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The map label for this gene is yhcK [H]

Identifier: 83593899

GI number: 83593899

Start: 2986624

End: 2987817

Strand: Reverse

Name: yhcK [H]

Synonym: Rru_A2567

Alternate gene names: 83593899

Gene position: 2987817-2986624 (Counterclockwise)

Preceding gene: 83593900

Following gene: 83593898

Centisome position: 68.64

GC content: 65.16

Gene sequence:

>1194_bases
GTGATCGATACGACGACGATGATGCTGGTGCTTGGACTGGGCAATCTGCTGTTTGGTCTGACCATGGCCGCCTACCGCCA
GCCCGCCGCCGATCATCGGGTGATGCGGCTGTGGATCGCCGCCCAGGTTTTGAAGGGGATCGGCACCCTTCTTGTCACGC
TGCGCGGCGAGGTCGGACTGAGTTTGAGCATCTTCGGCGGCAACTCGCTGATCTTCATCGGCTATGGCCTGGAGGTCGCC
GCCTATCGCGCCTATGCCAAGCGCCCCAATGCCGATGCCTTGCTGCCCTTGCTGATCGTCGGCTATCTGGTGCTGTTCAA
TGGCGCGGCGGGGATCACCCCGCCCGATGCGCCCCGCAATCATCTGTTTTTCCTGTCCTCGGCGATGCTCGCCAGTCTGG
GCTTTCTAAACTGCTGGTTCTTGCTGCGGGCCAAGCGCCCGCCCTCGCCGGTCAAGACCTTGTTGATCTCCAGCAATGCC
CTTCTGGGCTTGGCGGCCGTGGGGCGGGCGGTGGGCAGCCTGCTCGCCGGGCCGCTGTCGCCCTTCGCCAATTCGCCGAC
CCATCTGGTGGTCTATGGTTTGGCCTATGCCTTCATGATCACCAACGGCTTTGGCTTTCTTCTGTTGGTCAAGGAGGATA
CCGACGCCGCCTTGCGACGGCTGGCCGATACGGACCAGATGACCGGTCTGGCCAACCGCCGGCACTTCCTGACGGCAAGC
CAGGAGGCCTGGAAACTGGCCGGGCGGCTGGGGCGGCCGACGGCGCTGATGATGTTCGATGTCGATCATTTCAAGCAGGT
CAACGATAGTCAGGGTCATGCCGCCGGCGATGCGGCGCTGGTCGATCTGGCGCGGAACGTTCGCGAGGTCCTGCGCGATA
TCGATATTTTCGGGCGCATGGGCGGCGAGGAATTCGCCATCACCCTGCCGGGCTGCGATCTGGTGGCGGCGGCCGTGATC
GCCGAGCGCGTCCGCCGCGCCGTGGCCGAGAGCCGGGGCGAGGGGCCAGCGATCACCATCAGCCTGGGACTGACGATTCT
GGTCGCCGGAGAGCCTTTTGACACGGCGCTGAAGCGCGCCGACCACGGCCTGTACCAAGCCAAGGCGGCTGGCCGCAACC
GGGTATGCTGCGTTCCCGCTCCCCAGGGCTTGGCCAGCGTGGAGGCCGAAGCACCGATTTCCCTGGCGGGGTAG

Upstream 100 bases:

>100_bases
ACGAGAAGGCGCGCTGCGGCTGCGGCGAGAGCTTTTCCGTTTGACGGGCGCGGGGGCGGCCGTTTGAAGAAGGATAAAGC
GCTAGCATAAGGGGCCCGGC

Downstream 100 bases:

>100_bases
AGGTTGGCCATCAAAAACCCAAAAGTGTTTTTCTTGGGAGAATTGAAGCCCCCCCCGAAACCTGGGACGGAGAAGGAAAA
CGTAAGACCCCAAAAAATCA

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MIDTTTMMLVLGLGNLLFGLTMAAYRQPAADHRVMRLWIAAQVLKGIGTLLVTLRGEVGLSLSIFGGNSLIFIGYGLEVA
AYRAYAKRPNADALLPLLIVGYLVLFNGAAGITPPDAPRNHLFFLSSAMLASLGFLNCWFLLRAKRPPSPVKTLLISSNA
LLGLAAVGRAVGSLLAGPLSPFANSPTHLVVYGLAYAFMITNGFGFLLLVKEDTDAALRRLADTDQMTGLANRRHFLTAS
QEAWKLAGRLGRPTALMMFDVDHFKQVNDSQGHAAGDAALVDLARNVREVLRDIDIFGRMGGEEFAITLPGCDLVAAAVI
AERVRRAVAESRGEGPAITISLGLTILVAGEPFDTALKRADHGLYQAKAAGRNRVCCVPAPQGLASVEAEAPISLAG

Sequences:

>Translated_397_residues
MIDTTTMMLVLGLGNLLFGLTMAAYRQPAADHRVMRLWIAAQVLKGIGTLLVTLRGEVGLSLSIFGGNSLIFIGYGLEVA
AYRAYAKRPNADALLPLLIVGYLVLFNGAAGITPPDAPRNHLFFLSSAMLASLGFLNCWFLLRAKRPPSPVKTLLISSNA
LLGLAAVGRAVGSLLAGPLSPFANSPTHLVVYGLAYAFMITNGFGFLLLVKEDTDAALRRLADTDQMTGLANRRHFLTAS
QEAWKLAGRLGRPTALMMFDVDHFKQVNDSQGHAAGDAALVDLARNVREVLRDIDIFGRMGGEEFAITLPGCDLVAAAVI
AERVRRAVAESRGEGPAITISLGLTILVAGEPFDTALKRADHGLYQAKAAGRNRVCCVPAPQGLASVEAEAPISLAG
>Mature_397_residues
MIDTTTMMLVLGLGNLLFGLTMAAYRQPAADHRVMRLWIAAQVLKGIGTLLVTLRGEVGLSLSIFGGNSLIFIGYGLEVA
AYRAYAKRPNADALLPLLIVGYLVLFNGAAGITPPDAPRNHLFFLSSAMLASLGFLNCWFLLRAKRPPSPVKTLLISSNA
LLGLAAVGRAVGSLLAGPLSPFANSPTHLVVYGLAYAFMITNGFGFLLLVKEDTDAALRRLADTDQMTGLANRRHFLTAS
QEAWKLAGRLGRPTALMMFDVDHFKQVNDSQGHAAGDAALVDLARNVREVLRDIDIFGRMGGEEFAITLPGCDLVAAAVI
AERVRRAVAESRGEGPAITISLGLTILVAGEPFDTALKRADHGLYQAKAAGRNRVCCVPAPQGLASVEAEAPISLAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=162, Percent_Identity=38.2716049382716, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1786584, Length=225, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=32.9268292682927, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=37.7245508982036, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788381, Length=204, Percent_Identity=34.3137254901961, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI145693134, Length=180, Percent_Identity=31.6666666666667, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787802, Length=169, Percent_Identity=30.7692307692308, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=31.547619047619, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787816, Length=294, Percent_Identity=28.2312925170068, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081974, Length=151, Percent_Identity=29.1390728476821, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=164, Percent_Identity=29.2682926829268, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788956, Length=175, Percent_Identity=30.2857142857143, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620 [H]

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 42161; Mature: 42161

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDTTTMMLVLGLGNLLFGLTMAAYRQPAADHRVMRLWIAAQVLKGIGTLLVTLRGEVGL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCE
SLSIFGGNSLIFIGYGLEVAAYRAYAKRPNADALLPLLIVGYLVLFNGAAGITPPDAPRN
EEEEECCCEEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
HLFFLSSAMLASLGFLNCWFLLRAKRPPSPVKTLLISSNALLGLAAVGRAVGSLLAGPLS
CEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHCCCC
PFANSPTHLVVYGLAYAFMITNGFGFLLLVKEDTDAALRRLADTDQMTGLANRRHFLTAS
CCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCHHHHHHHCCCCEEEECC
QEAWKLAGRLGRPTALMMFDVDHFKQVNDSQGHAAGDAALVDLARNVREVLRDIDIFGRM
HHHHHHHHCCCCCEEEEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GGEEFAITLPGCDLVAAAVIAERVRRAVAESRGEGPAITISLGLTILVAGEPFDTALKRA
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECEEEEEECCCHHHHHHHH
DHGLYQAKAAGRNRVCCVPAPQGLASVEAEAPISLAG
HCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIDTTTMMLVLGLGNLLFGLTMAAYRQPAADHRVMRLWIAAQVLKGIGTLLVTLRGEVGL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCE
SLSIFGGNSLIFIGYGLEVAAYRAYAKRPNADALLPLLIVGYLVLFNGAAGITPPDAPRN
EEEEECCCEEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
HLFFLSSAMLASLGFLNCWFLLRAKRPPSPVKTLLISSNALLGLAAVGRAVGSLLAGPLS
CEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHCCCC
PFANSPTHLVVYGLAYAFMITNGFGFLLLVKEDTDAALRRLADTDQMTGLANRRHFLTAS
CCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCHHHHHHHCCCCEEEECC
QEAWKLAGRLGRPTALMMFDVDHFKQVNDSQGHAAGDAALVDLARNVREVLRDIDIFGRM
HHHHHHHHCCCCCEEEEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GGEEFAITLPGCDLVAAAVIAERVRRAVAESRGEGPAITISLGLTILVAGEPFDTALKRA
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECEEEEEECCCHHHHHHHH
DHGLYQAKAAGRNRVCCVPAPQGLASVEAEAPISLAG
HCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]