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The map label for this gene is cspA [H]

Identifier: 83594791

GI number: 83594791

Start: 3987694

End: 3988326

Strand: Direct

Name: cspA [H]

Synonym: Rru_A3462

Alternate gene names: 83594791

Gene position: 3987694-3988326 (Clockwise)

Preceding gene: 83594790

Following gene: 83594792

Centisome position: 91.61

GC content: 65.09

Gene sequence:

>633_bases
ATGCTGTCTCGTAGCACTGCCACGGTTAAGTGGTTCAACGCGACCAAGGGTTTCGGATTCGTTCGCGTCAGCGACGGTGA
ACCCGATGCGTTTCTTCACATCTCTGTCCTTCAACGGGCCGGCTATTCGGAGTTGCCGGAAGGCGCGACGATCGTCTGCG
ATCTCGCGCCTGGCCAGAAGGGTATGCAGGTCAGCGAAATCTACGAAGTCGAAGGCGGATCGCCCGGTGGCTTCGGCGGT
GGCGGCTATGACCGGGGCGGTTATAGCGGCGGCTTCGACCGGGGCGGGTACAACGGCGGCTTCGATCGCGGGGCCGCCGA
TCGGGGCGGCTATGCCGGCGCCACCGACCGGGGTGGCTATGCCGGGGGCGCCGCCGATCGCGGCGGTTACGCCGGGGGAG
CCGACAGGGGCGGCTTCCAGGGCGAGACCGAGATCGATGGGGTGGTCAAGTTCTTCAGCGCCGACAAGGGCTTTGGCTTC
GTCGTCCCCGACGGCGGCGGCAAGGATGTCTATGTCGGATCGCGCACCTTGCAAGATTGCGGGGTCTCGGTTCTTGAGCA
GGGCCAGCGGGTGCGCATGAGCATCCGCATGGGCAAAAAAGGGCCGATGGCCGGTAGTCTCGAACTGATCTGA

Upstream 100 bases:

>100_bases
AGATTTGACGCCAGCCGCCCGTGGTTCCCGCTTGGCCCTGTGCCGGGAAAGGGGTATCACCGGTGGTATAGGTGGGTCCT
CTTGGGGTTAGTTGCGGATC

Downstream 100 bases:

>100_bases
CGCTTTCCCCTGGTTTTTGACCCTATGGGTGGTGCCGGCGCGCGCCACCCATAGGGTTGTCCAATTTTGATCACGGCTGT
CTTGCGATCTGACGGTCTTG

Product: cold-shock DNA-binding protein family protein

Products: NA

Alternate protein names: CSP-A; 7.4 kDa cold shock protein; CS7.4 [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MLSRSTATVKWFNATKGFGFVRVSDGEPDAFLHISVLQRAGYSELPEGATIVCDLAPGQKGMQVSEIYEVEGGSPGGFGG
GGYDRGGYSGGFDRGGYNGGFDRGAADRGGYAGATDRGGYAGGAADRGGYAGGADRGGFQGETEIDGVVKFFSADKGFGF
VVPDGGGKDVYVGSRTLQDCGVSVLEQGQRVRMSIRMGKKGPMAGSLELI

Sequences:

>Translated_210_residues
MLSRSTATVKWFNATKGFGFVRVSDGEPDAFLHISVLQRAGYSELPEGATIVCDLAPGQKGMQVSEIYEVEGGSPGGFGG
GGYDRGGYSGGFDRGGYNGGFDRGAADRGGYAGATDRGGYAGGAADRGGYAGGADRGGFQGETEIDGVVKFFSADKGFGF
VVPDGGGKDVYVGSRTLQDCGVSVLEQGQRVRMSIRMGKKGPMAGSLELI
>Mature_210_residues
MLSRSTATVKWFNATKGFGFVRVSDGEPDAFLHISVLQRAGYSELPEGATIVCDLAPGQKGMQVSEIYEVEGGSPGGFGG
GGYDRGGYSGGFDRGGYNGGFDRGAADRGGYAGATDRGGYAGGAADRGGYAGGADRGGFQGETEIDGVVKFFSADKGFGF
VVPDGGGKDVYVGSRTLQDCGVSVLEQGQRVRMSIRMGKKGPMAGSLELI

Specific function: Binds to and stimulates the transcription of the CCAAT- containing, cold-shock-inducible promoters of the H-NS and GyrA proteins. Binds also to the inverted repeat 5'-ATTGG-3' [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=48.4375, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=43.75, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787839, Length=67, Percent_Identity=43.2835820895522, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 21560; Mature: 21560

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRSTATVKWFNATKGFGFVRVSDGEPDAFLHISVLQRAGYSELPEGATIVCDLAPGQK
CCCCCCEEEEEEECCCCEEEEEECCCCCCEEEEEEHHHHCCCCCCCCCCEEEEECCCCCC
GMQVSEIYEVEGGSPGGFGGGGYDRGGYSGGFDRGGYNGGFDRGAADRGGYAGATDRGGY
CCEEEHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGGAADRGGYAGGADRGGFQGETEIDGVVKFFSADKGFGFVVPDGGGKDVYVGSRTLQDC
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEECCCCCEEEEECCCCCEEEECCCCHHHC
GVSVLEQGQRVRMSIRMGKKGPMAGSLELI
CHHHHHCCCEEEEEEEECCCCCCCCCEEEC
>Mature Secondary Structure
MLSRSTATVKWFNATKGFGFVRVSDGEPDAFLHISVLQRAGYSELPEGATIVCDLAPGQK
CCCCCCEEEEEEECCCCEEEEEECCCCCCEEEEEEHHHHCCCCCCCCCCEEEEECCCCCC
GMQVSEIYEVEGGSPGGFGGGGYDRGGYSGGFDRGGYNGGFDRGAADRGGYAGATDRGGY
CCEEEHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGGAADRGGYAGGADRGGFQGETEIDGVVKFFSADKGFGFVVPDGGGKDVYVGSRTLQDC
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEEECCCCCEEEEECCCCCEEEECCCCHHHC
GVSVLEQGQRVRMSIRMGKKGPMAGSLELI
CHHHHHCCCEEEEEEEECCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]