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The map label for this gene is 83595116

Identifier: 83595116

GI number: 83595116

Start: 4337976

End: 4339373

Strand: Direct

Name: 83595116

Synonym: Rru_A3787

Alternate gene names: NA

Gene position: 4337976-4339373 (Clockwise)

Preceding gene: 83595115

Following gene: 83595117

Centisome position: 99.66

GC content: 66.09

Gene sequence:

>1398_bases
GTGAAAGATATCAAACCGCTCCTGATATCGGGCAAGGAAGTGTATCCCCTGGTCGAAGGCGGCAAGGGCGTTGCGGTGAC
GAACGGCCGCAGCTCGGGCGCCTGGGCGGCCGCTGGTGGTGTTGGCACCATCAGCGCGGTGAATGCCGATTTTTATGACG
AGACCGGCGCCCTGGCCAATCAGGTTTACAAGGGGCGGACGCGCAGCGAACGCCACCGCGAGCTGATCGACTTCGGCATC
AAGGGCGGCATCGTCCAGGCCCGCATCGCCCACGAGGAAGCGCGCGGCGAAGGGCGGATGCACATCAACGTCCTGTGGGA
AATGGGCGGGGCCCGCGAGGTTCTCGAAGGCGTGCTCGAAGGCGCCAAGGGCCTGGTCCATGGCGTGACCTGCGGCGCCG
GCATGCCCTATGGCGTGGCCGAGATCGCCGCCCGCCACCGCGTCTATTACTATCCCATCGTGTCGTCGGCCCGCGCCTTC
CGCGCCCTGTGGAAGCGGGCCTATAGCAAGGCCCCCGAATGGCTGGGCGGCGTCGTCTATGAGGATCCCTGGCTGGCCGG
CGGCCACAATGGCCTGTCCAACAGCGAAAACCCGCGCGAACCCCAGCCGCCCCTGCCGCGCGTCGCCGAATTGCGCGCCC
AGATGCGCGCCGTCGGCGCTCCCGAGGTGCCGATCATCATGGCCGGCGGCGTGTGGTATCTGCGCGAATGGGCCGAATGG
CTTGAAAACCCCGAGCTGGGGCCGATCGCCTTCCAGTTCGGCACCCGGCCGCTGCTGACCCAGGAAAGCCCGATTTCCGA
CGAGTGGAAGCAGCGCCTGCTGACCTTGCGTCCGGGCGACGTGTTGCTCCATCGCTTCAGCCCGACGGGGTTCTACTCCT
CGGCCGTGCGCAATGACTTCCTTCAGGAACTGGTCGAGCGCTCCAACCGCCAGATCACCTATTTCACCGAGCCCCAGGGC
CAGCACACCACCAGCTTCGCCGTCGGCCCGCGCGCCCGCGAGGTGTTCGTGCGCGCCGAGGATAGCGTTCTGGCCCATGC
CTGGGTCGCCCAGGGCTTCACCGAAGCCATGCGCACGCCCGATAACTCGCTGATCTTCGTCACGCCCGAGCGCGCCGAGC
GCATCAAGACCGACCAGATCAATTGCATGGGCTGCCTGTCGGCCTGCGGGTTCTCGAACTGGGCGGAGAACGAGTTGAAC
AACACCGGCAAGCGCGCCGACCCCCGCTCGTTCTGTATTCAAAAGACCCTCCAGGAAATCGCCCACGGCCACCCCGTCGA
CCAGAACCTGATGTTCGCCGGCCATAACGCCTTCCGCTTCGCCACCGATCCGTTCTTCACTTCGGGACGGATTCCGACCA
TGGGCGAGTTGGTCGAGCGCATCCTGACGGGCGATTGA

Upstream 100 bases:

>100_bases
GGCCGGTGATGCCGGCGCGGTGCGGAGTGCGGAGATTTTGCCGTTTTGATTTCCATGACGGATCCATGGACAAGAAAAGC
AGACGGGAAGGGCAGCGTTC

Downstream 100 bases:

>100_bases
CCGGCAACCGAAAAGGGTTTTGCAGCCTGGAATTTAGTTCCTAGCTAGAACGGATATTGACTTAGTTCCAATTTGCCGTG
AGTCTCTGCTATGGTTAGGG

Product: 2-nitropropane dioxygenase, NPD

Products: acetone; nitrite

Alternate protein names: 2-Nitropropane Dioxygenase; 2-Nitropropane Dioxygenase Family Oxidoreductase; Oxidoreductase 2-Nitropropane Dioxygenase Family; Oxidoreductase; 2-Nitropropane Dioxygenase-Like Protein; Oxidoreductase 2-Nitropropane Dioxygenase Family Protein; Dioxygenase; 2-Nitropropane Dioxygenase Npd; Flavin-Containing Oxidoreductase; 2-Nitropropane Dioxygenase Family; 2-Nitropropane Dioxygenase Family Protein; 2-Nitropropane Dioxygenase-Like Dioxygenase

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MKDIKPLLISGKEVYPLVEGGKGVAVTNGRSSGAWAAAGGVGTISAVNADFYDETGALANQVYKGRTRSERHRELIDFGI
KGGIVQARIAHEEARGEGRMHINVLWEMGGAREVLEGVLEGAKGLVHGVTCGAGMPYGVAEIAARHRVYYYPIVSSARAF
RALWKRAYSKAPEWLGGVVYEDPWLAGGHNGLSNSENPREPQPPLPRVAELRAQMRAVGAPEVPIIMAGGVWYLREWAEW
LENPELGPIAFQFGTRPLLTQESPISDEWKQRLLTLRPGDVLLHRFSPTGFYSSAVRNDFLQELVERSNRQITYFTEPQG
QHTTSFAVGPRAREVFVRAEDSVLAHAWVAQGFTEAMRTPDNSLIFVTPERAERIKTDQINCMGCLSACGFSNWAENELN
NTGKRADPRSFCIQKTLQEIAHGHPVDQNLMFAGHNAFRFATDPFFTSGRIPTMGELVERILTGD

Sequences:

>Translated_465_residues
MKDIKPLLISGKEVYPLVEGGKGVAVTNGRSSGAWAAAGGVGTISAVNADFYDETGALANQVYKGRTRSERHRELIDFGI
KGGIVQARIAHEEARGEGRMHINVLWEMGGAREVLEGVLEGAKGLVHGVTCGAGMPYGVAEIAARHRVYYYPIVSSARAF
RALWKRAYSKAPEWLGGVVYEDPWLAGGHNGLSNSENPREPQPPLPRVAELRAQMRAVGAPEVPIIMAGGVWYLREWAEW
LENPELGPIAFQFGTRPLLTQESPISDEWKQRLLTLRPGDVLLHRFSPTGFYSSAVRNDFLQELVERSNRQITYFTEPQG
QHTTSFAVGPRAREVFVRAEDSVLAHAWVAQGFTEAMRTPDNSLIFVTPERAERIKTDQINCMGCLSACGFSNWAENELN
NTGKRADPRSFCIQKTLQEIAHGHPVDQNLMFAGHNAFRFATDPFFTSGRIPTMGELVERILTGD
>Mature_465_residues
MKDIKPLLISGKEVYPLVEGGKGVAVTNGRSSGAWAAAGGVGTISAVNADFYDETGALANQVYKGRTRSERHRELIDFGI
KGGIVQARIAHEEARGEGRMHINVLWEMGGAREVLEGVLEGAKGLVHGVTCGAGMPYGVAEIAARHRVYYYPIVSSARAF
RALWKRAYSKAPEWLGGVVYEDPWLAGGHNGLSNSENPREPQPPLPRVAELRAQMRAVGAPEVPIIMAGGVWYLREWAEW
LENPELGPIAFQFGTRPLLTQESPISDEWKQRLLTLRPGDVLLHRFSPTGFYSSAVRNDFLQELVERSNRQITYFTEPQG
QHTTSFAVGPRAREVFVRAEDSVLAHAWVAQGFTEAMRTPDNSLIFVTPERAERIKTDQINCMGCLSACGFSNWAENELN
NTGKRADPRSFCIQKTLQEIAHGHPVDQNLMFAGHNAFRFATDPFFTSGRIPTMGELVERILTGD

Specific function: Unknown

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.13.11.32

Molecular weight: Translated: 51375; Mature: 51375

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIKPLLISGKEVYPLVEGGKGVAVTNGRSSGAWAAAGGVGTISAVNADFYDETGALAN
CCCCCCCEECCCEEEEEEECCCEEEEECCCCCCCEEECCCCCEEEEECCCHHHHHHHHHH
QVYKGRTRSERHRELIDFGIKGGIVQARIAHEEARGEGRMHINVLWEMGGAREVLEGVLE
HHHCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHH
GAKGLVHGVTCGAGMPYGVAEIAARHRVYYYPIVSSARAFRALWKRAYSKAPEWLGGVVY
HHHHHHHEEECCCCCCCCHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHCCHHHHCCCEE
EDPWLAGGHNGLSNSENPREPQPPLPRVAELRAQMRAVGAPEVPIIMAGGVWYLREWAEW
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHH
LENPELGPIAFQFGTRPLLTQESPISDEWKQRLLTLRPGDVLLHRFSPTGFYSSAVRNDF
HCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHEECCCCCEEEEECCCCCHHHHHHHHHH
LQELVERSNRQITYFTEPQGQHTTSFAVGPRAREVFVRAEDSVLAHAWVAQGFTEAMRTP
HHHHHHHCCCEEEEEECCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCC
DNSLIFVTPERAERIKTDQINCMGCLSACGFSNWAENELNNTGKRADPRSFCIQKTLQEI
CCCEEEEECHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHH
AHGHPVDQNLMFAGHNAFRFATDPFFTSGRIPTMGELVERILTGD
HCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKDIKPLLISGKEVYPLVEGGKGVAVTNGRSSGAWAAAGGVGTISAVNADFYDETGALAN
CCCCCCCEECCCEEEEEEECCCEEEEECCCCCCCEEECCCCCEEEEECCCHHHHHHHHHH
QVYKGRTRSERHRELIDFGIKGGIVQARIAHEEARGEGRMHINVLWEMGGAREVLEGVLE
HHHCCCCHHHHHHHHHHHCCCCCEEEEHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHH
GAKGLVHGVTCGAGMPYGVAEIAARHRVYYYPIVSSARAFRALWKRAYSKAPEWLGGVVY
HHHHHHHEEECCCCCCCCHHHHHHHCEEEEEEHHHHHHHHHHHHHHHHHCCHHHHCCCEE
EDPWLAGGHNGLSNSENPREPQPPLPRVAELRAQMRAVGAPEVPIIMAGGVWYLREWAEW
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHH
LENPELGPIAFQFGTRPLLTQESPISDEWKQRLLTLRPGDVLLHRFSPTGFYSSAVRNDF
HCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHEECCCCCEEEEECCCCCHHHHHHHHHH
LQELVERSNRQITYFTEPQGQHTTSFAVGPRAREVFVRAEDSVLAHAWVAQGFTEAMRTP
HHHHHHHCCCEEEEEECCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHCC
DNSLIFVTPERAERIKTDQINCMGCLSACGFSNWAENELNNTGKRADPRSFCIQKTLQEI
CCCEEEEECHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHH
AHGHPVDQNLMFAGHNAFRFATDPFFTSGRIPTMGELVERILTGD
HCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-nitropropane; O2

Specific reaction: 2 2-nitropropane + O2 = 2 acetone + 2 nitrite

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA