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The map label for this gene is engD [H]

Identifier: 83595077

GI number: 83595077

Start: 4296213

End: 4297316

Strand: Direct

Name: engD [H]

Synonym: Rru_A3748

Alternate gene names: 83595077

Gene position: 4296213-4297316 (Clockwise)

Preceding gene: 83595076

Following gene: 83595078

Centisome position: 98.7

GC content: 64.31

Gene sequence:

>1104_bases
ATGGGATTCAAATGCGGCATCGTTGGTCTGCCCAACGTCGGCAAATCGACTCTTTTCAACGCCCTGACCTCGACCGCCGC
CGCCCAGGCGGCCAATTTTCCCTTTTGCACCATCGAGCCCAATGTCGGGCGGGTGGCGGTGCCCGATCCGCGGCTTGATA
CCCTGGTCGAGATCGGCAAAAGCGCCAATAAGGTGCCGACCCAGCTTGAATTCGTCGATATCGCCGGTTTGGTGCGCGGC
GCCTCGAAGGGCGAGGGGCTGGGCAACCAGTTCCTGGCCAATATCCGCGAGGTCGACGCCATCGTCCATGTGCTGCGCTG
CTTTGAAGACGGCGACATCACTCATGTCGATGGCTCGGTCGATCCCTTGCGCGATGCCGAGACGGTCGAGACCGAGCTGA
TGCTGGCCGACTTGGAAAGCCTGGAAAAGCGCCATTACAATCTGTCCAAGAAGGCCAAGGGCGGCGATATGGACGCCAAG
GCGCTGATTGGCGTGATCGAGCCGGCGCTCGAGGCCTTGCGCGCGGGCAAGACGGCGCGCACCGTCGCCCCGCGCGATGC
CGAGGAGCGCAAGCTGTTCGCCCAGCTTCAGCTTCTCACCTCCAAGCCGGTGCTGTACGTCTGCAATGTCGAGGAGGCCT
CGGCCGCTTCGGGCAACGCGCTGTCGGCCAAGGTCGCCGATCAGGCGGCCAAGGAAGGCGCCCTGGCCGTGGTCATCAGC
GCCGCCATCGAATCCGAGGTCGCCCAGCTTGAAGAAGAAGCCGAGAAGGCCGAGTTCCTGGAAAGCCTCGGTCTCGCGGA
AACCGGCCTGACCCGGGTGATCCGCGCCGGCTATGACCTGCTGGAGCTGATCACCTTCTTTACTTGCGGCCCCAAGGAAG
CCCGCGCCTGGACGGTGCGGCGCGGGTCGCGCGCCCCCCAGGCCGCCGGCGTCATCCATAGCGATTTCGAACGCGGCTTC
ATCCGCGCCGAAACCATCGCCTATGCCGATTACATCGCCCAGGGCGGCGAAAGCGGCGCCAAGGAAGCGGGCAAGATGCG
CTCGGAAGGCAAGGACTACGTGGTCCAGGACGGCGACGTCTTCCTGTTCCGCTTCAACGTCTAA

Upstream 100 bases:

>100_bases
GGTGGCCGAGGTCGGCGCCCCACCCCCCTCTCCTACGGGTCTTGCCGCAGCGCTCGCCGAGGCCCTCGACCGTAAAAGCC
AGAAAGGGAACGGCTGAGCC

Downstream 100 bases:

>100_bases
GGACTATTGGCCGATCTCGGCATCGTTGAGGTCGGCCAAGCATCTGAACACGGCCGTTTTAAGCTAGGCTGAAGGAGGGC
GCGGTGCTGATCAAATCGAT

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MGFKCGIVGLPNVGKSTLFNALTSTAAAQAANFPFCTIEPNVGRVAVPDPRLDTLVEIGKSANKVPTQLEFVDIAGLVRG
ASKGEGLGNQFLANIREVDAIVHVLRCFEDGDITHVDGSVDPLRDAETVETELMLADLESLEKRHYNLSKKAKGGDMDAK
ALIGVIEPALEALRAGKTARTVAPRDAEERKLFAQLQLLTSKPVLYVCNVEEASAASGNALSAKVADQAAKEGALAVVIS
AAIESEVAQLEEEAEKAEFLESLGLAETGLTRVIRAGYDLLELITFFTCGPKEARAWTVRRGSRAPQAAGVIHSDFERGF
IRAETIAYADYIAQGGESGAKEAGKMRSEGKDYVVQDGDVFLFRFNV

Sequences:

>Translated_367_residues
MGFKCGIVGLPNVGKSTLFNALTSTAAAQAANFPFCTIEPNVGRVAVPDPRLDTLVEIGKSANKVPTQLEFVDIAGLVRG
ASKGEGLGNQFLANIREVDAIVHVLRCFEDGDITHVDGSVDPLRDAETVETELMLADLESLEKRHYNLSKKAKGGDMDAK
ALIGVIEPALEALRAGKTARTVAPRDAEERKLFAQLQLLTSKPVLYVCNVEEASAASGNALSAKVADQAAKEGALAVVIS
AAIESEVAQLEEEAEKAEFLESLGLAETGLTRVIRAGYDLLELITFFTCGPKEARAWTVRRGSRAPQAAGVIHSDFERGF
IRAETIAYADYIAQGGESGAKEAGKMRSEGKDYVVQDGDVFLFRFNV
>Mature_366_residues
GFKCGIVGLPNVGKSTLFNALTSTAAAQAANFPFCTIEPNVGRVAVPDPRLDTLVEIGKSANKVPTQLEFVDIAGLVRGA
SKGEGLGNQFLANIREVDAIVHVLRCFEDGDITHVDGSVDPLRDAETVETELMLADLESLEKRHYNLSKKAKGGDMDAKA
LIGVIEPALEALRAGKTARTVAPRDAEERKLFAQLQLLTSKPVLYVCNVEEASAASGNALSAKVADQAAKEGALAVVISA
AIESEVAQLEEEAEKAEFLESLGLAETGLTRVIRAGYDLLELITFFTCGPKEARAWTVRRGSRAPQAAGVIHSDFERGFI
RAETIAYADYIAQGGESGAKEAGKMRSEGKDYVVQDGDVFLFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=369, Percent_Identity=45.2574525745258, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI58761502, Length=193, Percent_Identity=39.8963730569948, Blast_Score=156, Evalue=4e-38,
Organism=Escherichia coli, GI1787454, Length=367, Percent_Identity=59.1280653950954, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI1789574, Length=130, Percent_Identity=33.8461538461538, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17509631, Length=369, Percent_Identity=43.0894308943089, Blast_Score=283, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6319499, Length=374, Percent_Identity=40.9090909090909, Blast_Score=275, Evalue=8e-75,
Organism=Saccharomyces cerevisiae, GI6321773, Length=380, Percent_Identity=38.4210526315789, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6321649, Length=159, Percent_Identity=37.7358490566038, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640873, Length=369, Percent_Identity=44.9864498644986, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24640877, Length=369, Percent_Identity=44.9864498644986, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24640875, Length=369, Percent_Identity=44.9864498644986, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24640879, Length=327, Percent_Identity=42.5076452599388, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 39231; Mature: 39100

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKCGIVGLPNVGKSTLFNALTSTAAAQAANFPFCTIEPNVGRVAVPDPRLDTLVEIGK
CCEEECEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCHHHHHHHCC
SANKVPTQLEFVDIAGLVRGASKGEGLGNQFLANIREVDAIVHVLRCFEDGDITHVDGSV
CCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
DPLRDAETVETELMLADLESLEKRHYNLSKKAKGGDMDAKALIGVIEPALEALRAGKTAR
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
TVAPRDAEERKLFAQLQLLTSKPVLYVCNVEEASAASGNALSAKVADQAAKEGALAVVIS
CCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCHHHHHHHHHHHCCCEEEHHH
AAIESEVAQLEEEAEKAEFLESLGLAETGLTRVIRAGYDLLELITFFTCGPKEARAWTVR
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHCEEEEC
RGSRAPQAAGVIHSDFERGFIRAETIAYADYIAQGGESGAKEAGKMRSEGKDYVVQDGDV
CCCCCCHHHCCHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCE
FLFRFNV
EEEEECC
>Mature Secondary Structure 
GFKCGIVGLPNVGKSTLFNALTSTAAAQAANFPFCTIEPNVGRVAVPDPRLDTLVEIGK
CEEECEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCHHHHHHHCC
SANKVPTQLEFVDIAGLVRGASKGEGLGNQFLANIREVDAIVHVLRCFEDGDITHVDGSV
CCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
DPLRDAETVETELMLADLESLEKRHYNLSKKAKGGDMDAKALIGVIEPALEALRAGKTAR
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
TVAPRDAEERKLFAQLQLLTSKPVLYVCNVEEASAASGNALSAKVADQAAKEGALAVVIS
CCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCHHHHHHHHHHHCCCEEEHHH
AAIESEVAQLEEEAEKAEFLESLGLAETGLTRVIRAGYDLLELITFFTCGPKEARAWTVR
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCHHCEEEEC
RGSRAPQAAGVIHSDFERGFIRAETIAYADYIAQGGESGAKEAGKMRSEGKDYVVQDGDV
CCCCCCHHHCCHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCE
FLFRFNV
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]