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The map label for this gene is oprM [H]

Identifier: 83594567

GI number: 83594567

Start: 3733988

End: 3735373

Strand: Direct

Name: oprM [H]

Synonym: Rru_A3237

Alternate gene names: 83594567

Gene position: 3733988-3735373 (Clockwise)

Preceding gene: 83594566

Following gene: 83594571

Centisome position: 85.78

GC content: 71.07

Gene sequence:

>1386_bases
ATGCGTCCCGCCGTTTTCCCCGCCGCCCTGGCCCTTTTTCTGGGCGGCTGCTCGCTGGTTCCCGACAGCGAGCGGCCAGC
GCTTGCCATGCCGGCGGCCTTCGGCGATGCCGCTCCGGCGCCCGCCGCCACGCCGTTGGCCGATGCCACATGGTGGCGGC
GCTTCGGCTCGCCCGAGCTTTCCGCGCTGATGAGCGACGCGATGACCGGCAATCAGGATCTGGAAGCCGCCTTCCGCCGC
GTGGCCCAGGCCCGGGCCAGTCTGGCGGGAACCCGCGCCGGCTGGTTCCCGACGCTCAGCGCCAGCAGCAATGCCAACCG
CAGCTTGCACAACACGGCGCGCACCGCCGCCGCCACCGAAAAGACCACCTATGATGTCGGCGGCGATCTGTCGTGGGAGG
TCGATCTGTTTGGCAAGACCCGCGCCAGCGTGCTGTCGGCCGAGGCGACCGCCCAGTCCCAGGCCCTGGCCCGCGACGCC
CTGGCGCTGTCCATCCAGGGCGAGGTGGCCACCGCCTATGTCACCGCCCTGGCCGCCAAGGACCGGCTGGCGATCGCCCG
GGAAAATCTGGCGACGGCCCGCGAAATCCTGGCGCTGATCGAAACCCAGGTGAGCATCGGATCGCAAGCGCCGCTCGAAC
AGGCCCAGCAACGCTCGACCGTCGCCGGGATCGAGGCCAGCCTGCCGCAGTTGGAAGCCGATCTGGCGGCGGCGCAGACC
TCCCTCGCCGTGCTGTTGGGCCGCGCCCCCCAGGGCTTCCGCATCCAGGCCCCGGGGCTGAACGCCCTGGCCTTGCCGGC
CATCGCGCCCGGCCAACCCTCCGACCTTCTCGAACGCCGCCCCGATATCCGCGAGGCCGAGGCCACCCTGATCGCCGCCA
ATGCCGATATCGGCGCCGCCCGCTCCGCCTTCTTTCCCACCCTGACCCTGTCGGCCAGCGCCTCGCTAAGCGGCGCCTTG
AGCGGCGGAACGACAACGGCGGGGTCGCTGGCCGGCAACCTGTTGGGCACCATCTTCGATGCCGGCAAGCGCGAGGCCGA
TCTGGAAGTCGCCCGCCAGCGCTGGGCCGAACTGGCCGCCACTTATCGCACCACGGTGCTGACGGCCTTAAAGGAGGCCG
AGGACGCCCTGGTGACCGAAGACACCGGGGCGCGGCGCGAGGTGGCCCTGGCCGAGGCGGTGGTCCAATCGACCGAGGCG
CTGCGCATCGCCCGCACCCAGTATCAGGTGGGCGACGGCGATTTCCTGGCCGTGCTCACCGCCCAGCAAACCGTTCTGTC
CTCGCGCGATTCCCTGGCCCAGGCCCGGGGGGACCGCTTCCAGTCGGCGATCGATCTGGTCAAGGCCCTGGGCAGCGGCT
GGCGCGAACCTACAGCTTCCCCTTGA

Upstream 100 bases:

>100_bases
CGCCTTTCTGACCGGACTGCTGTTTGGCTATCTGCCCGCCCGCAAGGCCGCCCGCCTTGATCCCGTCGTCGCCCTGTCCG
CCGAATAGAGGACCCTTGCC

Downstream 100 bases:

>100_bases
TCTCCGACTTGGAGTAATTGCTGAAATGCCCCTCGCGGGCGGCCTGCTGGCGGAACTTGGCAAGCTTGCCCGACGAGGTG
GACGGCGCCGCCGTCCGCCC

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRPAVFPAALALFLGGCSLVPDSERPALAMPAAFGDAAPAPAATPLADATWWRRFGSPELSALMSDAMTGNQDLEAAFRR
VAQARASLAGTRAGWFPTLSASSNANRSLHNTARTAAATEKTTYDVGGDLSWEVDLFGKTRASVLSAEATAQSQALARDA
LALSIQGEVATAYVTALAAKDRLAIARENLATAREILALIETQVSIGSQAPLEQAQQRSTVAGIEASLPQLEADLAAAQT
SLAVLLGRAPQGFRIQAPGLNALALPAIAPGQPSDLLERRPDIREAEATLIAANADIGAARSAFFPTLTLSASASLSGAL
SGGTTTAGSLAGNLLGTIFDAGKREADLEVARQRWAELAATYRTTVLTALKEAEDALVTEDTGARREVALAEAVVQSTEA
LRIARTQYQVGDGDFLAVLTAQQTVLSSRDSLAQARGDRFQSAIDLVKALGSGWREPTASP

Sequences:

>Translated_461_residues
MRPAVFPAALALFLGGCSLVPDSERPALAMPAAFGDAAPAPAATPLADATWWRRFGSPELSALMSDAMTGNQDLEAAFRR
VAQARASLAGTRAGWFPTLSASSNANRSLHNTARTAAATEKTTYDVGGDLSWEVDLFGKTRASVLSAEATAQSQALARDA
LALSIQGEVATAYVTALAAKDRLAIARENLATAREILALIETQVSIGSQAPLEQAQQRSTVAGIEASLPQLEADLAAAQT
SLAVLLGRAPQGFRIQAPGLNALALPAIAPGQPSDLLERRPDIREAEATLIAANADIGAARSAFFPTLTLSASASLSGAL
SGGTTTAGSLAGNLLGTIFDAGKREADLEVARQRWAELAATYRTTVLTALKEAEDALVTEDTGARREVALAEAVVQSTEA
LRIARTQYQVGDGDFLAVLTAQQTVLSSRDSLAQARGDRFQSAIDLVKALGSGWREPTASP
>Mature_461_residues
MRPAVFPAALALFLGGCSLVPDSERPALAMPAAFGDAAPAPAATPLADATWWRRFGSPELSALMSDAMTGNQDLEAAFRR
VAQARASLAGTRAGWFPTLSASSNANRSLHNTARTAAATEKTTYDVGGDLSWEVDLFGKTRASVLSAEATAQSQALARDA
LALSIQGEVATAYVTALAAKDRLAIARENLATAREILALIETQVSIGSQAPLEQAQQRSTVAGIEASLPQLEADLAAAQT
SLAVLLGRAPQGFRIQAPGLNALALPAIAPGQPSDLLERRPDIREAEATLIAANADIGAARSAFFPTLTLSASASLSGAL
SGGTTTAGSLAGNLLGTIFDAGKREADLEVARQRWAELAATYRTTVLTALKEAEDALVTEDTGARREVALAEAVVQSTEA
LRIARTQYQVGDGDFLAVLTAQQTVLSSRDSLAQARGDRFQSAIDLVKALGSGWREPTASP

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=463, Percent_Identity=30.8855291576674, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI2367348, Length=475, Percent_Identity=24, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 47874; Mature: 47874

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPAVFPAALALFLGGCSLVPDSERPALAMPAAFGDAAPAPAATPLADATWWRRFGSPEL
CCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHCCCHHH
SALMSDAMTGNQDLEAAFRRVAQARASLAGTRAGWFPTLSASSNANRSLHNTARTAAATE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
KTTYDVGGDLSWEVDLFGKTRASVLSAEATAQSQALARDALALSIQGEVATAYVTALAAK
CCEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHH
DRLAIARENLATAREILALIETQVSIGSQAPLEQAQQRSTVAGIEASLPQLEADLAAAQT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
SLAVLLGRAPQGFRIQAPGLNALALPAIAPGQPSDLLERRPDIREAEATLIAANADIGAA
HHHHHHCCCCCCCEEECCCCCEEECCCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCCCH
RSAFFPTLTLSASASLSGALSGGTTTAGSLAGNLLGTIFDAGKREADLEVARQRWAELAA
HHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TYRTTVLTALKEAEDALVTEDTGARREVALAEAVVQSTEALRIARTQYQVGDGDFLAVLT
HHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEE
AQQTVLSSRDSLAQARGDRFQSAIDLVKALGSGWREPTASP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRPAVFPAALALFLGGCSLVPDSERPALAMPAAFGDAAPAPAATPLADATWWRRFGSPEL
CCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHCCCHHH
SALMSDAMTGNQDLEAAFRRVAQARASLAGTRAGWFPTLSASSNANRSLHNTARTAAATE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
KTTYDVGGDLSWEVDLFGKTRASVLSAEATAQSQALARDALALSIQGEVATAYVTALAAK
CCEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHH
DRLAIARENLATAREILALIETQVSIGSQAPLEQAQQRSTVAGIEASLPQLEADLAAAQT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
SLAVLLGRAPQGFRIQAPGLNALALPAIAPGQPSDLLERRPDIREAEATLIAANADIGAA
HHHHHHCCCCCCCEEECCCCCEEECCCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCCCH
RSAFFPTLTLSASASLSGALSGGTTTAGSLAGNLLGTIFDAGKREADLEVARQRWAELAA
HHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TYRTTVLTALKEAEDALVTEDTGARREVALAEAVVQSTEALRIARTQYQVGDGDFLAVLT
HHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEE
AQQTVLSSRDSLAQARGDRFQSAIDLVKALGSGWREPTASP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]