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The map label for this gene is trmE

Identifier: 83594953

GI number: 83594953

Start: 4163345

End: 4164712

Strand: Direct

Name: trmE

Synonym: Rru_A3624

Alternate gene names: 83594953

Gene position: 4163345-4164712 (Clockwise)

Preceding gene: 83594952

Following gene: 83594954

Centisome position: 95.65

GC content: 71.64

Gene sequence:

>1368_bases
ATGACCGTCGATACCATTTTTGCCCCCGCCACCGCCCGTGGTCGGGCGGGGGTCGCGATCGTTCGCTTGTCCGGACCCCG
GGCCGCAACCGCCCTGACGCTTTTGGCCGGCCGGCTGCCCGAAGCCCGCCGGGCGACCCGCGCGGCGCTGCGCTCCCCCC
CCAGCGGCCCAGGGCCCACCGGCCCGGGGCCGGAGGAGGGGGGCGAGGTTCTTGATGACGCCCTGGTGCTGTGGTTTCCG
GCGCCGGCCAGCTTCACCGGCGAGGATGTGGCCGAGCTTCATATCCATGGCGGGCGGGCGGTTCTTGCCGCGGTGCTGGG
GGCCTTGGGCGATCTGCCCGGCCTGCGTCCGGCCGAGGCCGGCGAGTTTTCGCGGCGGGCTTTCCTCAATGGCCGGCTTG
ATCTGACGGCGGCCGAGGCCCTGGCCGATCTGGTCGATGCCGAGACCCAGGCCCAGCGCCGTCAGGCCCTGCGCCAAGCC
GATGGCGCCCTGGTCCGGCTCTATGAGGGCTGGCGGAAGACCGGCATCGGCCTGCTTGCCCATCTTGAAGCGGTGCTTGA
TTTCCCCGATGAGGACCTGCCGCCCGAAGTGGAAACGGCGGTGCGCGGGGGCATCGGCGCCCTGGCCGACGCCCTGGCCG
GTCATCTCGATGACCGTCACCGGGGCGAGCGCCTGCGCGACGGTCTGCAGGTCGCGGTGGTCGGCGCCCCCAATGTCGGC
AAGTCCAGTCTGGTCAATCGGCTGGCCCGGCGCGAGGCGGCGATCGTCTCCGATATCGCCGGCACCACCCGCGATATCGT
CGAGGTCGCCCTCGATCTGGGTGGCTATCCGCTGGTGGTCGCCGATACCGCCGGTTTGCGCGAGACCAGCGACGGCATCG
AGGCCGAAGGCGTGCGCCGGGCCCGGGCCCGGCTGGCCGCCGCCGATCTGACCCTGGCGGTCAGCGATGGCACGGTGGAA
AACGGGGCCGATGATCCGGCCGCCGATTTGACCGGAGAAGCGGTTTTGCGGGTGGTGACCAAGCGCGATCTACTCGATCC
GCGCGCCGTCGAGCGCTGGCTGGCCCGGGGTGCCCTGCCGGTTTCGACGCTAACCGGCGAGGGGCTGGACGCTTTGGAGG
CGGCCCTGGAAAGCCGGGCCCGGGCTTTTTTCGAGGGCGATGGCACCCCGGCCCTGACCCGCCAGCGCCACCGCTCGGCC
CTGATGGAGGCGCGCGCCGCGCTGCGGCGGGCGGCCGAAGCGCCTCTGGCCGAACTGGTGGCCGAGGACCTGCGCCTCGC
CCTGCGCGCCATCGGCCGCATCACCGGACGGGTGACGGTGGATGACGTTCTTGATGTCATCTTCCGCGATTTCTGCATCG
GCAAATAA

Upstream 100 bases:

>100_bases
TCTGGTCGCAATTCGCCAAGGCCGCCAGCGGCGCCACCGGCAAAAGCGACCCCCGCAAACCTCAGGAATAGGGCGGCCCC
GCGGCCGCGACATTGGTTCT

Downstream 100 bases:

>100_bases
AGAGCACAGCCCGGCCGCCCCCGCTGAAGGGGGCAGGCCCGAGAGAGGGACGGGACCCCATGACGAACCATTGGGATGTG
ATCGTGGTTGGCGGCGGCCA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTVDTIFAPATARGRAGVAIVRLSGPRAATALTLLAGRLPEARRATRAALRSPPSGPGPTGPGPEEGGEVLDDALVLWFP
APASFTGEDVAELHIHGGRAVLAAVLGALGDLPGLRPAEAGEFSRRAFLNGRLDLTAAEALADLVDAETQAQRRQALRQA
DGALVRLYEGWRKTGIGLLAHLEAVLDFPDEDLPPEVETAVRGGIGALADALAGHLDDRHRGERLRDGLQVAVVGAPNVG
KSSLVNRLARREAAIVSDIAGTTRDIVEVALDLGGYPLVVADTAGLRETSDGIEAEGVRRARARLAAADLTLAVSDGTVE
NGADDPAADLTGEAVLRVVTKRDLLDPRAVERWLARGALPVSTLTGEGLDALEAALESRARAFFEGDGTPALTRQRHRSA
LMEARAALRRAAEAPLAELVAEDLRLALRAIGRITGRVTVDDVLDVIFRDFCIGK

Sequences:

>Translated_455_residues
MTVDTIFAPATARGRAGVAIVRLSGPRAATALTLLAGRLPEARRATRAALRSPPSGPGPTGPGPEEGGEVLDDALVLWFP
APASFTGEDVAELHIHGGRAVLAAVLGALGDLPGLRPAEAGEFSRRAFLNGRLDLTAAEALADLVDAETQAQRRQALRQA
DGALVRLYEGWRKTGIGLLAHLEAVLDFPDEDLPPEVETAVRGGIGALADALAGHLDDRHRGERLRDGLQVAVVGAPNVG
KSSLVNRLARREAAIVSDIAGTTRDIVEVALDLGGYPLVVADTAGLRETSDGIEAEGVRRARARLAAADLTLAVSDGTVE
NGADDPAADLTGEAVLRVVTKRDLLDPRAVERWLARGALPVSTLTGEGLDALEAALESRARAFFEGDGTPALTRQRHRSA
LMEARAALRRAAEAPLAELVAEDLRLALRAIGRITGRVTVDDVLDVIFRDFCIGK
>Mature_454_residues
TVDTIFAPATARGRAGVAIVRLSGPRAATALTLLAGRLPEARRATRAALRSPPSGPGPTGPGPEEGGEVLDDALVLWFPA
PASFTGEDVAELHIHGGRAVLAAVLGALGDLPGLRPAEAGEFSRRAFLNGRLDLTAAEALADLVDAETQAQRRQALRQAD
GALVRLYEGWRKTGIGLLAHLEAVLDFPDEDLPPEVETAVRGGIGALADALAGHLDDRHRGERLRDGLQVAVVGAPNVGK
SSLVNRLARREAAIVSDIAGTTRDIVEVALDLGGYPLVVADTAGLRETSDGIEAEGVRRARARLAAADLTLAVSDGTVEN
GADDPAADLTGEAVLRVVTKRDLLDPRAVERWLARGALPVSTLTGEGLDALEAALESRARAFFEGDGTPALTRQRHRSAL
MEARAALRRAAEAPLAELVAEDLRLALRAIGRITGRVTVDDVLDVIFRDFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=472, Percent_Identity=46.8220338983051, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI193082993, Length=457, Percent_Identity=47.2647702407002, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI193082988, Length=507, Percent_Identity=43.9842209072978, Blast_Score=337, Evalue=2e-92,
Organism=Escherichia coli, GI2367268, Length=470, Percent_Identity=39.7872340425532, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI87082120, Length=133, Percent_Identity=32.3308270676692, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17507259, Length=461, Percent_Identity=31.6702819956616, Blast_Score=191, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6323665, Length=491, Percent_Identity=35.234215885947, Blast_Score=279, Evalue=6e-76,
Organism=Drosophila melanogaster, GI45550813, Length=486, Percent_Identity=39.3004115226337, Blast_Score=320, Evalue=9e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_RHORT (Q2RN77)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428705.1
- HSSP:   Q9WYA4
- ProteinModelPortal:   Q2RN77
- SMR:   Q2RN77
- STRING:   Q2RN77
- GeneID:   3837080
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3624
- NMPDR:   fig|1085.1.peg.1292
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   LIDNAMV
- PhylomeDB:   Q2RN77
- ProtClustDB:   PRK05291
- BioCyc:   RRUB269796:RRU_A3624-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 47732; Mature: 47600

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1

Important sites: BINDING 22-22 BINDING 92-92 BINDING 132-132 BINDING 455-455

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDTIFAPATARGRAGVAIVRLSGPRAATALTLLAGRLPEARRATRAALRSPPSGPGPT
CCCCCEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
GPGPEEGGEVLDDALVLWFPAPASFTGEDVAELHIHGGRAVLAAVLGALGDLPGLRPAEA
CCCCCCHHHHHHCCEEEEECCCCCCCCCCHHHEEECCCHHHHHHHHHHHHCCCCCCCCCC
GEFSRRAFLNGRLDLTAAEALADLVDAETQAQRRQALRQADGALVRLYEGWRKTGIGLLA
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
HLEAVLDFPDEDLPPEVETAVRGGIGALADALAGHLDDRHRGERLRDGLQVAVVGAPNVG
HHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCC
KSSLVNRLARREAAIVSDIAGTTRDIVEVALDLGGYPLVVADTAGLRETSDGIEAEGVRR
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHH
ARARLAAADLTLAVSDGTVENGADDPAADLTGEAVLRVVTKRDLLDPRAVERWLARGALP
HHHHHHHHHEEEEEECCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
VSTLTGEGLDALEAALESRARAFFEGDGTPALTRQRHRSALMEARAALRRAAEAPLAELV
CHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AEDLRLALRAIGRITGRVTVDDVLDVIFRDFCIGK
HHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVDTIFAPATARGRAGVAIVRLSGPRAATALTLLAGRLPEARRATRAALRSPPSGPGPT
CCCCEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
GPGPEEGGEVLDDALVLWFPAPASFTGEDVAELHIHGGRAVLAAVLGALGDLPGLRPAEA
CCCCCCHHHHHHCCEEEEECCCCCCCCCCHHHEEECCCHHHHHHHHHHHHCCCCCCCCCC
GEFSRRAFLNGRLDLTAAEALADLVDAETQAQRRQALRQADGALVRLYEGWRKTGIGLLA
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
HLEAVLDFPDEDLPPEVETAVRGGIGALADALAGHLDDRHRGERLRDGLQVAVVGAPNVG
HHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCC
KSSLVNRLARREAAIVSDIAGTTRDIVEVALDLGGYPLVVADTAGLRETSDGIEAEGVRR
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHH
ARARLAAADLTLAVSDGTVENGADDPAADLTGEAVLRVVTKRDLLDPRAVERWLARGALP
HHHHHHHHHEEEEEECCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
VSTLTGEGLDALEAALESRARAFFEGDGTPALTRQRHRSALMEARAALRRAAEAPLAELV
CHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AEDLRLALRAIGRITGRVTVDDVLDVIFRDFCIGK
HHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA