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The map label for this gene is rffG [C]

Identifier: 83594905

GI number: 83594905

Start: 4120599

End: 4121606

Strand: Direct

Name: rffG [C]

Synonym: Rru_A3576

Alternate gene names: 83594905

Gene position: 4120599-4121606 (Clockwise)

Preceding gene: 83594904

Following gene: 83594908

Centisome position: 94.66

GC content: 63.0

Gene sequence:

>1008_bases
ATGACCACTCTCGTTACCGGCACCGCCGGTTTCATCGGCAGCCATGTCGCTCTGCGTCTTTTGCAAGAGGGCGAGCAGGT
GGTGGGGATCGATTGTTACACCCCGTATTACGATGTCGGGCTGAAGGAAGCGCGGGTGGCGCGGCTGAAGGCCTTCCCGG
GGTTTTCCGAGCATCGCCTTGATCTAGCCGACGAGGCCGGGGTGGATGCGGTTTTCCGGGCGGCCCGTCCGCGGCGCGTC
ATTCATCTGGCCGCCCAGGCGGGAGTGCGCTACAGCGTGGAAAATCCCCGGGCCTATCTGGAATCGAACCTGATGGGCAC
CTTCTCGGTGCTGGAAGGCTGCCGCAAAACCGGTGTGGAGCATCTGGTCTTCGCCTCGACCAGTTCGGTCTATGGGGCCA
ATAAGACCCAGCCGTTTTCCGAGCATCAGCCGGCCGACCATCCGCTGACCTTCTATGCCGCGACCAAGCGGGCAACCGAG
ATGATGGCGCATTCCTACGCCAATATTTACCAGTTGCCGTCAACCGCACTGCGCTTTTTCACCGTCTATGGCCCCTGGGG
CCGGCCCGACATGGCCTTGTTCCTGTTCACCGAGGCGATGCTGAAGGGCGAGCCGATCCGCGTGTTCAATCACGGCAAGA
TGGTCCGCGACTTCACCTATATCGATGACATCGTCGATGGCATCTTGCGCGCCAGCGCCAAGATCCCGGTGGCGATGGCG
GGCGGGGCGGCGCAGCCCGATCCGGCGGGCAGCCCGGTCGGCCCCTTCCGCGTCTATAACATCGGCAACAGCCAGCCGGT
CGAATTGATGCGCTATATCGAGGTGCTGGAAGGCTGCCTGGGGGTGACGGCCAAGAAAGAGATGCTGCCCATGCAGCTTG
GCGATGTGCCGGGCACCTGGGCCGATGTCAGCGCCCTGGCCGCCGATACCGGCTATGCCCCCAAGATCGGCGTCGAAGAA
GGGGTGCGCCGCTTCGTTGACTGGTATCGGGGATACTACAAGCCCTAA

Upstream 100 bases:

>100_bases
GGCCCTTTTCGCTGACGGCTTTCCACAGCCCCCCCCTTGGCTTATACATCCCGGGCTTCTTTCCTCGCCCGCTCTCTTCA
GCCGCAACGGACCCCAAGGG

Downstream 100 bases:

>100_bases
CCCCTCAGGCCAGCCGGATCACCCCGGCGCCGCAGGCGATCAGCGCCGCCGCCCCCAGCCGCCAGCGACTGGCCTTTTCG
CCGAGGAAAACCACCGAGAT

Product: UDP-glucuronate 5'-epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTTLVTGTAGFIGSHVALRLLQEGEQVVGIDCYTPYYDVGLKEARVARLKAFPGFSEHRLDLADEAGVDAVFRAARPRRV
IHLAAQAGVRYSVENPRAYLESNLMGTFSVLEGCRKTGVEHLVFASTSSVYGANKTQPFSEHQPADHPLTFYAATKRATE
MMAHSYANIYQLPSTALRFFTVYGPWGRPDMALFLFTEAMLKGEPIRVFNHGKMVRDFTYIDDIVDGILRASAKIPVAMA
GGAAQPDPAGSPVGPFRVYNIGNSQPVELMRYIEVLEGCLGVTAKKEMLPMQLGDVPGTWADVSALAADTGYAPKIGVEE
GVRRFVDWYRGYYKP

Sequences:

>Translated_335_residues
MTTLVTGTAGFIGSHVALRLLQEGEQVVGIDCYTPYYDVGLKEARVARLKAFPGFSEHRLDLADEAGVDAVFRAARPRRV
IHLAAQAGVRYSVENPRAYLESNLMGTFSVLEGCRKTGVEHLVFASTSSVYGANKTQPFSEHQPADHPLTFYAATKRATE
MMAHSYANIYQLPSTALRFFTVYGPWGRPDMALFLFTEAMLKGEPIRVFNHGKMVRDFTYIDDIVDGILRASAKIPVAMA
GGAAQPDPAGSPVGPFRVYNIGNSQPVELMRYIEVLEGCLGVTAKKEMLPMQLGDVPGTWADVSALAADTGYAPKIGVEE
GVRRFVDWYRGYYKP
>Mature_334_residues
TTLVTGTAGFIGSHVALRLLQEGEQVVGIDCYTPYYDVGLKEARVARLKAFPGFSEHRLDLADEAGVDAVFRAARPRRVI
HLAAQAGVRYSVENPRAYLESNLMGTFSVLEGCRKTGVEHLVFASTSSVYGANKTQPFSEHQPADHPLTFYAATKRATEM
MAHSYANIYQLPSTALRFFTVYGPWGRPDMALFLFTEAMLKGEPIRVFNHGKMVRDFTYIDDIVDGILRASAKIPVAMAG
GAAQPDPAGSPVGPFRVYNIGNSQPVELMRYIEVLEGCLGVTAKKEMLPMQLGDVPGTWADVSALAADTGYAPKIGVEEG
VRRFVDWYRGYYKP

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=333, Percent_Identity=25.5255255255255, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI56237023, Length=349, Percent_Identity=26.0744985673352, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI56118217, Length=349, Percent_Identity=26.0744985673352, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI189083684, Length=349, Percent_Identity=26.0744985673352, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI42516563, Length=333, Percent_Identity=24.6246246246246, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI193211614, Length=291, Percent_Identity=24.7422680412371, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI8393516, Length=291, Percent_Identity=24.7422680412371, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=27.4285714285714, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788353, Length=356, Percent_Identity=25.8426966292135, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1786974, Length=324, Percent_Identity=26.2345679012346, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788366, Length=161, Percent_Identity=31.6770186335404, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71982038, Length=352, Percent_Identity=25, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71982035, Length=353, Percent_Identity=25.7790368271955, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17539532, Length=335, Percent_Identity=24.4776119402985, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17568069, Length=342, Percent_Identity=24.8538011695906, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17539424, Length=179, Percent_Identity=30.7262569832402, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=179, Percent_Identity=30.7262569832402, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=179, Percent_Identity=30.7262569832402, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=179, Percent_Identity=30.7262569832402, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=179, Percent_Identity=30.7262569832402, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=347, Percent_Identity=25.3602305475504, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19923002, Length=350, Percent_Identity=26, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356223, Length=334, Percent_Identity=26.9461077844311, Blast_Score=101, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 36787; Mature: 36655

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLVTGTAGFIGSHVALRLLQEGEQVVGIDCYTPYYDVGLKEARVARLKAFPGFSEHRL
CCEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCCHHHHHHHHHCCCCCCHHCC
DLADEAGVDAVFRAARPRRVIHLAAQAGVRYSVENPRAYLESNLMGTFSVLEGCRKTGVE
CCCHHCCHHHHHHHCCCHHEEEEEHHCCCEEECCCCHHHHHHCCCHHHHHHHHHHHCCCC
HLVFASTSSVYGANKTQPFSEHQPADHPLTFYAATKRATEMMAHSYANIYQLPSTALRFF
EEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHEEEE
TVYGPWGRPDMALFLFTEAMLKGEPIRVFNHGKMVRDFTYIDDIVDGILRASAKIPVAMA
EEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
GGAAQPDPAGSPVGPFRVYNIGNSQPVELMRYIEVLEGCLGVTAKKEMLPMQLGDVPGTW
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHCCHHCCCCCCCH
ADVSALAADTGYAPKIGVEEGVRRFVDWYRGYYKP
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTLVTGTAGFIGSHVALRLLQEGEQVVGIDCYTPYYDVGLKEARVARLKAFPGFSEHRL
CEEEECCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCCHHHHHHHHHCCCCCCHHCC
DLADEAGVDAVFRAARPRRVIHLAAQAGVRYSVENPRAYLESNLMGTFSVLEGCRKTGVE
CCCHHCCHHHHHHHCCCHHEEEEEHHCCCEEECCCCHHHHHHCCCHHHHHHHHHHHCCCC
HLVFASTSSVYGANKTQPFSEHQPADHPLTFYAATKRATEMMAHSYANIYQLPSTALRFF
EEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHEEEE
TVYGPWGRPDMALFLFTEAMLKGEPIRVFNHGKMVRDFTYIDDIVDGILRASAKIPVAMA
EEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEE
GGAAQPDPAGSPVGPFRVYNIGNSQPVELMRYIEVLEGCLGVTAKKEMLPMQLGDVPGTW
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHCCHHCCCCCCCH
ADVSALAADTGYAPKIGVEEGVRRFVDWYRGYYKP
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]