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The map label for this gene is glpK [H]

Identifier: 83594790

GI number: 83594790

Start: 3985923

End: 3987440

Strand: Direct

Name: glpK [H]

Synonym: Rru_A3461

Alternate gene names: 83594790

Gene position: 3985923-3987440 (Clockwise)

Preceding gene: 83594789

Following gene: 83594791

Centisome position: 91.57

GC content: 67.59

Gene sequence:

>1518_bases
ATGAACGCGAAAGCGCGCAAGAAAATGATTATCCGCGATGACCTGATCCTGGCCATCGACCAGGGCACATCGGGAACGCG
CGCCTTGTTGTTCGACCAGCACGGGCCGGTCCATAGCGGCGGCCGGCTGGCGCTGGCGCCCACCCATCCGCGCGACGGTT
GGGTCGAGATCGATCCCGAGGATATCTGGCTCGACGTGCTGACCGCTTGGCGTCAGGTCCTCGAAGGCAGCGGCGCCGAT
CACGTCACCGCCATCGCCCTGGCCACCCAGCGCGACACCACCATCGTCTGGGACCGGCGTACCGGCAAGCCGGTCTATAA
CGCCATTTCCTGGCTTGATCGGCGGGGCGCGGCGCGCTGCCGGGAGTTGGTGGAGACCGGCTATGAAAGCTTGGTGCGGG
TGCGCAGCGGTCTGGTCGTCGACAGCGCCTTTGCCGCCACCAAGGTGGCCTGGATCCTTGAGAACGTCGAGGGCGCCCGG
GCGGCGGCCGAAGCCGGCCATCTCGCCTTCGGCACCGTCGACAGCTTTCTGCTGTGGCGATTGACCGGCGGCGCCGTTCA
CGCCACCGACGCGTCGAACGCCAGCCGGACGATGCTGTTCAACATCCATAGCCAGCGCTGGGACGCCGATCTGCTGGCGT
TGTTTGGCGTTCCCTGGCAGATGATGCCCGAGGTGCGCGACAGCGCCTGCGTTTTTGGCCATACGGACCGCGCCGGCTTC
GGCCGGCCGATTCCGATCGCCGCGATGATCGGCGATCAAAGCGCCGCCCTGATCGGCCAGGGCTGCCTGACGCCGGGCAT
GATCAAGGCCGGAATGGGGGCGAGTTGCGTGATGATCGCCAATACCGGACCGACGGCGCTGGCCTCCCGCCACGGCCTGC
TGACCTCGATCGCCTATCGGCTCGACGGCGAGGTCACCTATGCGCTGGAAGGGACGGTCTTCAGCGCCGGGGCGGCGATC
GGCTGGCTGAGCGAAAGCCTGGGGGTGATCGAACGCCCCGAGATCACCGAGCTTCTGGCCCGCGGGATGAAAGGCAATCG
CGGGGTGTACATGGTTCCGGCCTTCAGCGGCCTGGGCGCCCCCTATTGGGATCCGGGGGCTCGGGGGGCCATTTTCGGCC
TGTCCCATGACACGGGGGTTCCCGAACTGGCGCGCGCCGCCCTGGAAAGCGTCGCCTATCAGGCCTACGACCTGCTCGAG
GCGATGATCGCCGATGGCCTGCCCAGGCCAGCGGTGCTGCGGGTCGAAGGGGCGATGAGCGCCAATGACTGGCTGCTGTC
GTTTCTGGCCAATATCCTTGAGGTGCCGGTTGAACGACCGGCCATCGCCGAGACGGCGGCCATGGGCGCCGCCCTGCTGG
CGGGGGTCCACCTTGGGCTGTATGGCTCGCTCGACGCGGCCGGCAACGCCTGGGAGGGCAATGGGCGTTTTGACCCGGCG
ATCGATCCGGCCTATCGCCGTCAACTGCTTGACGGCTGGCGCAAAGCCGTCGGACGGGTTCAGGAGTCGGCGCCATGA

Upstream 100 bases:

>100_bases
ATCCGCTGTGGGTCGAGCGTCCGGCGACGGTCGGCGAGGAAGACGACTGGCCCGATAACGAAACCGAAGGCAAGGGACGG
GGCACGGCCTCGCGAAGCGG

Downstream 100 bases:

>100_bases
GGGCCAAGGTGGCGTGGGAGATCTCTACCTAGAAGATGATTGCAAAAAAATTTTCAACCATCCAGAGGTTGGCTACCCCG
GCGCGCAGTAGGGTTTTTCA

Product: carbohydrate kinase, FGGY

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MNAKARKKMIIRDDLILAIDQGTSGTRALLFDQHGPVHSGGRLALAPTHPRDGWVEIDPEDIWLDVLTAWRQVLEGSGAD
HVTAIALATQRDTTIVWDRRTGKPVYNAISWLDRRGAARCRELVETGYESLVRVRSGLVVDSAFAATKVAWILENVEGAR
AAAEAGHLAFGTVDSFLLWRLTGGAVHATDASNASRTMLFNIHSQRWDADLLALFGVPWQMMPEVRDSACVFGHTDRAGF
GRPIPIAAMIGDQSAALIGQGCLTPGMIKAGMGASCVMIANTGPTALASRHGLLTSIAYRLDGEVTYALEGTVFSAGAAI
GWLSESLGVIERPEITELLARGMKGNRGVYMVPAFSGLGAPYWDPGARGAIFGLSHDTGVPELARAALESVAYQAYDLLE
AMIADGLPRPAVLRVEGAMSANDWLLSFLANILEVPVERPAIAETAAMGAALLAGVHLGLYGSLDAAGNAWEGNGRFDPA
IDPAYRRQLLDGWRKAVGRVQESAP

Sequences:

>Translated_505_residues
MNAKARKKMIIRDDLILAIDQGTSGTRALLFDQHGPVHSGGRLALAPTHPRDGWVEIDPEDIWLDVLTAWRQVLEGSGAD
HVTAIALATQRDTTIVWDRRTGKPVYNAISWLDRRGAARCRELVETGYESLVRVRSGLVVDSAFAATKVAWILENVEGAR
AAAEAGHLAFGTVDSFLLWRLTGGAVHATDASNASRTMLFNIHSQRWDADLLALFGVPWQMMPEVRDSACVFGHTDRAGF
GRPIPIAAMIGDQSAALIGQGCLTPGMIKAGMGASCVMIANTGPTALASRHGLLTSIAYRLDGEVTYALEGTVFSAGAAI
GWLSESLGVIERPEITELLARGMKGNRGVYMVPAFSGLGAPYWDPGARGAIFGLSHDTGVPELARAALESVAYQAYDLLE
AMIADGLPRPAVLRVEGAMSANDWLLSFLANILEVPVERPAIAETAAMGAALLAGVHLGLYGSLDAAGNAWEGNGRFDPA
IDPAYRRQLLDGWRKAVGRVQESAP
>Mature_505_residues
MNAKARKKMIIRDDLILAIDQGTSGTRALLFDQHGPVHSGGRLALAPTHPRDGWVEIDPEDIWLDVLTAWRQVLEGSGAD
HVTAIALATQRDTTIVWDRRTGKPVYNAISWLDRRGAARCRELVETGYESLVRVRSGLVVDSAFAATKVAWILENVEGAR
AAAEAGHLAFGTVDSFLLWRLTGGAVHATDASNASRTMLFNIHSQRWDADLLALFGVPWQMMPEVRDSACVFGHTDRAGF
GRPIPIAAMIGDQSAALIGQGCLTPGMIKAGMGASCVMIANTGPTALASRHGLLTSIAYRLDGEVTYALEGTVFSAGAAI
GWLSESLGVIERPEITELLARGMKGNRGVYMVPAFSGLGAPYWDPGARGAIFGLSHDTGVPELARAALESVAYQAYDLLE
AMIADGLPRPAVLRVEGAMSANDWLLSFLANILEVPVERPAIAETAAMGAALLAGVHLGLYGSLDAAGNAWEGNGRFDPA
IDPAYRRQLLDGWRKAVGRVQESAP

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI42794763, Length=515, Percent_Identity=41.747572815534, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI4504007, Length=510, Percent_Identity=41.9607843137255, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI189409120, Length=510, Percent_Identity=41.7647058823529, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI41393575, Length=500, Percent_Identity=40.4, Blast_Score=348, Evalue=7e-96,
Organism=Homo sapiens, GI88196792, Length=503, Percent_Identity=31.013916500994, Blast_Score=268, Evalue=8e-72,
Organism=Escherichia coli, GI1790361, Length=487, Percent_Identity=46.8172484599589, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1789987, Length=428, Percent_Identity=25.9345794392523, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790005, Length=460, Percent_Identity=24.7826086956522, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789138, Length=467, Percent_Identity=22.6980728051392, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535599, Length=498, Percent_Identity=35.9437751004016, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI71986140, Length=517, Percent_Identity=23.9845261121857, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI71986147, Length=298, Percent_Identity=26.8456375838926, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321755, Length=542, Percent_Identity=31.549815498155, Blast_Score=236, Evalue=9e-63,
Organism=Drosophila melanogaster, GI17864214, Length=501, Percent_Identity=36.7265469061876, Blast_Score=318, Evalue=7e-87,
Organism=Drosophila melanogaster, GI24654573, Length=434, Percent_Identity=38.4792626728111, Blast_Score=304, Evalue=9e-83,
Organism=Drosophila melanogaster, GI24655555, Length=508, Percent_Identity=33.2677165354331, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24655560, Length=508, Percent_Identity=33.2677165354331, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24655570, Length=508, Percent_Identity=33.2677165354331, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24655574, Length=508, Percent_Identity=33.2677165354331, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24655565, Length=508, Percent_Identity=33.2677165354331, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI19922058, Length=524, Percent_Identity=31.2977099236641, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24652871, Length=490, Percent_Identity=32.0408163265306, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24656367, Length=515, Percent_Identity=26.7961165048544, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI21357867, Length=530, Percent_Identity=26.6037735849057, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24656372, Length=350, Percent_Identity=28, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI45552909, Length=291, Percent_Identity=26.8041237113402, Blast_Score=109, Evalue=5e-24,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 53896; Mature: 53896

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKARKKMIIRDDLILAIDQGTSGTRALLFDQHGPVHSGGRLALAPTHPRDGWVEIDPE
CCCCHHHCEEEECCEEEEEECCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCEEEECHH
DIWLDVLTAWRQVLEGSGADHVTAIALATQRDTTIVWDRRTGKPVYNAISWLDRRGAARC
HHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
RELVETGYESLVRVRSGLVVDSAFAATKVAWILENVEGARAAAEAGHLAFGTVDSFLLWR
HHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHCCCHHHHHHHCCCEEEHHHHHHHHHE
LTGGAVHATDASNASRTMLFNIHSQRWDADLLALFGVPWQMMPEVRDSACVFGHTDRAGF
ECCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHHCCCHHHHHHHCCCEEEEECCCCCCC
GRPIPIAAMIGDQSAALIGQGCLTPGMIKAGMGASCVMIANTGPTALASRHGLLTSIAYR
CCCCEEEEEECCCCHHHHCCCCCCCCHHHCCCCCEEEEEECCCCHHHHHHCCHHHHHHHH
LDGEVTYALEGTVFSAGAAIGWLSESLGVIERPEITELLARGMKGNRGVYMVPAFSGLGA
CCCEEEEEEECCEEECCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCC
PYWDPGARGAIFGLSHDTGVPELARAALESVAYQAYDLLEAMIADGLPRPAVLRVEGAMS
CCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
ANDWLLSFLANILEVPVERPAIAETAAMGAALLAGVHLGLYGSLDAAGNAWEGNGRFDPA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCC
IDPAYRRQLLDGWRKAVGRVQESAP
CCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNAKARKKMIIRDDLILAIDQGTSGTRALLFDQHGPVHSGGRLALAPTHPRDGWVEIDPE
CCCCHHHCEEEECCEEEEEECCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCEEEECHH
DIWLDVLTAWRQVLEGSGADHVTAIALATQRDTTIVWDRRTGKPVYNAISWLDRRGAARC
HHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
RELVETGYESLVRVRSGLVVDSAFAATKVAWILENVEGARAAAEAGHLAFGTVDSFLLWR
HHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHCCCHHHHHHHCCCEEEHHHHHHHHHE
LTGGAVHATDASNASRTMLFNIHSQRWDADLLALFGVPWQMMPEVRDSACVFGHTDRAGF
ECCCEEEECCCCCCCEEEEEEECCCCCCHHHHHHHCCCHHHHHHHCCCEEEEECCCCCCC
GRPIPIAAMIGDQSAALIGQGCLTPGMIKAGMGASCVMIANTGPTALASRHGLLTSIAYR
CCCCEEEEEECCCCHHHHCCCCCCCCHHHCCCCCEEEEEECCCCHHHHHHCCHHHHHHHH
LDGEVTYALEGTVFSAGAAIGWLSESLGVIERPEITELLARGMKGNRGVYMVPAFSGLGA
CCCEEEEEEECCEEECCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCC
PYWDPGARGAIFGLSHDTGVPELARAALESVAYQAYDLLEAMIADGLPRPAVLRVEGAMS
CCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
ANDWLLSFLANILEVPVERPAIAETAAMGAALLAGVHLGLYGSLDAAGNAWEGNGRFDPA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCC
IDPAYRRQLLDGWRKAVGRVQESAP
CCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA