Click here to switch to the map view.

The map label for this gene is ydaM [H]

Identifier: 83594462

GI number: 83594462

Start: 3611580

End: 3613061

Strand: Direct

Name: ydaM [H]

Synonym: Rru_A3132

Alternate gene names: 83594462

Gene position: 3611580-3613061 (Clockwise)

Preceding gene: 83594458

Following gene: 83594463

Centisome position: 82.97

GC content: 64.24

Gene sequence:

>1482_bases
ATGTCCGCCGATCGCCTCCTCACCTGCCTCGCGGAGTGCAGTACGCGCCTTGATGGAGCGGACACCTGGCCGGTATTGAT
GAAGCAGATCCTGGCCTTGGTCGGGGAGGCGAGCGGAGCGACCAGGACCTGGGTTTTCCAGACGATCACGCTAACCGATC
AGCATGTCATCCAAGATCACCTGTTCGAACATATCAGCGAGGACTGCTGGCAACTTCAGCTGCGCAAGCGCTTCCGGCTT
TTCCGCACCGCCTTGTCCGCGCCCGACTATCATGATCTGGTCCGCGACCGTCAAAGGGGCCTGCCGTCGCTGCTGGTGAC
GGCGGAGATTGCCCCGGGCTTCCTCAAGGCCTCGCTCGAACGCCAAAAGACCGCCGCCATGCTGAGCGCGCCGATCCTGG
TCGAGGGCCAGTGGTGGGGAACCATCGGCCTGGACGATTGTCAGGGCGGGGCGGACTGGAACGCGACCACCATCAAATTC
CTCCAGACTATCGCCGGCTTGATCGCCGGTTCGATCCACCGCGAGCGCCAAAGGGTGCGCAGCCAGCAAGTCGCCGTCAT
GGAGCGCACCACCGGGGGCGGTCTTTGGGAGCTTGACGTTCTGCGCGGCGATGTCTGGTGTTCGGAAACGCTGTTCCATC
TGCTTGGCTATCCCCCGCCCTGCGCCCGCCTGACCATCCGCCGCCTGCTGCGCCACATCCTGCCCGAGGACCGGGCGCTT
GTGATGGCCTCGGCGCGCCGCTGCATGCGCGAGGGCCTGCCGGCTTTCCGTCTGGATGTCCGCGCTTGGCGCCACGATAG
AACGCTTTTGTGGTGGGAATTGATCATCGATGTTACCGAAACCCGCCCCCTCGACGGCTTTCCCTTGCGGATGACCGGCA
TCGTCCTCGAAGTCAACGACCGCAAGACCCAGGAACTGGAACTGGCGCGGACCGCGGCGACCGATCCTCTGACCGGCTTG
GCCAACCGCCGCTCCTTCGAAGCCCTGGCGACCCGCCTGCTGGAAAACAAACCGGGATCGGGCACGCTGCTTGTGATCGA
TATCGACCTGTTCAAGCATATCAACGACTGCTTCGGCCATGCCGCCGGCGATGAGGTCTTGCGCCACCTCGCCATCGTCG
GCCGCTCGGTGTTGCGCGATGGCGATCTGATCGGTCGGCTCGGCGGCGAGGAATTCGCCGTTCTTTTGCAAATGGACGGG
CCCGAAGCCTATGCGGTGGCCGACCGCCTGTGTCAGGCGGTGCGCGAAATGGCGGTCACCGCGCCGTCTTGGGAACACTC
CTCGGTGGTGGTGAAGATCACCATCAGCATCGGCATCGCCCACCCCTGCCCCTTCGAGGATCCGAAGTTCGCCCTGAGCG
AAGCTCTGCGGCGGGCCGACCGGGCGCTTTACGGGGCCAAGAAGCGGGGACGCAATCAGATCTGCCACGATACGGAAGGC
CGACAGTCCGCCATTTCCCGGGACAACCGGGCAAAAAAATAG

Upstream 100 bases:

>100_bases
AAATTCATTATAAACTGACCAAAAATGAGTCAGTTCATTTAAAAATATTTTCCTCTTCCGTTTTTTGAATGCCACCTTCC
CCTTGCATTCCGAGGAAACC

Downstream 100 bases:

>100_bases
TTCTATGGGGCAACGACCCGACAGGCCCGCTCCTTTTCAGCCGTCAAGGGAAACCGCCCGGTGTTTTCGATCTTCTTCCT
GATCGCGCTGATGGCGATCG

Product: diguanylate cyclase with GAF sensor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSADRLLTCLAECSTRLDGADTWPVLMKQILALVGEASGATRTWVFQTITLTDQHVIQDHLFEHISEDCWQLQLRKRFRL
FRTALSAPDYHDLVRDRQRGLPSLLVTAEIAPGFLKASLERQKTAAMLSAPILVEGQWWGTIGLDDCQGGADWNATTIKF
LQTIAGLIAGSIHRERQRVRSQQVAVMERTTGGGLWELDVLRGDVWCSETLFHLLGYPPPCARLTIRRLLRHILPEDRAL
VMASARRCMREGLPAFRLDVRAWRHDRTLLWWELIIDVTETRPLDGFPLRMTGIVLEVNDRKTQELELARTAATDPLTGL
ANRRSFEALATRLLENKPGSGTLLVIDIDLFKHINDCFGHAAGDEVLRHLAIVGRSVLRDGDLIGRLGGEEFAVLLQMDG
PEAYAVADRLCQAVREMAVTAPSWEHSSVVVKITISIGIAHPCPFEDPKFALSEALRRADRALYGAKKRGRNQICHDTEG
RQSAISRDNRAKK

Sequences:

>Translated_493_residues
MSADRLLTCLAECSTRLDGADTWPVLMKQILALVGEASGATRTWVFQTITLTDQHVIQDHLFEHISEDCWQLQLRKRFRL
FRTALSAPDYHDLVRDRQRGLPSLLVTAEIAPGFLKASLERQKTAAMLSAPILVEGQWWGTIGLDDCQGGADWNATTIKF
LQTIAGLIAGSIHRERQRVRSQQVAVMERTTGGGLWELDVLRGDVWCSETLFHLLGYPPPCARLTIRRLLRHILPEDRAL
VMASARRCMREGLPAFRLDVRAWRHDRTLLWWELIIDVTETRPLDGFPLRMTGIVLEVNDRKTQELELARTAATDPLTGL
ANRRSFEALATRLLENKPGSGTLLVIDIDLFKHINDCFGHAAGDEVLRHLAIVGRSVLRDGDLIGRLGGEEFAVLLQMDG
PEAYAVADRLCQAVREMAVTAPSWEHSSVVVKITISIGIAHPCPFEDPKFALSEALRRADRALYGAKKRGRNQICHDTEG
RQSAISRDNRAKK
>Mature_492_residues
SADRLLTCLAECSTRLDGADTWPVLMKQILALVGEASGATRTWVFQTITLTDQHVIQDHLFEHISEDCWQLQLRKRFRLF
RTALSAPDYHDLVRDRQRGLPSLLVTAEIAPGFLKASLERQKTAAMLSAPILVEGQWWGTIGLDDCQGGADWNATTIKFL
QTIAGLIAGSIHRERQRVRSQQVAVMERTTGGGLWELDVLRGDVWCSETLFHLLGYPPPCARLTIRRLLRHILPEDRALV
MASARRCMREGLPAFRLDVRAWRHDRTLLWWELIIDVTETRPLDGFPLRMTGIVLEVNDRKTQELELARTAATDPLTGLA
NRRSFEALATRLLENKPGSGTLLVIDIDLFKHINDCFGHAAGDEVLRHLAIVGRSVLRDGDLIGRLGGEEFAVLLQMDGP
EAYAVADRLCQAVREMAVTAPSWEHSSVVVKITISIGIAHPCPFEDPKFALSEALRRADRALYGAKKRGRNQICHDTEGR
QSAISRDNRAKK

Specific function: Unknown

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=194, Percent_Identity=36.0824742268041, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1787816, Length=241, Percent_Identity=33.6099585062241, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788381, Length=207, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=36.8098159509202, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1786584, Length=209, Percent_Identity=33.0143540669856, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI145693134, Length=207, Percent_Identity=30.4347826086957, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082007, Length=164, Percent_Identity=37.1951219512195, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788956, Length=174, Percent_Identity=35.0574712643678, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787541, Length=178, Percent_Identity=31.4606741573034, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1787056, Length=214, Percent_Identity=32.7102803738318, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787802, Length=188, Percent_Identity=29.7872340425532, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788085, Length=175, Percent_Identity=31.4285714285714, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87081977, Length=112, Percent_Identity=37.5, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656 [H]

Pfam domain/function: PF00990 GGDEF; PF08448 PAS_4 [H]

EC number: NA

Molecular weight: Translated: 55277; Mature: 55146

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADRLLTCLAECSTRLDGADTWPVLMKQILALVGEASGATRTWVFQTITLTDQHVIQDH
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEECHHHHHHHH
LFEHISEDCWQLQLRKRFRLFRTALSAPDYHDLVRDRQRGLPSLLVTAEIAPGFLKASLE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHH
RQKTAAMLSAPILVEGQWWGTIGLDDCQGGADWNATTIKFLQTIAGLIAGSIHRERQRVR
HHHHHHHHHCCEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SQQVAVMERTTGGGLWELDVLRGDVWCSETLFHLLGYPPPCARLTIRRLLRHILPEDRAL
HHHHHHHEECCCCCEEEEEEHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHH
VMASARRCMREGLPAFRLDVRAWRHDRTLLWWELIIDVTETRPLDGFPLRMTGIVLEVND
HHHHHHHHHHHCCCCEEHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCEEEEEEEEEECC
RKTQELELARTAATDPLTGLANRRSFEALATRLLENKPGSGTLLVIDIDLFKHINDCFGH
CCHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCC
AAGDEVLRHLAIVGRSVLRDGDLIGRLGGEEFAVLLQMDGPEAYAVADRLCQAVREMAVT
CCHHHHHHHHHHHHHHHHCCCCHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHC
APSWEHSSVVVKITISIGIAHPCPFEDPKFALSEALRRADRALYGAKKRGRNQICHDTEG
CCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RQSAISRDNRAKK
HHHHHCCCCCCCC
>Mature Secondary Structure 
SADRLLTCLAECSTRLDGADTWPVLMKQILALVGEASGATRTWVFQTITLTDQHVIQDH
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEECHHHHHHHH
LFEHISEDCWQLQLRKRFRLFRTALSAPDYHDLVRDRQRGLPSLLVTAEIAPGFLKASLE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHH
RQKTAAMLSAPILVEGQWWGTIGLDDCQGGADWNATTIKFLQTIAGLIAGSIHRERQRVR
HHHHHHHHHCCEEEECCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SQQVAVMERTTGGGLWELDVLRGDVWCSETLFHLLGYPPPCARLTIRRLLRHILPEDRAL
HHHHHHHEECCCCCEEEEEEHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHH
VMASARRCMREGLPAFRLDVRAWRHDRTLLWWELIIDVTETRPLDGFPLRMTGIVLEVND
HHHHHHHHHHHCCCCEEHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCEEEEEEEEEECC
RKTQELELARTAATDPLTGLANRRSFEALATRLLENKPGSGTLLVIDIDLFKHINDCFGH
CCHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCC
AAGDEVLRHLAIVGRSVLRDGDLIGRLGGEEFAVLLQMDGPEAYAVADRLCQAVREMAVT
CCHHHHHHHHHHHHHHHHCCCCHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHC
APSWEHSSVVVKITISIGIAHPCPFEDPKFALSEALRRADRALYGAKKRGRNQICHDTEG
CCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RQSAISRDNRAKK
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]