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The map label for this gene is mnmG

Identifier: 83594954

GI number: 83594954

Start: 4164772

End: 4166661

Strand: Direct

Name: mnmG

Synonym: Rru_A3625

Alternate gene names: 83594954

Gene position: 4164772-4166661 (Clockwise)

Preceding gene: 83594953

Following gene: 83594955

Centisome position: 95.68

GC content: 68.89

Gene sequence:

>1890_bases
ATGACGAACCATTGGGATGTGATCGTGGTTGGCGGCGGCCACGCCGGCACCGAGGCGGCGGCGGCGGCGGCGCGGCTGGG
GGCGAAGACCCTGCTGGCCACCCATAAGCTGGAAACCGTCGGCACCATGTCGTGCAATCCGGCGATCGGCGGTCTGGCCA
AGGGCCATCTGGTGCGCGAGATCGACGCCCTGGATGGGGTGATGGGCCGGGCCATCGATCGCGGCGGCATCCAGTTTCGC
ATCCTCAACCGCTCCAAGGGACCGGCGGTGCGCGGTCCGCGCGCCCAGGCCGATCGGGCGCTTTACGCCCAGGCGGTGCG
GGCGATCCTGGCCGATCAGCCGGGGCTGACCCTGGCCGCCCTGGCGATCGAGGATCTGTTGATCGGGAACGACGGCCGCT
GCGCCGGGGTGATCGACGCCGAGGGCGGGGTGCACCGCGCCGGGGCGGTGGTGCTGACCACCGGCACCTTCCTGCGCGGC
GTTATTCATATCGGCACCCAGACCACCCCAGCCGGGCGCATCGGCGAGGCGCCGGCCCTTGGGCTATCGGCGACCTTGGC
CCGTCTGGGCTTTCCCTTGGGCCGGCTGAAGACCGGGACGCCGCCGCGCCTGGATGGCCGGACCATCGCCTGGGCGACCC
TGGAAAGCCAGCCCGGCGATGAGCCGCCGCCGCCGTTTTCCTTCCTGACCACGGCGATCACCACGCCGCAAATTTCCTGC
GCCATCACCGAAACCACCGCCGAGACCCATCGCGTGATTCGCGAAAATCTCCATCGGGCGCCGCTTTATTCCGGGCAGAT
CCAGGGGGTGGGGCCGCGCTATTGTCCGAGCATCGAGGACAAGGTGGTGCGCTTCGCCGACCGCGACCGCCATCAAATCT
TCCTCGAGCCCGAGGGGCTGGATGATCCGACGGTCTATCCCAATGGCATTTCCACCAGCCTGCCGATCGATGTTCAACTG
GCCTTGCTCGCCACCATTCCCGGTCTGGAAAAGGCCGAGATGATGCGGCCGGGCTATGCCATCGAATACGACTTCGTCGA
TCCGCGCTGCCTGGGCCCGACGCTGGAGACCGACCGTTTGCCCGGTCTGTTCCTCGCCGGCCAGATCAATGGCACCACCG
GCTATGAGGAAGCCGCCGCCCAAGGGCTGATCGCCGGCCTCAACGCCGCCCGCGTCGCCGGGGCGGGGGAGCGGGCCGCG
CCGATCACCTTCGACCGGGCCGAGGGCTATCTGGGGGTTCTGGTCGACGACCTGATTACCCTGGGCACCACCGAGCCTTA
CCGCATGTTCACCTCGCGGGCGGAATACCGCTTGCTGCTGCGCGCCGATAACGCCGATCTGCGGCTGACCGCCAAGGGCA
TCGCTTTGGGCTGCGTCGGCGCCGCGCGCACCGCCGCCTTCGAGGACAAGCGGGCGGCGTTGACGGCGGCGCGGACGATG
ATCGACGGCTTGGCGCTGACCCCGCCGGACCTGGCCCGGCGCGGCATCGCCGTCAATCAGGACGGTCAGCGGCGGACGCC
GCTGGATCTGCTCTGCCATGCCGATATCGACTGGGCGCGGCTGGTGGCCCTGTGGCCCGAGCTCGGGGCGATCCGCCCCG
ATGTCGCCGAGCAACTGGAAATCGGCGCCCGCTATGCCGGCTATCTGGAGCGTATGCATGGCGATGTCGCCGCCTTTCGC
CGCGATGAGGCCCTGGTTCTGCCCGCCGATCTGGCCTATGACGGCCTAGCCAATCTGTCGGCGGAACTGCGCGGCAAGCT
GACTTTGGCCCGGCCGGCGACCCTTGGCGCGGCGGCGCGCATTCCCGGTATGACCCCGGCGGCCTTGACGGCTCTGCTCC
GACATGTGAAGAGAAGGGAGAGAGACTCGGTGGAAGAGTGTTTCACGTGA

Upstream 100 bases:

>100_bases
TTCTTGATGTCATCTTCCGCGATTTCTGCATCGGCAAATAAAGAGCACAGCCCGGCCGCCCCCGCTGAAGGGGGCAGGCC
CGAGAGAGGGACGGGACCCC

Downstream 100 bases:

>100_bases
AACAGTTGGCCGCCGACGTTGCGCTTTTTTCCTGGTTTGGATAAGGAGAACCCGGTGCCGCCGATGACGCCCGAGGCCTT
CGCCGCTGACGTTCCTGTTT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MTNHWDVIVVGGGHAGTEAAAAAARLGAKTLLATHKLETVGTMSCNPAIGGLAKGHLVREIDALDGVMGRAIDRGGIQFR
ILNRSKGPAVRGPRAQADRALYAQAVRAILADQPGLTLAALAIEDLLIGNDGRCAGVIDAEGGVHRAGAVVLTTGTFLRG
VIHIGTQTTPAGRIGEAPALGLSATLARLGFPLGRLKTGTPPRLDGRTIAWATLESQPGDEPPPPFSFLTTAITTPQISC
AITETTAETHRVIRENLHRAPLYSGQIQGVGPRYCPSIEDKVVRFADRDRHQIFLEPEGLDDPTVYPNGISTSLPIDVQL
ALLATIPGLEKAEMMRPGYAIEYDFVDPRCLGPTLETDRLPGLFLAGQINGTTGYEEAAAQGLIAGLNAARVAGAGERAA
PITFDRAEGYLGVLVDDLITLGTTEPYRMFTSRAEYRLLLRADNADLRLTAKGIALGCVGAARTAAFEDKRAALTAARTM
IDGLALTPPDLARRGIAVNQDGQRRTPLDLLCHADIDWARLVALWPELGAIRPDVAEQLEIGARYAGYLERMHGDVAAFR
RDEALVLPADLAYDGLANLSAELRGKLTLARPATLGAAARIPGMTPAALTALLRHVKRRERDSVEECFT

Sequences:

>Translated_629_residues
MTNHWDVIVVGGGHAGTEAAAAAARLGAKTLLATHKLETVGTMSCNPAIGGLAKGHLVREIDALDGVMGRAIDRGGIQFR
ILNRSKGPAVRGPRAQADRALYAQAVRAILADQPGLTLAALAIEDLLIGNDGRCAGVIDAEGGVHRAGAVVLTTGTFLRG
VIHIGTQTTPAGRIGEAPALGLSATLARLGFPLGRLKTGTPPRLDGRTIAWATLESQPGDEPPPPFSFLTTAITTPQISC
AITETTAETHRVIRENLHRAPLYSGQIQGVGPRYCPSIEDKVVRFADRDRHQIFLEPEGLDDPTVYPNGISTSLPIDVQL
ALLATIPGLEKAEMMRPGYAIEYDFVDPRCLGPTLETDRLPGLFLAGQINGTTGYEEAAAQGLIAGLNAARVAGAGERAA
PITFDRAEGYLGVLVDDLITLGTTEPYRMFTSRAEYRLLLRADNADLRLTAKGIALGCVGAARTAAFEDKRAALTAARTM
IDGLALTPPDLARRGIAVNQDGQRRTPLDLLCHADIDWARLVALWPELGAIRPDVAEQLEIGARYAGYLERMHGDVAAFR
RDEALVLPADLAYDGLANLSAELRGKLTLARPATLGAAARIPGMTPAALTALLRHVKRRERDSVEECFT
>Mature_628_residues
TNHWDVIVVGGGHAGTEAAAAAARLGAKTLLATHKLETVGTMSCNPAIGGLAKGHLVREIDALDGVMGRAIDRGGIQFRI
LNRSKGPAVRGPRAQADRALYAQAVRAILADQPGLTLAALAIEDLLIGNDGRCAGVIDAEGGVHRAGAVVLTTGTFLRGV
IHIGTQTTPAGRIGEAPALGLSATLARLGFPLGRLKTGTPPRLDGRTIAWATLESQPGDEPPPPFSFLTTAITTPQISCA
ITETTAETHRVIRENLHRAPLYSGQIQGVGPRYCPSIEDKVVRFADRDRHQIFLEPEGLDDPTVYPNGISTSLPIDVQLA
LLATIPGLEKAEMMRPGYAIEYDFVDPRCLGPTLETDRLPGLFLAGQINGTTGYEEAAAQGLIAGLNAARVAGAGERAAP
ITFDRAEGYLGVLVDDLITLGTTEPYRMFTSRAEYRLLLRADNADLRLTAKGIALGCVGAARTAAFEDKRAALTAARTMI
DGLALTPPDLARRGIAVNQDGQRRTPLDLLCHADIDWARLVALWPELGAIRPDVAEQLEIGARYAGYLERMHGDVAAFRR
DEALVLPADLAYDGLANLSAELRGKLTLARPATLGAAARIPGMTPAALTALLRHVKRRERDSVEECFT

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=641, Percent_Identity=46.6458658346334, Blast_Score=540, Evalue=1e-153,
Organism=Homo sapiens, GI19882217, Length=666, Percent_Identity=44.8948948948949, Blast_Score=525, Evalue=1e-149,
Organism=Homo sapiens, GI183227703, Length=681, Percent_Identity=43.9060205580029, Blast_Score=521, Evalue=1e-147,
Organism=Escherichia coli, GI2367273, Length=624, Percent_Identity=54.9679487179487, Blast_Score=643, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=619, Percent_Identity=43.7802907915994, Blast_Score=488, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6321202, Length=643, Percent_Identity=44.7900466562986, Blast_Score=526, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24658174, Length=634, Percent_Identity=50.4731861198738, Blast_Score=566, Evalue=1e-161,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_RHORT (Q2RN76)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428706.1
- HSSP:   Q5SH33
- ProteinModelPortal:   Q2RN76
- SMR:   Q2RN76
- STRING:   Q2RN76
- GeneID:   3837081
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3625
- NMPDR:   fig|1085.1.peg.1291
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- PhylomeDB:   Q2RN76
- ProtClustDB:   PRK05192
- BioCyc:   RRUB269796:RRU_A3625-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 66792; Mature: 66661

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNHWDVIVVGGGHAGTEAAAAAARLGAKTLLATHKLETVGTMSCNPAIGGLAKGHLVRE
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHCHHHHH
IDALDGVMGRAIDRGGIQFRILNRSKGPAVRGPRAQADRALYAQAVRAILADQPGLTLAA
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHH
LAIEDLLIGNDGRCAGVIDAEGGVHRAGAVVLTTGTFLRGVIHIGTQTTPAGRIGEAPAL
HHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCHHHHHHEEECCCCCCCCCCCCCCCC
GLSATLARLGFPLGRLKTGTPPRLDGRTIAWATLESQPGDEPPPPFSFLTTAITTPQISC
CHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEE
AITETTAETHRVIRENLHRAPLYSGQIQGVGPRYCPSIEDKVVRFADRDRHQIFLEPEGL
EEECCHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCC
DDPTVYPNGISTSLPIDVQLALLATIPGLEKAEMMRPGYAIEYDFVDPRCLGPTLETDRL
CCCCCCCCCCCCCCCCEEEEEEEHHCCCCCHHHHCCCCCEEEECCCCCHHCCCCCCCCCC
PGLFLAGQINGTTGYEEAAAQGLIAGLNAARVAGAGERAAPITFDRAEGYLGVLVDDLIT
CCEEEEEEECCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHH
LGTTEPYRMFTSRAEYRLLLRADNADLRLTAKGIALGCVGAARTAAFEDKRAALTAARTM
HCCCCHHHHHHCCCCEEEEEEECCCCEEEEECCEEEEECHHHHHHHHHHHHHHHHHHHHH
IDGLALTPPDLARRGIAVNQDGQRRTPLDLLCHADIDWARLVALWPELGAIRPDVAEQLE
HCCCCCCCHHHHHCCEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHH
IGARYAGYLERMHGDVAAFRRDEALVLPADLAYDGLANLSAELRGKLTLARPATLGAAAR
HHHHHHHHHHHHHCHHHHHCCCCEEEEEHHHHHHHHHHCCHHHCCEEEEECCCCCCCHHC
IPGMTPAALTALLRHVKRRERDSVEECFT
CCCCCHHHHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
TNHWDVIVVGGGHAGTEAAAAAARLGAKTLLATHKLETVGTMSCNPAIGGLAKGHLVRE
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHCHHHHH
IDALDGVMGRAIDRGGIQFRILNRSKGPAVRGPRAQADRALYAQAVRAILADQPGLTLAA
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHH
LAIEDLLIGNDGRCAGVIDAEGGVHRAGAVVLTTGTFLRGVIHIGTQTTPAGRIGEAPAL
HHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCHHHHHHEEECCCCCCCCCCCCCCCC
GLSATLARLGFPLGRLKTGTPPRLDGRTIAWATLESQPGDEPPPPFSFLTTAITTPQISC
CHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEE
AITETTAETHRVIRENLHRAPLYSGQIQGVGPRYCPSIEDKVVRFADRDRHQIFLEPEGL
EEECCHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCC
DDPTVYPNGISTSLPIDVQLALLATIPGLEKAEMMRPGYAIEYDFVDPRCLGPTLETDRL
CCCCCCCCCCCCCCCCEEEEEEEHHCCCCCHHHHCCCCCEEEECCCCCHHCCCCCCCCCC
PGLFLAGQINGTTGYEEAAAQGLIAGLNAARVAGAGERAAPITFDRAEGYLGVLVDDLIT
CCEEEEEEECCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHH
LGTTEPYRMFTSRAEYRLLLRADNADLRLTAKGIALGCVGAARTAAFEDKRAALTAARTM
HCCCCHHHHHHCCCCEEEEEEECCCCEEEEECCEEEEECHHHHHHHHHHHHHHHHHHHHH
IDGLALTPPDLARRGIAVNQDGQRRTPLDLLCHADIDWARLVALWPELGAIRPDVAEQLE
HCCCCCCCHHHHHCCEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHH
IGARYAGYLERMHGDVAAFRRDEALVLPADLAYDGLANLSAELRGKLTLARPATLGAAAR
HHHHHHHHHHHHHCHHHHHCCCCEEEEEHHHHHHHHHHCCHHHCCEEEEECCCCCCCHHC
IPGMTPAALTALLRHVKRRERDSVEECFT
CCCCCHHHHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA