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The map label for this gene is aphA [H]

Identifier: 83594692

GI number: 83594692

Start: 3875419

End: 3876966

Strand: Reverse

Name: aphA [H]

Synonym: Rru_A3363

Alternate gene names: 83594692

Gene position: 3876966-3875419 (Counterclockwise)

Preceding gene: 83594693

Following gene: 83594691

Centisome position: 89.07

GC content: 67.38

Gene sequence:

>1548_bases
ATGAGCGGCGAGCCCGTCGGTCTGGCCCTGCAACGTCTCTACGACTTGACCCGTCTGGTTTCCGACTGGATCTGGGAGAC
CGATTCCGCCGGCCTTCTGACCTACGCTTCCGACCGGGTTTTTGAAATCCTCGGATATCCGGCCCGGGCCCTGATCGGCC
ACCCCTTCGCCGAACTTGCGGCCTCGGGCGACCCGCCGTTCTTCAAAAGCGATCCGCGGCCCTTCCGCGAGCAGCGCTTC
GAGGCCCGCCACAGCGACGGCTCGCCGCGCGTTCTGCTGATCAGCGCCGTTCCGCTGTTCGATGCGCAAAGCGGCGACCG
CCAGGGCTGGCGCGGCGCGGCCAAGGATATCACCCAAAGCGAGCGGGCCGACGCCGAGATGGACCGCCAGCGCCGTTTCC
AGCAGACCCTGCTTGACGCCATCCCCCTGCCGGTTTTCCTGAAAGACCGCGAGGACCGCTATCAGGGCTGCAATCTGGCT
TTCGAAACCGCCTCGGGCCGCAGCCGCGCCGAAATCCTCGGTCAGCGGATGGAGCAGGTTCTGCGCAGCGAGAATTACGA
CCGCCACCGCGCCGAAGAGGCCCTTGTGCTGCGCGAGGGGCGGACGCGGGTGTGGGAGGCGATGGAACACGACGTCTCGG
GGCGCCACAAGCTGATCTCGATCACCGCCTCGCCCTATCGCGAACCGGGCGATCCCACGGTGCGCGGCTTGATCGGCGTC
GTCTCCGATATCACCCGGCGCCAGCGCGCCGAGGACGCTTTGCGGGCCTCGGTGGTCGAATTGACCGCCTCGAACCGCGA
GCTGCAGCATTTCGCCGAGATCGCCGCCCATGACCTTCAAGAACCGGTGCGCGCCGTGGTCAGCCATTGTCAGCTTCTTG
AACGCCATCTGGGCGACGCCCTGGACGGGCCGGCCCGCGACCATATGATCTTCGCCATCACCGGCGCCCGGCGGATGCGC
GATCTTATCCAGGATCTGTTGATCTATGCCCGGGCCGGTCGCGATGGCCAGTTCCACGAGGTCGTCGACACCGCCGATCT
GGCGCGCGAGGTCGTTTCCGCCCTGGCCGACGAGATCGCCGCCAGCGGCGCGACCATCCGCATCGACCCGCTTCCCAGCG
TTCTCGGGCGGCGGCGCGATTTGTTCGACGTGATGGTCAACCTGTTTGACAACGCCTTGAAGTTCCGCCGCCCCGATGTC
GTTCCCGAGGTCACCCTGAAGGCCGCCGAAACCCTGGATGCCTCGGGTCTGTGGTGCTTCACCCTGAGCGATAATGGCAT
CGGCCTCGACCCCGTCCACGGTGCCCAGATCTTCGCCCTGTTCCGCCGGCTCCATGGCGCCGACCGCTATCCGGGCACCG
GGGTTGGGCTGGCGATCTGTCGGCGAATCGTTGAACAGCACGGCGGCACCATCTTCGTCGAAAGCGCCCCCGGGGTCGGC
ACGCGCTTCCATTTCACCCTTCCCGCCGCCCCCTCCGAGGAGCCGGATCGGCTGGTCGGCCGAGGAACCCGCTCCGCGCC
CCGACCTGTTATCGACGGGGGCGGGTGA

Upstream 100 bases:

>100_bases
CCTGCTGTCGCTTGAAAGCGAGGCCAGCGCCCCCACCCTGGTCTTCGGCCGCCCGGCCTCTTCCCGTCCTTTTCGGCGGC
AACCCAAGGAAAGCTAGGCG

Downstream 100 bases:

>100_bases
AAAAGCCGTCACCCGCCACTGGAAATTCACCATCGCGTCGGTGTAGCGTGCCGCCATGAAACAGTCTTTCGCCTTTTCCC
GACTCGGGTTGGCCCTTCCT

Product: PAS/PAC sensor Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSGEPVGLALQRLYDLTRLVSDWIWETDSAGLLTYASDRVFEILGYPARALIGHPFAELAASGDPPFFKSDPRPFREQRF
EARHSDGSPRVLLISAVPLFDAQSGDRQGWRGAAKDITQSERADAEMDRQRRFQQTLLDAIPLPVFLKDREDRYQGCNLA
FETASGRSRAEILGQRMEQVLRSENYDRHRAEEALVLREGRTRVWEAMEHDVSGRHKLISITASPYREPGDPTVRGLIGV
VSDITRRQRAEDALRASVVELTASNRELQHFAEIAAHDLQEPVRAVVSHCQLLERHLGDALDGPARDHMIFAITGARRMR
DLIQDLLIYARAGRDGQFHEVVDTADLAREVVSALADEIAASGATIRIDPLPSVLGRRRDLFDVMVNLFDNALKFRRPDV
VPEVTLKAAETLDASGLWCFTLSDNGIGLDPVHGAQIFALFRRLHGADRYPGTGVGLAICRRIVEQHGGTIFVESAPGVG
TRFHFTLPAAPSEEPDRLVGRGTRSAPRPVIDGGG

Sequences:

>Translated_515_residues
MSGEPVGLALQRLYDLTRLVSDWIWETDSAGLLTYASDRVFEILGYPARALIGHPFAELAASGDPPFFKSDPRPFREQRF
EARHSDGSPRVLLISAVPLFDAQSGDRQGWRGAAKDITQSERADAEMDRQRRFQQTLLDAIPLPVFLKDREDRYQGCNLA
FETASGRSRAEILGQRMEQVLRSENYDRHRAEEALVLREGRTRVWEAMEHDVSGRHKLISITASPYREPGDPTVRGLIGV
VSDITRRQRAEDALRASVVELTASNRELQHFAEIAAHDLQEPVRAVVSHCQLLERHLGDALDGPARDHMIFAITGARRMR
DLIQDLLIYARAGRDGQFHEVVDTADLAREVVSALADEIAASGATIRIDPLPSVLGRRRDLFDVMVNLFDNALKFRRPDV
VPEVTLKAAETLDASGLWCFTLSDNGIGLDPVHGAQIFALFRRLHGADRYPGTGVGLAICRRIVEQHGGTIFVESAPGVG
TRFHFTLPAAPSEEPDRLVGRGTRSAPRPVIDGGG
>Mature_514_residues
SGEPVGLALQRLYDLTRLVSDWIWETDSAGLLTYASDRVFEILGYPARALIGHPFAELAASGDPPFFKSDPRPFREQRFE
ARHSDGSPRVLLISAVPLFDAQSGDRQGWRGAAKDITQSERADAEMDRQRRFQQTLLDAIPLPVFLKDREDRYQGCNLAF
ETASGRSRAEILGQRMEQVLRSENYDRHRAEEALVLREGRTRVWEAMEHDVSGRHKLISITASPYREPGDPTVRGLIGVV
SDITRRQRAEDALRASVVELTASNRELQHFAEIAAHDLQEPVRAVVSHCQLLERHLGDALDGPARDHMIFAITGARRMRD
LIQDLLIYARAGRDGQFHEVVDTADLAREVVSALADEIAASGATIRIDPLPSVLGRRRDLFDVMVNLFDNALKFRRPDVV
PEVTLKAAETLDASGLWCFTLSDNGIGLDPVHGAQIFALFRRLHGADRYPGTGVGLAICRRIVEQHGGTIFVESAPGVGT
RFHFTLPAAPSEEPDRLVGRGTRSAPRPVIDGGG

Specific function: Photoreceptor which exists in two forms that are reversibly interconvertible by light:the R form that absorbs maximally in the red region of the spectrum and the FR form that absorbs maximally in the far-red region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=392, Percent_Identity=27.0408163265306, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788713, Length=388, Percent_Identity=22.1649484536082, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788549, Length=390, Percent_Identity=24.6153846153846, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786600, Length=283, Percent_Identity=26.1484098939929, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790346, Length=220, Percent_Identity=27.7272727272727, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786912, Length=241, Percent_Identity=31.9502074688797, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87081816, Length=230, Percent_Identity=26.0869565217391, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003018
- InterPro:   IPR013654
- InterPro:   IPR016132
- InterPro:   IPR001294
- InterPro:   IPR013515
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF01590 GAF; PF02518 HATPase_c; PF00512 HisKA; PF08446 PAS_2; PF00360 Phytochrome [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57121; Mature: 56990

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGEPVGLALQRLYDLTRLVSDWIWETDSAGLLTYASDRVFEILGYPARALIGHPFAELA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHCCCHHHHHCCCHHHHH
ASGDPPFFKSDPRPFREQRFEARHSDGSPRVLLISAVPLFDAQSGDRQGWRGAAKDITQS
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
ERADAEMDRQRRFQQTLLDAIPLPVFLKDREDRYQGCNLAFETASGRSRAEILGQRMEQV
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHH
LRSENYDRHRAEEALVLREGRTRVWEAMEHDVSGRHKLISITASPYREPGDPTVRGLIGV
HHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
VSDITRRQRAEDALRASVVELTASNRELQHFAEIAAHDLQEPVRAVVSHCQLLERHLGDA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
LDGPARDHMIFAITGARRMRDLIQDLLIYARAGRDGQFHEVVDTADLAREVVSALADEIA
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASGATIRIDPLPSVLGRRRDLFDVMVNLFDNALKFRRPDVVPEVTLKAAETLDASGLWCF
HCCCEEEECCCHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEE
TLSDNGIGLDPVHGAQIFALFRRLHGADRYPGTGVGLAICRRIVEQHGGTIFVESAPGVG
EECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
TRFHFTLPAAPSEEPDRLVGRGTRSAPRPVIDGGG
CEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SGEPVGLALQRLYDLTRLVSDWIWETDSAGLLTYASDRVFEILGYPARALIGHPFAELA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHCCCHHHHHCCCHHHHH
ASGDPPFFKSDPRPFREQRFEARHSDGSPRVLLISAVPLFDAQSGDRQGWRGAAKDITQS
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
ERADAEMDRQRRFQQTLLDAIPLPVFLKDREDRYQGCNLAFETASGRSRAEILGQRMEQV
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHH
LRSENYDRHRAEEALVLREGRTRVWEAMEHDVSGRHKLISITASPYREPGDPTVRGLIGV
HHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHH
VSDITRRQRAEDALRASVVELTASNRELQHFAEIAAHDLQEPVRAVVSHCQLLERHLGDA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
LDGPARDHMIFAITGARRMRDLIQDLLIYARAGRDGQFHEVVDTADLAREVVSALADEIA
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASGATIRIDPLPSVLGRRRDLFDVMVNLFDNALKFRRPDVVPEVTLKAAETLDASGLWCF
HCCCEEEECCCHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEE
TLSDNGIGLDPVHGAQIFALFRRLHGADRYPGTGVGLAICRRIVEQHGGTIFVESAPGVG
EECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
TRFHFTLPAAPSEEPDRLVGRGTRSAPRPVIDGGG
CEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]