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The map label for this gene is trmB

Identifier: 83718399

GI number: 83718399

Start: 1711278

End: 1712075

Strand: Reverse

Name: trmB

Synonym: BTH_I1513

Alternate gene names: 83718399

Gene position: 1712075-1711278 (Counterclockwise)

Preceding gene: 83720429

Following gene: 161723176

Centisome position: 44.95

GC content: 70.18

Gene sequence:

>798_bases
ATGAACCACGACGACCCGAACGCCTCCGGCGTGCCGCACGACGACGCCAATGACGCCGCCCCCGCTTCGGCAAGCGACGC
CGCTCGAGCCACCGGCCACGCCGACGACGAATCGAGCCCGCTTCATCTGCGCCGCATCCGCAGCTTCGTCACGCGCGCCG
GCCGCGTATCCACCGGCCAGCGCCGCGCGATCGACGAGCTCGGCCCGCGCTTCGTCGTCCCGTACGACAATGCCCAGCCG
GACTGGGACACGGTCTTCGGCCGCCGCGCGCCGCGCGTGCTCGAGATCGGCTTCGGGATGGGCGCGTCGACGGCCGAGAT
CGCCGCGCATCGCCCGGGCGACGACTTCATCGGCGTCGAGGTGCACGAGCCGGGCGTCGGCGCGCTGCTGAAACTGATCG
GCGAGCAGCAACTGTCGAACATCCGCATCATCCAGCACGATGCGGTCGAGGTGCTCGAGCACATGATCGCGCCGGACAGC
CTCGACGGCGCGCACATCTTCTTCCCGGACCCGTGGCACAAGGCGCGCCATCACAAGCGCCGGCTGATCCAGCCGCCGTT
CGTCGCGCAGCTTGCCGCGCGGCTCAAGCCGGGCGCGTACCTGCATTGCGCGACCGACTGGCAGAACTATGCGGAGCAGA
TGCTCGAGGTGCTGGGCGCGGACCCGTCGCTCGAAAACACCGCGCAAGACTATGCGCCCCGCCCCGACTATCGCCCGGTG
ACGAAGTTCGAGCGCCGCGGGCTGCGGCTCGGGCACGGCGTATGGGATCTCGTGTTCAGGAAGAAGCGCGCGGGCTGA

Upstream 100 bases:

>100_bases
GCTTTTTCGTATTTCATACGCAGTAAGGGCGGCCCGTCCAGCACGACGCCGTCGCGCGCCGCGCATCCGGCCCGCCCCTC
GCCTTTCATTCATTGTCACG

Downstream 100 bases:

>100_bases
CGCGCGACGCCGCGCCGACTGCGCGCGGGGTCGGCCGGACTGCGCCCGGGCGCATCGAAGGTTCGAATTGAAGGTTCGAC
ACGGCGGCGCGCGCGCCGCC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MNHDDPNASGVPHDDANDAAPASASDAARATGHADDESSPLHLRRIRSFVTRAGRVSTGQRRAIDELGPRFVVPYDNAQP
DWDTVFGRRAPRVLEIGFGMGASTAEIAAHRPGDDFIGVEVHEPGVGALLKLIGEQQLSNIRIIQHDAVEVLEHMIAPDS
LDGAHIFFPDPWHKARHHKRRLIQPPFVAQLAARLKPGAYLHCATDWQNYAEQMLEVLGADPSLENTAQDYAPRPDYRPV
TKFERRGLRLGHGVWDLVFRKKRAG

Sequences:

>Translated_265_residues
MNHDDPNASGVPHDDANDAAPASASDAARATGHADDESSPLHLRRIRSFVTRAGRVSTGQRRAIDELGPRFVVPYDNAQP
DWDTVFGRRAPRVLEIGFGMGASTAEIAAHRPGDDFIGVEVHEPGVGALLKLIGEQQLSNIRIIQHDAVEVLEHMIAPDS
LDGAHIFFPDPWHKARHHKRRLIQPPFVAQLAARLKPGAYLHCATDWQNYAEQMLEVLGADPSLENTAQDYAPRPDYRPV
TKFERRGLRLGHGVWDLVFRKKRAG
>Mature_265_residues
MNHDDPNASGVPHDDANDAAPASASDAARATGHADDESSPLHLRRIRSFVTRAGRVSTGQRRAIDELGPRFVVPYDNAQP
DWDTVFGRRAPRVLEIGFGMGASTAEIAAHRPGDDFIGVEVHEPGVGALLKLIGEQQLSNIRIIQHDAVEVLEHMIAPDS
LDGAHIFFPDPWHKARHHKRRLIQPPFVAQLAARLKPGAYLHCATDWQNYAEQMLEVLGADPSLENTAQDYAPRPDYRPV
TKFERRGLRLGHGVWDLVFRKKRAG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=223, Percent_Identity=53.3632286995516, Blast_Score=243, Evalue=7e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_BURTA (Q2SYE0)

Other databases:

- EMBL:   CP000086
- RefSeq:   YP_442059.1
- ProteinModelPortal:   Q2SYE0
- SMR:   Q2SYE0
- STRING:   Q2SYE0
- GeneID:   3846934
- GenomeReviews:   CP000086_GR
- KEGG:   bte:BTH_I1513
- TIGR:   BTH_I1513
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- ProtClustDB:   PRK00121
- BioCyc:   BTHA271848:BTH_I1513-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 29365; Mature: 29365

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: BINDING 95-95 BINDING 120-120 BINDING 147-147 BINDING 170-170 BINDING 174-174 BINDING 206-206

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHDDPNASGVPHDDANDAAPASASDAARATGHADDESSPLHLRRIRSFVTRAGRVSTGQ
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCH
RRAIDELGPRFVVPYDNAQPDWDTVFGRRAPRVLEIGFGMGASTAEIAAHRPGDDFIGVE
HHHHHHHCCEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCCCEEEEE
VHEPGVGALLKLIGEQQLSNIRIIQHDAVEVLEHMIAPDSLDGAHIFFPDPWHKARHHKR
ECCCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHH
RLIQPPFVAQLAARLKPGAYLHCATDWQNYAEQMLEVLGADPSLENTAQDYAPRPDYRPV
HCCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCH
TKFERRGLRLGHGVWDLVFRKKRAG
HHHHHCCCHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNHDDPNASGVPHDDANDAAPASASDAARATGHADDESSPLHLRRIRSFVTRAGRVSTGQ
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCH
RRAIDELGPRFVVPYDNAQPDWDTVFGRRAPRVLEIGFGMGASTAEIAAHRPGDDFIGVE
HHHHHHHCCEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCCCEEEEE
VHEPGVGALLKLIGEQQLSNIRIIQHDAVEVLEHMIAPDSLDGAHIFFPDPWHKARHHKR
ECCCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHH
RLIQPPFVAQLAARLKPGAYLHCATDWQNYAEQMLEVLGADPSLENTAQDYAPRPDYRPV
HCCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCH
TKFERRGLRLGHGVWDLVFRKKRAG
HHHHHCCCHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA