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The map label for this gene is alaXM [H]

Identifier: 83594299

GI number: 83594299

Start: 3419761

End: 3420405

Strand: Direct

Name: alaXM [H]

Synonym: Rru_A2969

Alternate gene names: 83594299

Gene position: 3419761-3420405 (Clockwise)

Preceding gene: 83594298

Following gene: 83594300

Centisome position: 78.56

GC content: 66.67

Gene sequence:

>645_bases
ATGACCCATAAACTGTTCTGGGACGACCCCTATCGCCAAACCCTCGACACCCGCGTCACCCGGGTGGCGGGGCCCGAGGT
CATGGTCGGCGAAACCATCCTCTATGCCTTTTCAGGCGGCCAGGAAAGCGATCACGGCACGATCGGCGGCCGCCCCGTGT
TGGGCGCGCGCAAGGATGGCCTGACCATCCTCTACGACCTGGGCCCCGATCACGGCCTAAGCCCGGGCGACCCGGTCGGT
CTGGTTCTCGACTGGTCGCGCCGCTACCGGCTGATGCGCCTGCATTTCGCCGCCGAGATCATTCTGGAACTGGTGATGCG
CGATTTCGGCCCCCTGGAAAAGACCGGCGCCCATATCGCCCCCGACAAGGCGCGGATCGACTTCACCTGGGAGGGCTCGA
TCGCCCCGATCTTTCCCAGCCTGCTCGAATCGAGCGGGGCGGTGATCGCCCGCGACCTGCCGATCATCACCGCCTTTTCC
GACGAGGCCAGCCAGCGCCGCTATTGGGAAATCGAGGGCTTCGCCCGCGTGCCCTGCGGCGGCACCCACCCCCGGCGAAC
CGGGGAAATCGGCCCGCTCGCCCTCAAACGCAAGAACATCGGCAAGGGCCGCGAGCGCATCGAAGTGCTGCTGGCTGGGG
ATTAG

Upstream 100 bases:

>100_bases
GGTGACCGACGGCCGCACCCCGACCATCGAACGGCGCGAGAAGGGGTTCTTGGAAGGGATTTTCTAAACCCGGCTCCCCC
TCCGGATCCAAGGGACCGCG

Downstream 100 bases:

>100_bases
CCGCCGGCCAAAAGGAGACGCTTGCGCCATGCGTCACGTTTCGATCATCGGCATCGGCCCGGGCGATCCCGACTGCCTGA
CCTTGCGGGCGGCGCGACGG

Product: alanyl-tRNA synthetase related protein

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTHKLFWDDPYRQTLDTRVTRVAGPEVMVGETILYAFSGGQESDHGTIGGRPVLGARKDGLTILYDLGPDHGLSPGDPVG
LVLDWSRRYRLMRLHFAAEIILELVMRDFGPLEKTGAHIAPDKARIDFTWEGSIAPIFPSLLESSGAVIARDLPIITAFS
DEASQRRYWEIEGFARVPCGGTHPRRTGEIGPLALKRKNIGKGRERIEVLLAGD

Sequences:

>Translated_214_residues
MTHKLFWDDPYRQTLDTRVTRVAGPEVMVGETILYAFSGGQESDHGTIGGRPVLGARKDGLTILYDLGPDHGLSPGDPVG
LVLDWSRRYRLMRLHFAAEIILELVMRDFGPLEKTGAHIAPDKARIDFTWEGSIAPIFPSLLESSGAVIARDLPIITAFS
DEASQRRYWEIEGFARVPCGGTHPRRTGEIGPLALKRKNIGKGRERIEVLLAGD
>Mature_213_residues
THKLFWDDPYRQTLDTRVTRVAGPEVMVGETILYAFSGGQESDHGTIGGRPVLGARKDGLTILYDLGPDHGLSPGDPVGL
VLDWSRRYRLMRLHFAAEIILELVMRDFGPLEKTGAHIAPDKARIDFTWEGSIAPIFPSLLESSGAVIARDLPIITAFSD
EASQRRYWEIEGFARVPCGGTHPRRTGEIGPLALKRKNIGKGRERIEVLLAGD

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged Gly-tRNA(Ala) and Ser-tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 23658; Mature: 23527

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHKLFWDDPYRQTLDTRVTRVAGPEVMVGETILYAFSGGQESDHGTIGGRPVLGARKDG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEECCEEEEEEECCCCCCCCCCCCEECCCCCCCC
LTILYDLGPDHGLSPGDPVGLVLDWSRRYRLMRLHFAAEIILELVMRDFGPLEKTGAHIA
CEEEEECCCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCCHHHCCCEEC
PDKARIDFTWEGSIAPIFPSLLESSGAVIARDLPIITAFSDEASQRRYWEIEGFARVPCG
CCCEEEEEEECCCCHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCEEEEECCEEECCCC
GTHPRRTGEIGPLALKRKNIGKGRERIEVLLAGD
CCCCCCCCCCCCEEEECCCCCCCCEEEEEEEECC
>Mature Secondary Structure 
THKLFWDDPYRQTLDTRVTRVAGPEVMVGETILYAFSGGQESDHGTIGGRPVLGARKDG
CCCCCCCCHHHHHHHHHHHHHCCCCEEECCEEEEEEECCCCCCCCCCCCEECCCCCCCC
LTILYDLGPDHGLSPGDPVGLVLDWSRRYRLMRLHFAAEIILELVMRDFGPLEKTGAHIA
CEEEEECCCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCCHHHCCCEEC
PDKARIDFTWEGSIAPIFPSLLESSGAVIARDLPIITAFSDEASQRRYWEIEGFARVPCG
CCCEEEEEEECCCCHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCEEEEECCEEECCCC
GTHPRRTGEIGPLALKRKNIGKGRERIEVLLAGD
CCCCCCCCCCCCEEEECCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]