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The map label for this gene is amiC [H]

Identifier: 83593483

GI number: 83593483

Start: 2493887

End: 2495455

Strand: Reverse

Name: amiC [H]

Synonym: Rru_A2148

Alternate gene names: 83593483

Gene position: 2495455-2493887 (Counterclockwise)

Preceding gene: 83593486

Following gene: 83593482

Centisome position: 57.33

GC content: 68.51

Gene sequence:

>1569_bases
ATGGAACGATACGTGGGCCGAGGGACGACACCGCCGATGGCGCGCCGCAAAGCAACCGGAGTGAGCCGACGCAGCCTGCT
GGCGGGAACCCCGTTCGTCGCCGCCGCCTTGCTGGCGCCCCGGCCGGCGCTGGCCGCGACCCGGGCGACCGGCCTGCGGT
TGGGCGACCATGGCGGCTCGACGCGCTTCGTGCTCGAGCTTTCGGGGCCGGTGACGTGGCAACTCGTCTCCCTCTCGGCG
CCCTATCGCCTGATCATCGATATGCCCGAGGTCGAGTTCGCCCTGGGGCTGGTGCCCCGCGACACCGGTCTGGTCGCCGG
CGCCCGCTATGGCGTGGTGCGGCCCGGCTTCTCGCGCATGGTCATCGACCTCACCGGCCCGGCGATGGTCGACAAGGACT
TCCTGCTGGAACCGCGCGAGGGGTTCGGCTGGCGGCTGGTCATGGATCTGGTGCCGACCACGCCCGAGATCTTCATGGCC
GGGGTTGGTCAGCCGGCCAAGCCCGTCGTCCCCGCCACCGTCACCCCCGAGCCCTCGACCCGCGCCGTGGCCTTCGCCGC
CGCCGCCGCTCCCGCCGCCGCCGCGAGCGGGACGACGGCGCCCACACCCATCGGTCCGGGCGGCCCGGTCAATCTGCGGC
CGGCCGCCGTGGGCAAGGGGCCGGTCACGGTCAATCTCGCCTCTTTGCCCTCGGCCCAGGCGCCGATCGCCGATCCGTCG
ATTCCCGGCTCGTTTGGCGGTCCAGGCGGGGCGGCGGCGGGCGAGGGGATGCAGGTGATGGCGCGCGAGGGGCCGGCGAT
CGTCCGGCTGGCCGACGGCCGGCGCGTGCCCATTCCCTTGGAAAAGCCCCAGCGCTCGGTCGATCCGGTGCGCAAGCCGG
TGATCGTGCTTGATCCCGGCCATGGCGGCAAGGACCCCGGGGCCATCGGCGCTTCGGGAACCTATGAAAAGATCGTCACC
CTCGAGATGGCCCGCCAGCTCAAACGCGCCCTGGAGGCCACCGGCCGCTACAAGGTGGTTCTGACCCGCGAGAGCGACAC
TTCGGTTCGCCTGCGCGAACGCATCGCCTTTGGCCGCCATGCCGGGGCCGATCTGTTCGTGTCGATCCATGCCGATGCCA
TGGCCAATCCCCAGGTGCGCGGGCTGTCGGTCTATACCTTGTCGGAAACCGCTTCCGACGACGAGGCCGCCCAGCTGGCC
GATCGTGAAAACAAGGTCGATATCCTGCTGGGCATGGATCTGTCCCAGGAATCGCCCGATGTCGCGACGATTTTGATCGA
TCTCGCCCAGCGCGAGACCAAGAACAAATCGGTTCATTTCGCCAATACCCTGGTCTCGGCCTTGCCCGGTGATGTGTTGA
AGCTGGAAAAGACCCGCCGGTATGCCGGTTTCGCGGTTCTGAAGGCGCCCGACGTTCCCTCGGTCCTTGTGGAAATGGGC
TTCCTTTCCAATGTGTCCGACGAAAAACTGTTGCGCACCGCCCCCTATCGGGCGAAGCTCGCCGCCGCTCTTGTCCGCAG
CGTCGATGATTTCTTCGCCCCCCAGCAGCAACGCGCCTGGAAACCTTAA

Upstream 100 bases:

>100_bases
TAATTATCCATGGTAAATCATAGGGTAATCGCGGCTTCTTAGATCGAAATCTTGTCAATTGGGCCGCTGTCCCCTATACC
TGTGGTGGGACTTTCAAACC

Downstream 100 bases:

>100_bases
CGGTTTTCGCGCGATCGGCCCTGTCGATCGCCCGCCGGGATGAACGGGCGCCTTGACCGCGCCACGATCGTGGTGCAAAG
ACCGCAGGCAAGAAAACGCC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MERYVGRGTTPPMARRKATGVSRRSLLAGTPFVAAALLAPRPALAATRATGLRLGDHGGSTRFVLELSGPVTWQLVSLSA
PYRLIIDMPEVEFALGLVPRDTGLVAGARYGVVRPGFSRMVIDLTGPAMVDKDFLLEPREGFGWRLVMDLVPTTPEIFMA
GVGQPAKPVVPATVTPEPSTRAVAFAAAAAPAAAASGTTAPTPIGPGGPVNLRPAAVGKGPVTVNLASLPSAQAPIADPS
IPGSFGGPGGAAAGEGMQVMAREGPAIVRLADGRRVPIPLEKPQRSVDPVRKPVIVLDPGHGGKDPGAIGASGTYEKIVT
LEMARQLKRALEATGRYKVVLTRESDTSVRLRERIAFGRHAGADLFVSIHADAMANPQVRGLSVYTLSETASDDEAAQLA
DRENKVDILLGMDLSQESPDVATILIDLAQRETKNKSVHFANTLVSALPGDVLKLEKTRRYAGFAVLKAPDVPSVLVEMG
FLSNVSDEKLLRTAPYRAKLAAALVRSVDDFFAPQQQRAWKP

Sequences:

>Translated_522_residues
MERYVGRGTTPPMARRKATGVSRRSLLAGTPFVAAALLAPRPALAATRATGLRLGDHGGSTRFVLELSGPVTWQLVSLSA
PYRLIIDMPEVEFALGLVPRDTGLVAGARYGVVRPGFSRMVIDLTGPAMVDKDFLLEPREGFGWRLVMDLVPTTPEIFMA
GVGQPAKPVVPATVTPEPSTRAVAFAAAAAPAAAASGTTAPTPIGPGGPVNLRPAAVGKGPVTVNLASLPSAQAPIADPS
IPGSFGGPGGAAAGEGMQVMAREGPAIVRLADGRRVPIPLEKPQRSVDPVRKPVIVLDPGHGGKDPGAIGASGTYEKIVT
LEMARQLKRALEATGRYKVVLTRESDTSVRLRERIAFGRHAGADLFVSIHADAMANPQVRGLSVYTLSETASDDEAAQLA
DRENKVDILLGMDLSQESPDVATILIDLAQRETKNKSVHFANTLVSALPGDVLKLEKTRRYAGFAVLKAPDVPSVLVEMG
FLSNVSDEKLLRTAPYRAKLAAALVRSVDDFFAPQQQRAWKP
>Mature_522_residues
MERYVGRGTTPPMARRKATGVSRRSLLAGTPFVAAALLAPRPALAATRATGLRLGDHGGSTRFVLELSGPVTWQLVSLSA
PYRLIIDMPEVEFALGLVPRDTGLVAGARYGVVRPGFSRMVIDLTGPAMVDKDFLLEPREGFGWRLVMDLVPTTPEIFMA
GVGQPAKPVVPATVTPEPSTRAVAFAAAAAPAAAASGTTAPTPIGPGGPVNLRPAAVGKGPVTVNLASLPSAQAPIADPS
IPGSFGGPGGAAAGEGMQVMAREGPAIVRLADGRRVPIPLEKPQRSVDPVRKPVIVLDPGHGGKDPGAIGASGTYEKIVT
LEMARQLKRALEATGRYKVVLTRESDTSVRLRERIAFGRHAGADLFVSIHADAMANPQVRGLSVYTLSETASDDEAAQLA
DRENKVDILLGMDLSQESPDVATILIDLAQRETKNKSVHFANTLVSALPGDVLKLEKTRRYAGFAVLKAPDVPSVLVEMG
FLSNVSDEKLLRTAPYRAKLAAALVRSVDDFFAPQQQRAWKP

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=222, Percent_Identity=41.8918918918919, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788776, Length=223, Percent_Identity=40.8071748878924, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790611, Length=227, Percent_Identity=37.4449339207048, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 55026; Mature: 55026

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERYVGRGTTPPMARRKATGVSRRSLLAGTPFVAAALLAPRPALAATRATGLRLGDHGGS
CCCCCCCCCCCCHHHHHHCCCCHHHHHCCCHHHHHHHHCCCCHHHHHHHCCEEEECCCCC
TRFVLELSGPVTWQLVSLSAPYRLIIDMPEVEFALGLVPRDTGLVAGARYGVVRPGFSRM
EEEEEEECCCCEEEEEEECCCEEEEEECCCCEEEEECCCCCCCEEECCCCCEECCCCCEE
VIDLTGPAMVDKDFLLEPREGFGWRLVMDLVPTTPEIFMAGVGQPAKPVVPATVTPEPST
EEEECCCCEECCCCEECCCCCCCEEEEEEECCCCHHHHEECCCCCCCCCCCEEECCCCCC
RAVAFAAAAAPAAAASGTTAPTPIGPGGPVNLRPAAVGKGPVTVNLASLPSAQAPIADPS
CEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
IPGSFGGPGGAAAGEGMQVMAREGPAIVRLADGRRVPIPLEKPQRSVDPVRKPVIVLDPG
CCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCHHHCCEEEEECC
HGGKDPGAIGASGTYEKIVTLEMARQLKRALEATGRYKVVLTRESDTSVRLRERIAFGRH
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCC
AGADLFVSIHADAMANPQVRGLSVYTLSETASDDEAAQLADRENKVDILLGMDLSQESPD
CCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCC
VATILIDLAQRETKNKSVHFANTLVSALPGDVLKLEKTRRYAGFAVLKAPDVPSVLVEMG
CEEHHHHHHHHHCCCCCEEHHHHHHHHCCCCCEEHHHHHHHCCEEEEECCCCHHHHHHHH
FLSNVSDEKLLRTAPYRAKLAAALVRSVDDFFAPQQQRAWKP
HHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHCCCC
>Mature Secondary Structure
MERYVGRGTTPPMARRKATGVSRRSLLAGTPFVAAALLAPRPALAATRATGLRLGDHGGS
CCCCCCCCCCCCHHHHHHCCCCHHHHHCCCHHHHHHHHCCCCHHHHHHHCCEEEECCCCC
TRFVLELSGPVTWQLVSLSAPYRLIIDMPEVEFALGLVPRDTGLVAGARYGVVRPGFSRM
EEEEEEECCCCEEEEEEECCCEEEEEECCCCEEEEECCCCCCCEEECCCCCEECCCCCEE
VIDLTGPAMVDKDFLLEPREGFGWRLVMDLVPTTPEIFMAGVGQPAKPVVPATVTPEPST
EEEECCCCEECCCCEECCCCCCCEEEEEEECCCCHHHHEECCCCCCCCCCCEEECCCCCC
RAVAFAAAAAPAAAASGTTAPTPIGPGGPVNLRPAAVGKGPVTVNLASLPSAQAPIADPS
CEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
IPGSFGGPGGAAAGEGMQVMAREGPAIVRLADGRRVPIPLEKPQRSVDPVRKPVIVLDPG
CCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCHHHCCEEEEECC
HGGKDPGAIGASGTYEKIVTLEMARQLKRALEATGRYKVVLTRESDTSVRLRERIAFGRH
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCC
AGADLFVSIHADAMANPQVRGLSVYTLSETASDDEAAQLADRENKVDILLGMDLSQESPD
CCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCC
VATILIDLAQRETKNKSVHFANTLVSALPGDVLKLEKTRRYAGFAVLKAPDVPSVLVEMG
CEEHHHHHHHHHCCCCCEEHHHHHHHHCCCCCEEHHHHHHHCCEEEEECCCCHHHHHHHH
FLSNVSDEKLLRTAPYRAKLAAALVRSVDDFFAPQQQRAWKP
HHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]