDRIAKLAKRGGKKPVRA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=346, Percent_Identity=43.3526011560694, Blast_Score=217, Evalue=8e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 41960; Mature: 41829

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIQPIETSASLVEERHPAKTATISILIAAFVIAAPLVIGAAGGNYWVRVLDFAMLYVM
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LALGLNVVVGFAGLLDLGYIAFYAVGAYTAALLSSPHLTSNFDWIAHAFPAGLHVPFFLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHH
VPIAMALAAVFGILLGAPTLRLRGDYLAIVTLGFGEIVRIFMNNLDRPVNVTNGPQGITG
HHHHHHHHHHHHHHHCCCCEEECCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
IDPVTIAGFNLSQTHQIFGFSLPSVYMYYYLFVLCALLVIWVCTRLQHSRIGRAWAAIRE
CCCCEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTFWESVVVLACVVL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GGMGHIPGVILGAVLLAVFPEFLRSTMSPLQHMLFGHDIVDTEVIRQALYGLAMVVIMLY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RSEGLWPAPKHEDRIAKLAKRGGKKPVRA
HCCCCCCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TSIQPIETSASLVEERHPAKTATISILIAAFVIAAPLVIGAAGGNYWVRVLDFAMLYVM
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LALGLNVVVGFAGLLDLGYIAFYAVGAYTAALLSSPHLTSNFDWIAHAFPAGLHVPFFLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHH
VPIAMALAAVFGILLGAPTLRLRGDYLAIVTLGFGEIVRIFMNNLDRPVNVTNGPQGITG
HHHHHHHHHHHHHHHCCCCEEECCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
IDPVTIAGFNLSQTHQIFGFSLPSVYMYYYLFVLCALLVIWVCTRLQHSRIGRAWAAIRE
CCCCEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTFWESVVVLACVVL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GGMGHIPGVILGAVLLAVFPEFLRSTMSPLQHMLFGHDIVDTEVIRQALYGLAMVVIMLY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RSEGLWPAPKHEDRIAKLAKRGGKKPVRA
HCCCCCCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]