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The map label for this gene is gluQ [H]

Identifier: 83595063

GI number: 83595063

Start: 4284486

End: 4285367

Strand: Direct

Name: gluQ [H]

Synonym: Rru_A3734

Alternate gene names: 83595063

Gene position: 4284486-4285367 (Clockwise)

Preceding gene: 83595060

Following gene: 83595064

Centisome position: 98.43

GC content: 70.18

Gene sequence:

>882_bases
ATGACTCGCCGCGACGCGGCGAAAGCCCCCGCCGATGCCCCGACCACCGGCCGCTTCGCCCCCAGTCCGACGGGGCTGCT
GCATCTGGGTCATGCCTATTCGGCCCTGCTCGCCCATGACAGCCGGCGGGCCGAGGGCGGGCGCTTCCTGCTGCGCCTGG
AGGATATCGATCCCCAGCGCTGCCGCCCCGAGTTCGAGACAGCGATCCTTGAGGATCTGACGTGGCTGGGGGTGCGCTGG
GACGCCACGCCGCGCCGCCAGTCCGAACATTGGGCCGATTACCGCGCCGCCCTGGCCCGTTTGGACGAGGCCGGGCTGCT
TTACCGCTGCTTTTGCACAAGGTCGGAAATCGCCGCCGAGGTGGCGCGCAGCCCTTCGGCCCCCCACGGCCCGGAAGGCG
CGCCCTATCCCGGCCTGTGCAAGCGCCTGGACCCCGCCGAGGTCGCCGATCGACTGGCCAAAGGCGACGCCTATGCCCTG
CGTCTGGATACGGCCAAGGCCCTGGCGCGCTGCGGTCCTTTGGTCTGGCACGACGCCGAGGCCGGGGCGGTGGTCGCCCG
TCCCGATCTGTTTGGCGATGTGGTGCTGGCCCGCAAGGATACCCCGACCAGCTACCATCTGGCCGTCACCGTTGATGACG
CCTTGCAGCAAATCACCCATGTGGTGCGCGGCCGCGACCTGTTCGAGGCCACCCATGTCCACCGCCTGCTTCAGGCCCTG
CTCGCCCTGCCCAGCCCGCTTTATCGCCACCACGCCCTGCTCAAAGGCGCGGATGGCAAACGCTACGCCAAGCGCGACCG
GTCCCTGACCCTGGCCAGCCTGCGCGCCACCGGCGAAACCCCGGCCACCCTGCGCCAACGCATCGGCCTGCCTGCGGTTT
AA

Upstream 100 bases:

>100_bases
CCTTCCACCGCAAAGCGAATATGATAGTTCAGTAAAAACGTATAAAGAAAACAATGGCTAGCCGTTCCGCCGGTTCCCCC
GTTTTTCCCCGGTCGACCCG

Downstream 100 bases:

>100_bases
GGGGGGGGCTTGCCGGGCGGCAAGATGCCGCCTATCTATATGGCATGTTGCCGATAGCCTGGGAGGTTCTGATGTCCGAA
GCCCTTATCGAAGCCGTGGC

Product: glutamyl-tRNA synthetase, class Ic

Products: AMP; L-Glutamyl-tRNA(Glu); Diphosphate [C]

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTRRDAAKAPADAPTTGRFAPSPTGLLHLGHAYSALLAHDSRRAEGGRFLLRLEDIDPQRCRPEFETAILEDLTWLGVRW
DATPRRQSEHWADYRAALARLDEAGLLYRCFCTRSEIAAEVARSPSAPHGPEGAPYPGLCKRLDPAEVADRLAKGDAYAL
RLDTAKALARCGPLVWHDAEAGAVVARPDLFGDVVLARKDTPTSYHLAVTVDDALQQITHVVRGRDLFEATHVHRLLQAL
LALPSPLYRHHALLKGADGKRYAKRDRSLTLASLRATGETPATLRQRIGLPAV

Sequences:

>Translated_293_residues
MTRRDAAKAPADAPTTGRFAPSPTGLLHLGHAYSALLAHDSRRAEGGRFLLRLEDIDPQRCRPEFETAILEDLTWLGVRW
DATPRRQSEHWADYRAALARLDEAGLLYRCFCTRSEIAAEVARSPSAPHGPEGAPYPGLCKRLDPAEVADRLAKGDAYAL
RLDTAKALARCGPLVWHDAEAGAVVARPDLFGDVVLARKDTPTSYHLAVTVDDALQQITHVVRGRDLFEATHVHRLLQAL
LALPSPLYRHHALLKGADGKRYAKRDRSLTLASLRATGETPATLRQRIGLPAV
>Mature_292_residues
TRRDAAKAPADAPTTGRFAPSPTGLLHLGHAYSALLAHDSRRAEGGRFLLRLEDIDPQRCRPEFETAILEDLTWLGVRWD
ATPRRQSEHWADYRAALARLDEAGLLYRCFCTRSEIAAEVARSPSAPHGPEGAPYPGLCKRLDPAEVADRLAKGDAYALR
LDTAKALARCGPLVWHDAEAGAVVARPDLFGDVVLARKDTPTSYHLAVTVDDALQQITHVVRGRDLFEATHVHRLLQALL
ALPSPLYRHHALLKGADGKRYAKRDRSLTLASLRATGETPATLRQRIGLPAV

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=280, Percent_Identity=33.9285714285714, Blast_Score=124, Evalue=8e-29,
Organism=Escherichia coli, GI1788743, Length=267, Percent_Identity=32.2097378277154, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI226510925, Length=284, Percent_Identity=33.0985915492958, Blast_Score=121, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71993394, Length=258, Percent_Identity=31.0077519379845, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324540, Length=277, Percent_Identity=32.4909747292419, Blast_Score=145, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24665321, Length=278, Percent_Identity=34.1726618705036, Blast_Score=134, Evalue=6e-32,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17 [C]

Molecular weight: Translated: 32157; Mature: 32026

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRDAAKAPADAPTTGRFAPSPTGLLHLGHAYSALLAHDSRRAEGGRFLLRLEDIDPQR
CCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHH
CRPEFETAILEDLTWLGVRWDATPRRQSEHWADYRAALARLDEAGLLYRCFCTRSEIAAE
CCHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHH
VARSPSAPHGPEGAPYPGLCKRLDPAEVADRLAKGDAYALRLDTAKALARCGPLVWHDAE
HHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCEEECCC
AGAVVARPDLFGDVVLARKDTPTSYHLAVTVDDALQQITHVVRGRDLFEATHVHRLLQAL
CCEEEECCCCHHCEEEEECCCCCEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LALPSPLYRHHALLKGADGKRYAKRDRSLTLASLRATGETPATLRQRIGLPAV
HHCCCHHHHHHHHHCCCCCHHHHHHCCCEEEEEHHCCCCCHHHHHHHCCCCCC
>Mature Secondary Structure 
TRRDAAKAPADAPTTGRFAPSPTGLLHLGHAYSALLAHDSRRAEGGRFLLRLEDIDPQR
CCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHH
CRPEFETAILEDLTWLGVRWDATPRRQSEHWADYRAALARLDEAGLLYRCFCTRSEIAAE
CCHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHH
VARSPSAPHGPEGAPYPGLCKRLDPAEVADRLAKGDAYALRLDTAKALARCGPLVWHDAE
HHHCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCEEEEEEHHHHHHHHCCCCEEECCC
AGAVVARPDLFGDVVLARKDTPTSYHLAVTVDDALQQITHVVRGRDLFEATHVHRLLQAL
CCEEEECCCCHHCEEEEECCCCCEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LALPSPLYRHHALLKGADGKRYAKRDRSLTLASLRATGETPATLRQRIGLPAV
HHCCCHHHHHHHHHCCCCCHHHHHHCCCEEEEEHHCCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-Glutamate; tRNA (Glu) [C]

Specific reaction: ATP + L-Glutamate + tRNA (Glu) --> AMP + L-Glutamyl-tRNA(Glu) + Diphosphate [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA