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The map label for this gene is pyrF

Identifier: 83594753

GI number: 83594753

Start: 3941818

End: 3942561

Strand: Direct

Name: pyrF

Synonym: Rru_A3424

Alternate gene names: 83594753

Gene position: 3941818-3942561 (Clockwise)

Preceding gene: 83594752

Following gene: 83594754

Centisome position: 90.56

GC content: 68.68

Gene sequence:

>744_bases
ATGCCCATGGCACAGATGACGCAAAATCCGGTTTTCGTCGCGATCGACACCACCGTTGTCGGCGATGCGACCACCCTTGC
CGCCCGCCTCCATGACGAGGTCGGCGGCATCAAGCTTGGACTCGAGTTTTTCGCCCGCAATGGGCACAAGGGCGTCAAGG
AGGTGTGCAGGGCCGGCGCCCTGCCGCTGTTCCTTGATCTCAAGTTCCATGACATCCCCAATACGGTGGCCGGGGCGGTG
CGCGCGGTGATGCCGCTGGCGCCGGCCTTGCTCAATGTCCACGCCTGTGGCGGGCGGGCGATGATGATCGCCGCGCGCGA
GGCCGCGCAAAGCGAGGCCCTGGCCCTGGGGATCGCGCCGCCCAAGATGATCGCCGTGACCGTGCTGACCAGCATGGACG
ACGACGATCTGGGCGGAACCGGCGTCGCCGGCGGCGTGCTTGATCAGGTGCGGCGTTTGGCCGCCCTGACCCGCGACGCC
GGGCTTGATGGCGTGGTCTGCTCGGCCCGCGAGGCCCGGGTGCTGCGCGCCGATTTGGGCGATGATTTCTTGCTGGTGAC
GCCGGGGGTGCGGCCGCTGTGGTCGACCACCAACGACCAGAAGCGGGTGGTCACCCCGCAAGAGGCGATGGCCGAGGGGG
CCGATGTGCTGGTGATCGGCCGGCCGATCACCGGGGCGACCGATCCCGCCCAGGCGGCGCGGCTGATCGCCGGGGAAATC
GTGGGATGGGACGTCGGGATATGA

Upstream 100 bases:

>100_bases
AGCCGCGGGCCCCCGGTCGCCCTTGCCCGTCCGCCGAGAAAGAAAGCTTCAAGACAAGTCTCTCCCCGGCCTTTTCGGAC
GTGGGGTTTTCGTGTAAGGC

Downstream 100 bases:

>100_bases
GCGAGACCCTGATCAAGATCTGTGGATTGACCGACGAGGACGGCGTCGATGCCGCCGTCGAAGCCGGGGTCGACATGATC
GGCTTCGTTTTCTTCGAGCC

Product: orotidine-5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MPMAQMTQNPVFVAIDTTVVGDATTLAARLHDEVGGIKLGLEFFARNGHKGVKEVCRAGALPLFLDLKFHDIPNTVAGAV
RAVMPLAPALLNVHACGGRAMMIAAREAAQSEALALGIAPPKMIAVTVLTSMDDDDLGGTGVAGGVLDQVRRLAALTRDA
GLDGVVCSAREARVLRADLGDDFLLVTPGVRPLWSTTNDQKRVVTPQEAMAEGADVLVIGRPITGATDPAQAARLIAGEI
VGWDVGI

Sequences:

>Translated_247_residues
MPMAQMTQNPVFVAIDTTVVGDATTLAARLHDEVGGIKLGLEFFARNGHKGVKEVCRAGALPLFLDLKFHDIPNTVAGAV
RAVMPLAPALLNVHACGGRAMMIAAREAAQSEALALGIAPPKMIAVTVLTSMDDDDLGGTGVAGGVLDQVRRLAALTRDA
GLDGVVCSAREARVLRADLGDDFLLVTPGVRPLWSTTNDQKRVVTPQEAMAEGADVLVIGRPITGATDPAQAARLIAGEI
VGWDVGI
>Mature_246_residues
PMAQMTQNPVFVAIDTTVVGDATTLAARLHDEVGGIKLGLEFFARNGHKGVKEVCRAGALPLFLDLKFHDIPNTVAGAVR
AVMPLAPALLNVHACGGRAMMIAAREAAQSEALALGIAPPKMIAVTVLTSMDDDDLGGTGVAGGVLDQVRRLAALTRDAG
LDGVVCSAREARVLRADLGDDFLLVTPGVRPLWSTTNDQKRVVTPQEAMAEGADVLVIGRPITGATDPAQAARLIAGEIV
GWDVGI

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=235, Percent_Identity=45.531914893617, Blast_Score=179, Evalue=1e-46,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_RHORT (Q2RNS7)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428505.1
- ProteinModelPortal:   Q2RNS7
- STRING:   Q2RNS7
- GeneID:   3836876
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3424
- NMPDR:   fig|1085.1.peg.2073
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   Q2RNS7
- BioCyc:   RRUB269796:RRU_A3424-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25673; Mature: 25542

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 68-68 BINDING 16-16 BINDING 38-38 BINDING 130-130 BINDING 191-191 BINDING 200-200 BINDING 220-220 BINDING 221-221

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMAQMTQNPVFVAIDTTVVGDATTLAARLHDEVGGIKLGLEFFARNGHKGVKEVCRAGA
CCCCHHCCCCEEEEEECEECCCHHHHHHHHHHHCCCEEEEHHHHHCCCCHHHHHHHHCCC
LPLFLDLKFHDIPNTVAGAVRAVMPLAPALLNVHACGGRAMMIAAREAAQSEALALGIAP
CEEEEEEEECCCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEEHHHHCCCEEEEECCC
PKMIAVTVLTSMDDDDLGGTGVAGGVLDQVRRLAALTRDAGLDGVVCSAREARVLRADLG
CHHEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHEEEEECCC
DDFLLVTPGVRPLWSTTNDQKRVVTPQEAMAEGADVLVIGRPITGATDPAQAARLIAGEI
CCEEEECCCCCCCCCCCCCCEEEECCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHH
VGWDVGI
EEEECCC
>Mature Secondary Structure 
PMAQMTQNPVFVAIDTTVVGDATTLAARLHDEVGGIKLGLEFFARNGHKGVKEVCRAGA
CCCHHCCCCEEEEEECEECCCHHHHHHHHHHHCCCEEEEHHHHHCCCCHHHHHHHHCCC
LPLFLDLKFHDIPNTVAGAVRAVMPLAPALLNVHACGGRAMMIAAREAAQSEALALGIAP
CEEEEEEEECCCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEEEHHHHCCCEEEEECCC
PKMIAVTVLTSMDDDDLGGTGVAGGVLDQVRRLAALTRDAGLDGVVCSAREARVLRADLG
CHHEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHEEEEECCC
DDFLLVTPGVRPLWSTTNDQKRVVTPQEAMAEGADVLVIGRPITGATDPAQAARLIAGEI
CCEEEECCCCCCCCCCCCCCEEEECCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHH
VGWDVGI
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA