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The map label for this gene is rnhA

Identifier: 83594384

GI number: 83594384

Start: 3517282

End: 3517782

Strand: Direct

Name: rnhA

Synonym: Rru_A3054

Alternate gene names: 83594384

Gene position: 3517282-3517782 (Clockwise)

Preceding gene: 83594383

Following gene: 83594389

Centisome position: 80.8

GC content: 65.87

Gene sequence:

>501_bases
ATGAGCGCGGCGGCCGGCGACGAGATCAAGCGCGTGCGCGTCGATATGTTCACCGATGGCGCCTGTTCGGGCAATCCGGG
CCCGGGCGGCTGGGGCACCATCTTGCGCTGGGGCGACACCGAAAAGGAGTTGTGGGGCGGCGAAACGCCGACCACCAACA
ACCGCATGGAATTGATGGCGGTGATTCGCGGCCTGGAGGCCCTGCGCCGGCCGGTCACGGTGACCATCCACACCGACAGC
CGCTATGTCCACGACGGTATCACCGGCTGGATCCACGGCTGGAAGCGCAACGGCTGGAAGACGGCGGCCAAGAAGCCGGT
GAAGAACGAAGACCTGTGGCGCCGCCTGGACGCGGCTTTGGGCACCCATGACATCAGTTGGCAATGGGTGCGCGGCCATT
CGGGACATGTGGAAAACGAACGCGCCGACGAATTGGCCCGTCGCGGCACCAGCGAAGCCCGCCAGGGCAAGGTCGACGGC
CAGTCTTCGACCATCCTTTAG

Upstream 100 bases:

>100_bases
CGCCGGGCGCCATGGTCCGGCCAAAGGATCCGATGGAATATTATCACAAGCTGGCCTTCCACCGCGCCGTCTCCGGCCCG
GGCGCCTATGGATTGAGCCG

Downstream 100 bases:

>100_bases
CCCGGCTTCTTTAAGCCCGCCGGCTCGCCGTCCAGCACAGCAACGACCCCGCCACCACCAACCCCGTGCCCATCCAAAAC
GAGGGGGTGGGGGTGGTGTC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MSAAAGDEIKRVRVDMFTDGACSGNPGPGGWGTILRWGDTEKELWGGETPTTNNRMELMAVIRGLEALRRPVTVTIHTDS
RYVHDGITGWIHGWKRNGWKTAAKKPVKNEDLWRRLDAALGTHDISWQWVRGHSGHVENERADELARRGTSEARQGKVDG
QSSTIL

Sequences:

>Translated_166_residues
MSAAAGDEIKRVRVDMFTDGACSGNPGPGGWGTILRWGDTEKELWGGETPTTNNRMELMAVIRGLEALRRPVTVTIHTDS
RYVHDGITGWIHGWKRNGWKTAAKKPVKNEDLWRRLDAALGTHDISWQWVRGHSGHVENERADELARRGTSEARQGKVDG
QSSTIL
>Mature_165_residues
SAAAGDEIKRVRVDMFTDGACSGNPGPGGWGTILRWGDTEKELWGGETPTTNNRMELMAVIRGLEALRRPVTVTIHTDSR
YVHDGITGWIHGWKRNGWKTAAKKPVKNEDLWRRLDAALGTHDISWQWVRGHSGHVENERADELARRGTSEARQGKVDGQ
SSTIL

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=148, Percent_Identity=37.1621621621622, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI1786408, Length=135, Percent_Identity=65.9259259259259, Blast_Score=189, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=41.1347517730496, Blast_Score=96, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17505482, Length=132, Percent_Identity=37.8787878787879, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45552509, Length=149, Percent_Identity=34.8993288590604, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17137476, Length=149, Percent_Identity=34.8993288590604, Blast_Score=80, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_RHORT (Q2RPU6)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428136.1
- HSSP:   P0A7Y4
- ProteinModelPortal:   Q2RPU6
- SMR:   Q2RPU6
- STRING:   Q2RPU6
- GeneID:   3836500
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3054
- NMPDR:   fig|1085.1.peg.695
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q2RPU6
- BioCyc:   RRUB269796:RRU_A3054-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 18483; Mature: 18352

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAAGDEIKRVRVDMFTDGACSGNPGPGGWGTILRWGDTEKELWGGETPTTNNRMELMA
CCCCCCCCHHEEEEEEEECCCCCCCCCCCCCCCEEECCCCHHHHCCCCCCCCCCHHHHHH
VIRGLEALRRPVTVTIHTDSRYVHDGITGWIHGWKRNGWKTAAKKPVKNEDLWRRLDAAL
HHHHHHHHCCCEEEEEECCCEEECCCHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHH
GTHDISWQWVRGHSGHVENERADELARRGTSEARQGKVDGQSSTIL
CCCCCCEEEECCCCCCCCCHHHHHHHHCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SAAAGDEIKRVRVDMFTDGACSGNPGPGGWGTILRWGDTEKELWGGETPTTNNRMELMA
CCCCCCCHHEEEEEEEECCCCCCCCCCCCCCCEEECCCCHHHHCCCCCCCCCCHHHHHH
VIRGLEALRRPVTVTIHTDSRYVHDGITGWIHGWKRNGWKTAAKKPVKNEDLWRRLDAAL
HHHHHHHHCCCEEEEEECCCEEECCCHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHH
GTHDISWQWVRGHSGHVENERADELARRGTSEARQGKVDGQSSTIL
CCCCCCEEEECCCCCCCCCHHHHHHHHCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA