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The map label for this gene is rlmI [H]

Identifier: 83594662

GI number: 83594662

Start: 3845680

End: 3846930

Strand: Direct

Name: rlmI [H]

Synonym: Rru_A3333

Alternate gene names: 83594662

Gene position: 3845680-3846930 (Clockwise)

Preceding gene: 83594658

Following gene: 83594663

Centisome position: 88.35

GC content: 66.99

Gene sequence:

>1251_bases
ATGAGAAAATGCCCTATCCTGGGGCCTCCTCCCCCTTTCGGAATCGTCATGACAGAGCCAAATTCGCCGTCTCTCCCCGA
AATCCGCCTTCTCGCCGGCCACTCGAAGCGGCTCCGCCAGGGCCATCCCTGGGTTTTCTCCAACGAGATCGCCATGACCC
CGGAGGCCAAGGCCATGACTCCGGGAGCGCTTGTCACCTTGCGCGATGCCGGGGATGAGCCGCTGGCCATCGCCACGTTT
AATCCGCATTCGCTGATCGCCGCGCGGGTTCTTGATCGCGATCTTGGCGCCAGCATCACCAAGGAATGGGTCTTCGTCCG
GCTTCAGCGGGCCTTGCGCCTGCGCGACACGCTGTTTGACCAACCCTATTACCGGCTCGTCCATGGCGAGGCCGACGGCC
TGCCCGGGCTGGTCGTCGACCGTTTGGGCGATGTCATCGCCGTCCAGGCCAACAGCGCCGGGATGGATCTGCTGACGCCA
CTGATTCTCGACGCCATCGAAAACCTGCTGGCGCCGCGCGCCATCGTGCTGATCAACGACGCGCCGGTGCGCCTACTCGA
AGGGCTGACCCAGGAAACCGCCCTGGCCCGGGGCCAGATCGACGGCCCGGTGCCGGTGATCGAAAACGGCTTTTCCTATC
TGGCCGACCTGCAGGAAGGCCAGAAGACCGGCTGGTTCTTCGATCAGCGGCCCAATCGCGCCTTCGTCGCCGACCTCGCC
CGCGGCCGCTCGGTGCTTGATGTCTATAGCTATGCCGGGGGCTTCGGGCTGCTGGCGCTGGCGCGCGGCGCCACCTCGGC
CACCCTGGTCGACCGCTCCGACCAGGGCCTGCTGCTGGCCCAACAGGCCGCCGCCACCGCCGGACTCGGCGGGGCGCTGA
CCACCCACAAGGCCGAGGGTTTCGCCTACCTCGAGCAAGCAGAGCATGAGGGCAAGCGTTTTGGCGTCGTCGTCTGCGAT
CCGCCGGCCTTCGCCAAGACCCGCAAGGATCAGGCCTCGGGCGCCAAGGGCTATCGCAAGGTCGCCCGCCTCGCCGCCGC
CCTGGTCGAACCCGGCGGCTTTCTGTTCGTCGCCTCGTGCAGCCATCACATGCCGATCGACCGCTTCCAGGACGAAACCG
CCCATGGCATCGCCCAGGCCGGGCGGACGGGGCGGATCTTGCGCTCGGGCGGCGCCGGTCCCGACCATCCGGTCCATCCC
GATCTGGCCGAATCGGCCTATCTCAAGACCCTGACCTGGGCGATCGACTAA

Upstream 100 bases:

>100_bases
TTCCCGTCCGCCATTGATTTAGATCAAATCCAGATCACAATTGCGACCCAAGAATTTCGCAAAACCAATCACGTCCGCGC
ATTTCCCGACCAGTTCCAAC

Downstream 100 bases:

>100_bases
GCCTGAGCGTCTGATCGGCCTCGCCGATCCTTCCGCCGGCTCCCTCTTTCAAAGGGGGGCCGGCGGGAGCGCGCTTTTCT
TCTTGTCCCGCAAGTGGCGT

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MRKCPILGPPPPFGIVMTEPNSPSLPEIRLLAGHSKRLRQGHPWVFSNEIAMTPEAKAMTPGALVTLRDAGDEPLAIATF
NPHSLIAARVLDRDLGASITKEWVFVRLQRALRLRDTLFDQPYYRLVHGEADGLPGLVVDRLGDVIAVQANSAGMDLLTP
LILDAIENLLAPRAIVLINDAPVRLLEGLTQETALARGQIDGPVPVIENGFSYLADLQEGQKTGWFFDQRPNRAFVADLA
RGRSVLDVYSYAGGFGLLALARGATSATLVDRSDQGLLLAQQAAATAGLGGALTTHKAEGFAYLEQAEHEGKRFGVVVCD
PPAFAKTRKDQASGAKGYRKVARLAAALVEPGGFLFVASCSHHMPIDRFQDETAHGIAQAGRTGRILRSGGAGPDHPVHP
DLAESAYLKTLTWAID

Sequences:

>Translated_416_residues
MRKCPILGPPPPFGIVMTEPNSPSLPEIRLLAGHSKRLRQGHPWVFSNEIAMTPEAKAMTPGALVTLRDAGDEPLAIATF
NPHSLIAARVLDRDLGASITKEWVFVRLQRALRLRDTLFDQPYYRLVHGEADGLPGLVVDRLGDVIAVQANSAGMDLLTP
LILDAIENLLAPRAIVLINDAPVRLLEGLTQETALARGQIDGPVPVIENGFSYLADLQEGQKTGWFFDQRPNRAFVADLA
RGRSVLDVYSYAGGFGLLALARGATSATLVDRSDQGLLLAQQAAATAGLGGALTTHKAEGFAYLEQAEHEGKRFGVVVCD
PPAFAKTRKDQASGAKGYRKVARLAAALVEPGGFLFVASCSHHMPIDRFQDETAHGIAQAGRTGRILRSGGAGPDHPVHP
DLAESAYLKTLTWAID
>Mature_416_residues
MRKCPILGPPPPFGIVMTEPNSPSLPEIRLLAGHSKRLRQGHPWVFSNEIAMTPEAKAMTPGALVTLRDAGDEPLAIATF
NPHSLIAARVLDRDLGASITKEWVFVRLQRALRLRDTLFDQPYYRLVHGEADGLPGLVVDRLGDVIAVQANSAGMDLLTP
LILDAIENLLAPRAIVLINDAPVRLLEGLTQETALARGQIDGPVPVIENGFSYLADLQEGQKTGWFFDQRPNRAFVADLA
RGRSVLDVYSYAGGFGLLALARGATSATLVDRSDQGLLLAQQAAATAGLGGALTTHKAEGFAYLEQAEHEGKRFGVVVCD
PPAFAKTRKDQASGAKGYRKVARLAAALVEPGGFLFVASCSHHMPIDRFQDETAHGIAQAGRTGRILRSGGAGPDHPVHP
DLAESAYLKTLTWAID

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=393, Percent_Identity=33.8422391857506, Blast_Score=194, Evalue=6e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44632; Mature: 44632

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKCPILGPPPPFGIVMTEPNSPSLPEIRLLAGHSKRLRQGHPWVFSNEIAMTPEAKAMT
CCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHCCCCEEEECCEEECCCCCCCC
PGALVTLRDAGDEPLAIATFNPHSLIAARVLDRDLGASITKEWVFVRLQRALRLRDTLFD
CCEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
QPYYRLVHGEADGLPGLVVDRLGDVIAVQANSAGMDLLTPLILDAIENLLAPRAIVLIND
CCCEEEEECCCCCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECC
APVRLLEGLTQETALARGQIDGPVPVIENGFSYLADLQEGQKTGWFFDQRPNRAFVADLA
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHH
RGRSVLDVYSYAGGFGLLALARGATSATLVDRSDQGLLLAQQAAATAGLGGALTTHKAEG
CCCHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHHCCCCCCCCCCCCCC
FAYLEQAEHEGKRFGVVVCDPPAFAKTRKDQASGAKGYRKVARLAAALVEPGGFLFVASC
HHHHHHHHCCCCEEEEEEECCCCHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEEECC
SHHMPIDRFQDETAHGIAQAGRTGRILRSGGAGPDHPVHPDLAESAYLKTLTWAID
CCCCCHHHHCCHHHHHHHHCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHEEEECC
>Mature Secondary Structure
MRKCPILGPPPPFGIVMTEPNSPSLPEIRLLAGHSKRLRQGHPWVFSNEIAMTPEAKAMT
CCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHCCCCEEEECCEEECCCCCCCC
PGALVTLRDAGDEPLAIATFNPHSLIAARVLDRDLGASITKEWVFVRLQRALRLRDTLFD
CCEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
QPYYRLVHGEADGLPGLVVDRLGDVIAVQANSAGMDLLTPLILDAIENLLAPRAIVLIND
CCCEEEEECCCCCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECC
APVRLLEGLTQETALARGQIDGPVPVIENGFSYLADLQEGQKTGWFFDQRPNRAFVADLA
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHH
RGRSVLDVYSYAGGFGLLALARGATSATLVDRSDQGLLLAQQAAATAGLGGALTTHKAEG
CCCHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHHCCCCCCCCCCCCCC
FAYLEQAEHEGKRFGVVVCDPPAFAKTRKDQASGAKGYRKVARLAAALVEPGGFLFVASC
HHHHHHHHCCCCEEEEEEECCCCHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEEECC
SHHMPIDRFQDETAHGIAQAGRTGRILRSGGAGPDHPVHPDLAESAYLKTLTWAID
CCCCCHHHHCCHHHHHHHHCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA