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The map label for this gene is rlmN

Identifier: 83594658

GI number: 83594658

Start: 3840540

End: 3841826

Strand: Direct

Name: rlmN

Synonym: Rru_A3329

Alternate gene names: 83594658

Gene position: 3840540-3841826 (Clockwise)

Preceding gene: 83594656

Following gene: 83594662

Centisome position: 88.23

GC content: 63.95

Gene sequence:

>1287_bases
ATGCCCTTACACCGGGTGGAAGCATTGGGAGAACCCCAGGACAGGACCGGAAAAACCTTTTCCGGCAGAACGAACGGACC
TATATCAAGTCCCTTAACGGATACAGAGCGACGCATGTCTATACCGCAAAACCCCGCGCCGGTGAACCTTGTCGGCCTCT
CAAGAGAAGAGATTGCCGCCCTTCTACGCGATATGGGCGAAAAACCCTTCAGAGCAAAGCAGCTCTGGCACTGGGTCTAC
CACCGCGGCGAAACCGACTTTTCGGCCATGACCACCCTTGGAACGCCCTTGCGGGCGAAGCTGGCGGAAACCTGCGTGGT
CGCCCGCCCGCACGTTGTTCGAGAACAGCGCTCCGAGGATGGAACGCGTAAGTGGCTACTTCGGTTCCCTGACGGAAACG
AGGCCGAAACCGTTTATATTCCTGAAGACGATCGGGGCGCTCTCTGTGTGTCCTCGCAGGTCGGCTGCACGCTGACCTGC
CGGTTCTGTCATACGGGCACCCAGTTGCTGGTCCGCAACCTGACCGCCCACGAGATCGTCGGCCAGTTCATGGCCGCGCG
CGACGCCTATGGCGAATGGCCGTCGCCGACCGACGAGTCGCGCCAACTGTCCAACATCGTGCTGATGGGCATGGGCGAAC
CGCTCTACAACTACGACAACGTCGCCAAGGCGATCGGCATCCTGCTTGATAACGAGGGGATCGCCGTCTCGCGGCGGCGG
ATCACGCTTTCGACCTCGGGGGTGGTGCCGATGATCCGGCGCTGCGGCGCCGAGCTGGGCGTCAATCTGGCGGTCAGCCT
GCATGCGGCGCGCGACGAGATCCGCGACGAGATCATGCCGATCAACCGCAAATATCCGCTGGCCGAGTTGATGGCGGCCT
GCCGCGAGTATCCGGGCGCCAGCAACGCCCGGCGCATCACCTTTGAATACGTCATGCTCAAGGGCGTGAACGATTCCGAA
GCCGACGCCCGCGCCCTGATCAAGCTGGTCGAGGGGGTTCCCTGCAAGTTCAACCTGATCCCCTTCAATCCCTGGCCGGG
CTCGGGCTTCGAATGCCCGCCGATCCGCCATATCGAGCGCTTCGCCAACATCTTGTTCGAGGCCGGCTACACGGCGCCGA
TCCGCATGCCGCGCGGCCGCGACATCCTGGCCGCCTGCGGTCAGCTGCGCAGCGACTCCTTGCGCGAACGCGCCAGCCTG
CATAAGGCCCGGCTGGCGGCCGGAGTGGCCGACGATCACGCCCCGGCCGCCGCTCCCTTGGCGGATACTCCGGGCGCCGT
GGCCTAA

Upstream 100 bases:

>100_bases
GACGAATGTCTTGGTAATTAAGTCCGCACTTCCAACTCCTCCAGGTCCCGGAGCAAAGAAAGCGCTGTTTTTTGCCTGAT
CGATCGATTCATTGCAAAAA

Downstream 100 bases:

>100_bases
CCCTCCGCCCCCGCCCCGCCTCCACGAAAAACCGCCCGGCTCCTTCGCAAGGAACCGGGCGGTTTTTTTATCGGGATCGC
CAAGAGCGGGAAGACCGCCT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MPLHRVEALGEPQDRTGKTFSGRTNGPISSPLTDTERRMSIPQNPAPVNLVGLSREEIAALLRDMGEKPFRAKQLWHWVY
HRGETDFSAMTTLGTPLRAKLAETCVVARPHVVREQRSEDGTRKWLLRFPDGNEAETVYIPEDDRGALCVSSQVGCTLTC
RFCHTGTQLLVRNLTAHEIVGQFMAARDAYGEWPSPTDESRQLSNIVLMGMGEPLYNYDNVAKAIGILLDNEGIAVSRRR
ITLSTSGVVPMIRRCGAELGVNLAVSLHAARDEIRDEIMPINRKYPLAELMAACREYPGASNARRITFEYVMLKGVNDSE
ADARALIKLVEGVPCKFNLIPFNPWPGSGFECPPIRHIERFANILFEAGYTAPIRMPRGRDILAACGQLRSDSLRERASL
HKARLAAGVADDHAPAAAPLADTPGAVA

Sequences:

>Translated_428_residues
MPLHRVEALGEPQDRTGKTFSGRTNGPISSPLTDTERRMSIPQNPAPVNLVGLSREEIAALLRDMGEKPFRAKQLWHWVY
HRGETDFSAMTTLGTPLRAKLAETCVVARPHVVREQRSEDGTRKWLLRFPDGNEAETVYIPEDDRGALCVSSQVGCTLTC
RFCHTGTQLLVRNLTAHEIVGQFMAARDAYGEWPSPTDESRQLSNIVLMGMGEPLYNYDNVAKAIGILLDNEGIAVSRRR
ITLSTSGVVPMIRRCGAELGVNLAVSLHAARDEIRDEIMPINRKYPLAELMAACREYPGASNARRITFEYVMLKGVNDSE
ADARALIKLVEGVPCKFNLIPFNPWPGSGFECPPIRHIERFANILFEAGYTAPIRMPRGRDILAACGQLRSDSLRERASL
HKARLAAGVADDHAPAAAPLADTPGAVA
>Mature_427_residues
PLHRVEALGEPQDRTGKTFSGRTNGPISSPLTDTERRMSIPQNPAPVNLVGLSREEIAALLRDMGEKPFRAKQLWHWVYH
RGETDFSAMTTLGTPLRAKLAETCVVARPHVVREQRSEDGTRKWLLRFPDGNEAETVYIPEDDRGALCVSSQVGCTLTCR
FCHTGTQLLVRNLTAHEIVGQFMAARDAYGEWPSPTDESRQLSNIVLMGMGEPLYNYDNVAKAIGILLDNEGIAVSRRRI
TLSTSGVVPMIRRCGAELGVNLAVSLHAARDEIRDEIMPINRKYPLAELMAACREYPGASNARRITFEYVMLKGVNDSEA
DARALIKLVEGVPCKFNLIPFNPWPGSGFECPPIRHIERFANILFEAGYTAPIRMPRGRDILAACGQLRSDSLRERASLH
KARLAAGVADDHAPAAAPLADTPGAVA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=366, Percent_Identity=47.2677595628415, Blast_Score=338, Evalue=4e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_RHORT (Q2RP22)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428410.1
- STRING:   Q2RP22
- GeneID:   3836781
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3329
- NMPDR:   fig|1085.1.peg.2166
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   Q2RP22
- BioCyc:   RRUB269796:RRU_A3329-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 47159; Mature: 47027

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHRVEALGEPQDRTGKTFSGRTNGPISSPLTDTERRMSIPQNPAPVNLVGLSREEIAA
CCCHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEECCHHHHHH
LLRDMGEKPFRAKQLWHWVYHRGETDFSAMTTLGTPLRAKLAETCVVARPHVVREQRSED
HHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCC
GTRKWLLRFPDGNEAETVYIPEDDRGALCVSSQVGCTLTCRFCHTGTQLLVRNLTAHEIV
CCEEEEEECCCCCCCCEEECCCCCCCCEEEECCCCEEEEEEEHHCCHHHHHHHHHHHHHH
GQFMAARDAYGEWPSPTDESRQLSNIVLMGMGEPLYNYDNVAKAIGILLDNEGIAVSRRR
HHHHHHHHHCCCCCCCCCHHHHHCCEEEEECCCCCCCHHHHHHHHHEEECCCCCEEEEEE
ITLSTSGVVPMIRRCGAELGVNLAVSLHAARDEIRDEIMPINRKYPLAELMAACREYPGA
EEEECCCHHHHHHHCCHHHCCEEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
SNARRITFEYVMLKGVNDSEADARALIKLVEGVPCKFNLIPFNPWPGSGFECPPIRHIER
CCCEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHH
FANILFEAGYTAPIRMPRGRDILAACGQLRSDSLRERASLHKARLAAGVADDHAPAAAPL
HHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ADTPGAVA
CCCCCCCC
>Mature Secondary Structure 
PLHRVEALGEPQDRTGKTFSGRTNGPISSPLTDTERRMSIPQNPAPVNLVGLSREEIAA
CCHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEECCHHHHHH
LLRDMGEKPFRAKQLWHWVYHRGETDFSAMTTLGTPLRAKLAETCVVARPHVVREQRSED
HHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCC
GTRKWLLRFPDGNEAETVYIPEDDRGALCVSSQVGCTLTCRFCHTGTQLLVRNLTAHEIV
CCEEEEEECCCCCCCCEEECCCCCCCCEEEECCCCEEEEEEEHHCCHHHHHHHHHHHHHH
GQFMAARDAYGEWPSPTDESRQLSNIVLMGMGEPLYNYDNVAKAIGILLDNEGIAVSRRR
HHHHHHHHHCCCCCCCCCHHHHHCCEEEEECCCCCCCHHHHHHHHHEEECCCCCEEEEEE
ITLSTSGVVPMIRRCGAELGVNLAVSLHAARDEIRDEIMPINRKYPLAELMAACREYPGA
EEEECCCHHHHHHHCCHHHCCEEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
SNARRITFEYVMLKGVNDSEADARALIKLVEGVPCKFNLIPFNPWPGSGFECPPIRHIER
CCCEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHH
FANILFEAGYTAPIRMPRGRDILAACGQLRSDSLRERASLHKARLAAGVADDHAPAAAPL
HHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ADTPGAVA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA