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The map label for this gene is wza [H]

Identifier: 83594447

GI number: 83594447

Start: 3595017

End: 3595646

Strand: Reverse

Name: wza [H]

Synonym: Rru_A3117

Alternate gene names: 83594447

Gene position: 3595646-3595017 (Counterclockwise)

Preceding gene: 83594455

Following gene: 83594446

Centisome position: 82.6

GC content: 63.97

Gene sequence:

>630_bases
ATGATACGCGCGTGCATTGTTCGGAGGCTTTCTCTTCTTTTCGTAGGGGTGGCGTTTCTTGGCGGCCTTGCGGCCTGTTC
GGGTTTGGATGCGCCGCCGGCGACCGGATTCGAGACGGGAGAGCGACCGGTCTATGTGGTCGGCCCCGGGGATTCGCTGG
ATGTCTTCGTCTGGGGGCAGCCCGATCTGTCGCGCTCCATCGTCGTTCGCCCCGACGGCCGCCTGACCCTGCCGCTGGTC
ACCGATCTGGTCGCGGCGGGCAAAACGCCCTCTGAATTATCGGCCGCGATCACCGAGCAGCTGCAGACCTACGTGCAAAA
TCCGGTGGTTTCGGTGATGGTCGTCAATTTCGCCGGCCCCTTCGACAAACAGGTCCGCGTCGTCGGCGAGGCCGCCACGC
CCAAGGCCCTGCAATACCGCGACCGCATGACCACTCTGGACGCGATGATCGAAGTCGGCGGCTTGAAGCAGTTCGCCGCC
GGAAACCGCGCCGTTCTGGTGCGCGGCCAGCCGCCCGAGGCCAAGTCCTACCGCGTCCGTCTGGAAGATCTGATGAACGA
CGGCGACATCACCGCCAATGTGCCGTTGATGCCCGGTGACGTTCTGATCATTCCGCAGAGCTGGTTCTAG

Upstream 100 bases:

>100_bases
ATTGATTTATCCTTTATGAGGATGAGATCCTAATCTATTGCCGTTAACGGATTGCCTTGGTGTGGTTCCATTCTCTGATT
CATTTTGGGGGCGATATAAT

Downstream 100 bases:

>100_bases
GCGATTCGCATGGATGCGGTTGGGGGTCCATGGGAAATGTCGACACACCGGATGAACGCGGTCGGCGGCGGGGCCTCGCG
CTCCGCCGGCGACCGGCAAG

Product: polysaccharide export protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MIRACIVRRLSLLFVGVAFLGGLAACSGLDAPPATGFETGERPVYVVGPGDSLDVFVWGQPDLSRSIVVRPDGRLTLPLV
TDLVAAGKTPSELSAAITEQLQTYVQNPVVSVMVVNFAGPFDKQVRVVGEAATPKALQYRDRMTTLDAMIEVGGLKQFAA
GNRAVLVRGQPPEAKSYRVRLEDLMNDGDITANVPLMPGDVLIIPQSWF

Sequences:

>Translated_209_residues
MIRACIVRRLSLLFVGVAFLGGLAACSGLDAPPATGFETGERPVYVVGPGDSLDVFVWGQPDLSRSIVVRPDGRLTLPLV
TDLVAAGKTPSELSAAITEQLQTYVQNPVVSVMVVNFAGPFDKQVRVVGEAATPKALQYRDRMTTLDAMIEVGGLKQFAA
GNRAVLVRGQPPEAKSYRVRLEDLMNDGDITANVPLMPGDVLIIPQSWF
>Mature_209_residues
MIRACIVRRLSLLFVGVAFLGGLAACSGLDAPPATGFETGERPVYVVGPGDSLDVFVWGQPDLSRSIVVRPDGRLTLPLV
TDLVAAGKTPSELSAAITEQLQTYVQNPVVSVMVVNFAGPFDKQVRVVGEAATPKALQYRDRMTTLDAMIEVGGLKQFAA
GNRAVLVRGQPPEAKSYRVRLEDLMNDGDITANVPLMPGDVLIIPQSWF

Specific function: Probably involved in the export of the extracellular polysaccharide colanic acid from the cell to medium [H]

COG id: COG1596

COG function: function code M; Periplasmic protein involved in polysaccharide export

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BexD/CtrA/VexA family [H]

Homologues:

Organism=Escherichia coli, GI1788376, Length=177, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1787218, Length=175, Percent_Identity=31.4285714285714, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003715
- InterPro:   IPR019554 [H]

Pfam domain/function: PF02563 Poly_export; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 22408; Mature: 22408

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRACIVRRLSLLFVGVAFLGGLAACSGLDAPPATGFETGERPVYVVGPGDSLDVFVWGQ
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECC
PDLSRSIVVRPDGRLTLPLVTDLVAAGKTPSELSAAITEQLQTYVQNPVVSVMVVNFAGP
CCCCCEEEECCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCC
FDKQVRVVGEAATPKALQYRDRMTTLDAMIEVGGLKQFAAGNRAVLVRGQPPEAKSYRVR
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEECCCCCCCHHEEE
LEDLMNDGDITANVPLMPGDVLIIPQSWF
HHHHCCCCCCEEECCCCCCCEEEECCCCC
>Mature Secondary Structure
MIRACIVRRLSLLFVGVAFLGGLAACSGLDAPPATGFETGERPVYVVGPGDSLDVFVWGQ
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECC
PDLSRSIVVRPDGRLTLPLVTDLVAAGKTPSELSAAITEQLQTYVQNPVVSVMVVNFAGP
CCCCCEEEECCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCC
FDKQVRVVGEAATPKALQYRDRMTTLDAMIEVGGLKQFAAGNRAVLVRGQPPEAKSYRVR
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEEECCCCCCCHHEEE
LEDLMNDGDITANVPLMPGDVLIIPQSWF
HHHHCCCCCCEEECCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]