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The map label for this gene is ycjG [H]

Identifier: 83593891

GI number: 83593891

Start: 2972623

End: 2973624

Strand: Reverse

Name: ycjG [H]

Synonym: Rru_A2556

Alternate gene names: 83593891

Gene position: 2973624-2972623 (Counterclockwise)

Preceding gene: 83593892

Following gene: 83593890

Centisome position: 68.31

GC content: 69.76

Gene sequence:

>1002_bases
ATGACAAAGCCCCGGCTGATCATCCGCGACGAGGTTTGGCCCATCGCCGGCTCCTTCGTGATTTCCCGCGGTGCCAAGAC
CGCCGCCCATGTGGTTCTCGCCGAGATCCACGAGACCCAGGCGGACGGACGGACGCTGATCGGCCGGGGCGAATGCGTGC
CCTATGCCCGCTATGACGAAACAGTCGAGGGAGTGAAGGCCGATCTGGCCGCCATGACCGAAGCCGTGGCCGAGGGGCTG
ACTCCGACCGGCCTGCTGTCGGCGATGGCCGCCGGGGCGGCGCGCAATGCCCTTGATTGCGCCCTGCTCGATCTGGCGGC
CAAGCGCAGCGGCCGGCCGGTCTGGTCCACCCTGGGGCTGGCCGAGCCCCAGCCGATGGTGACGGCCGAGACCATCAGCA
TCGGCACTCCGGCCGAGATGGAGGCCAAGGCGAAGACCCTGGCCGATCGCCCCTTGCTCAAGATCAAGGTCGGCGCCGAT
GATCCGCTGGGGCGGGTCGCCGCCGTGCGGCGCGGCGCGCCGGACTCGCGGCTGATCGTCGATGCCAACGAGGCTTGGAC
TCCGCGCGATCTGACCGATCTGTTGACGGCGATGGCCGGGCTCAAGGTCGATCTGATCGAACAGCCGCTGCCCGCCGGGG
CCGATGACGCCCTGGCCGATATTCTGAGCCCGGTTCCGCTTTGCGCCGACGAGTCGGCCCATGTCGCCGGCGATGTGCCG
CGCTTGGCCCGGCTTTACAGCCATATCAACATCAAGCTCGATAAGACCGGCGGGCTGACCGGGGCCATCGAACTGGCCGA
TCGCGCCGAGACCGCCGGCCTGGGCCTGATGGTCGGCTGCATGCTGGCCACCTCGCTGGCGATGGCGCCCGCCCTGTTGC
TGGGCGGCCGCGCCGCCTTTGTCGATCTGGATGGCCCGGTGCTGCTGGCCCGCGACCGCGAGCCGGGGCTGCGCTTCACC
GCCGGCGTCATCCATCCCCCTGGCAGCGAGCTCTGGGGATAG

Upstream 100 bases:

>100_bases
CTGGCCGAGACTTCGGCGCGCTTCGCTCTGCCCTGCGTCGATCCGGTGCGCGGGGGTGTCGCCGCCCTGGTCGATCGTCT
GGCCGAGAAGGGCTGATCCC

Downstream 100 bases:

>100_bases
GGACGGTCCTCCCCCGGGGGGGAAGGGGGACTTCGTGGAGGATTTATTTTACTTCCTATAAACTACCCCCTATCCTTCCA
AAAAATGGTTCTTTAGGGAG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTKPRLIIRDEVWPIAGSFVISRGAKTAAHVVLAEIHETQADGRTLIGRGECVPYARYDETVEGVKADLAAMTEAVAEGL
TPTGLLSAMAAGAARNALDCALLDLAAKRSGRPVWSTLGLAEPQPMVTAETISIGTPAEMEAKAKTLADRPLLKIKVGAD
DPLGRVAAVRRGAPDSRLIVDANEAWTPRDLTDLLTAMAGLKVDLIEQPLPAGADDALADILSPVPLCADESAHVAGDVP
RLARLYSHINIKLDKTGGLTGAIELADRAETAGLGLMVGCMLATSLAMAPALLLGGRAAFVDLDGPVLLARDREPGLRFT
AGVIHPPGSELWG

Sequences:

>Translated_333_residues
MTKPRLIIRDEVWPIAGSFVISRGAKTAAHVVLAEIHETQADGRTLIGRGECVPYARYDETVEGVKADLAAMTEAVAEGL
TPTGLLSAMAAGAARNALDCALLDLAAKRSGRPVWSTLGLAEPQPMVTAETISIGTPAEMEAKAKTLADRPLLKIKVGAD
DPLGRVAAVRRGAPDSRLIVDANEAWTPRDLTDLLTAMAGLKVDLIEQPLPAGADDALADILSPVPLCADESAHVAGDVP
RLARLYSHINIKLDKTGGLTGAIELADRAETAGLGLMVGCMLATSLAMAPALLLGGRAAFVDLDGPVLLARDREPGLRFT
AGVIHPPGSELWG
>Mature_332_residues
TKPRLIIRDEVWPIAGSFVISRGAKTAAHVVLAEIHETQADGRTLIGRGECVPYARYDETVEGVKADLAAMTEAVAEGLT
PTGLLSAMAAGAARNALDCALLDLAAKRSGRPVWSTLGLAEPQPMVTAETISIGTPAEMEAKAKTLADRPLLKIKVGADD
PLGRVAAVRRGAPDSRLIVDANEAWTPRDLTDLLTAMAGLKVDLIEQPLPAGADDALADILSPVPLCADESAHVAGDVPR
LARLYSHINIKLDKTGGLTGAIELADRAETAGLGLMVGCMLATSLAMAPALLLGGRAAFVDLDGPVLLARDREPGLRFTA
GVIHPPGSELWG

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=320, Percent_Identity=44.0625, Blast_Score=248, Evalue=5e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 34714; Mature: 34583

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPRLIIRDEVWPIAGSFVISRGAKTAAHVVLAEIHETQADGRTLIGRGECVPYARYDE
CCCCCEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHH
TVEGVKADLAAMTEAVAEGLTPTGLLSAMAAGAARNALDCALLDLAAKRSGRPVWSTLGL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHCCC
AEPQPMVTAETISIGTPAEMEAKAKTLADRPLLKIKVGADDPLGRVAAVRRGAPDSRLIV
CCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCEEEE
DANEAWTPRDLTDLLTAMAGLKVDLIEQPLPAGADDALADILSPVPLCADESAHVAGDVP
ECCCCCCCHHHHHHHHHHHCCEEEHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHH
RLARLYSHINIKLDKTGGLTGAIELADRAETAGLGLMVGCMLATSLAMAPALLLGGRAAF
HHHHHHHHEEEEEECCCCCEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
VDLDGPVLLARDREPGLRFTAGVIHPPGSELWG
EECCCCEEEEECCCCCCEEEEEEECCCCHHCCC
>Mature Secondary Structure 
TKPRLIIRDEVWPIAGSFVISRGAKTAAHVVLAEIHETQADGRTLIGRGECVPYARYDE
CCCCEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHH
TVEGVKADLAAMTEAVAEGLTPTGLLSAMAAGAARNALDCALLDLAAKRSGRPVWSTLGL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHCCC
AEPQPMVTAETISIGTPAEMEAKAKTLADRPLLKIKVGADDPLGRVAAVRRGAPDSRLIV
CCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCEEEE
DANEAWTPRDLTDLLTAMAGLKVDLIEQPLPAGADDALADILSPVPLCADESAHVAGDVP
ECCCCCCCHHHHHHHHHHHCCEEEHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHH
RLARLYSHINIKLDKTGGLTGAIELADRAETAGLGLMVGCMLATSLAMAPALLLGGRAAF
HHHHHHHHEEEEEECCCCCEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
VDLDGPVLLARDREPGLRFTAGVIHPPGSELWG
EECCCCEEEEECCCCCCEEEEEEECCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]